UniProtKB - Q9UPR3 (SMG5_HUMAN)
Nonsense-mediated mRNA decay factor SMG5
SMG5
Functioni
Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.
1 PublicationGO - Molecular functioni
- histone deacetylase binding Source: BHF-UCL
- protein phosphatase 2A binding Source: HGNC-UCL
- telomerase RNA binding Source: BHF-UCL
- telomeric DNA binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- mRNA export from nucleus Source: HGNC-UCL
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: GO_Central
- regulation of dephosphorylation Source: HGNC-UCL
- regulation of telomere maintenance Source: BHF-UCL
- regulation of telomere maintenance via telomerase Source: BHF-UCL
Keywordsi
Biological process | Nonsense-mediated mRNA decay |
Enzyme and pathway databases
PathwayCommonsi | Q9UPR3 |
Reactomei | R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SignaLinki | Q9UPR3 |
Names & Taxonomyi
Protein namesi | Recommended name: Nonsense-mediated mRNA decay factor SMG5ImportedAlternative name(s): EST1-like protein B1 Publication LPTS-RP11 Publication LPTS-interacting protein1 Publication SMG-5 homolog1 Publication Short name: hSMG-51 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24644, SMG5 |
MIMi | 610962, gene |
neXtProti | NX_Q9UPR3 |
VEuPathDBi | HostDB:ENSG00000198952 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: HGNC-UCL
- telomerase holoenzyme complex Source: GO_Central
Other locations
- cytoplasm Source: HGNC-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 860 | D → A: Abolishes stimulation of RENT1 dephosphorylation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23381 |
OpenTargetsi | ENSG00000198952 |
PharmGKBi | PA143485617 |
Miscellaneous databases
Pharosi | Q9UPR3, Tbio |
Genetic variation databases
BioMutai | SMG5 |
DMDMi | 84029494 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000076322 | 2 – 1016 | Nonsense-mediated mRNA decay factor SMG5Add BLAST | 1015 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Modified residuei | 423 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9UPR3 |
jPOSTi | Q9UPR3 |
MassIVEi | Q9UPR3 |
MaxQBi | Q9UPR3 |
PaxDbi | Q9UPR3 |
PeptideAtlasi | Q9UPR3 |
PRIDEi | Q9UPR3 |
ProteomicsDBi | 85426 |
PTM databases
iPTMneti | Q9UPR3 |
PhosphoSitePlusi | Q9UPR3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000198952, Expressed in apex of heart and 237 other tissues |
Genevisiblei | Q9UPR3, HS |
Organism-specific databases
HPAi | ENSG00000198952, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TERT, PPP2CA and SMG1.
Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.
2 PublicationsBinary interactionsi
Q9UPR3
With | #Exp. | IntAct |
---|---|---|
SMG7 [Q92540] | 5 | EBI-3400861,EBI-719830 |
UPF1 - isoform 2 [Q92900-2] | 2 | EBI-3400861,EBI-373492 |
GO - Molecular functioni
- histone deacetylase binding Source: BHF-UCL
- protein phosphatase 2A binding Source: HGNC-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 116957, 73 interactors |
IntActi | Q9UPR3, 14 interactors |
MINTi | Q9UPR3 |
STRINGi | 9606.ENSP00000355261 |
Miscellaneous databases
RNActi | Q9UPR3, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9UPR3 |
SMRi | Q9UPR3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UPR3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 872 – 995 | PINcAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 408 – 561 | DisorderedSequence analysisAdd BLAST | 154 | |
Regioni | 594 – 637 | DisorderedSequence analysisAdd BLAST | 44 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 798 – 841 | Sequence analysisAdd BLAST | 44 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 418 – 436 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 463 – 492 | Basic and acidic residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 515 – 538 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2162, Eukaryota |
GeneTreei | ENSGT00940000154566 |
HOGENOMi | CLU_011872_0_0_1 |
InParanoidi | Q9UPR3 |
OMAi | FQIIECC |
OrthoDBi | 184090at2759 |
PhylomeDBi | Q9UPR3 |
TreeFami | TF327119 |
Family and domain databases
DisProti | DP01878 |
Gene3Di | 1.25.40.10, 1 hit |
IDEALi | IID00107 |
InterProi | View protein in InterPro IPR018834, DNA/RNA-bd_Est1-type IPR045153, Est1/Ebs1-like IPR019458, Est1_N IPR002716, PIN_dom IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR15696, PTHR15696, 1 hit |
Pfami | View protein in Pfam PF10374, EST1, 1 hit PF10373, EST1_DNA_bind, 2 hits PF13638, PIN_4, 1 hit |
SMARTi | View protein in SMART SM00670, PINc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSQGPPTGES SEPEAKVLHT KRLYRAVVEA VHRLDLILCN KTAYQEVFKP
60 70 80 90 100
ENISLRNKLR ELCVKLMFLH PVDYGRKAEE LLWRKVYYEV IQLIKTNKKH
110 120 130 140 150
IHSRSTLECA YRTHLVAGIG FYQHLLLYIQ SHYQLELQCC IDWTHVTDPL
160 170 180 190 200
IGCKKPVSAS GKEMDWAQMA CHRCLVYLGD LSRYQNELAG VDTELLAERF
210 220 230 240 250
YYQALSVAPQ IGMPFNQLGT LAGSKYYNVE AMYCYLRCIQ SEVSFEGAYG
260 270 280 290 300
NLKRLYDKAA KMYHQLKKCE TRKLSPGKKR CKDIKRLLVN FMYLQSLLQP
310 320 330 340 350
KSSSVDSELT SLCQSVLEDF NLCLFYLPSS PNLSLASEDE EEYESGYAFL
360 370 380 390 400
PDLLIFQMVI ICLMCVHSLE RAGSKQYSAA IAFTLALFSH LVNHVNIRLQ
410 420 430 440 450
AELEEGENPV PAFQSDGTDE PESKEPVEKE EEPDPEPPPV TPQVGEGRKS
460 470 480 490 500
RKFSRLSCLR RRRHPPKVGD DSDLSEGFES DSSHDSARAS EGSDSGSDKS
510 520 530 540 550
LEGGGTAFDA ETDSEMNSQE SRSDLEDMEE EEGTRSPTLE PPRGRSEAPD
560 570 580 590 600
SLNGPLGPSE ASIASNLQAM STQMFQTKRC FRLAPTFSNL LLQPTTNPHT
610 620 630 640 650
SASHRPCVNG DVDKPSEPAS EEGSESEGSE SSGRSCRNER SIQEKLQVLM
660 670 680 690 700
AEGLLPAVKV FLDWLRTNPD LIIVCAQSSQ SLWNRLSVLL NLLPAAGELQ
710 720 730 740 750
ESGLALCPEV QDLLEGCELP DLPSSLLLPE DMALRNLPPL RAAHRRFNFD
760 770 780 790 800
TDRPLLSTLE ESVVRICCIR SFGHFIARLQ GSILQFNPEV GIFVSIAQSE
810 820 830 840 850
QESLLQQAQA QFRMAQEEAR RNRLMRDMAQ LRLQLEVSQL EGSLQQPKAQ
860 870 880 890 900
SAMSPYLVPD TQALCHHLPV IRQLATSGRF IVIIPRTVID GLDLLKKEHP
910 920 930 940 950
GARDGIRYLE AEFKKGNRYI RCQKEVGKSF ERHKLKRQDA DAWTLYKILD
960 970 980 990 1000
SCKQLTLAQG AGEEDPSGMV TIITGLPLDN PSVLSGPMQA ALQAAAHASV
1010
DIKNVLDFYK QWKEIG
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 372 | A → S in AAQ84301 (Ref. 3) Curated | 1 | |
Sequence conflicti | 384 | T → A in AAQ84301 (Ref. 3) Curated | 1 | |
Sequence conflicti | 496 | G → R in AAQ84301 (Ref. 3) Curated | 1 | |
Sequence conflicti | 915 | K → KK in CAH56374 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030828 | 1004 | N → D1 PublicationCorresponds to variant dbSNP:rs17853821Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB085691 mRNA Translation: BAC53623.1 AY168922 mRNA Translation: AAO17582.1 AY336728 mRNA Translation: AAQ84301.1 AB029012 mRNA Translation: BAA83041.2 Different initiation. AL589685 Genomic DNA No translation available. AL135927 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW52973.1 CH471121 Genomic DNA Translation: EAW52974.1 BC007453 mRNA Translation: AAH07453.2 BC038296 mRNA Translation: AAH38296.1 AL137738 mRNA Translation: CAH56374.1 |
CCDSi | CCDS1137.1 |
RefSeqi | NP_056142.2, NM_015327.2 |
Genome annotation databases
Ensembli | ENST00000361813.5; ENSP00000355261.5; ENSG00000198952.8 |
GeneIDi | 23381 |
KEGGi | hsa:23381 |
MANE-Selecti | ENST00000361813.5; ENSP00000355261.5; NM_015327.3; NP_056142.2 |
UCSCi | uc001foc.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB085691 mRNA Translation: BAC53623.1 AY168922 mRNA Translation: AAO17582.1 AY336728 mRNA Translation: AAQ84301.1 AB029012 mRNA Translation: BAA83041.2 Different initiation. AL589685 Genomic DNA No translation available. AL135927 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW52973.1 CH471121 Genomic DNA Translation: EAW52974.1 BC007453 mRNA Translation: AAH07453.2 BC038296 mRNA Translation: AAH38296.1 AL137738 mRNA Translation: CAH56374.1 |
CCDSi | CCDS1137.1 |
RefSeqi | NP_056142.2, NM_015327.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HWY | X-ray | 2.75 | A/B | 853-1016 | [»] | |
AlphaFoldDBi | Q9UPR3 | |||||
SMRi | Q9UPR3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116957, 73 interactors |
IntActi | Q9UPR3, 14 interactors |
MINTi | Q9UPR3 |
STRINGi | 9606.ENSP00000355261 |
PTM databases
iPTMneti | Q9UPR3 |
PhosphoSitePlusi | Q9UPR3 |
Genetic variation databases
BioMutai | SMG5 |
DMDMi | 84029494 |
Proteomic databases
EPDi | Q9UPR3 |
jPOSTi | Q9UPR3 |
MassIVEi | Q9UPR3 |
MaxQBi | Q9UPR3 |
PaxDbi | Q9UPR3 |
PeptideAtlasi | Q9UPR3 |
PRIDEi | Q9UPR3 |
ProteomicsDBi | 85426 |
Protocols and materials databases
Antibodypediai | 34209, 108 antibodies from 23 providers |
DNASUi | 23381 |
Genome annotation databases
Ensembli | ENST00000361813.5; ENSP00000355261.5; ENSG00000198952.8 |
GeneIDi | 23381 |
KEGGi | hsa:23381 |
MANE-Selecti | ENST00000361813.5; ENSP00000355261.5; NM_015327.3; NP_056142.2 |
UCSCi | uc001foc.5, human |
Organism-specific databases
CTDi | 23381 |
DisGeNETi | 23381 |
GeneCardsi | SMG5 |
HGNCi | HGNC:24644, SMG5 |
HPAi | ENSG00000198952, Low tissue specificity |
MIMi | 610962, gene |
neXtProti | NX_Q9UPR3 |
OpenTargetsi | ENSG00000198952 |
PharmGKBi | PA143485617 |
VEuPathDBi | HostDB:ENSG00000198952 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2162, Eukaryota |
GeneTreei | ENSGT00940000154566 |
HOGENOMi | CLU_011872_0_0_1 |
InParanoidi | Q9UPR3 |
OMAi | FQIIECC |
OrthoDBi | 184090at2759 |
PhylomeDBi | Q9UPR3 |
TreeFami | TF327119 |
Enzyme and pathway databases
PathwayCommonsi | Q9UPR3 |
Reactomei | R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SignaLinki | Q9UPR3 |
Miscellaneous databases
BioGRID-ORCSi | 23381, 617 hits in 1093 CRISPR screens |
ChiTaRSi | SMG5, human |
EvolutionaryTracei | Q9UPR3 |
GeneWikii | SMG5 |
GenomeRNAii | 23381 |
Pharosi | Q9UPR3, Tbio |
PROi | PR:Q9UPR3 |
RNActi | Q9UPR3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000198952, Expressed in apex of heart and 237 other tissues |
Genevisiblei | Q9UPR3, HS |
Family and domain databases
DisProti | DP01878 |
Gene3Di | 1.25.40.10, 1 hit |
IDEALi | IID00107 |
InterProi | View protein in InterPro IPR018834, DNA/RNA-bd_Est1-type IPR045153, Est1/Ebs1-like IPR019458, Est1_N IPR002716, PIN_dom IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR15696, PTHR15696, 1 hit |
Pfami | View protein in Pfam PF10374, EST1, 1 hit PF10373, EST1_DNA_bind, 2 hits PF13638, PIN_4, 1 hit |
SMARTi | View protein in SMART SM00670, PINc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMG5_HUMAN | |
Accessioni | Q9UPR3Primary (citable) accession number: Q9UPR3 Secondary accession number(s): D3DVB7 Q96IJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references