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Protein

A-kinase anchor protein 8-like

Gene

AKAP8L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).1 Publication6 Publications
(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.1 Publication
(Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.1 Publication
(Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri391 – 413C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 507C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: UniProtKB
  • DNA binding Source: InterPro
  • histone deacetylase binding Source: UniProtKB
  • lamin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein kinase A regulatory subunit binding Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 8-like
Short name:
AKAP8-like protein
Alternative name(s):
Helicase A-binding protein 95
Short name:
HAP95
Homologous to AKAP95 protein
Short name:
HA95
Neighbor of A-kinase-anchoring protein 95
Short name:
Neighbor of AKAP95
Gene namesi
Name:AKAP8L
Synonyms:NAKAP, NAKAP95
ORF Names:HRIHFB2018
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000011243.17
HGNCiHGNC:29857 AKAP8L
MIMi609475 gene
neXtProtiNX_Q9ULX6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26993
OpenTargetsiENSG00000011243
PharmGKBiPA134867364

Polymorphism and mutation databases

BioMutaiAKAP8L
DMDMi296439446

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000753841 – 646A-kinase anchor protein 8-likeAdd BLAST646

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei208Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei208Omega-N-methylarginine; alternateCombined sources1
Modified residuei217Omega-N-methylarginineCombined sources1
Modified residuei237Omega-N-methylarginineCombined sources1
Modified residuei247Omega-N-methylarginineCombined sources1
Modified residuei257N6-acetyllysineCombined sources1
Modified residuei267PhosphothreonineCombined sources1
Modified residuei283PhosphoserineCombined sources1
Modified residuei292PhosphothreonineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei552PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9ULX6
PaxDbiQ9ULX6
PeptideAtlasiQ9ULX6
PRIDEiQ9ULX6
ProteomicsDBi85148

PTM databases

iPTMnetiQ9ULX6
PhosphoSitePlusiQ9ULX6

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in the brain cortex (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000011243 Expressed in 215 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_AKAP8L
ExpressionAtlasiQ9ULX6 baseline and differential
GenevisibleiQ9ULX6 HS

Organism-specific databases

HPAiHPA042485
HPA042546

Interactioni

Subunit structurei

Interacts (via N-terminus) with DHX9 (via RGG region) (PubMed:10748171, PubMed:11402034). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B (PubMed:11034899, PubMed:12538639 PubMed:16391387, PubMed:18313049). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with EBV EBNA-LP (PubMed:11160753). Interacts with HIV-1 reverse transcriptase/ribonuclease H (PubMed:25034436).8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117940, 42 interactors
CORUMiQ9ULX6
DIPiDIP-27541N
IntActiQ9ULX6, 89 interactors
MINTiQ9ULX6
STRINGi9606.ENSP00000380557

Structurei

3D structure databases

ProteinModelPortaliQ9ULX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 268Sufficient for activation of CTE-mediated expression1 PublicationAdd BLAST268

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 279Nuclear localization signalSequence analysis6
Motifi280 – 296Nuclear export signal (NES)1 PublicationAdd BLAST17
Motifi362 – 364Nuclear localization signalSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 52Gly/Tyr-richAdd BLAST12
Compositional biasi589 – 597Pro-rich9
Compositional biasi602 – 608Pro-rich7

Sequence similaritiesi

Belongs to the AKAP95 family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri391 – 413C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 507C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIFX Eukaryota
ENOG410YCRZ LUCA
GeneTreeiENSGT00530000063777
HOGENOMiHOG000169111
HOVERGENiHBG104297
InParanoidiQ9ULX6
KOiK15978
OrthoDBiEOG091G0836
PhylomeDBiQ9ULX6
TreeFamiTF105407

