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Protein

DNA polymerase iota

Gene

POLI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.7 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Magnesium1
Binding sitei64dNTP1
Binding sitei96dNTP1
Metal bindingi151Magnesium1
Active sitei152Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: ProtInc
  • DNA replication Source: UniProtKB-KW
  • error-prone translesion synthesis Source: Reactome
  • translesion synthesis Source: Reactome

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandMagnesium, Metal-binding, Schiff base

Enzyme and pathway databases

BRENDAi2.7.7.7 2681
ReactomeiR-HSA-5656121 Translesion synthesis by POLI
R-HSA-5656169 Termination of translesion DNA synthesis

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase iota (EC:2.7.7.7)
Alternative name(s):
Eta2
RAD30 homolog B
Gene namesi
Name:POLI
Synonyms:RAD30B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000101751.10
HGNCiHGNC:9182 POLI
MIMi605252 gene
neXtProtiNX_Q9UNA4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11201
OpenTargetsiENSG00000101751
PharmGKBiPA33502

Chemistry databases

ChEMBLiCHEMBL5391

Polymorphism and mutation databases

BioMutaiPOLI
DMDMi327478565

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739881 – 740DNA polymerase iotaAdd BLAST740

Proteomic databases

EPDiQ9UNA4
MaxQBiQ9UNA4
PaxDbiQ9UNA4
PeptideAtlasiQ9UNA4
PRIDEiQ9UNA4
ProteomicsDBi85277

PTM databases

iPTMnetiQ9UNA4
PhosphoSitePlusiQ9UNA4

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in testis.2 Publications

Gene expression databases

BgeeiENSG00000101751 Expressed in 214 organ(s), highest expression level in kidney
CleanExiHS_POLI
ExpressionAtlasiQ9UNA4 baseline and differential
GenevisibleiQ9UNA4 HS

Organism-specific databases

HPAiHPA012000
HPA064696

Interactioni

Subunit structurei

Interacts with REV1 (By similarity). Interacts with POLH.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi116370, 18 interactors
IntActiQ9UNA4, 18 interactors
MINTiQ9UNA4
STRINGi9606.ENSP00000462664

Chemistry databases

BindingDBiQ9UNA4

Structurei

Secondary structure

1740
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UNA4
SMRiQ9UNA4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UNA4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 268UmuCPROSITE-ProRule annotationAdd BLAST214

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 332DNA binding8
Regioni368 – 386DNA bindingAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi589 – 617Ser-richAdd BLAST29

Domaini

The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

eggNOGiKOG2095 Eukaryota
COG0389 LUCA
GeneTreeiENSGT00530000062942
HOGENOMiHOG000234325
HOVERGENiHBG053634
InParanoidiQ9UNA4
KOiK03510
OMAiSFKMKDT
OrthoDBiEOG091G033F
PhylomeDBiQ9UNA4
TreeFamiTF324222

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

Q9UNA4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA
60 70 80 90 100
SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV
110 120 130 140 150
KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF
160 170 180 190 200
DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV
210 220 230 240 250
GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES
260 270 280 290 300
CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI
310 320 330 340 350
SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL
360 370 380 390 400
ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG
410 420 430 440 450
NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID
460 470 480 490 500
YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR
510 520 530 540 550
ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE
560 570 580 590 600
KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC
610 620 630 640 650
EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN
660 670 680 690 700
PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE
710 720 730 740
KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK
Length:740
Mass (Da):83,006
Last modified:April 5, 2011 - v3
Checksum:iC1A5BF0894E91FDF
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KSW2J3KSW2_HUMAN
DNA polymerase iota
POLI
637Annotation score:
X6R2I3X6R2I3_HUMAN
DNA polymerase iota
POLI
678Annotation score:
J3KTN3J3KTN3_HUMAN
DNA polymerase iota
POLI
222Annotation score:
J3KQ09J3KQ09_HUMAN
DNA polymerase iota
POLI
661Annotation score:
J3KTM8J3KTM8_HUMAN
DNA polymerase iota
POLI
142Annotation score:
J3KRS8J3KRS8_HUMAN
DNA polymerase iota
POLI
85Annotation score:
J3KRG0J3KRG0_HUMAN
DNA polymerase iota
POLI
145Annotation score:
J3QR36J3QR36_HUMAN
DNA polymerase iota
POLI
195Annotation score:
J3QQZ8J3QQZ8_HUMAN
DNA polymerase iota
POLI
52Annotation score:

Sequence cautioni

The sequence AAD50381 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF63383 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH32662 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAM11872 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB66605 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15Missing in AAD50381 (PubMed:10458907).Curated1
Sequence conflicti15Missing in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti15Missing in AAF63383 (Ref. 6) Curated1
Sequence conflicti337S → T in CAB66605 (PubMed:11230166).Curated1
Sequence conflicti433V → A in AAF63383 (Ref. 6) Curated1
Sequence conflicti514D → G in AAF63383 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02123996R → G1 PublicationCorresponds to variant dbSNP:rs3218778Ensembl.1
Natural variantiVAR_021240261I → M1 PublicationCorresponds to variant dbSNP:rs3218784Ensembl.1
Natural variantiVAR_021241276E → K1 PublicationCorresponds to variant dbSNP:rs3218783Ensembl.1
Natural variantiVAR_021242474H → R1 PublicationCorresponds to variant dbSNP:rs3730823Ensembl.1
Natural variantiVAR_021243532F → S2 PublicationsCorresponds to variant dbSNP:rs3218786Ensembl.1
Natural variantiVAR_021244560C → R1 PublicationCorresponds to variant dbSNP:rs3218787Ensembl.1
Natural variantiVAR_021245731A → T2 PublicationsCorresponds to variant dbSNP:rs8305Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY094607 Genomic DNA Translation: AAM11872.1 Sequence problems.
AC093462 Genomic DNA No translation available.
AF140501 mRNA Translation: AAD50381.1 Different initiation.
AL136670 mRNA Translation: CAB66605.1 Different initiation.
BC032662 mRNA Translation: AAH32662.1 Different initiation.
AF245438 mRNA Translation: AAF63383.1 Different initiation.
CCDSiCCDS11954.2
RefSeqiNP_009126.2, NM_007195.2
UniGeneiHs.438533

Genome annotation databases

EnsembliENST00000579534; ENSP00000462664; ENSG00000101751
GeneIDi11201
KEGGihsa:11201
UCSCiuc002lfj.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY094607 Genomic DNA Translation: AAM11872.1 Sequence problems.
AC093462 Genomic DNA No translation available.
AF140501 mRNA Translation: AAD50381.1 Different initiation.
AL136670 mRNA Translation: CAB66605.1 Different initiation.
BC032662 mRNA Translation: AAH32662.1 Different initiation.
AF245438 mRNA Translation: AAF63383.1 Different initiation.
CCDSiCCDS11954.2
RefSeqiNP_009126.2, NM_007195.2
UniGeneiHs.438533

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3NX-ray2.30A/B52-439[»]
1ZETX-ray2.30A52-439[»]
2ALZX-ray2.50A50-439[»]
2DPIX-ray2.30A26-445[»]
2DPJX-ray2.30A26-445[»]
2FLLX-ray2.60A26-445[»]
2FLNX-ray2.50A26-445[»]
2FLPX-ray2.40A26-445[»]
2KHUNMR-A697-740[»]
2KHWNMR-A697-740[»]
2KTFNMR-B704-730[»]
2L0FNMR-B699-740[»]
2L0GNMR-A704-730[»]
2MBBNMR-A516-555[»]
3EPGX-ray2.50A26-445[»]
3EPIX-ray2.90A26-445[»]
3G6VX-ray2.20A26-445[»]
3G6XX-ray2.08A26-445[»]
3G6YX-ray2.10A26-445[»]
3GV5X-ray2.00B/D26-445[»]
3GV7X-ray2.20B26-445[»]
3GV8X-ray2.00B26-445[»]
3H40X-ray2.30A51-439[»]
3H4BX-ray2.85A50-439[»]
3H4DX-ray2.20A50-439[»]
3NGDX-ray2.80A26-445[»]
3OSNX-ray1.90A26-445[»]
3Q8PX-ray1.95B26-445[»]
3Q8QX-ray2.03B26-445[»]
3Q8RX-ray2.45B26-445[»]
3Q8SX-ray2.09B26-445[»]
4EBCX-ray2.90A26-445[»]
4EBDX-ray2.57A26-445[»]
4EBEX-ray2.10A26-445[»]
4EYHX-ray2.90B26-445[»]
4EYIX-ray2.90B26-445[»]
4FS1X-ray2.50A26-445[»]
4FS2X-ray2.05A26-445[»]
5KT2X-ray2.49A26-445[»]
5KT3X-ray2.64A26-445[»]
5KT4X-ray2.78A1-445[»]
5KT5X-ray2.80A1-445[»]
5KT6X-ray3.54A1-445[»]
5KT7X-ray3.15A1-445[»]
5ULWX-ray2.62A26-445[»]
5ULXX-ray1.96A26-445[»]
ProteinModelPortaliQ9UNA4
SMRiQ9UNA4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116370, 18 interactors
IntActiQ9UNA4, 18 interactors
MINTiQ9UNA4
STRINGi9606.ENSP00000462664

Chemistry databases

BindingDBiQ9UNA4
ChEMBLiCHEMBL5391

PTM databases

iPTMnetiQ9UNA4
PhosphoSitePlusiQ9UNA4

Polymorphism and mutation databases

BioMutaiPOLI
DMDMi327478565

Proteomic databases

EPDiQ9UNA4
MaxQBiQ9UNA4
PaxDbiQ9UNA4
PeptideAtlasiQ9UNA4
PRIDEiQ9UNA4
ProteomicsDBi85277

Protocols and materials databases

DNASUi11201
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000579534; ENSP00000462664; ENSG00000101751
GeneIDi11201
KEGGihsa:11201
UCSCiuc002lfj.5 human

Organism-specific databases

CTDi11201
DisGeNETi11201
EuPathDBiHostDB:ENSG00000101751.10
GeneCardsiPOLI
H-InvDBiHIX0014457
HGNCiHGNC:9182 POLI
HPAiHPA012000
HPA064696
MIMi605252 gene
neXtProtiNX_Q9UNA4
OpenTargetsiENSG00000101751
PharmGKBiPA33502
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2095 Eukaryota
COG0389 LUCA
GeneTreeiENSGT00530000062942
HOGENOMiHOG000234325
HOVERGENiHBG053634
InParanoidiQ9UNA4
KOiK03510
OMAiSFKMKDT
OrthoDBiEOG091G033F
PhylomeDBiQ9UNA4
TreeFamiTF324222

Enzyme and pathway databases

BRENDAi2.7.7.7 2681
ReactomeiR-HSA-5656121 Translesion synthesis by POLI
R-HSA-5656169 Termination of translesion DNA synthesis

Miscellaneous databases

ChiTaRSiPOLI human
EvolutionaryTraceiQ9UNA4
GeneWikiiPOLI
GenomeRNAii11201
PROiPR:Q9UNA4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101751 Expressed in 214 organ(s), highest expression level in kidney
CleanExiHS_POLI
ExpressionAtlasiQ9UNA4 baseline and differential
GenevisibleiQ9UNA4 HS

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPOLI_HUMAN
AccessioniPrimary (citable) accession number: Q9UNA4
Secondary accession number(s): Q8N590, Q9H0S1, Q9NYH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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