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Protein

NSFL1 cofactor p47

Gene

NSFL1C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed:15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • establishment of mitotic spindle orientation Source: UniProtKB
  • Golgi organization Source: GO_Central
  • membrane fusion Source: GO_Central
  • negative regulation of protein localization to centrosome Source: UniProtKB
  • nuclear envelope reassembly Source: GO_Central
  • positive regulation of mitotic centrosome separation Source: UniProtKB
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central

Keywordsi

LigandLipid-binding

Enzyme and pathway databases

SIGNORiQ9UNZ2

Names & Taxonomyi

Protein namesi
Recommended name:
NSFL1 cofactor p47
Alternative name(s):
UBX domain-containing protein 2C
p97 cofactor p47
Gene namesi
Name:NSFL1C
Synonyms:UBXN2C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000088833.17
HGNCiHGNC:15912 NSFL1C
MIMi606610 gene
neXtProtiNX_Q9UNZ2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55968
OpenTargetsiENSG00000088833
PharmGKBiPA31794

Polymorphism and mutation databases

BioMutaiNSFL1C
DMDMi41017512

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109881 – 370NSFL1 cofactor p47Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei167PhosphotyrosineBy similarity1
Modified residuei176PhosphoserineBy similarity1
Modified residuei192PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UNZ2
PaxDbiQ9UNZ2
PeptideAtlasiQ9UNZ2
PRIDEiQ9UNZ2
ProteomicsDBi85346
85347 [Q9UNZ2-4]
85348 [Q9UNZ2-5]
85349 [Q9UNZ2-6]
TopDownProteomicsiQ9UNZ2-1 [Q9UNZ2-1]
Q9UNZ2-4 [Q9UNZ2-4]
Q9UNZ2-5 [Q9UNZ2-5]

2D gel databases

REPRODUCTION-2DPAGEiIPI00100197

PTM databases

iPTMnetiQ9UNZ2
PhosphoSitePlusiQ9UNZ2

Expressioni

Gene expression databases

BgeeiENSG00000088833
CleanExiHS_NSFL1C
ExpressionAtlasiQ9UNZ2 baseline and differential
GenevisibleiQ9UNZ2 HS

Organism-specific databases

HPAiHPA047108
HPA050628

Interactioni

Subunit structurei

Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121014, 83 interactors
ComplexPortaliCPX-262 NSFL1C-VCP complex
DIPiDIP-39611N
IntActiQ9UNZ2, 25 interactors
MINTiQ9UNZ2
STRINGi9606.ENSP00000418529

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi171 – 173Combined sources3
Beta strandi180 – 193Combined sources14
Beta strandi198 – 200Combined sources3
Turni204 – 206Combined sources3
Helixi207 – 215Combined sources9
Helixi220 – 223Combined sources4
Beta strandi231 – 239Combined sources9
Beta strandi245 – 250Combined sources6
Beta strandi255 – 259Combined sources5
Helixi260 – 262Combined sources3
Beta strandi265 – 268Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SS6NMR-A171-270[»]
ProteinModelPortaliQ9UNZ2
SMRiQ9UNZ2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNZ2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 244SEPPROSITE-ProRule annotationAdd BLAST66
Domaini291 – 368UBXPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 115Nuclear localization signal7
Motifi172 – 175Nuclear localization signal4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi84 – 90Poly-Glu7

Sequence similaritiesi

Belongs to the NSFL1C family.Curated

Phylogenomic databases

eggNOGiKOG2086 Eukaryota
ENOG410YGXU LUCA
GeneTreeiENSGT00520000055567
HOVERGENiHBG054517
InParanoidiQ9UNZ2
KOiK14012
OMAiMRGEIPQ
OrthoDBiEOG091G0QFZ
PhylomeDBiQ9UNZ2
TreeFamiTF312973