Family and domain databases

InterProiView protein in InterPro
IPR007071 AKAP95
IPR034736 ZF_C2H2_AKAP95
PANTHERiPTHR12190 PTHR12190, 1 hit
PfamiView protein in Pfam
PF04988 AKAP95, 1 hit
PROSITEiView protein in PROSITE
PS51799 ZF_C2H2_AKAP95, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9ULX6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSYTGFVQGS ETTLQSTYSD TSAQPTCDYG YGTWNSGTNR GYEGYGYGYG
60 70 80 90 100
YGQDNTTNYG YGMATSHSWE MPSSDTNANT SASGSASADS VLSRINQRLD
110 120 130 140 150
MVPHLETDMM QGGVYGSGGE RYDSYESCDS RAVLSERDLY RSGYDYSELD
160 170 180 190 200
PEMEMAYEGQ YDAYRDQFRM RGNDTFGPRA QGWARDARSG RPMASGYGRM
210 220 230 240 250
WEDPMGARGQ CMSGASRLPS LFSQNIIPEY GMFQGMRGGG AFPGGSRFGF
260 270 280 290 300
GFGNGMKQMR RTWKTWTTAD FRTKKKKRKQ GGSPDEPDSK ATRTDCSDNS
310 320 330 340 350
DSDNDEGTEG EATEGLEGTE AVEKGSRVDG EDEEGKEDGR EEGKEDPEKG
360 370 380 390 400
ALTTQDENGQ TKRKLQAGKK SQDKQKKRQR DRMVERIQFV CSLCKYRTFY
410 420 430 440 450
EDEMASHLDS KFHKEHFKYV GTKLPKQTAD FLQEYVTNKT KKTEELRKTV
460 470 480 490 500
EDLDGLIQQI YRDQDLTQEI AMEHFVKKVE AAHCAACDLF IPMQFGIIQK
510 520 530 540 550
HLKTMDHNRN RRLMMEQSKK SSLMVARSIL NNKLISKKLE RYLKGENPFT
560 570 580 590 600
DSPEEEKEQE EAEGGALDEG AQGEAAGISE GAEGVPAQPP VPPEPAPGAV
610 620 630 640
SPPPPPPPEE EEEGAVPLLG GALQRQIRGI PGLDVEDDEE GGGGAP
Length:646
Mass (Da):71,640
Last modified:September 12, 2018 - v4
Checksum:iCBC265BF25996BA2
GO
Isoform 2 (identifier: Q9ULX6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-121: Missing.

Show »
Length:585
Mass (Da):65,221
Checksum:i6315372DFB1DAF9E
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V9GZ50V9GZ50_HUMAN
A kinase (PRKA) anchor protein 8-li...
AKAP8L hCG_36782
431Annotation score:
M0R010M0R010_HUMAN
A-kinase anchor protein 8-like
AKAP8L
161Annotation score:
M0QYT7M0QYT7_HUMAN
A-kinase anchor protein 8-like
AKAP8L
162Annotation score:
M0R008M0R008_HUMAN
A-kinase anchor protein 8-like
AKAP8L
66Annotation score:
M0R1Y5M0R1Y5_HUMAN
A-kinase anchor protein 8-like
AKAP8L
109Annotation score:
M0R1L5M0R1L5_HUMAN
A-kinase anchor protein 8-like
AKAP8L
45Annotation score:

Sequence cautioni

The sequence AAF86048 differs from that shown. Reason: Frameshift at position 553.Curated
The sequence BAA34791 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100D → N in AAF86048 (PubMed:10748171).Curated1
Sequence conflicti189S → N in AAF86048 (PubMed:10748171).Curated1
Sequence conflicti351 – 358ALTTQDEN → EFSWGAWC in BAA34791 (PubMed:9853615).Curated8
Sequence conflicti599 – 616AVSPP…EEGAV → GRVAATAAAPRRRRRRAPW in AAF86048 (PubMed:10748171).CuratedAdd BLAST18
Sequence conflicti641 – 646GGGGAP → EGRRGRPV in AAF86048 (PubMed:10748171).Curated6

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_068822458Q → H1 PublicationCorresponds to variant dbSNP:rs2058322Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04442661 – 121Missing in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025905 mRNA Translation: BAA85003.1
AJ243467 mRNA Translation: CAB65092.1
AF199414 mRNA Translation: AAF86048.1 Frameshift.
AK295956 mRNA Translation: BAG58736.1
AB451326 mRNA Translation: BAG70140.1
AB451469 mRNA Translation: BAG70283.1
AC005785 Genomic DNA No translation available.
AC006128 Genomic DNA No translation available.
KF456508 Genomic DNA No translation available.
KF456511 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84475.1
BC000713 mRNA Translation: AAH00713.1
AB015332 mRNA Translation: BAA34791.1 Different initiation.
CCDSiCCDS46005.1 [Q9ULX6-1]
CCDS77249.1 [Q9ULX6-2]
RefSeqiNP_001278407.1, NM_001291478.1 [Q9ULX6-2]
NP_055186.3, NM_014371.3 [Q9ULX6-1]
UniGeneiHs.399800

