UniProtKB - Q9UNP9 (PPIE_HUMAN)
Peptidyl-prolyl cis-trans isomerase E
PPIE
Functioni
Catalytic activityi
- EC:5.2.1.86 Publications
Activity regulationi
GO - Molecular functioni
- cyclosporin A binding Source: UniProtKB
- mRNA binding Source: UniProtKB
- peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB
- poly(A) binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- neutrophil degranulation Source: Reactome
- positive regulation of viral genome replication Source: UniProtKB
- protein folding Source: GO_Central
- protein peptidyl-prolyl isomerization Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- transcription-coupled nucleotide-excision repair Source: Reactome
Keywordsi
Molecular function | Isomerase, RNA-binding, Rotamase |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
BRENDAi | 5.2.1.8, 2681 |
PathwayCommonsi | Q9UNP9 |
Reactomei | R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6798695, Neutrophil degranulation R-HSA-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidyl-prolyl cis-trans isomerase E (EC:5.2.1.84 Publications)Short name: PPIase E Alternative name(s): Cyclophilin E Cyclophilin-331 Publication Rotamase E |
Gene namesi | Name:PPIE Synonyms:CYP331 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9258, PPIE |
MIMi | 602435, gene |
neXtProti | NX_Q9UNP9 |
VEuPathDBi | HostDB:ENSG00000084072.16 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular region Source: Reactome
Nucleus
- catalytic step 2 spliceosome Source: UniProtKB
- nuclear speck Source: HPA
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- U2-type catalytic step 2 spliceosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- ficolin-1-rich granule lumen Source: Reactome
- secretory granule lumen Source: Reactome
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | Y → A: Decreased affinity for RNA. 1 Publication | 1 | |
Mutagenesisi | 39 | L → A: Decreased affinity for KMT2A. 2 Publications | 1 | |
Mutagenesisi | 40 – 45 | Missing : Abolishes interaction with KMT2A. 1 Publication | 6 | |
Mutagenesisi | 42 | E → A: Slightly decreased affinity for KMT2A. 1 Publication | 1 | |
Mutagenesisi | 45 | K → A: No effect on interaction with KMT2A. 1 Publication | 1 | |
Mutagenesisi | 47 | R → A: No effect on interaction with KMT2A. 2 Publications | 1 | |
Mutagenesisi | 49 | F → A: Strongly decreased affinity for KMT2A. Decreased affinity for RNA. 2 Publications | 1 | |
Mutagenesisi | 51 | F → A: Impairs protein folding. 2 Publications | 1 | |
Mutagenesisi | 51 | F → D: Abolishes interaction with KMT2A. Abolishes inhibition of KMT2A activity. 1 Publication | 1 | |
Mutagenesisi | 191 | R → A: Expected to disrupt proline isomerase activity. Abolishes inhibition of KMT2A activity; when associated with A-196. 1 Publication | 1 | |
Mutagenesisi | 196 – 197 | FM → EE: Expected to disrupt peptidylproline binding. Decreases interaction with KMT2A. Abolishes inhibition of KMT2A activity. 1 Publication | 2 | |
Mutagenesisi | 196 | F → A: Expected to disrupt proline isomerase activity. Abolishes inhibition of KMT2A activity; when associated with A-191. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10450 |
OpenTargetsi | ENSG00000084072 |
PharmGKBi | PA33583 |
Miscellaneous databases
Pharosi | Q9UNP9, Tbio |
Genetic variation databases
BioMutai | PPIE |
DMDMi | 13124097 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064157 | 1 – 301 | Peptidyl-prolyl cis-trans isomerase EAdd BLAST | 301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphoserineCombined sources | 1 | |
Modified residuei | 97 | PhosphoserineCombined sources | 1 | |
Modified residuei | 119 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9UNP9 |
jPOSTi | Q9UNP9 |
MassIVEi | Q9UNP9 |
PeptideAtlasi | Q9UNP9 |
PRIDEi | Q9UNP9 |
ProteomicsDBi | 65110 85321 [Q9UNP9-1] 85322 [Q9UNP9-2] |
TopDownProteomicsi | Q9UNP9-1 [Q9UNP9-1] Q9UNP9-2 [Q9UNP9-2] |
PTM databases
iPTMneti | Q9UNP9 |
MetOSitei | Q9UNP9 |
PhosphoSitePlusi | Q9UNP9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000084072, Expressed in right testis and 242 other tissues |
ExpressionAtlasi | Q9UNP9, baseline and differential |
Genevisiblei | Q9UNP9, HS |
Organism-specific databases
HPAi | ENSG00000084072, Low tissue specificity |
Interactioni
Subunit structurei
Identified in the spliceosome C complex (PubMed:11991638, PubMed:28076346).
Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE (PubMed:17981804).
Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex (PubMed:25599396). The IB complex does not contain PRPF19 (PubMed:25599396).
Interacts (via RNA-binding domain) with KMT2A (via the third PHD-type zinc-finger) (PubMed:20677832, PubMed:20541251, PubMed:20460131).
7 PublicationsBinary interactionsi
Hide detailsQ9UNP9
With | #Exp. | IntAct |
---|---|---|
AQR [O60306] | 9 | EBI-591818,EBI-2512328 |
FGFR3 [P22607] | 3 | EBI-591818,EBI-348399 |
GSN [P06396] | 3 | EBI-591818,EBI-351506 |
KMT2A [Q03164] | 4 | EBI-591818,EBI-591370 |
XAB2 [Q9HCS7] | 12 | EBI-591818,EBI-295232 |
Protein-protein interaction databases
BioGRIDi | 115714, 228 interactors |
CORUMi | Q9UNP9 |
DIPi | DIP-34757N |
IntActi | Q9UNP9, 63 interactors |
MINTi | Q9UNP9 |
STRINGi | 9606.ENSP00000361918 |
Miscellaneous databases
RNActi | Q9UNP9, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9UNP9 |
SMRi | Q9UNP9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UNP9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 84 | RRMPROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 143 – 299 | PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST | 157 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 60 – 63 | Poly-Ala | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0111, Eukaryota |
GeneTreei | ENSGT00940000158790 |
HOGENOMi | CLU_012062_27_0_1 |
InParanoidi | Q9UNP9 |
OMAi | KIVIYAC |
OrthoDBi | 1484092at2759 |
PhylomeDBi | Q9UNP9 |
TreeFami | TF313817 |
Family and domain databases
CDDi | cd12347, RRM_PPIE, 1 hit |
Gene3Di | 2.40.100.10, 1 hit 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR012677, Nucleotide-bd_a/b_plait_sf IPR016304, PPIE IPR034168, PPIE_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF001475, PPI_cyclophilin_E, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF50891, SSF50891, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit PS50102, RRM, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA
60 70 80 90 100
FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD
110 120 130 140 150
WLKKFSGKTL EENKEEEGSE PPKAETQEGE PIAKKARSNP QVYMDIKIGN
160 170 180 190 200
KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG
210 220 230 240 250
GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL
260 270 280 290 300
TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVIIADCGEY
V
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PEQ6 | E9PEQ6_HUMAN | Peptidyl-prolyl cis-trans isomerase | PPIE | 250 | Annotation score: | ||
E9PKY5 | E9PKY5_HUMAN | Peptidyl-prolyl cis-trans isomerase | PPIE | 213 | Annotation score: | ||
E9PK21 | E9PK21_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIE | 208 | Annotation score: | ||
E9PIB0 | E9PIB0_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIE | 71 | Annotation score: | ||
E9PMH0 | E9PMH0_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIE | 38 | Annotation score: | ||
H0YCV4 | H0YCV4_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIE | 37 | Annotation score: | ||
