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Protein

ATP-dependent RNA helicase DDX19B

Gene

DDX19B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119ATP1
Binding sitei429ATP1
Binding sitei432ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi138 – 145ATP8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: ProtInc
  • RNA binding Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processmRNA transport, Protein transport, Translocation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.13 2681

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX19B (EC:3.6.4.13)
Alternative name(s):
DEAD box RNA helicase DEAD5
DEAD box protein 19B
Gene namesi
Name:DDX19B
Synonyms:DBP5, DDX19, TDBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000157349.16
HGNCiHGNC:2742 DDX19B
MIMi605812 gene
neXtProtiNX_Q9UMR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi223D → R: Impairs interaction with NUP214 and RNA. 1 Publication1
Mutagenesisi243E → Q: Loss of activity. 1 Publication1
Mutagenesisi258I → A: Impairs interaction with NUP214. 1 Publication1
Mutagenesisi259R → D: Impairs interaction with NUP214. 1 Publication1
Mutagenesisi262R → A: Impairs interaction with NUP214. 1 Publication1

Organism-specific databases

DisGeNETi11269
OpenTargetsiENSG00000157349
PharmGKBiPA27208

Polymorphism and mutation databases

BioMutaiDDX19B
DMDMi10719979

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000550222 – 479ATP-dependent RNA helicase DDX19BAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9UMR2
PaxDbiQ9UMR2
PeptideAtlasiQ9UMR2
PRIDEiQ9UMR2
ProteomicsDBi85194
85195 [Q9UMR2-2]
85196 [Q9UMR2-3]

PTM databases

iPTMnetiQ9UMR2
PhosphoSitePlusiQ9UMR2

Expressioni

Gene expression databases

BgeeiENSG00000157349 Expressed in 131 organ(s), highest expression level in testis
CleanExiHS_DDX19B
ExpressionAtlasiQ9UMR2 baseline and differential
GenevisibleiQ9UMR2 HS

Organism-specific databases

HPAiCAB037229
CAB037284
HPA046380
HPA066668

Interactioni

Subunit structurei

Interacts with NUP214 or RNA in a mutually exclusive manner.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi116426, 153 interactors
DIPiDIP-48486N
IntActiQ9UMR2, 20 interactors
MINTiQ9UMR2
STRINGi9606.ENSP00000288071

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UMR2
SMRiQ9UMR2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UMR2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 295Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini306 – 474Helicase C-terminalPROSITE-ProRule annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 300N-terminal lobeAdd BLAST299
Regioni55 – 68N-terminal helixAdd BLAST14
Regioni301 – 479C-terminal lobeAdd BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 120Q motifAdd BLAST29
Motifi242 – 245DEAD box4

Domaini

The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0332 Eukaryota
ENOG410XRGX LUCA
GeneTreeiENSGT00530000063236
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiQ9UMR2
KOiK18655
PhylomeDBiQ9UMR2
TreeFamiTF314957

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UMR2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATDSWALAV DEQEAAAESL SNLHLKEEKI KPDTNGAVVK TNANAEKTDE
60 70 80 90 100
EEKEDRAAQS LLNKLIRSNL VDNTNQVEVL QRDPNSPLYS VKSFEELRLK
110 120 130 140 150
PQLLQGVYAM GFNRPSKIQE NALPLMLAEP PQNLIAQSQS GTGKTAAFVL
160 170 180 190 200
AMLSQVEPAN KYPQCLCLSP TYELALQTGK VIEQMGKFYP ELKLAYAVRG
210 220 230 240 250
NKLERGQKIS EQIVIGTPGT VLDWCSKLKF IDPKKIKVFV LDEADVMIAT
260 270 280 290 300
QGHQDQSIRI QRMLPRNCQM LLFSATFEDS VWKFAQKVVP DPNVIKLKRE
310 320 330 340 350
EETLDTIKQY YVLCSSRDEK FQALCNLYGA ITIAQAMIFC HTRKTASWLA
360 370 380 390 400
AELSKEGHQV ALLSGEMMVE QRAAVIERFR EGKEKVLVTT NVCARGIDVE
410 420 430 440 450
QVSVVINFDL PVDKDGNPDN ETYLHRIGRT GRFGKRGLAV NMVDSKHSMN
460 470
ILNRIQEHFN KKIERLDTDD LDEIEKIAN
Length:479
Mass (Da):53,927
Last modified:May 1, 2000 - v1
Checksum:i8F6F93B12B7E9871
GO
Isoform 2 (identifier: Q9UMR2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-130: Missing.

