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Protein

Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

Gene

DAPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.1 Publication

GO - Molecular functioni

  • PDZ domain binding Source: GO_Central
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  • phosphatidylinositol-3,4-bisphosphate binding Source: UniProtKB
  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • protein dephosphorylation Source: UniProtKB
  • signal transduction Source: ProtInc

Enzyme and pathway databases

ReactomeiR-HSA-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
SignaLinkiQ9UN19
SIGNORiQ9UN19

Names & Taxonomyi

Protein namesi
Recommended name:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Short name:
hDAPP1
Alternative name(s):
B lymphocyte adapter protein Bam32
B-cell adapter molecule of 32 kDa
Gene namesi
Name:DAPP1
Synonyms:BAM32
ORF Names:HSPC066
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000070190.12
HGNCiHGNC:16500 DAPP1
MIMi605768 gene
neXtProtiNX_Q9UN19

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61R → K: No change in BCR-induced NFAT activation. 1 Publication1
Mutagenesisi173K → L: No interaction with 3-phosphoinositides. 1 Publication1
Mutagenesisi184R → C: No membrane association. 1 Publication1
Mutagenesisi197K → E: No membrane association. 1 Publication1
Mutagenesisi250W → L: No interaction with 3-phosphoinositides. 1 Publication1

Organism-specific databases

DisGeNETi27071
OpenTargetsiENSG00000070190
PharmGKBiPA27145

Polymorphism and mutation databases

BioMutaiDAPP1
DMDMi51317293

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797851 – 280Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositideAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphotyrosineBy similarity1
Modified residuei141PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UN19
PaxDbiQ9UN19
PeptideAtlasiQ9UN19
PRIDEiQ9UN19
ProteomicsDBi85238
85239 [Q9UN19-2]

PTM databases

iPTMnetiQ9UN19
PhosphoSitePlusiQ9UN19

Expressioni

Tissue specificityi

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.1 Publication

Inductioni

Upon B-cell activation.

Gene expression databases

BgeeiENSG00000070190 Expressed in 209 organ(s), highest expression level in amniotic fluid
CleanExiHS_DAPP1
ExpressionAtlasiQ9UN19 baseline and differential
GenevisibleiQ9UN19 HS

Organism-specific databases

HPAiCAB033822
HPA046074

Interactioni

Subunit structurei

Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117981, 16 interactors
IntActiQ9UN19, 20 interactors
MINTiQ9UN19
STRINGi9606.ENSP00000423602

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UN19
SMRiQ9UN19
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UN19

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 129SH2PROSITE-ProRule annotationAdd BLAST95
Domaini164 – 259PHPROSITE-ProRule annotationAdd BLAST96

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IF6C Eukaryota
ENOG410XSW5 LUCA
GeneTreeiENSGT00910000144274
HOGENOMiHOG000112147
HOVERGENiHBG051297
InParanoidiQ9UN19
KOiK12229
OMAiKVNCFCL
OrthoDBiEOG091G0HCX
PhylomeDBiQ9UN19
TreeFamiTF105418

Family and domain databases

CDDicd10355 SH2_DAPP1_BAM32_like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035843 DAPP1_SH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50001 SH2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UN19-1) [UniParc]FASTAAdd to basket
Also known as: hBam1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGRAELLEGK MSTQDPSDLW SRSDGEAELL QDLGWYHGNL TRHAAEALLL
60 70 80 90 100
SNGCDGSYLL RDSNETTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEFS
110 120 130 140 150
SLKDFVKHFA NQPLIGSETG TLMVLKHPYP RKVEEPSIYE SVRVHTAMQT
160 170 180 190 200
GRTEDDLVPT APSLGTKEGY LTKQGGLVKT WKTRWFTLHR NELKYFKDQM
210 220 230 240 250
SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC AKTGVEADEW
260 270 280
IKILRWKLSQ IRKQLNQGEG TIRSRSFIFK
Length:280
Mass (Da):32,194
Last modified:May 1, 2000 - v1
Checksum:i4A7F686B55146241
GO
Isoform 2 (identifier: Q9UN19-2) [UniParc]FASTAAdd to basket
Also known as: hBam2

The sequence of this isoform differs from the canonical sequence as follows:
     259-280: SQIRKQLNQGEGTIRSRSFIFK → VKDKSCILSALCISPEEKTDHK