H0YE31 | H0YE31_HUMAN | Peptidyl-prolyl cis-trans isomerase... | PPIE | 36 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 151 | K → R in AAZ93379 (Ref. 3) Curated | 1 | |
Sequence conflicti | 207 | N → D in AAZ93379 (Ref. 3) Curated | 1 | |
Sequence conflicti | 287 | K → N in AAC00006 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046370 | 280 – 301 | AQGSK…CGEYV → VAPDTKASKARGSRKNKDGQ ERNWGKSQKVESHTI in isoform 3. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_005181 | 280 – 301 | AQGSK…CGEYV → KQEESAITSQPRSWKLT in isoform B. 2 PublicationsAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104012 mRNA Translation: AAD19906.1 AF104013 mRNA Translation: AAD19907.1 AF042385 mRNA Translation: AAC00006.1 AF042386 mRNA Translation: AAC00007.1 DQ160195 mRNA Translation: AAZ93379.1 AK313666 mRNA Translation: BAG36418.1 AL049824 Genomic DNA No translation available. AL033527 Genomic DNA No translation available. AL035404 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07255.1 BC004898 mRNA Translation: AAH04898.1 BC008451 mRNA Translation: AAH08451.1 BC107736 mRNA Translation: AAI07737.1 |
CCDSi | CCDS442.1 [Q9UNP9-2] CCDS443.1 [Q9UNP9-1] CCDS53299.1 [Q9UNP9-3] |
PIRi | S66681 |
RefSeqi | NP_001181936.1, NM_001195007.1 [Q9UNP9-3] NP_006103.1, NM_006112.3 [Q9UNP9-1] NP_982281.1, NM_203456.2 [Q9UNP9-2] XP_016855540.1, XM_017000051.1 [Q9UNP9-3] |
Genome annotation databases
Ensembli | ENST00000324379; ENSP00000312769; ENSG00000084072 [Q9UNP9-1] ENST00000356511; ENSP00000348904; ENSG00000084072 [Q9UNP9-2] ENST00000372830; ENSP00000361918; ENSG00000084072 [Q9UNP9-3] |
GeneIDi | 10450 |
KEGGi | hsa:10450 |
UCSCi | uc001cds.3, human [Q9UNP9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF104012 mRNA Translation: AAD19906.1 AF104013 mRNA Translation: AAD19907.1 AF042385 mRNA Translation: AAC00006.1 AF042386 mRNA Translation: AAC00007.1 DQ160195 mRNA Translation: AAZ93379.1 AK313666 mRNA Translation: BAG36418.1 AL049824 Genomic DNA No translation available. AL033527 Genomic DNA No translation available. AL035404 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07255.1 BC004898 mRNA Translation: AAH04898.1 BC008451 mRNA Translation: AAH08451.1 BC107736 mRNA Translation: AAI07737.1 |
CCDSi | CCDS442.1 [Q9UNP9-2] CCDS443.1 [Q9UNP9-1] CCDS53299.1 [Q9UNP9-3] |
PIRi | S66681 |
RefSeqi | NP_001181936.1, NM_001195007.1 [Q9UNP9-3] NP_006103.1, NM_006112.3 [Q9UNP9-1] NP_982281.1, NM_203456.2 [Q9UNP9-2] XP_016855540.1, XM_017000051.1 [Q9UNP9-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZMF | X-ray | 1.88 | A | 137-301 | [»] | |
2CQB | NMR | - | A | 1-89 | [»] | |
2KU7 | NMR | - | A | 2-82 | [»] | |
2KYX | NMR | - | A | 3-83 | [»] | |
2R99 | X-ray | 1.61 | A | 131-301 | [»] | |
3LPY | X-ray | 2.00 | A/B | 5-82 | [»] | |
3MDF | X-ray | 1.85 | A/B | 1-83 | [»] | |
3UCH | X-ray | 2.50 | A | 129-301 | [»] | |
5MQF | electron microscopy | 5.90 | o | 1-301 | [»] | |
5YZG | electron microscopy | 4.10 | 1 | 1-301 | [»] | |
5Z56 | electron microscopy | 5.10 | y | 1-301 | [»] | |
5Z57 | electron microscopy | 6.50 | y | 1-301 | [»] | |
