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Protein

Protein SCAF8

Gene

SCAF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in mRNA processing.1 Publication

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • RNA polymerase core enzyme binding Source: UniProtKB
  • RNA polymerase II complex binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SCAF8
Alternative name(s):
CDC5L complex-associated protein 7
RNA-binding motif protein 16
SR-related and CTD-associated factor 8
Gene namesi
Name:SCAF8
Synonyms:CCAP7, KIAA1116, RBM16
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000213079.9
HGNCiHGNC:20959 SCAF8
MIMi616024 gene
neXtProtiNX_Q9UPN6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000213079
PharmGKBiPA128394587

Polymorphism and mutation databases

BioMutaiSCAF8
DMDMi30580495

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817801 – 1271Protein SCAF8Add BLAST1271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6Phosphothreonine1 Publication1
Cross-linki18Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)
Modified residuei273PhosphoserineCombined sources1
Modified residuei615PhosphothreonineCombined sources1
Modified residuei617PhosphoserineCombined sources1
Modified residuei779PhosphoserineCombined sources1
Modified residuei917Asymmetric dimethylarginineCombined sources1
Modified residuei927Asymmetric dimethylarginineCombined sources1
Modified residuei938Asymmetric dimethylarginineCombined sources1
Modified residuei1073Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UPN6
PaxDbiQ9UPN6
PeptideAtlasiQ9UPN6
PRIDEiQ9UPN6
ProteomicsDBi85390

PTM databases

iPTMnetiQ9UPN6
PhosphoSitePlusiQ9UPN6

Expressioni

Gene expression databases

BgeeiENSG00000213079 Expressed in 237 organ(s), highest expression level in corpus callosum
CleanExiHS_RBM16
ExpressionAtlasiQ9UPN6 baseline and differential
GenevisibleiQ9UPN6 HS

Organism-specific databases

HPAiHPA035601
HPA035602

Interactioni

Subunit structurei

Interacts with POLR2A (via C-terminus). Has much higher affinity for phosphorylated POLR2A. Identified in a complex with CDC5L and other spliceosomal proteins. May associate with the spliceosome.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083793EBI-7954236,EBI-618309

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116503, 24 interactors
CORUMiQ9UPN6
IntActiQ9UPN6, 10 interactors
MINTiQ9UPN6
STRINGi9606.ENSP00000356146

Structurei

Secondary structure

11271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UPN6
SMRiQ9UPN6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UPN6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 139CIDPROSITE-ProRule annotationAdd BLAST139
Domaini477 – 551RRMPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi193 – 371Gln-richAdd BLAST179
Compositional biasi397 – 459Arg-richAdd BLAST63
Compositional biasi635 – 1064Pro-richAdd BLAST430
Compositional biasi1120 – 1182Arg-richAdd BLAST63

Phylogenomic databases

eggNOGiKOG0132 Eukaryota
ENOG410XT48 LUCA
GeneTreeiENSGT00530000063946
HOGENOMiHOG000154078
HOVERGENiHBG055796
InParanoidiQ9UPN6
KOiK13191
PhylomeDBiQ9UPN6
TreeFamiTF324527

Family and domain databases

CDDicd03562 CID, 1 hit
cd12462 RRM_SCAF8, 1 hit
Gene3Di1.25.40.90, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR006569 CID_dom
IPR008942 ENTH_VHS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR006903 RNA_pol_II-bd
IPR000504 RRM_dom
IPR034370 SCAF8_RRM
PfamiView protein in Pfam
PF04818 CTD_bind, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00582 RPR, 1 hit
SM00360 RRM, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS51391 CID, 1 hit
PS50102 RRM, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UPN6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAVKTFNSE LYSLNDYKPP ISKAKMTQIT KAAIKAIKFY KHVVQSVEKF
60 70 80 90 100
IQKCKPEYKV PGLYVIDSIV RQSRHQFGQE KDVFAPRFSN NIISTFQNLY
110 120 130 140 150
RCPGDDKSKI VRVLNLWQKN NVFKSEIIQP LLDMAAGIPP PVVTPVLAST
160 170 180 190 200
TTAMSNTPGT PVTPVTPANV VQGLPDPWVS QITNTDTLAA VAQILQSPQG
210 220 230 240 250
QQLQQLIQTL QIQQQKPQPS ILQALDAGLV VQLQALTAQL TAAAAAANTL
260 270 280 290 300
TPLEQGVSFN KKLMDRFDFG EDSEHSEEPK KEIPASQLSH VSESVNNSIF
310 320 330 340 350
HQIAEQLQQQ NLEHLRQQLL EQQQPQKATP QDSQEGTFGS EHSASPSQGS
360 370 380 390 400
SQQHFLEPEV NLDDSIDIQQ QDMDIDEGQD GVEEEVFEQE AKKVAVRSRS
410 420 430 440 450
RTHSRSRSRS PRKRRSRSRS GSRKRKHRKR SRSRSRERKR KSSRSYSSER
460 470 480 490 500
RAREREKERQ KKGLPPIRSK TLSVCSTTLW VGQVDKKATQ QDLTNLFEEF
510 520 530 540 550
GQIESINMIP PRGCAYVCMV HRQDAFRALQ KLSSGSYKIG SKVIKIAWAL
560 570 580 590 600
NKGVKTEYKQ FWDVDLGVTY IPWEKVKVDD LEGFAEGGMI DQETVNTEWE
610 620 630 640 650
TVKSSEPVKE TVQTTQSPTP VEKETVVTTQ AEVFPPPVAM LQIPVAPAVP
660 670 680 690 700
TVSLVPPAFP VSMPVPPPGF SPIPPPPFLR ASFNPSQPPP GFMPPPVPPP
710 720 730 740 750
VVPPPTIPPV VPTSLVQPSL SMTPETVKDV GFGSLVIPGG SVASNLATSA
760 770 780 790 800
LPAGNVFNAP TKQAEPEEKV PHLIDHQISS GENTRSVIPN DISSNAAILG
810 820 830 840 850
GQPPNVTSNS GILGVQRPNV SSNSEILGVR PSNVSSSSGI IAAQPPNILN
860 870 880 890 900
NSGILGIQPP SVSNSSGLLG VLPPNIPNNS GLVGVQPPNV PNTPGLLGTQ
910 920 930 940 950
PPAGPQNLPP LSIPNQRMPT MPMLDIRPGL IPQAPGPRFP LIQPGIPPQR
960 970 980 990 1000
GIPPPSVLDS ALHPPPRGPF PPGDIFSQPE RPFLAPGRQS VDNVTNPEKR
1010 1020 1030 1040 1050
IPLGNDNIQQ EGDRDYRFPP IETRESISRP PPVDVRDVVG RPIDPREGPG
1060 1070 1080 1090 1100
RPPLDGRDHF GRPPVDIREN LVRPGIDHLG RRDHFGFNPE KPWGHRGDFD
1110 1120 1130 1140 1150
EREHRVLPVY GGPKGLHEER GRFRSGNYRF DPRSGPWNRG FGQEVHRDFD
1160 1170 1180 1190 1200
DRRRPWERQR DRDDRDFDFC REMNGNRLGR DRIQNTWVPP PHARVFDYFE
1210 1220 1230 1240 1250
GATSQRKGDN VPQVNGENTE RHAQPPPIPV QNDPELYEKL TSSNEINKEK
1260 1270
SDTVADIESE PVVESTETEG T
Length:1,271
Mass (Da):140,519
Last modified:May 1, 2000 - v1
Checksum:i9856758DB7622319
GO
Isoform 2 (identifier: Q9UPN6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPQPLLPALPPLSSPSGAGSCGPSGGECSGRRLFRRRARGLRSDNM
     10-10: E → EDCASTPACTDCFSSSMDTRSI

Note: No experimental confirmation available.
Show »
Length:1,337
Mass (Da):147,345
Checksum:i9E5DC6D4A9329466
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MT33A0A0A0MT33_HUMAN
Protein SCAF8
SCAF8
1,349Annotation score:

Sequence cautioni

The sequence BAA83068 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297N → I in AAH70071 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052220865S → N. Corresponds to variant dbSNP:rs34802160Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561491M → MPQPLLPALPPLSSPSGAGS CGPSGGECSGRRLFRRRARG LRSDNM in isoform 2. 1 Publication1
Alternative sequenceiVSP_05615010E → EDCASTPACTDCFSSSMDTR SI in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029039 mRNA Translation: BAA83068.2 Different initiation.
AK303001 mRNA Translation: BAH13874.1
AL121952 Genomic DNA No translation available.
AL136976 Genomic DNA No translation available.
AL591499 Genomic DNA No translation available.
BC070071 mRNA Translation: AAH70071.1
CCDSiCCDS5247.1 [Q9UPN6-1]
CCDS69226.1 [Q9UPN6-2]
RefSeqiNP_001273117.1, NM_001286188.1
NP_001273118.1, NM_001286189.1 [Q9UPN6-2]
NP_001273123.1, NM_001286194.1
NP_001273128.1, NM_001286199.1 [Q9UPN6-1]
NP_055707.3, NM_014892.4 [Q9UPN6-1]
UniGeneiHs.591329

