PYCARD

Functionⁱ

Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response, acts as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of pro-inflammatory cytokines (PubMed:25847972).

The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16 (PubMed:15030775, PubMed:17349957, PubMed:19158676, PubMed:19158675, PubMed:30674671, PubMed:34678144).

In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered pro-inflammatory responses and inflammasome activation. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590).

21 Publications

Isoform 3:

Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta.

1 Publication

Miscellaneous

In breast tumorigenesis, methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death.

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Apoptosis, Immunity, Inflammatory response, Innate immunity

Biological process

Apoptosis, Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

PathwayCommonsⁱ	Q9ULZ3
Reactomeⁱ	R-HSA-5660668, CLEC7A/inflammasome pathway R-HSA-6798695, Neutrophil degranulation R-HSA-844456, The NLRP3 inflammasome R-HSA-844615, The AIM2 inflammasome R-HSA-9660826, Purinergic signaling in leishmaniasis infection
SignaLinkⁱ	Q9ULZ3
SIGNORⁱ	Q9ULZ3

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Apoptosis-associated speck-like protein containing a CARD Short name: hASC Alternative name(s): Caspase recruitment domain-containing protein 5 PYD and CARD domain-containing protein Target of methylation-induced silencing 11 Publication
Gene namesⁱ	Name:PYCARD Synonyms:ASC, CARD5, TMS11 Publication
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 16

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16608, PYCARD
MIMⁱ	606838, gene
neXtProtⁱ	NX_Q9ULZ3
VEuPathDBⁱ	HostDB:ENSG00000103490

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Inflammasome, Membrane, Mitochondrion, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	8	I → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	12	L → A: Abolishes homooligomerization. 2 Publications	1
Mutagenesisⁱ	12	L → Q: Abolishes promotion of apoptosis and NF-kappa-B activation. 2 Publications	1
Mutagenesisⁱ	13	E → A: Abolishes interaction with PYDC1. 3 Publications	1
Mutagenesisⁱ	13	E → W: Abolishes interaction with NLRP2. 3 Publications	1
Mutagenesisⁱ	15	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	19	E → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	20	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	21	K → A, E or Q: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	23	F → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	25	L → A, E, G, K, N or Q: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	26	K → A or Q: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	27	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	36	Y → A: Abolishes interaction with PYDC1. 1 Publication	1
Mutagenesisⁱ	40	P → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	41	R → A, Q or W: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	45	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	47	M → A, N or Q: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	48	D → A or K: Abolishes homooligomerization. 2 Publications	1
Mutagenesisⁱ	48	D → A: Abolishes interaction with PYDC1. 2 Publications	1
Mutagenesisⁱ	52	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	56	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	62	E → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	67	E → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	68	L → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	72	V → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	76	M → A: Abolishes homooligomerization. 1 Publication	1
Mutagenesisⁱ	174	K → R: Loss of inflammasome activation activity. 1 Publication	1

Keywords - Diseaseⁱ

Tumor suppressor

Organism-specific databases

DisGeNET More...DisGeNETⁱ	29108
Open Targets More...OpenTargetsⁱ	ENSG00000103490
PharmGKBⁱ	PA134950175

Miscellaneous databases

Pharosⁱ	Q9ULZ3, Tbio

Pharosⁱ

Q9ULZ3, Tbio

Genetic variation databases

BioMutaⁱ	PYCARD
DMDMⁱ	18203507

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000064692	1 – 195	Apoptosis-associated speck-like protein containing a CARDAdd BLAST		195

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	174	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residueⁱ	195	PhosphoserineBy similarity		1

Post-translational modificationⁱ

Phosphorylated.1 Publication

'Lys-63'-linked polyubiquitination by TRAF3 is critical for speck formation and inflammasome activation.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9ULZ3
jPOSTⁱ	Q9ULZ3
MassIVEⁱ	Q9ULZ3
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9ULZ3
PaxDbⁱ	Q9ULZ3
PeptideAtlas More...PeptideAtlasⁱ	Q9ULZ3
PRIDEⁱ	Q9ULZ3
ProteomicsDBⁱ	85158 [Q9ULZ3-1] 85159 [Q9ULZ3-2] 85160 [Q9ULZ3-3]

PTM databases

iPTMnetⁱ	Q9ULZ3
PhosphoSitePlusⁱ	Q9ULZ3

Expressionⁱ

Tissue specificityⁱ

Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Expressed in lung epithelial cells (at protein level) (PubMed:23229815). Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.1 Publication