6FF7 | electron microscopy | 4.50 | o | 1-301 | [»] | |
6ICZ | electron microscopy | 3.00 | y | 1-301 | [»] | |
6ID0 | electron microscopy | 2.90 | y | 1-301 | [»] | |
6ID1 | electron microscopy | 2.86 | y | 1-301 | [»] | |
7A5P | electron microscopy | 5.00 | o | 1-301 | [»] | |
BMRBi | Q9UNP9 | |||||
SMRi | Q9UNP9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115714, 228 interactors |
CORUMi | Q9UNP9 |
DIPi | DIP-34757N |
IntActi | Q9UNP9, 63 interactors |
MINTi | Q9UNP9 |
STRINGi | 9606.ENSP00000361918 |
PTM databases
iPTMneti | Q9UNP9 |
MetOSitei | Q9UNP9 |
PhosphoSitePlusi | Q9UNP9 |
Genetic variation databases
BioMutai | PPIE |
DMDMi | 13124097 |
Proteomic databases
EPDi | Q9UNP9 |
jPOSTi | Q9UNP9 |
MassIVEi | Q9UNP9 |
PeptideAtlasi | Q9UNP9 |
PRIDEi | Q9UNP9 |
ProteomicsDBi | 65110 85321 [Q9UNP9-1] 85322 [Q9UNP9-2] |
TopDownProteomicsi | Q9UNP9-1 [Q9UNP9-1] Q9UNP9-2 [Q9UNP9-2] |
Protocols and materials databases
Antibodypediai | 17854, 263 antibodies |
DNASUi | 10450 |
Genome annotation databases
Ensembli | ENST00000324379; ENSP00000312769; ENSG00000084072 [Q9UNP9-1] ENST00000356511; ENSP00000348904; ENSG00000084072 [Q9UNP9-2] ENST00000372830; ENSP00000361918; ENSG00000084072 [Q9UNP9-3] |
GeneIDi | 10450 |
KEGGi | hsa:10450 |
UCSCi | uc001cds.3, human [Q9UNP9-1] |
Organism-specific databases
CTDi | 10450 |
DisGeNETi | 10450 |
GeneCardsi | PPIE |
HGNCi | HGNC:9258, PPIE |
HPAi | ENSG00000084072, Low tissue specificity |
MIMi | 602435, gene |
neXtProti | NX_Q9UNP9 |
OpenTargetsi | ENSG00000084072 |
PharmGKBi | PA33583 |
VEuPathDBi | HostDB:ENSG00000084072.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0111, Eukaryota |
GeneTreei | ENSGT00940000158790 |
HOGENOMi | CLU_012062_27_0_1 |
InParanoidi | Q9UNP9 |
OMAi | KIVIYAC |
OrthoDBi | 1484092at2759 |
PhylomeDBi | Q9UNP9 |
TreeFami | TF313817 |
Enzyme and pathway databases
BRENDAi | 5.2.1.8, 2681 |
PathwayCommonsi | Q9UNP9 |
Reactomei | R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6798695, Neutrophil degranulation R-HSA-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 10450, 470 hits in 878 CRISPR screens |
ChiTaRSi | PPIE, human |
EvolutionaryTracei | Q9UNP9 |
GeneWikii | PPIE_(gene) |
GenomeRNAii | 10450 |
Pharosi | Q9UNP9, Tbio |
PROi | PR:Q9UNP9 |
RNActi | Q9UNP9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000084072, Expressed in right testis and 242 other tissues |
ExpressionAtlasi | Q9UNP9, baseline and differential |
Genevisiblei | Q9UNP9, HS |
Family and domain databases
CDDi | cd12347, RRM_PPIE, 1 hit |
Gene3Di | 2.40.100.10, 1 hit 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR012677, Nucleotide-bd_a/b_plait_sf IPR016304, PPIE IPR034168, PPIE_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF001475, PPI_cyclophilin_E, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF50891, SSF50891, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPIE_HUMAN | |
Accessioni | Q9UNP9Primary (citable) accession number: Q9UNP9 Secondary accession number(s): B2R971 Q9UIZ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM