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Protein

Spermatogenesis-associated protein 2

Gene

SPATA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (PubMed:27307491, PubMed:27458237, PubMed:27545878, PubMed:27591049). Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC) (PubMed:27458237, PubMed:27545878, PubMed:27591049). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (PubMed:27458237, PubMed:27591049). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (By similarity).By similarity4 Publications

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • spermatogenesis Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processNecrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis-associated protein 22 Publications
Gene namesi
Name:SPATA22 PublicationsImported
Synonyms:KIAA07571 Publication, PD11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000158480.10
HGNCiHGNC:14681 SPATA2
MIMi607662 gene
neXtProtiNX_Q9UM82

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94T → K: Weak or no effect on interaction with CYLD. 1 Publication1
Mutagenesisi98N → A: Weak or no effect on interaction with CYLD. 1 Publication1
Mutagenesisi108F → A: Abolished interaction with CYLD. 1 Publication1
Mutagenesisi114Y → A: Reduced interaction with CYLD. 1 Publication1
Mutagenesisi115T → A or N: Reduced interaction with CYLD. 1 Publication1
Mutagenesisi338Y → A: Strongly reduced interaction with RNF31/HOIP. 1 Publication1

Organism-specific databases

DisGeNETi9825
OpenTargetsiENSG00000158480
PharmGKBiPA37909

Polymorphism and mutation databases

BioMutaiSPATA2
DMDMi13633942

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721001 – 520Spermatogenesis-associated protein 2Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UM82
MaxQBiQ9UM82
PaxDbiQ9UM82
PeptideAtlasiQ9UM82
PRIDEiQ9UM82
ProteomicsDBi85186

PTM databases

iPTMnetiQ9UM82
PhosphoSitePlusiQ9UM82

Expressioni

Tissue specificityi

Present at high level in Sertoli cells, but not detected in spermatogenic cells (at protein level) (PubMed:10222154, PubMed:11079456). Low expression in spleen, thymus and prostate (PubMed:10222154).2 Publications

Gene expression databases

BgeeiENSG00000158480 Expressed in 197 organ(s), highest expression level in secondary oocyte
CleanExiHS_SPATA2
GenevisibleiQ9UM82 HS

Organism-specific databases

HPAiHPA048581
HPA052224

Interactioni

Subunit structurei

Interacts (via the PIM motif) with RNF31/HOIP (via the PUB domain); the interaction is direct (PubMed:27458237, PubMed:27545878, PubMed:28189684, PubMed:27591049). Interacts (via the PUB domain) with CYLD; the interaction is direct (PubMed:27307491, PubMed:27458237, PubMed:27545878, PubMed:27591049).5 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115163, 35 interactors
CORUMiQ9UM82
IntActiQ9UM82, 35 interactors
STRINGi9606.ENSP00000289431

Structurei

Secondary structure

1520
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UM82
SMRiQ9UM82
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 156PUB3 PublicationsAdd BLAST73

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi327 – 344PIM motif2 PublicationsAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 81Poly-Ser6

Sequence similaritiesi

Belongs to the SPATA2 family.Curated

Phylogenomic databases

eggNOGiENOG410IGAI Eukaryota
ENOG410XZJ4 LUCA
GeneTreeiENSGT00530000063956
HOGENOMiHOG000133025
HOVERGENiHBG057357
InParanoidiQ9UM82
KOiK17595
OMAiCSKVSCD
OrthoDBiEOG091G05ZD
PhylomeDBiQ9UM82
TreeFamiTF328840

Family and domain databases

InterProiView protein in InterPro
IPR029744 SPATA2
PANTHERiPTHR15326:SF8 PTHR15326:SF8, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UM82-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKPSSMDTK FKDDLFRKYV QFHESKVDTT TSRQRPGSDE CLRVAASTLL
60 70 80 90 100
SLHKVDPFYR FRLIQFYEVV ESSLRSLSSS SLRALHGAFS MLETVGINLF
110 120 130 140 150
LYPWKKEFRS IKTYTGPFVY YVKSTLLEED IRAILSCMGY TPELGTAYKL
160 170 180 190 200
RELVETLQVK MVSFELFLAK VECEQMLEIH SQVKDKGYSE LDIVSERKSS
210 220 230 240 250
AEDVRGCSDA LRRRAEGREH LTASMSRVAL QKSASERAAK DYYKPRVTKP
260 270 280 290 300
SRSVDAYDSY WESRKPPLKA SLSLRKEPVA TDVGDDLKDE IIRPSPSLLT
310 320 330 340 350
MASSPHGSPD VLPPASPSNG PALLRGTYFS TQDDVDLYTD SEPRATYRRQ
360 370 380 390 400
DALRPDVWLL RNDAHSLYHK RSPPAKESAL SKCQSCGLSC SSSLCQRCDS
410 420 430 440 450
LLTCPPASKP SAFPSKASTH DSLAHGASLR EKYPGQTQGL DRLPHLHSKS
460 470 480 490 500
KPSTTPTSRC GFCNRPGATN TCTQCSKVSC DACLSAYHYD PCYKKSELHK
510 520
FMPNNQLNYK STQLSHLVYR
Length:520
Mass (Da):58,427
Last modified:April 27, 2001 - v2
Checksum:iD29869B601166C89
GO