Inductionⁱ

In macrophages, up-regulated by endocannabinoid anandamide/AEA.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000103490, Expressed in monocyte and 205 other tissues
ExpressionAtlasⁱ	Q9ULZ3, baseline and differential
Genevisibleⁱ	Q9ULZ3, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000103490, Tissue enhanced (lymphoid tissue, skin)

HPAⁱ

ENSG00000103490, Tissue enhanced (lymphoid tissue, skin)

Interactionⁱ

Subunit structureⁱ

Self-associates; enforced oligomerization induces apoptosis, NF-kappa-B regulation and interleukin-1 beta secretion (PubMed:15641782, PubMed:17599095, PubMed:33420028, PubMed:33420033). Homooligomers can form disk-like particles of approximately 12 nm diameter and approximately 1 nm height (PubMed:15641782, PubMed:17599095). Next to isoform 1, also isoform 2 and isoform 3 may be involved in oligomerization leading to functional regulation (Probable).

Component of several inflammasomes containing one pattern recognition receptor/sensor, such as NLRP1, NLRP2, NLRP3, NLRP6, NLRC4, AIM2, MEFV or NOD2, and probably NLRC4, NLRP12 or IFI16 (PubMed:11374873, PubMed:12191486, PubMed:15030775, PubMed:15456791, PubMed:19158676, PubMed:30674671, PubMed:34678144). Major component of the ASC pyroptosome, a 1-2 um supramolecular assembly (one per macrophage cell) which consists of oligomerized PYCARD dimers and CASP1 (PubMed:17599095).

Interacts with CASP1 (precursor form); the interaction induces activation of CASP1 leading to the processing of interleukin-1 beta; PYCARD competes with RIPK2 for binding to CASP1 (PubMed:11967258, PubMed:14634131, PubMed:16585594, PubMed:17599095, PubMed:33420033).

Interacts with NLRP3; the interaction requires the homooligomerization of NLRP3 (PubMed:11786556, PubMed:15030775, PubMed:15020601, PubMed:34341353).

Interacts with NLRP2, NLRC4, MEFV, CARD16, AIM2, IFI16, NOD2, DDX58, RIPK2, PYDC1, PYDC2, NLRP10, CASP8, CHUK, IKBKB and BAX (PubMed:11374873, PubMed:11498534, PubMed:12486103, PubMed:12646168, PubMed:12656673, PubMed:15456791, PubMed:14730312, PubMed:15096476, PubMed:17178784, PubMed:17339483, PubMed:18362139, PubMed:19158675, PubMed:19158676, PubMed:19915568, PubMed:21575908).

Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity).

By similarityCurated29 Publications

Binary interactionsⁱ

Hide details

Q9ULZ3

With	#Exp.	IntAct
AIM2 [O14862]	13	EBI-751215,EBI-6253193
BAX [Q07812]	7	EBI-751215,EBI-516580
CASP1 [P29466]	9	EBI-751215,EBI-516667
EXOC5 [O00471]	5	EBI-751215,EBI-949824
MEFV [O15553]	8	EBI-751215,EBI-7644532
NLRC3 [Q7RTR2]	3	EBI-751215,EBI-1042625
NLRP1 [Q9C000]	5	EBI-751215,EBI-1220518
NLRP3 [Q96P20]	24	EBI-751215,EBI-6253230
itself	8	EBI-751215,EBI-751215
PYDC2 [Q56P42]	4	EBI-751215,EBI-6374418
RIPK1 [Q13546]	2	EBI-751215,EBI-358507
SYK [P43405]	4	EBI-751215,EBI-78302

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	118876, 78 interactors
ComplexPortalⁱ	CPX-4082, NLRP1 inflammasome CPX-4141, NLRP3 inflammasome CPX-4142, AIM2 inflammasome CPX-4143, Pyrin inflammasome CPX-4144, NLRC4 inflammasome
CORUMⁱ	Q9ULZ3
Database of interacting proteins More...DIPⁱ	DIP-27618N
IntActⁱ	Q9ULZ3, 56 interactors
Molecular INTeraction database More...MINTⁱ	Q9ULZ3
STRINGⁱ	9606.ENSP00000247470

Miscellaneous databases

RNActⁱ	Q9ULZ3, protein

RNActⁱ

Q9ULZ3, protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 14	Combined sources	12
Helixⁱ	17 – 26	Combined sources	10
Turnⁱ	27 – 29	Combined sources	3
Beta strandⁱ	34 – 36	Combined sources	3
Helixⁱ	41 – 46	Combined sources	6
Helixⁱ	49 – 58	Combined sources	10
Helixⁱ	62 – 75	Combined sources	14
Helixⁱ	80 – 90	Combined sources	11
Helixⁱ	114 – 117	Combined sources	4
Helixⁱ	119 – 125	Combined sources	7
Helixⁱ	129 – 136	Combined sources	8
Turnⁱ	138 – 140	Combined sources	3
Helixⁱ	143 – 150	Combined sources	8
Beta strandⁱ	152 – 154	Combined sources	3
Helixⁱ	155 – 164	Combined sources	10
Helixⁱ	165 – 168	Combined sources	4
Helixⁱ	171 – 184	Combined sources	14
Helixⁱ	186 – 195	Combined sources	10

Show more details

3D structure databases

AlphaFoldDBⁱ	Q9ULZ3
BMRBⁱ	Q9ULZ3
SMRⁱ	Q9ULZ3
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9ULZ3

EvolutionaryTraceⁱ

Q9ULZ3

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 91	PyrinPROSITE-ProRule annotationAdd BLAST		91
Domainⁱ	107 – 195	CARDPROSITE-ProRule annotationAdd BLAST		89

Domainⁱ

The CARD domain mediates interaction with CASP1 and NLRC4 (PubMed:14634131 and PubMed:11967258).1 Publication

The pyrin domain mediates homotypic interactions with pyrin domains of proteins such as of NLRP3, PYDC1, PYDC2 and AIM2.6 Publications

Phylogenomic databases

eggNOGⁱ	ENOG502S3G5, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000161873
HOGENOMⁱ	CLU_113553_1_0_1
InParanoidⁱ	Q9ULZ3
OMAⁱ	NPAKMRK
Database of Orthologous Groups More...OrthoDBⁱ	1494108at2759
PhylomeDBⁱ	Q9ULZ3
TreeFamⁱ	TF337882

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd08330, CARD_ASC_NALP1, 1 hit
Gene3Dⁱ	1.10.533.10, 2 hits
InterProⁱ	View protein in InterPro IPR001315, CARD IPR033516, CARD8/ASC/NALP1_CARD IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR002398, Pept_C14
PANTHERⁱ	PTHR10454, PTHR10454, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00619, CARD, 1 hit PF02758, PYRIN, 1 hit
SMARTⁱ	View protein in SMART SM01289, PYRIN, 1 hit
SUPFAMⁱ	SSF47986, SSF47986, 2 hits
PROSITEⁱ	View protein in PROSITE PS50209, CARD, 1 hit PS50824, DAPIN, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: Q9ULZ3-1) [UniParc]FASTA Add to basket

Also known as: fASC

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL 
        60         70         80         90        100
DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI 
       110        120        130        140        150
QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYGKV LTDEQYQAVR 
       160        170        180        190 
AEPTNPSKMR KLFSFTPAWN WTCKDLLLQA LRESQSYLVE DLERS

Length:195

Mass (Da):21,627

Last modified:March 1, 2001 - v2

Checksum:ⁱ455987286586F46A

GO

Isoform 2 (identifier: Q9ULZ3-2) [UniParc]FASTA Add to basket

Also known as: Asc-b, vASC

The sequence of this isoform differs from the canonical sequence as follows:
93-111: Missing.

« Hide

        10         20         30         40         50
MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL 
        60         70         80         90        100
DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGLHFIDQHR 
       110        120        130        140        150
AALIARVTNV EWLLDALYGK VLTDEQYQAV RAEPTNPSKM RKLFSFTPAW 
       160        170 
NWTCKDLLLQ ALRESQSYLV EDLERS

Show »

Length:176

Mass (Da):19,969

Checksum:ⁱC4AB645696FA115D

GO

Isoform 3 (identifier: Q9ULZ3-3) [UniParc]FASTA Add to basket

Also known as: Asc-c

The sequence of this isoform differs from the canonical sequence as follows:
26-85: Missing.

« Hide

        10         20         30         40         50
MGRARDAILD ALENLTAEEL KKFKLQAATH QGSGAAPAGI QAPPQSAAKP 
        60         70         80         90        100
GLHFIDQHRA ALIARVTNVE WLLDALYGKV LTDEQYQAVR AEPTNPSKMR 
       110        120        130 
KLFSFTPAWN WTCKDLLLQA LRESQSYLVE DLERS

Show »

Length:135

Mass (Da):15,030

Checksum:ⁱF46DB2DF379E95E9

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	H3BP42	H3BP42_HUMAN		Apoptosis-associated speck-like pro... Apoptosis-associated speck-like protein-containing a CARD	PYCARD	125	Annotation score:

Sequence cautionⁱ

The sequence BAA91012 differs from that shown. Reason: Frameshift.Curated

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_004118	26 – 85	Missing in isoform 3. 1 PublicationAdd BLAST		60
Alternative sequenceⁱVSP_004119	93 – 111	Missing in isoform 2. 1 PublicationAdd BLAST		19

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB023416 mRNA Translation: BAA87339.2 AF184072 Genomic DNA Translation: AAG01187.1 AF184073 mRNA Translation: AAG01188.1 AF255794 mRNA Translation: AAF99665.1 AF310103 mRNA Translation: AAG30286.1 AF384665 mRNA Translation: AAK63850.1 AK000211 mRNA Translation: BAA91012.1 Frameshift. BC004470 mRNA Translation: AAH04470.1 BC013569 mRNA Translation: AAH13569.2
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10708.1 [Q9ULZ3-1] CCDS10709.1 [Q9ULZ3-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_037390.2, NM_013258.4 [Q9ULZ3-1] NP_660183.1, NM_145182.2 [Q9ULZ3-2]

Genome annotation databases

Ensemblⁱ	ENST00000247470.10; ENSP00000247470.9; ENSG00000103490.14 ENST00000350605.4; ENSP00000340441.4; ENSG00000103490.14 [Q9ULZ3-2]
GeneIDⁱ	29108
KEGGⁱ	hsa:29108
MANE-Selectⁱ	ENST00000247470.10; ENSP00000247470.9; NM_013258.5; NP_037390.2
UCSC genome browser More...UCSCⁱ	uc002ebm.4, human [Q9ULZ3-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9ULZ3	PYD and CARD domain containing		GORGO		195	UniRef100_Q9ULZ3
+1

Protein	Similar proteins		Species	Score	Length	Source
Q9ULZ3	PYD and CARD domain containing		GORGO		195	UniRef90_Q9ULZ3
Uncharacterized protein		RHIRO		195
Uncharacterized protein		RHIBE		195
PYD and CARD domain containing		COLAP		195
PYD and CARD domain containing		Ugandan red Colobus		195
+23

Protein	Similar proteins		Species	Score	Length	Source
Q9ULZ3	Apoptosis-associated speck-like protein containing a CARD		BOVIN		195	UniRef50_Q9ULZ3
PYD and CARD domain containing		GORGO		195
PYD and CARD domain containing		MACNE		195
Uncharacterized protein		CHLSB		195
Uncharacterized protein		RHIRO		195
+70

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB023416 mRNA Translation: BAA87339.2 AF184072 Genomic DNA Translation: AAG01187.1 AF184073 mRNA Translation: AAG01188.1 AF255794 mRNA Translation: AAF99665.1 AF310103 mRNA Translation: AAG30286.1 AF384665 mRNA Translation: AAK63850.1 AK000211 mRNA Translation: BAA91012.1 Frameshift. BC004470 mRNA Translation: AAH04470.1 BC013569 mRNA Translation: AAH13569.2
CCDSⁱ	CCDS10708.1 [Q9ULZ3-1] CCDS10709.1 [Q9ULZ3-2]
RefSeqⁱ	NP_037390.2, NM_013258.4 [Q9ULZ3-1] NP_660183.1, NM_145182.2 [Q9ULZ3-2]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1UCP	NMR	-	A	1-91	[»]
	2KN6	NMR	-	A	1-195	[»]
	3J63	electron microscopy	3.80	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O	1-91	[»]
	5H8O	X-ray	4.21	B	115-195	[»]
	6K99	electron microscopy	4.10	A/B/C/D/E/F/G/H/I/J/K/L	112-194	[»]
	6KI0	X-ray	2.00	A/B	112-195	[»]
	6N1H	electron microscopy	3.17	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P	112-194	[»]
	7KEU	electron microscopy	3.90	A/B/C/D	113-194	[»]
AlphaFoldDBⁱ	Q9ULZ3
BMRBⁱ	Q9ULZ3
SMRⁱ	Q9ULZ3
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	118876, 78 interactors
ComplexPortalⁱ	CPX-4082, NLRP1 inflammasome CPX-4141, NLRP3 inflammasome CPX-4142, AIM2 inflammasome CPX-4143, Pyrin inflammasome CPX-4144, NLRC4 inflammasome
CORUMⁱ	Q9ULZ3
DIPⁱ	DIP-27618N
IntActⁱ	Q9ULZ3, 56 interactors
MINTⁱ	Q9ULZ3
STRINGⁱ	9606.ENSP00000247470

PTM databases

iPTMnetⁱ	Q9ULZ3
PhosphoSitePlusⁱ	Q9ULZ3

Genetic variation databases

BioMutaⁱ	PYCARD
DMDMⁱ	18203507

Proteomic databases

EPDⁱ	Q9ULZ3
jPOSTⁱ	Q9ULZ3
MassIVEⁱ	Q9ULZ3
MaxQBⁱ	Q9ULZ3
PaxDbⁱ	Q9ULZ3
PeptideAtlasⁱ	Q9ULZ3
PRIDEⁱ	Q9ULZ3
ProteomicsDBⁱ	85158 [Q9ULZ3-1] 85159 [Q9ULZ3-2] 85160 [Q9ULZ3-3]

Protocols and materials databases

ABCDⁱ	Q9ULZ3, 1 sequenced antibody
Antibodypediaⁱ	3375, 729 antibodies from 44 providers
The DNASU plasmid repository More...DNASUⁱ	29108

Genome annotation databases

Ensemblⁱ	ENST00000247470.10; ENSP00000247470.9; ENSG00000103490.14 ENST00000350605.4; ENSP00000340441.4; ENSG00000103490.14 [Q9ULZ3-2]
GeneIDⁱ	29108
KEGGⁱ	hsa:29108
MANE-Selectⁱ	ENST00000247470.10; ENSP00000247470.9; NM_013258.5; NP_037390.2
UCSCⁱ	uc002ebm.4, human [Q9ULZ3-1]

Organism-specific databases

CTDⁱ	29108
DisGeNETⁱ	29108
GeneCardsⁱ	PYCARD
HGNCⁱ	HGNC:16608, PYCARD
HPAⁱ	ENSG00000103490, Tissue enhanced (lymphoid tissue, skin)
MIMⁱ	606838, gene
neXtProtⁱ	NX_Q9ULZ3
OpenTargetsⁱ	ENSG00000103490
PharmGKBⁱ	PA134950175
VEuPathDBⁱ	HostDB:ENSG00000103490
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG502S3G5, Eukaryota
GeneTreeⁱ	ENSGT00940000161873
HOGENOMⁱ	CLU_113553_1_0_1
InParanoidⁱ	Q9ULZ3
OMAⁱ	NPAKMRK
OrthoDBⁱ	1494108at2759
PhylomeDBⁱ	Q9ULZ3
TreeFamⁱ	TF337882

Enzyme and pathway databases

PathwayCommonsⁱ	Q9ULZ3
Reactomeⁱ	R-HSA-5660668, CLEC7A/inflammasome pathway R-HSA-6798695, Neutrophil degranulation R-HSA-844456, The NLRP3 inflammasome R-HSA-844615, The AIM2 inflammasome R-HSA-9660826, Purinergic signaling in leishmaniasis infection
SignaLinkⁱ	Q9ULZ3
SIGNORⁱ	Q9ULZ3

Miscellaneous databases

BioGRID-ORCSⁱ	29108, 11 hits in 1084 CRISPR screens
ChiTaRSⁱ	PYCARD, human
EvolutionaryTraceⁱ	Q9ULZ3
GeneWikiⁱ	PYCARD
GenomeRNAiⁱ	29108
Pharosⁱ	Q9ULZ3, Tbio
Protein Ontology More...PROⁱ	PR:Q9ULZ3
RNActⁱ	Q9ULZ3, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000103490, Expressed in monocyte and 205 other tissues
ExpressionAtlasⁱ	Q9ULZ3, baseline and differential
Genevisibleⁱ	Q9ULZ3, HS

Family and domain databases

CDDⁱ	cd08330, CARD_ASC_NALP1, 1 hit
Gene3Dⁱ	1.10.533.10, 2 hits
InterProⁱ	View protein in InterPro IPR001315, CARD IPR033516, CARD8/ASC/NALP1_CARD IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR002398, Pept_C14
PANTHERⁱ	PTHR10454, PTHR10454, 1 hit
Pfamⁱ	View protein in Pfam PF00619, CARD, 1 hit PF02758, PYRIN, 1 hit
SMARTⁱ	View protein in SMART SM01289, PYRIN, 1 hit
SUPFAMⁱ	SSF47986, SSF47986, 2 hits
PROSITEⁱ	View protein in PROSITE PS50209, CARD, 1 hit PS50824, DAPIN, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASC_HUMAN
Accessionⁱ	Q9ULZ3Primary (citable) accession number: Q9ULZ3 Secondary accession number(s): Q96D12 , Q9BSZ5, Q9HBD0, Q9NXJ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
	Last sequence update:	March 1, 2001
	Last modified:	May 25, 2022
	This is version 205 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q9ULZ3 (ASC_HUMAN)

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Apoptosis-associated speck-like protein containing a CARD

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

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Keywords - PTMi

Proteomic databases

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Q9ULZ3

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<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Alternative sequence

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Keywords - Coding sequence diversityi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