Genome annotation databases

EnsembliENST00000397410; ENSP00000380557; ENSG00000011243 [Q9ULX6-1]
ENST00000595465; ENSP00000470952; ENSG00000011243 [Q9ULX6-2]
GeneIDi26993
KEGGihsa:26993
UCSCiuc002naw.2 human [Q9ULX6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025905 mRNA Translation: BAA85003.1
AJ243467 mRNA Translation: CAB65092.1
AF199414 mRNA Translation: AAF86048.1 Frameshift.
AK295956 mRNA Translation: BAG58736.1
AB451326 mRNA Translation: BAG70140.1
AB451469 mRNA Translation: BAG70283.1
AC005785 Genomic DNA No translation available.
AC006128 Genomic DNA No translation available.
KF456508 Genomic DNA No translation available.
KF456511 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84475.1
BC000713 mRNA Translation: AAH00713.1
AB015332 mRNA Translation: BAA34791.1 Different initiation.
CCDSiCCDS46005.1 [Q9ULX6-1]
CCDS77249.1 [Q9ULX6-2]
RefSeqiNP_001278407.1, NM_001291478.1 [Q9ULX6-2]
NP_055186.3, NM_014371.3 [Q9ULX6-1]
UniGeneiHs.399800

3D structure databases

ProteinModelPortaliQ9ULX6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117940, 42 interactors
CORUMiQ9ULX6
DIPiDIP-27541N
IntActiQ9ULX6, 89 interactors
MINTiQ9ULX6
STRINGi9606.ENSP00000380557

PTM databases

iPTMnetiQ9ULX6
PhosphoSitePlusiQ9ULX6

Polymorphism and mutation databases

BioMutaiAKAP8L
DMDMi296439446

Proteomic databases

EPDiQ9ULX6
PaxDbiQ9ULX6
PeptideAtlasiQ9ULX6
PRIDEiQ9ULX6
ProteomicsDBi85148

Protocols and materials databases

DNASUi26993
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397410; ENSP00000380557; ENSG00000011243 [Q9ULX6-1]
ENST00000595465; ENSP00000470952; ENSG00000011243 [Q9ULX6-2]
GeneIDi26993
KEGGihsa:26993
UCSCiuc002naw.2 human [Q9ULX6-1]

Organism-specific databases

CTDi26993
DisGeNETi26993
EuPathDBiHostDB:ENSG00000011243.17
GeneCardsiAKAP8L
H-InvDBiHIX0014850
HIX0115658
HGNCiHGNC:29857 AKAP8L
HPAiHPA042485
HPA042546
MIMi609475 gene
neXtProtiNX_Q9ULX6
OpenTargetsiENSG00000011243
PharmGKBiPA134867364
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIFX Eukaryota
ENOG410YCRZ LUCA
GeneTreeiENSGT00530000063777
HOGENOMiHOG000169111
HOVERGENiHBG104297
InParanoidiQ9ULX6
KOiK15978
OrthoDBiEOG091G0836
PhylomeDBiQ9ULX6
TreeFamiTF105407

Miscellaneous databases

ChiTaRSiAKAP8L human
GeneWikiiAKAP8L
GenomeRNAii26993
PROiPR:Q9ULX6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000011243 Expressed in 215 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_AKAP8L
ExpressionAtlasiQ9ULX6 baseline and differential
GenevisibleiQ9ULX6 HS

Family and domain databases

InterProiView protein in InterPro
IPR007071 AKAP95
IPR034736 ZF_C2H2_AKAP95
PANTHERiPTHR12190 PTHR12190, 1 hit
PfamiView protein in Pfam
PF04988 AKAP95, 1 hit
PROSITEiView protein in PROSITE
PS51799 ZF_C2H2_AKAP95, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAKP8L_HUMAN
AccessioniPrimary (citable) accession number: Q9ULX6
Secondary accession number(s): B4DJ74
, B5BU90, O94792, Q96J58, Q9NRQ0, Q9UGM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 12, 2018
Last modified: November 7, 2018
This is version 176 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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