UniProtKB - Q9UMR2 (DD19B_HUMAN)
Protein
ATP-dependent RNA helicase DDX19B
Gene
DDX19B
Organism
Homo sapiens (Human)
Status
Functioni
ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971).1 Publication
Catalytic activityi
- EC:3.6.4.131 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 119 | ATP | 1 | |
Binding sitei | 429 | ATP | 1 | |
Binding sitei | 432 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 138 – 145 | ATP | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- helicase activity Source: ProtInc
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
GO - Biological processi
- mRNA export from nucleus Source: ProtInc
- poly(A)+ mRNA export from nucleus Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.4.13, 2681 |
PathwayCommonsi | Q9UMR2 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DDX19B (EC:3.6.4.131 Publication)Alternative name(s): DEAD box RNA helicase DEAD5 DEAD box protein 19B |
Gene namesi | Name:DDX19B Synonyms:DBP5, DDX19, TDBP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000157349.16 |
HGNCi | HGNC:2742, DDX19B |
MIMi | 605812, gene |
neXtProti | NX_Q9UMR2 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
Other locations
- Cytoplasm 1 Publication
Note: Associates with the nuclear pore complex cytoplasmic fibrils.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nuclear envelope Source: UniProtKB
- nuclear pore Source: ProtInc
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: ProtInc
- cytoplasmic stress granule Source: GO_Central
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 223 | D → R: Impairs interaction with NUP214 and RNA. 1 Publication | 1 | |
Mutagenesisi | 243 | E → Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 258 | I → A: Impairs interaction with NUP214. 1 Publication | 1 | |
Mutagenesisi | 259 | R → D: Impairs interaction with NUP214. 1 Publication | 1 | |
Mutagenesisi | 262 | R → A: Impairs interaction with NUP214. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11269 |
OpenTargetsi | ENSG00000157349 |
PharmGKBi | PA27208 |
Miscellaneous databases
Pharosi | Q9UMR2, Tbio |
Polymorphism and mutation databases
BioMutai | DDX19B |
DMDMi | 10719979 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000055022 | 2 – 479 | ATP-dependent RNA helicase DDX19BAdd BLAST | 478 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9UMR2 |
jPOSTi | Q9UMR2 |
MassIVEi | Q9UMR2 |
PaxDbi | Q9UMR2 |
PeptideAtlasi | Q9UMR2 |
PRIDEi | Q9UMR2 |
ProteomicsDBi | 16960 85194 [Q9UMR2-1] 85195 [Q9UMR2-2] 85196 [Q9UMR2-3] |
PTM databases
iPTMneti | Q9UMR2 |
PhosphoSitePlusi | Q9UMR2 |
Expressioni
Gene expression databases
Bgeei | ENSG00000157349, Expressed in testis and 148 other tissues |
ExpressionAtlasi | Q9UMR2, baseline and differential |
Genevisiblei | Q9UMR2, HS |
Organism-specific databases
HPAi | ENSG00000157349, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9UMR2
With | #Exp. | IntAct |
---|---|---|
CTIF - isoform 2 [O43310-2] | 3 | EBI-719232,EBI-12180013 |
MIF4GD [A9UHW6] | 11 | EBI-719232,EBI-373498 |
MIF4GD - isoform 2 [A9UHW6-2] | 6 | EBI-719232,EBI-9118295 |
TERF1 [P54274] | 2 | EBI-719232,EBI-710997 |
Isoform 1 [Q9UMR2-1]
With | #Exp. | IntAct |
---|---|---|
NUP214 - isoform 1 [P35658-1] | 9 | EBI-5773937,EBI-15757000 |
Protein-protein interaction databases
BioGRIDi | 116426, 158 interactors |
DIPi | DIP-48486N |