Show »
Length:448
Mass (Da):50,490
Checksum:i178321CBF4CA276A
GO
Isoform 3 (identifier: Q9UMR2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-109: Missing.

Show »
Length:370
Mass (Da):41,779
Checksum:iA268A0E259FCF3E2
GO
Isoform 4 (identifier: Q9UMR2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MATDSWALAVDEQEAAAES → MAGAAGRVQDRALRRFPITLPVGD
     100-130: Missing.

Note: No experimental confirmation available.
Show »
Length:453
Mass (Da):51,063
Checksum:i6D543611ED7E4BFE
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BQK0H3BQK0_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B hCG_1998531
484Annotation score:
H3BMQ5H3BMQ5_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B
233Annotation score:
F6QDS0F6QDS0_HUMAN
HCG2043426, isoform CRA_b
hCG_2043426
479Annotation score:
H3BN59H3BN59_HUMAN
Uncharacterized protein
261Annotation score:
H3BTX1H3BTX1_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B
41Annotation score:
H3BMV7H3BMV7_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B
32Annotation score:
H3BPV3H3BPV3_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B
30Annotation score:
H3BUH0H3BUH0_HUMAN
ATP-dependent RNA helicase DDX19B
DDX19B
48Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128A → V in CAG33496 (Ref. 4) Curated1
Sequence conflicti201N → Y in BAG63488 (PubMed:14702039).Curated1
Sequence conflicti331I → T in CAG33496 (Ref. 4) Curated1
Sequence conflicti367M → V in CAG38540 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052160149V → L. Corresponds to variant dbSNP:rs34607244Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0413471 – 109Missing in isoform 3. 3 PublicationsAdd BLAST109
Alternative sequenceiVSP_0447271 – 19MATDS…AAAES → MAGAAGRVQDRALRRFPITL PVGD in isoform 4. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_015239100 – 130Missing in isoform 2 and isoform 4. 3 PublicationsAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237946 mRNA Translation: CAB52189.1
AF353720 mRNA Translation: AAK40102.1
AL136639 mRNA Translation: CAB66574.1
CR457215 mRNA Translation: CAG33496.1
CR533509 mRNA Translation: CAG38540.1
AK027378 mRNA Translation: BAG51311.1
AK301938 mRNA Translation: BAG63358.1
AK302107 mRNA Translation: BAG63488.1
AK316346 mRNA Translation: BAH14717.1
AC012184 Genomic DNA No translation available.
CH471241 Genomic DNA Translation: EAW51827.1
CH471241 Genomic DNA Translation: EAW51831.1
CH471241 Genomic DNA Translation: EAW51832.1
CH471241 Genomic DNA Translation: EAW51834.1
BC003626 mRNA Translation: AAH03626.1
BC010008 mRNA Translation: AAH10008.1
CCDSiCCDS10888.1 [Q9UMR2-1]
CCDS32475.1 [Q9UMR2-2]
CCDS42187.1 [Q9UMR2-3]
CCDS58478.1 [Q9UMR2-4]
RefSeqiNP_001014449.1, NM_001014449.2 [Q9UMR2-3]
NP_001014451.1, NM_001014451.2 [Q9UMR2-2]
NP_001244101.1, NM_001257172.1 [Q9UMR2-4]
NP_001244102.1, NM_001257173.1 [Q9UMR2-3]
NP_001244103.1, NM_001257174.1 [Q9UMR2-3]
NP_009173.1, NM_007242.5 [Q9UMR2-1]
XP_006721190.1, XM_006721127.2 [Q9UMR2-3]
XP_016878379.1, XM_017022890.1
UniGeneiHs.221761

Genome annotation databases

EnsembliENST00000288071; ENSP00000288071; ENSG00000157349 [Q9UMR2-1]
ENST00000355992; ENSP00000348271; ENSG00000157349 [Q9UMR2-2]
ENST00000393657; ENSP00000377267; ENSG00000157349 [Q9UMR2-3]
ENST00000451014; ENSP00000392639; ENSG00000157349 [Q9UMR2-4]
ENST00000563392; ENSP00000456574; ENSG00000157349 [Q9UMR2-3]
ENST00000568625; ENSP00000456757; ENSG00000157349 [Q9UMR2-3]
GeneIDi11269
KEGGihsa:11269
UCSCiuc002eyo.5 human [Q9UMR2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237946 mRNA Translation: CAB52189.1
AF353720 mRNA Translation: AAK40102.1
AL136639 mRNA Translation: CAB66574.1
CR457215 mRNA Translation: CAG33496.1
CR533509 mRNA Translation: CAG38540.1
AK027378 mRNA Translation: BAG51311.1
AK301938 mRNA Translation: BAG63358.1
AK302107 mRNA Translation: BAG63488.1
AK316346 mRNA Translation: BAH14717.1
AC012184 Genomic DNA No translation available.
CH471241 Genomic DNA Translation: EAW51827.1
CH471241 Genomic DNA Translation: EAW51831.1
CH471241 Genomic DNA Translation: EAW51832.1
CH471241 Genomic DNA Translation: EAW51834.1
BC003626 mRNA Translation: AAH03626.1
BC010008 mRNA Translation: AAH10008.1
CCDSiCCDS10888.1 [Q9UMR2-1]
CCDS32475.1 [Q9UMR2-2]
CCDS42187.1 [Q9UMR2-3]
CCDS58478.1 [Q9UMR2-4]
RefSeqiNP_001014449.1, NM_001014449.2 [Q9UMR2-3]
NP_001014451.1, NM_001014451.2 [Q9UMR2-2]
NP_001244101.1, NM_001257172.1 [Q9UMR2-4]
NP_001244102.1, NM_001257173.1 [Q9UMR2-3]
NP_001244103.1, NM_001257174.1 [Q9UMR2-3]
NP_009173.1, NM_007242.5 [Q9UMR2-1]
XP_006721190.1, XM_006721127.2 [Q9UMR2-3]
XP_016878379.1, XM_017022890.1
UniGeneiHs.221761

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EWSX-ray2.70A/B54-475[»]
3FHCX-ray2.80B68-302[»]
3FHTX-ray2.20A/B68-479[»]
3FMOX-ray2.51B1-300[»]
3FMPX-ray3.19B/D1-479[»]
3G0HX-ray2.70A54-475[»]
6B4IX-ray3.62E/F54-479[»]
6B4JX-ray3.40E/F54-479[»]
6B4KX-ray2.20A/B54-479[»]
ProteinModelPortaliQ9UMR2
SMRiQ9UMR2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116426, 153 interactors
DIPiDIP-48486N
IntActiQ9UMR2, 20 interactors
MINTiQ9UMR2
STRINGi9606.ENSP00000288071

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiQ9UMR2
PhosphoSitePlusiQ9UMR2

Polymorphism and mutation databases

BioMutaiDDX19B
DMDMi10719979

Proteomic databases

EPDiQ9UMR2
PaxDbiQ9UMR2
PeptideAtlasiQ9UMR2
PRIDEiQ9UMR2
ProteomicsDBi85194
85195 [Q9UMR2-2]
85196 [Q9UMR2-3]

Protocols and materials databases

DNASUi11269
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000288071; ENSP00000288071; ENSG00000157349 [Q9UMR2-1]
ENST00000355992; ENSP00000348271; ENSG00000157349 [Q9UMR2-2]
ENST00000393657; ENSP00000377267; ENSG00000157349 [Q9UMR2-3]
ENST00000451014; ENSP00000392639; ENSG00000157349 [Q9UMR2-4]
ENST00000563392; ENSP00000456574; ENSG00000157349 [Q9UMR2-3]
ENST00000568625; ENSP00000456757; ENSG00000157349 [Q9UMR2-3]
GeneIDi11269
KEGGihsa:11269
UCSCiuc002eyo.5 human [Q9UMR2-1]

Organism-specific databases

CTDi11269
DisGeNETi11269
EuPathDBiHostDB:ENSG00000157349.16
GeneCardsiDDX19B
HGNCiHGNC:2742 DDX19B
HPAiCAB037229
CAB037284
HPA046380
HPA066668
MIMi605812 gene
neXtProtiNX_Q9UMR2
OpenTargetsiENSG00000157349
PharmGKBiPA27208
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0332 Eukaryota
ENOG410XRGX LUCA
GeneTreeiENSGT00530000063236
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiQ9UMR2
KOiK18655
PhylomeDBiQ9UMR2
TreeFamiTF314957

Enzyme and pathway databases

BRENDAi3.6.4.13 2681

Miscellaneous databases

ChiTaRSiDDX19B human
EvolutionaryTraceiQ9UMR2
GeneWikiiDDX19B
GenomeRNAii11269
PROiPR:Q9UMR2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000157349 Expressed in 131 organ(s), highest expression level in testis
CleanExiHS_DDX19B
ExpressionAtlasiQ9UMR2 baseline and differential
GenevisibleiQ9UMR2 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDD19B_HUMAN
AccessioniPrimary (citable) accession number: Q9UMR2
Secondary accession number(s): B3KNE9
, B4DXS6, E7EMK4, Q6FIB7, Q6IAE0, Q96KE7, Q9H0U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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