Genome annotation databases

EnsembliENST00000367178; ENSP00000356146; ENSG00000213079 [Q9UPN6-1]
ENST00000367186; ENSP00000356154; ENSG00000213079 [Q9UPN6-2]
GeneIDi22828
KEGGihsa:22828
UCSCiuc003qpz.5 human [Q9UPN6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029039 mRNA Translation: BAA83068.2 Different initiation.
AK303001 mRNA Translation: BAH13874.1
AL121952 Genomic DNA No translation available.
AL136976 Genomic DNA No translation available.
AL591499 Genomic DNA No translation available.
BC070071 mRNA Translation: AAH70071.1
CCDSiCCDS5247.1 [Q9UPN6-1]
CCDS69226.1 [Q9UPN6-2]
RefSeqiNP_001273117.1, NM_001286188.1
NP_001273118.1, NM_001286189.1 [Q9UPN6-2]
NP_001273123.1, NM_001286194.1
NP_001273128.1, NM_001286199.1 [Q9UPN6-1]
NP_055707.3, NM_014892.4 [Q9UPN6-1]
UniGeneiHs.591329

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DIWNMR-A1-137[»]
3D9IX-ray1.91A/B1-136[»]
3D9JX-ray1.60A/B1-136[»]
3D9KX-ray2.20A/B1-136[»]
3D9LX-ray2.20A/B1-136[»]
3D9MX-ray1.75A/B1-136[»]
3D9NX-ray1.60A/B1-136[»]
3D9OX-ray2.00A/B1-136[»]
3D9PX-ray2.10A/B1-136[»]
ProteinModelPortaliQ9UPN6
SMRiQ9UPN6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116503, 24 interactors
CORUMiQ9UPN6
IntActiQ9UPN6, 10 interactors
MINTiQ9UPN6
STRINGi9606.ENSP00000356146

PTM databases

iPTMnetiQ9UPN6
PhosphoSitePlusiQ9UPN6

Polymorphism and mutation databases

BioMutaiSCAF8
DMDMi30580495

Proteomic databases

EPDiQ9UPN6
PaxDbiQ9UPN6
PeptideAtlasiQ9UPN6
PRIDEiQ9UPN6
ProteomicsDBi85390

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367178; ENSP00000356146; ENSG00000213079 [Q9UPN6-1]
ENST00000367186; ENSP00000356154; ENSG00000213079 [Q9UPN6-2]
GeneIDi22828
KEGGihsa:22828
UCSCiuc003qpz.5 human [Q9UPN6-1]

Organism-specific databases

CTDi22828
EuPathDBiHostDB:ENSG00000213079.9
GeneCardsiSCAF8
HGNCiHGNC:20959 SCAF8
HPAiHPA035601
HPA035602
MIMi616024 gene
neXtProtiNX_Q9UPN6
OpenTargetsiENSG00000213079
PharmGKBiPA128394587
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0132 Eukaryota
ENOG410XT48 LUCA
GeneTreeiENSGT00530000063946
HOGENOMiHOG000154078
HOVERGENiHBG055796
InParanoidiQ9UPN6
KOiK13191
PhylomeDBiQ9UPN6
TreeFamiTF324527

Miscellaneous databases

ChiTaRSiSCAF8 human
EvolutionaryTraceiQ9UPN6
GenomeRNAii22828
PROiPR:Q9UPN6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000213079 Expressed in 237 organ(s), highest expression level in corpus callosum
CleanExiHS_RBM16
ExpressionAtlasiQ9UPN6 baseline and differential
GenevisibleiQ9UPN6 HS

Family and domain databases

CDDicd03562 CID, 1 hit
cd12462 RRM_SCAF8, 1 hit
Gene3Di1.25.40.90, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR006569 CID_dom
IPR008942 ENTH_VHS
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR006903 RNA_pol_II-bd
IPR000504 RRM_dom
IPR034370 SCAF8_RRM
PfamiView protein in Pfam
PF04818 CTD_bind, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00582 RPR, 1 hit
SM00360 RRM, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS51391 CID, 1 hit
PS50102 RRM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCAF8_HUMAN
AccessioniPrimary (citable) accession number: Q9UPN6
Secondary accession number(s): B7Z888
, Q5TBU6, Q6NSK3, Q9BQN8, Q9BX43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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