Family and domain databases

Gene3Di3.30.420.210, 1 hit
InterProiView protein in InterPro
IPR036241 NSFL1C_SEP_dom_sf
IPR012989 SEP_domain
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR001012 UBX_dom
PfamiView protein in Pfam
PF08059 SEP, 1 hit
PF00789 UBX, 1 hit
SMARTiView protein in SMART
SM00553 SEP, 1 hit
SM00166 UBX, 1 hit
SUPFAMiSSF102848 SSF102848, 1 hit
SSF46934 SSF46934, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS51399 SEP, 1 hit
PS50033 UBX, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UNZ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI
60 70 80 90 100
VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRFYAGGSE
110 120 130 140 150
RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF
160 170 180 190 200
AGGGYRLGAA PEEESAYVAG EKRQHSSQDV HVVLKLWKSG FSLDNGELRS
210 220 230 240 250
YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF
260 270 280 290 300
KAFTGEGQKL GSTAPQVLST SSPAQQAENE AKASSSILID ESEPTTNIQI
310 320 330 340 350
RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFILM TTFPNKELAD
360 370
ESQTLKEANL LNAVIVQRLT
Length:370
Mass (Da):40,573
Last modified:January 16, 2004 - v2
Checksum:i79364617F940B9F9
GO
Isoform 2 (identifier: Q9UNZ2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-179: Missing.

Note: No experimental confirmation available.
Show »
Length:339
Mass (Da):37,325
Checksum:iCF165EC48BB54B96
GO
Isoform 3 (identifier: Q9UNZ2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-93: R → RSR

Show »
Length:372
Mass (Da):40,816
Checksum:i4EFCFE27D995031F
GO
Isoform 4 (identifier: Q9UNZ2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: MAAERQEALR...PNELVDDLFK → MTKMKMRRKRKAR

Show »
Length:259
Mass (Da):28,522
Checksum:i5400E8D45F25448A
GO

Sequence cautioni

The sequence AAF17199 differs from that shown. Sequencing errors.Curated
The sequence AAF17199 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39 – 40AS → EL in AAP97139 (Ref. 1) Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017481290D → N1 PublicationCorresponds to variant dbSNP:rs9575Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0410621 – 124MAAER…DDLFK → MTKMKMRRKRKAR in isoform 4. 1 PublicationAdd BLAST124
Alternative sequenceiVSP_00926293R → RSR in isoform 3. 1 Publication1
Alternative sequenceiVSP_009263149 – 179Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086909 mRNA Translation: AAP97139.1
AF112211 mRNA Translation: AAF17199.1 Sequence problems.
AF078856 mRNA Translation: AAD44488.1
AK297403 mRNA Translation: BAG59842.1
AK001511 mRNA Translation: BAA91731.1
AK315433 mRNA Translation: BAG37821.1
AL109658 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10629.1
CH471133 Genomic DNA Translation: EAX10631.1
BC002801 mRNA Translation: AAH02801.1
CCDSiCCDS13015.1 [Q9UNZ2-1]
CCDS13016.1 [Q9UNZ2-4]
CCDS56175.1 [Q9UNZ2-5]
RefSeqiNP_001193665.1, NM_001206736.1 [Q9UNZ2-5]
NP_057227.2, NM_016143.4 [Q9UNZ2-1]
NP_061327.2, NM_018839.4 [Q9UNZ2-4]
XP_006723657.1, XM_006723594.2 [Q9UNZ2-6]
UniGeneiHs.12865

Genome annotation databases

EnsembliENST00000216879; ENSP00000216879; ENSG00000088833 [Q9UNZ2-1]
ENST00000353088; ENSP00000338643; ENSG00000088833 [Q9UNZ2-4]
ENST00000476071; ENSP00000418529; ENSG00000088833 [Q9UNZ2-5]
GeneIDi55968
KEGGihsa:55968
UCSCiuc002wfc.3 human [Q9UNZ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNSF1C_HUMAN
AccessioniPrimary (citable) accession number: Q9UNZ2
Secondary accession number(s): A2A2L1
, B2RD74, Q5JXA4, Q5JXA5, Q7Z533, Q9H102, Q9NVL9, Q9UI06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: June 20, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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