Sequence cautioni

The sequence BAA34477 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172E → D in AAD28324 (PubMed:10222154).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051368443L → F. Corresponds to variant dbSNP:rs2072985Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28164 mRNA Translation: AAD28324.1
AB018300 mRNA Translation: BAA34477.2 Different initiation.
AL031685 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75645.1
CH471077 Genomic DNA Translation: EAW75646.1
BC009481 mRNA Translation: AAH09481.1
CCDSiCCDS13422.1
RefSeqiNP_001129245.1, NM_001135773.1
NP_006029.1, NM_006038.3
XP_006723957.1, XM_006723894.1
XP_011527418.1, XM_011529116.1
UniGeneiHs.48513

Genome annotation databases

EnsembliENST00000289431; ENSP00000289431; ENSG00000158480
ENST00000422556; ENSP00000416799; ENSG00000158480
GeneIDi9825
KEGGihsa:9825
UCSCiuc002xuw.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28164 mRNA Translation: AAD28324.1
AB018300 mRNA Translation: BAA34477.2 Different initiation.
AL031685 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75645.1
CH471077 Genomic DNA Translation: EAW75646.1
BC009481 mRNA Translation: AAH09481.1
CCDSiCCDS13422.1
RefSeqiNP_001129245.1, NM_001135773.1
NP_006029.1, NM_006038.3
XP_006723957.1, XM_006723894.1
XP_011527418.1, XM_011529116.1
UniGeneiHs.48513

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LJMX-ray1.45A7-219[»]
5LJNX-ray2.70C/D334-341[»]
ProteinModelPortaliQ9UM82
SMRiQ9UM82
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115163, 35 interactors
CORUMiQ9UM82
IntActiQ9UM82, 35 interactors
STRINGi9606.ENSP00000289431

PTM databases

iPTMnetiQ9UM82
PhosphoSitePlusiQ9UM82

Polymorphism and mutation databases

BioMutaiSPATA2
DMDMi13633942

Proteomic databases

EPDiQ9UM82
MaxQBiQ9UM82
PaxDbiQ9UM82
PeptideAtlasiQ9UM82
PRIDEiQ9UM82
ProteomicsDBi85186

Protocols and materials databases

DNASUi9825
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289431; ENSP00000289431; ENSG00000158480
ENST00000422556; ENSP00000416799; ENSG00000158480
GeneIDi9825
KEGGihsa:9825
UCSCiuc002xuw.5 human

Organism-specific databases

CTDi9825
DisGeNETi9825
EuPathDBiHostDB:ENSG00000158480.10
GeneCardsiSPATA2
HGNCiHGNC:14681 SPATA2
HPAiHPA048581
HPA052224
MIMi607662 gene
neXtProtiNX_Q9UM82
OpenTargetsiENSG00000158480
PharmGKBiPA37909
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGAI Eukaryota
ENOG410XZJ4 LUCA
GeneTreeiENSGT00530000063956
HOGENOMiHOG000133025
HOVERGENiHBG057357
InParanoidiQ9UM82
KOiK17595
OMAiCSKVSCD
OrthoDBiEOG091G05ZD
PhylomeDBiQ9UM82
TreeFamiTF328840

Miscellaneous databases

GeneWikiiSPATA2
GenomeRNAii9825
PROiPR:Q9UM82
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158480 Expressed in 197 organ(s), highest expression level in secondary oocyte
CleanExiHS_SPATA2
GenevisibleiQ9UM82 HS

Family and domain databases

InterProiView protein in InterPro
IPR029744 SPATA2
PANTHERiPTHR15326:SF8 PTHR15326:SF8, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPAT2_HUMAN
AccessioniPrimary (citable) accession number: Q9UM82
Secondary accession number(s): E1P626, O94857
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: October 10, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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