Show »
Length:280
Mass (Da):32,044
Checksum:i643DF0A3C4FB32DC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KNB3J3KNB3_HUMAN
Dual adapter for phosphotyrosine an...
DAPP1 hCG_38736
263Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216 – 229VQFDY…VNCFC → MICNILCSFFCPIS (PubMed:17974005).CuratedAdd BLAST14

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010699259 – 280SQIRK…SFIFK → VKDKSCILSALCISPEEKTD HK in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163254 mRNA Translation: AAD49697.1
AF161551 mRNA Translation: AAF29038.1
AF186022 mRNA Translation: AAF14578.1
AF178987 mRNA Translation: AAF44351.1
BC012924 mRNA Translation: AAH12924.1
AL713793 mRNA Translation: CAD28547.1
CCDSiCCDS47112.1 [Q9UN19-1]
RefSeqiNP_001293080.1, NM_001306151.1
NP_055210.2, NM_014395.2 [Q9UN19-1]
UniGeneiHs.436271
Hs.708484

Genome annotation databases

EnsembliENST00000512369; ENSP00000423602; ENSG00000070190 [Q9UN19-1]
GeneIDi27071
KEGGihsa:27071
UCSCiuc003hvf.5 human [Q9UN19-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163254 mRNA Translation: AAD49697.1
AF161551 mRNA Translation: AAF29038.1
AF186022 mRNA Translation: AAF14578.1
AF178987 mRNA Translation: AAF44351.1
BC012924 mRNA Translation: AAH12924.1
AL713793 mRNA Translation: CAD28547.1
CCDSiCCDS47112.1 [Q9UN19-1]
RefSeqiNP_001293080.1, NM_001306151.1
NP_055210.2, NM_014395.2 [Q9UN19-1]
UniGeneiHs.436271
Hs.708484

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FAOX-ray1.80A148-273[»]
1FB8X-ray2.40A148-273[»]
ProteinModelPortaliQ9UN19
SMRiQ9UN19
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117981, 16 interactors
IntActiQ9UN19, 20 interactors
MINTiQ9UN19
STRINGi9606.ENSP00000423602

PTM databases

iPTMnetiQ9UN19
PhosphoSitePlusiQ9UN19

Polymorphism and mutation databases

BioMutaiDAPP1
DMDMi51317293

Proteomic databases

EPDiQ9UN19
PaxDbiQ9UN19
PeptideAtlasiQ9UN19
PRIDEiQ9UN19
ProteomicsDBi85238
85239 [Q9UN19-2]

Protocols and materials databases

DNASUi27071
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000512369; ENSP00000423602; ENSG00000070190 [Q9UN19-1]
GeneIDi27071
KEGGihsa:27071
UCSCiuc003hvf.5 human [Q9UN19-1]

Organism-specific databases

CTDi27071
DisGeNETi27071
EuPathDBiHostDB:ENSG00000070190.12
GeneCardsiDAPP1
H-InvDBiHIX0004401
HGNCiHGNC:16500 DAPP1
HPAiCAB033822
HPA046074
MIMi605768 gene
neXtProtiNX_Q9UN19
OpenTargetsiENSG00000070190
PharmGKBiPA27145
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF6C Eukaryota
ENOG410XSW5 LUCA
GeneTreeiENSGT00910000144274
HOGENOMiHOG000112147
HOVERGENiHBG051297
InParanoidiQ9UN19
KOiK12229
OMAiKVNCFCL
OrthoDBiEOG091G0HCX
PhylomeDBiQ9UN19
TreeFamiTF105418

Enzyme and pathway databases

ReactomeiR-HSA-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
SignaLinkiQ9UN19
SIGNORiQ9UN19

Miscellaneous databases

EvolutionaryTraceiQ9UN19
GeneWikiiDAPP1
GenomeRNAii27071
PROiPR:Q9UN19
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000070190 Expressed in 209 organ(s), highest expression level in amniotic fluid
CleanExiHS_DAPP1
ExpressionAtlasiQ9UN19 baseline and differential
GenevisibleiQ9UN19 HS

Family and domain databases

CDDicd10355 SH2_DAPP1_BAM32_like, 1 hit
Gene3Di2.30.29.30, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035843 DAPP1_SH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000980 SH2
IPR036860 SH2_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDAPP1_HUMAN
AccessioniPrimary (citable) accession number: Q9UN19
Secondary accession number(s): Q8TCK5, Q9UHF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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