IntActi | Q9UMR2, 94 interactors |
MINTi | Q9UMR2 |
STRINGi | 9606.ENSP00000288071 |
Miscellaneous databases
RNActi | Q9UMR2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UMR2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UMR2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 125 – 295 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 171 | |
Domaini | 306 – 474 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 169 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 300 | N-terminal lobeAdd BLAST | 299 | |
Regioni | 55 – 68 | N-terminal helixAdd BLAST | 14 | |
Regioni | 301 – 479 | C-terminal lobeAdd BLAST | 179 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 92 – 120 | Q motifAdd BLAST | 29 | |
Motifi | 242 – 245 | DEAD box | 4 |
Domaini
The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0332, Eukaryota |
GeneTreei | ENSGT00940000154417 |
HOGENOMi | CLU_003041_1_0_1 |
InParanoidi | Q9UMR2 |
OrthoDBi | 608788at2759 |
PhylomeDBi | Q9UMR2 |
TreeFami | TF314957 |
Family and domain databases
DisProti | DP01560 |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9UMR2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATDSWALAV DEQEAAAESL SNLHLKEEKI KPDTNGAVVK TNANAEKTDE
60 70 80 90 100
EEKEDRAAQS LLNKLIRSNL VDNTNQVEVL QRDPNSPLYS VKSFEELRLK
110 120 130 140 150
PQLLQGVYAM GFNRPSKIQE NALPLMLAEP PQNLIAQSQS GTGKTAAFVL
160 170 180 190 200
AMLSQVEPAN KYPQCLCLSP TYELALQTGK VIEQMGKFYP ELKLAYAVRG
210 220 230 240 250
NKLERGQKIS EQIVIGTPGT VLDWCSKLKF IDPKKIKVFV LDEADVMIAT
260 270 280 290 300
QGHQDQSIRI QRMLPRNCQM LLFSATFEDS VWKFAQKVVP DPNVIKLKRE
310 320 330 340 350
EETLDTIKQY YVLCSSRDEK FQALCNLYGA ITIAQAMIFC HTRKTASWLA
360 370 380 390 400
AELSKEGHQV ALLSGEMMVE QRAAVIERFR EGKEKVLVTT NVCARGIDVE
410 420 430 440 450
QVSVVINFDL PVDKDGNPDN ETYLHRIGRT GRFGKRGLAV NMVDSKHSMN
460 470
ILNRIQEHFN KKIERLDTDD LDEIEKIAN
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BQK0 | H3BQK0_HUMAN | RNA helicase | DDX19B hCG_1998531 | 484 | Annotation score: | ||
H3BMQ5 | H3BMQ5_HUMAN | RNA helicase | DDX19B | 233 | Annotation score: | ||
H3BN59 | H3BN59_HUMAN | RNA helicase | 261 | Annotation score: | |||
F6QDS0 | F6QDS0_HUMAN | RNA helicase | hCG_2043426 | 479 | Annotation score: | ||
H3BPV3 | H3BPV3_HUMAN | ATP-dependent RNA helicase DDX19B | DDX19B | 30 | Annotation score: | ||
H3BUH0 | H3BUH0_HUMAN | ATP-dependent RNA helicase DDX19B | DDX19B | 48 | Annotation score: | ||
H3BTX1 | H3BTX1_HUMAN | ATP-dependent RNA helicase DDX19B | DDX19B | 41 | Annotation score: | ||
H3BMV7 | H3BMV7_HUMAN | ATP-dependent RNA helicase DDX19B | DDX19B | 32 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | A → V in CAG33496 (Ref. 4) Curated | 1 | |
Sequence conflicti | 201 | N → Y in BAG63488 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 331 | I → T in CAG33496 (Ref. 4) Curated | 1 | |
Sequence conflicti | 367 | M → V in CAG38540 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052160 | 149 | V → L. Corresponds to variant dbSNP:rs34607244Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041347 | 1 – 109 | Missing in isoform 3. 3 PublicationsAdd BLAST | 109 | |
