UniProtKB - Q9UNI1 (CELA1_HUMAN)
Protein
Chymotrypsin-like elastase family member 1
Gene
CELA1
Organism
Homo sapiens (Human)
Status
Functioni
Acts upon elastin.
Caution
In spite of its original name 'Pancreatic elastase 1', CELA1 is not detected in the pancreas. Elastase activity described in the pancreas may be in fact due to CELA2A (PubMed:10620133).1 Publication
Catalytic activityi
- Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa. EC:3.4.21.36
Cofactori
Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 63 | Charge relay systemBy similarity | 1 | |
Metal bindingi | 77 | CalciumBy similarity | 1 | |
Metal bindingi | 82 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 87 | CalciumBy similarity | 1 | |
Active sitei | 111 | Charge relay systemBy similarity | 1 | |
Active sitei | 206 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- elastin catabolic process Source: Ensembl
- exocrine pancreas development Source: Ensembl
- inflammatory response Source: Ensembl
- multicellular organism growth Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- pancreas morphogenesis Source: Ensembl
- positive regulation of angiogenesis Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- post-embryonic development Source: Ensembl
- proteolysis Source: GO_Central
- regulation of cell differentiation Source: Ensembl
- regulation of cell population proliferation Source: Ensembl
- tissue remodeling Source: Ensembl
- Wnt signaling pathway Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.21.36, 2681 |
PathwayCommonsi | Q9UNI1 |
Protein family/group databases
MEROPSi | S01.153 |
Names & Taxonomyi
Protein namesi | Recommended name: Chymotrypsin-like elastase family member 1 (EC:3.4.21.36)Alternative name(s): Elastase-1 Pancreatic elastase 1 |
Gene namesi | Name:CELA1 Synonyms:ELA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000139610.1 |
HGNCi | HGNC:3308, CELA1 |
MIMi | 130120, gene |
neXtProti | NX_Q9UNI1 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 1990 |
OpenTargetsi | ENSG00000139610 |
PharmGKBi | PA27734 |
Miscellaneous databases
Pharosi | Q9UNI1, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3000 |
DrugBanki | DB07955, (2-BROMOETHYL)(2-'FORMYL-4'-AMINOPHENYL) ACETATE DB08007, (2R)-3-{[(BENZYLAMINO)CARBONYL]AMINO}-2-HYDROXYPROPANOIC ACID DB08640, (2S,3S)-3-FORMYL-2-({[(4-METHYLPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID DB08641, (2S,3S)-3-FORMYL-2-({[(4-NITROPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID DB06951, (3R)-3-ethyl-N-[(4-methylphenyl)sulfonyl]-L-aspartic acid DB07433, (TERT-BUTYLOXYCARBONYL)-ALANYL-AMINO ETHYL-FORMAMIDE DB03202, 2-[5-Methanesulfonylamino-2-(4-Aminophenyl)-6-Oxo-1,6-Dihydro-1-Pyrimidinyl]-N-(3,3,3-Trifluoro-1-Isopropyl-2-Oxopropyl)Acetamide DB08614, 3-[[(METHYLAMINO)SULFONYL]AMINO]-2-OXO-6-PHENYL-N-[3,3,3-TRIFLUORO-1-(1-METHYLETHYL)-2-OXOPHENYL]-1(2H)-PYRIDINE ACETAMIDE DB07956, [1-(3-CHLORO-2-FORMYL-PHENYLCARBAMOYL)-2-METHYL-PROPYL]-CARBAMIC ACID TERT-BUTYL ESTER DB02114, Cumidine DB03925, Freselestat DB02341, Mdl 101,146 DB07957, METHYL(2-ACETOXY-2-(2-CARBOXY-4-AMINO-PHENYL))ACETATE DB01844, N,N-dimethylformamide DB03890, N-[2-(1-Formyl-2-Methyl-Propyl)-1-(4-Piperidin-1-Yl-but-2-Enoyl)-Pyrrolidin-3-Yl]-Methanesulfonamide DB03702, N-{4-[(Carboxymethyl)carbamoyl]benzoyl}-L-valyl-N-[(3S)-1,1,1-trifluoro-4-methyl-2-oxo-3-pentanyl]-L-prolinamide DB03757, N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-alanyl-L-alaninamide |
DrugCentrali | Q9UNI1 |
GuidetoPHARMACOLOGYi | 2338 |
Polymorphism and mutation databases
BioMutai | CELA1 |
DMDMi | 62298049 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 8 | By similarity | 8 | |
PropeptideiPRO_0000027677 | 9 – 18 | Activation peptide | 10 | |
ChainiPRO_0000027678 | 19 – 258 | Chymotrypsin-like elastase family member 1Add BLAST | 240 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 48 ↔ 64 | PROSITE-ProRule annotation | ||
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 145 ↔ 212 | PROSITE-ProRule annotation | ||
Disulfide bondi | 176 ↔ 192 | PROSITE-ProRule annotation | ||
Disulfide bondi | 202 ↔ 232 | PROSITE-ProRule annotation | ||
Glycosylationi | 233 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
MassIVEi | Q9UNI1 |
PaxDbi | Q9UNI1 |
PeptideAtlasi | Q9UNI1 |
PRIDEi | Q9UNI1 |
ProteomicsDBi | 85297 |
PTM databases
GlyGeni | Q9UNI1, 2 sites |
Expressioni
Tissue specificityi
Basal layers of epidermis (at protein level). Not expressed in the pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000139610, Expressed in pancreas and 37 other tissues |
Genevisiblei | Q9UNI1, HS |
Organism-specific databases
HPAi | ENSG00000139610, Group enriched (adrenal gland, blood, pancreas) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 108305, 18 interactors |
IntActi | Q9UNI1, 16 interactors |
STRINGi | 9606.ENSP00000293636 |
Chemistry databases
BindingDBi | Q9UNI1 |
Miscellaneous databases
RNActi | Q9UNI1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 256 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 238 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01000000214372 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | Q9UNI1 |
OMAi | HSGCQGD |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9UNI1 |
TreeFami | TF330455 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9UNI1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLVLYGHSTQ DLPETNARVV GGTEAGRNSW PSQISLQYRS GGSRYHTCGG
60 70 80 90 100
TLIRQNWVMT AAHCVDYQKT FRVVAGDHNL SQNDGTEQYV SVQKIVVHPY
110 120 130 140 150
WNSDNVAAGY DIALLRLAQS VTLNSYVQLG VLPQEGAILA NNSPCYITGW
160 170 180 190 200
GKTKTNGQLA QTLQQAYLPS VDYAICSSSS YWGSTVKNTM VCAGGDGVRS
210 220 230 240 250
GCQGDSGGPL HCLVNGKYSV HGVTSFVSSR GCNVSRKPTV FTQVSAYISW
INNVIASN
Sequence cautioni
The sequence AAV88109 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 | V → L in AAD28441 (PubMed:10620133).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033645 | 10 | Q → H. Corresponds to variant dbSNP:rs17860287Ensembl. | 1 | |
Natural variantiVAR_033646 | 44 | R → W. Corresponds to variant dbSNP:rs17860299Ensembl. | 1 | |
Natural variantiVAR_033647 | 59 | M → V. Corresponds to variant dbSNP:rs17860300Ensembl. | 1 | |
Natural variantiVAR_036295 | 76 | G → A in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_033648 | 243 | Q → R. Corresponds to variant dbSNP:rs17860364Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF120493 mRNA Translation: AAD28441.1 AC046135 Genomic DNA No translation available. BC069454 mRNA Translation: AAH69454.1 BC075091 mRNA Translation: AAH75091.2 AY740424 Genomic DNA Translation: AAV88109.1 Sequence problems. |
CCDSi | CCDS8812.1 |
RefSeqi | NP_001962.3, NM_001971.5 |
Genome annotation databases
Ensembli | ENST00000293636; ENSP00000293636; ENSG00000139610 |
GeneIDi | 1990 |
KEGGi | hsa:1990 |
UCSCi | uc001ryi.1, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Wikipedia Elastase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF120493 mRNA Translation: AAD28441.1 AC046135 Genomic DNA No translation available. BC069454 mRNA Translation: AAH69454.1 BC075091 mRNA Translation: AAH75091.2 AY740424 Genomic DNA Translation: AAV88109.1 Sequence problems. |
CCDSi | CCDS8812.1 |
RefSeqi | NP_001962.3, NM_001971.5 |
3D structure databases
SMRi | Q9UNI1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 108305, 18 interactors |
IntActi | Q9UNI1, 16 interactors |
STRINGi | 9606.ENSP00000293636 |
Chemistry databases
BindingDBi | Q9UNI1 |
ChEMBLi | CHEMBL3000 |
DrugBanki | DB07955, (2-BROMOETHYL)(2-'FORMYL-4'-AMINOPHENYL) ACETATE DB08007, (2R)-3-{[(BENZYLAMINO)CARBONYL]AMINO}-2-HYDROXYPROPANOIC ACID DB08640, (2S,3S)-3-FORMYL-2-({[(4-METHYLPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID DB08641, (2S,3S)-3-FORMYL-2-({[(4-NITROPHENYL)SULFONYL]AMINO}METHYL)PENTANOIC ACID DB06951, (3R)-3-ethyl-N-[(4-methylphenyl)sulfonyl]-L-aspartic acid DB07433, (TERT-BUTYLOXYCARBONYL)-ALANYL-AMINO ETHYL-FORMAMIDE DB03202, 2-[5-Methanesulfonylamino-2-(4-Aminophenyl)-6-Oxo-1,6-Dihydro-1-Pyrimidinyl]-N-(3,3,3-Trifluoro-1-Isopropyl-2-Oxopropyl)Acetamide DB08614, 3-[[(METHYLAMINO)SULFONYL]AMINO]-2-OXO-6-PHENYL-N-[3,3,3-TRIFLUORO-1-(1-METHYLETHYL)-2-OXOPHENYL]-1(2H)-PYRIDINE ACETAMIDE DB07956, [1-(3-CHLORO-2-FORMYL-PHENYLCARBAMOYL)-2-METHYL-PROPYL]-CARBAMIC ACID TERT-BUTYL ESTER DB02114, Cumidine DB03925, Freselestat DB02341, Mdl 101,146 DB07957, METHYL(2-ACETOXY-2-(2-CARBOXY-4-AMINO-PHENYL))ACETATE DB01844, N,N-dimethylformamide DB03890, N-[2-(1-Formyl-2-Methyl-Propyl)-1-(4-Piperidin-1-Yl-but-2-Enoyl)-Pyrrolidin-3-Yl]-Methanesulfonamide DB03702, N-{4-[(Carboxymethyl)carbamoyl]benzoyl}-L-valyl-N-[(3S)-1,1,1-trifluoro-4-methyl-2-oxo-3-pentanyl]-L-prolinamide DB03757, N-{[(2-Methyl-2-propanyl)oxy]carbonyl}-L-alanyl-L-alaninamide |
DrugCentrali | Q9UNI1 |
GuidetoPHARMACOLOGYi | 2338 |
Protein family/group databases
MEROPSi | S01.153 |
PTM databases
GlyGeni | Q9UNI1, 2 sites |
Polymorphism and mutation databases
BioMutai | CELA1 |
DMDMi | 62298049 |
Proteomic databases
MassIVEi | Q9UNI1 |
PaxDbi | Q9UNI1 |
PeptideAtlasi | Q9UNI1 |
PRIDEi | Q9UNI1 |
ProteomicsDBi | 85297 |
Protocols and materials databases
Antibodypediai | 14377, 153 antibodies |
Genome annotation databases
Ensembli | ENST00000293636; ENSP00000293636; ENSG00000139610 |
GeneIDi | 1990 |
KEGGi | hsa:1990 |
UCSCi | uc001ryi.1, human |
Organism-specific databases
CTDi | 1990 |
DisGeNETi | 1990 |
EuPathDBi | HostDB:ENSG00000139610.1 |
GeneCardsi | CELA1 |
HGNCi | HGNC:3308, CELA1 |
HPAi | ENSG00000139610, Group enriched (adrenal gland, blood, pancreas) |
MIMi | 130120, gene |
neXtProti | NX_Q9UNI1 |
OpenTargetsi | ENSG00000139610 |
PharmGKBi | PA27734 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT01000000214372 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | Q9UNI1 |
OMAi | HSGCQGD |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9UNI1 |
TreeFami | TF330455 |
Enzyme and pathway databases
BRENDAi | 3.4.21.36, 2681 |
PathwayCommonsi | Q9UNI1 |
Miscellaneous databases
BioGRID-ORCSi | 1990, 7 hits in 844 CRISPR screens |
ChiTaRSi | CELA1, human |
GeneWikii | CELA1 |
GenomeRNAii | 1990 |
Pharosi | Q9UNI1, Tchem |
PROi | PR:Q9UNI1 |
RNActi | Q9UNI1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000139610, Expressed in pancreas and 37 other tissues |
Genevisiblei | Q9UNI1, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CELA1_HUMAN | |
Accessioni | Q9UNI1Primary (citable) accession number: Q9UNI1 Secondary accession number(s): Q5MLF0, Q6DJT0, Q6ISM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | March 29, 2005 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Peptidase families
Classification of peptidase families and list of entries