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Protein

Protein SMG5

Gene

SMG5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.1 Publication

GO - Molecular functioni

  • histone deacetylase binding Source: BHF-UCL
  • protein phosphatase 2A binding Source: HGNC
  • ribonuclease activity Source: GO_Central
  • ribonucleoprotein complex binding Source: GO_Central
  • telomerase RNA binding Source: BHF-UCL
  • telomeric DNA binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processNonsense-mediated mRNA decay

Enzyme and pathway databases

ReactomeiR-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SMG5
Alternative name(s):
EST1-like protein B
LPTS-RP1
LPTS-interacting protein
SMG-5 homolog
Short name:
hSMG-5
Gene namesi
Name:SMG5
Synonyms:EST1B, KIAA1089
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000198952.8
HGNCiHGNC:24644 SMG5
MIMi610962 gene
neXtProtiNX_Q9UPR3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi860D → A: Abolishes stimulation of RENT1 dephosphorylation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000198952
PharmGKBiPA143485617

Polymorphism and mutation databases

BioMutaiSMG5
DMDMi84029494

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000763222 – 1016Protein SMG5Add BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei423PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UPR3
MaxQBiQ9UPR3
PaxDbiQ9UPR3
PeptideAtlasiQ9UPR3
PRIDEiQ9UPR3
ProteomicsDBi85426

PTM databases

iPTMnetiQ9UPR3
PhosphoSitePlusiQ9UPR3

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiENSG00000198952 Expressed in 222 organ(s), highest expression level in apex of heart
CleanExiHS_SMG5
GenevisibleiQ9UPR3 HS

Organism-specific databases

HPAiHPA072651

Interactioni

Subunit structurei

Interacts with TERT, PPP2CA and SMG1. Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116957, 17 interactors
IntActiQ9UPR3, 8 interactors
MINTiQ9UPR3
STRINGi9606.ENSP00000355261

Structurei

Secondary structure

11016
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UPR3
SMRiQ9UPR3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UPR3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini872 – 995PINcAdd BLAST124

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili798 – 841Sequence analysisAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi460 – 463Poly-Arg4
Compositional biasi529 – 532Poly-Glu4

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INQY Eukaryota
ENOG410XPF4 LUCA
GeneTreeiENSGT00560000077120
HOVERGENiHBG082268
InParanoidiQ9UPR3
KOiK11125
OMAiGMAQNQL
OrthoDBiEOG091G0188
PhylomeDBiQ9UPR3
TreeFamiTF327119

Family and domain databases

InterProiView protein in InterPro
IPR018834 DNA/RNA-bd_Est1-type
IPR019458 EST1
IPR002716 PIN_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF10374 EST1, 1 hit
PF10373 EST1_DNA_bind, 2 hits
PF13638 PIN_4, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UPR3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQGPPTGES SEPEAKVLHT KRLYRAVVEA VHRLDLILCN KTAYQEVFKP
60 70 80 90 100
ENISLRNKLR ELCVKLMFLH PVDYGRKAEE LLWRKVYYEV IQLIKTNKKH
110 120 130 140 150
IHSRSTLECA YRTHLVAGIG FYQHLLLYIQ SHYQLELQCC IDWTHVTDPL
160 170 180 190 200
IGCKKPVSAS GKEMDWAQMA CHRCLVYLGD LSRYQNELAG VDTELLAERF
210 220 230 240 250
YYQALSVAPQ IGMPFNQLGT LAGSKYYNVE AMYCYLRCIQ SEVSFEGAYG
260 270 280 290 300
NLKRLYDKAA KMYHQLKKCE TRKLSPGKKR CKDIKRLLVN FMYLQSLLQP
310 320 330 340 350
KSSSVDSELT SLCQSVLEDF NLCLFYLPSS PNLSLASEDE EEYESGYAFL
360 370 380 390 400
PDLLIFQMVI ICLMCVHSLE RAGSKQYSAA IAFTLALFSH LVNHVNIRLQ
410 420 430 440 450
AELEEGENPV PAFQSDGTDE PESKEPVEKE EEPDPEPPPV TPQVGEGRKS
460 470 480 490 500
RKFSRLSCLR RRRHPPKVGD DSDLSEGFES DSSHDSARAS EGSDSGSDKS
510 520 530 540 550
LEGGGTAFDA ETDSEMNSQE SRSDLEDMEE EEGTRSPTLE PPRGRSEAPD
560 570 580 590 600
SLNGPLGPSE ASIASNLQAM STQMFQTKRC FRLAPTFSNL LLQPTTNPHT
610 620 630 640 650
SASHRPCVNG DVDKPSEPAS EEGSESEGSE SSGRSCRNER SIQEKLQVLM
660 670 680 690 700
AEGLLPAVKV FLDWLRTNPD LIIVCAQSSQ SLWNRLSVLL NLLPAAGELQ
710 720 730 740 750
ESGLALCPEV QDLLEGCELP DLPSSLLLPE DMALRNLPPL RAAHRRFNFD
760 770 780 790 800
TDRPLLSTLE ESVVRICCIR SFGHFIARLQ GSILQFNPEV GIFVSIAQSE
810 820 830 840 850
QESLLQQAQA QFRMAQEEAR RNRLMRDMAQ LRLQLEVSQL EGSLQQPKAQ
860 870 880 890 900
SAMSPYLVPD TQALCHHLPV IRQLATSGRF IVIIPRTVID GLDLLKKEHP
910 920 930 940 950
GARDGIRYLE AEFKKGNRYI RCQKEVGKSF ERHKLKRQDA DAWTLYKILD
960 970 980 990 1000
SCKQLTLAQG AGEEDPSGMV TIITGLPLDN PSVLSGPMQA ALQAAAHASV
1010
DIKNVLDFYK QWKEIG
Length:1,016
Mass (Da):113,928
Last modified:December 20, 2005 - v3
Checksum:iB6F8F9B53CF42195
GO

Sequence cautioni

The sequence BAA83041 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti372A → S in AAQ84301 (Ref. 3) Curated1
Sequence conflicti384T → A in AAQ84301 (Ref. 3) Curated1
Sequence conflicti496G → R in AAQ84301 (Ref. 3) Curated1
Sequence conflicti915K → KK in CAH56374 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0308281004N → D1 PublicationCorresponds to variant dbSNP:rs17853821Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085691 mRNA Translation: BAC53623.1
AY168922 mRNA Translation: AAO17582.1
AY336728 mRNA Translation: AAQ84301.1
AB029012 mRNA Translation: BAA83041.2 Different initiation.
AL589685 Genomic DNA No translation available.
AL135927 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW52973.1
CH471121 Genomic DNA Translation: EAW52974.1
BC007453 mRNA Translation: AAH07453.2
BC038296 mRNA Translation: AAH38296.1
AL137738 mRNA Translation: CAH56374.1
CCDSiCCDS1137.1
RefSeqiNP_056142.2, NM_015327.2
UniGeneiHs.516837

Genome annotation databases

EnsembliENST00000361813; ENSP00000355261; ENSG00000198952
GeneIDi23381
KEGGihsa:23381
UCSCiuc001foc.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085691 mRNA Translation: BAC53623.1
AY168922 mRNA Translation: AAO17582.1
AY336728 mRNA Translation: AAQ84301.1
AB029012 mRNA Translation: BAA83041.2 Different initiation.
AL589685 Genomic DNA No translation available.
AL135927 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW52973.1
CH471121 Genomic DNA Translation: EAW52974.1
BC007453 mRNA Translation: AAH07453.2
BC038296 mRNA Translation: AAH38296.1
AL137738 mRNA Translation: CAH56374.1
CCDSiCCDS1137.1
RefSeqiNP_056142.2, NM_015327.2
UniGeneiHs.516837

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HWYX-ray2.75A/B853-1016[»]
ProteinModelPortaliQ9UPR3
SMRiQ9UPR3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116957, 17 interactors
IntActiQ9UPR3, 8 interactors
MINTiQ9UPR3
STRINGi9606.ENSP00000355261

PTM databases

iPTMnetiQ9UPR3
PhosphoSitePlusiQ9UPR3

Polymorphism and mutation databases

BioMutaiSMG5
DMDMi84029494

Proteomic databases

EPDiQ9UPR3
MaxQBiQ9UPR3
PaxDbiQ9UPR3
PeptideAtlasiQ9UPR3
PRIDEiQ9UPR3
ProteomicsDBi85426

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361813; ENSP00000355261; ENSG00000198952
GeneIDi23381
KEGGihsa:23381
UCSCiuc001foc.5 human

Organism-specific databases

CTDi23381
EuPathDBiHostDB:ENSG00000198952.8
GeneCardsiSMG5
HGNCiHGNC:24644 SMG5
HPAiHPA072651
MIMi610962 gene
neXtProtiNX_Q9UPR3
OpenTargetsiENSG00000198952
PharmGKBiPA143485617
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INQY Eukaryota
ENOG410XPF4 LUCA
GeneTreeiENSGT00560000077120
HOVERGENiHBG082268
InParanoidiQ9UPR3
KOiK11125
OMAiGMAQNQL
OrthoDBiEOG091G0188
PhylomeDBiQ9UPR3
TreeFamiTF327119

Enzyme and pathway databases

ReactomeiR-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSiSMG5 human
EvolutionaryTraceiQ9UPR3
GeneWikiiSMG5
GenomeRNAii23381
PROiPR:Q9UPR3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198952 Expressed in 222 organ(s), highest expression level in apex of heart
CleanExiHS_SMG5
GenevisibleiQ9UPR3 HS

Family and domain databases

InterProiView protein in InterPro
IPR018834 DNA/RNA-bd_Est1-type
IPR019458 EST1
IPR002716 PIN_dom
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF10374 EST1, 1 hit
PF10373 EST1_DNA_bind, 2 hits
PF13638 PIN_4, 1 hit
SMARTiView protein in SMART
SM00670 PINc, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMG5_HUMAN
AccessioniPrimary (citable) accession number: Q9UPR3
Secondary accession number(s): D3DVB7
, Q5QJE7, Q659C7, Q8IXC0, Q8IY09, Q96IJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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