Alternative sequenceiVSP_044727 | 1 – 19 | MATDS…AAAES → MAGAAGRVQDRALRRFPITL PVGD in isoform 4. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_015239 | 100 – 130 | Missing in isoform 2 and isoform 4. 3 PublicationsAdd BLAST | 31 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000288071; ENSP00000288071; ENSG00000157349 [Q9UMR2-1] ENST00000355992; ENSP00000348271; ENSG00000157349 [Q9UMR2-2] ENST00000393657; ENSP00000377267; ENSG00000157349 [Q9UMR2-3] ENST00000451014; ENSP00000392639; ENSG00000157349 [Q9UMR2-4] ENST00000563392; ENSP00000456574; ENSG00000157349 [Q9UMR2-3] ENST00000568625; ENSP00000456757; ENSG00000157349 [Q9UMR2-3] |
GeneIDi | 11269 |
KEGGi | hsa:11269 |
UCSCi | uc002eyo.5, human [Q9UMR2-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3EWS | X-ray | 2.70 | A/B | 54-475 | [»] | |
3FHC | X-ray | 2.80 | B | 68-302 | [»] | |
3FHT | X-ray | 2.20 | A/B | 68-479 | [»] | |
3FMO | X-ray | 2.51 | B | 1-300 | [»] | |
3FMP | X-ray | 3.19 | B/D | 1-479 | [»] | |
3G0H | X-ray | 2.70 | A | 54-475 | [»] | |
6B4I | X-ray | 3.62 | E/F | 54-479 | [»] | |
6B4J | X-ray | 3.40 | E/F | 54-479 | [»] | |
6B4K | X-ray | 2.20 | A/B | 54-479 | [»] | |
SMRi | Q9UMR2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116426, 158 interactors |
DIPi | DIP-48486N |
IntActi | Q9UMR2, 94 interactors |
MINTi | Q9UMR2 |
STRINGi | 9606.ENSP00000288071 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q9UMR2 |
PhosphoSitePlusi | Q9UMR2 |
Polymorphism and mutation databases
BioMutai | DDX19B |
DMDMi | 10719979 |
Proteomic databases
EPDi | Q9UMR2 |
jPOSTi | Q9UMR2 |
MassIVEi | Q9UMR2 |
PaxDbi | Q9UMR2 |
PeptideAtlasi | Q9UMR2 |
PRIDEi | Q9UMR2 |
ProteomicsDBi | 16960 85194 [Q9UMR2-1] 85195 [Q9UMR2-2] 85196 [Q9UMR2-3] |
Protocols and materials databases
Antibodypediai | 16335, 306 antibodies |
DNASUi | 11269 |
Genome annotation databases
Ensembli | ENST00000288071; ENSP00000288071; ENSG00000157349 [Q9UMR2-1] ENST00000355992; ENSP00000348271; ENSG00000157349 [Q9UMR2-2] ENST00000393657; ENSP00000377267; ENSG00000157349 [Q9UMR2-3] ENST00000451014; ENSP00000392639; ENSG00000157349 [Q9UMR2-4] ENST00000563392; ENSP00000456574; ENSG00000157349 [Q9UMR2-3] ENST00000568625; ENSP00000456757; ENSG00000157349 [Q9UMR2-3] |
GeneIDi | 11269 |
KEGGi | hsa:11269 |
UCSCi | uc002eyo.5, human [Q9UMR2-1] |
Organism-specific databases
CTDi | 11269 |
DisGeNETi | 11269 |
EuPathDBi | HostDB:ENSG00000157349.16 |
GeneCardsi | DDX19B |
HGNCi | HGNC:2742, DDX19B |
HPAi | ENSG00000157349, Low tissue specificity |
MIMi | 605812, gene |
neXtProti | NX_Q9UMR2 |
OpenTargetsi | ENSG00000157349 |
PharmGKBi | PA27208 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0332, Eukaryota |
GeneTreei | ENSGT00940000154417 |
HOGENOMi | CLU_003041_1_0_1 |
InParanoidi | Q9UMR2 |
OrthoDBi | 608788at2759 |
PhylomeDBi | Q9UMR2 |
TreeFami | TF314957 |
Enzyme and pathway databases
BRENDAi | 3.6.4.13, 2681 |
PathwayCommonsi | Q9UMR2 |
Miscellaneous databases
BioGRID-ORCSi | 11269, 181 hits in 846 CRISPR screens |
ChiTaRSi | DDX19B, human |
EvolutionaryTracei | Q9UMR2 |
GeneWikii | DDX19B |
GenomeRNAii | 11269 |
Pharosi | Q9UMR2, Tbio |
PROi | PR:Q9UMR2 |
RNActi | Q9UMR2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000157349, Expressed in testis and 148 other tissues |
ExpressionAtlasi | Q9UMR2, baseline and differential |
Genevisiblei | Q9UMR2, HS |
Family and domain databases
DisProti | DP01560 |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DD19B_HUMAN | |
Accessioni | Q9UMR2Primary (citable) accession number: Q9UMR2 Secondary accession number(s): B3KNE9 Q9H0U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations