UniProtKB - Q9ULX6 (AKP8L_HUMAN)
A-kinase anchor protein 8-like
AKAP8L
Functioni
Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034).
Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601).
May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899).
May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639).
Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585).
May be involved in regulation of pre-mRNA splicing (PubMed:17594903).
1 Publication6 Publications(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.
1 Publication(Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.
1 Publication(Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 391 – 413 | C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 484 – 507 | C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DEAD/H-box RNA helicase binding Source: UniProtKB
- DNA binding Source: InterPro
- histone deacetylase binding Source: UniProtKB
- lamin binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein kinase A regulatory subunit binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- cell cycle G2/M phase transition Source: UniProtKB
- mitotic chromosome condensation Source: UniProtKB
- mRNA processing Source: UniProtKB
- nuclear membrane disassembly Source: UniProtKB
- positive regulation of histone deacetylation Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of histone phosphorylation Source: UniProtKB
- regulation of mRNA export from nucleus Source: UniProtKB
- RNA splicing Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction, mRNA processing, mRNA splicing, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9ULX6 |
SignaLinki | Q9ULX6 |
SIGNORi | Q9ULX6 |
Names & Taxonomyi
Protein namesi | Recommended name: A-kinase anchor protein 8-likeShort name: AKAP8-like protein Alternative name(s): Helicase A-binding protein 95 Short name: HAP95 Homologous to AKAP95 protein Short name: HA95 Neighbor of A-kinase-anchoring protein 95 Short name: Neighbor of AKAP95 |
Gene namesi | Name:AKAP8L Synonyms:NAKAP, NAKAP95 ORF Names:HRIHFB2018 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29857, AKAP8L |
MIMi | 609475, gene |
neXtProti | NX_Q9ULX6 |
VEuPathDBi | HostDB:ENSG00000011243 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
- Nucleus matrix 2 Publications
- Nucleus speckle 1 Publication
- PML body 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899). Colocalizes with EBNA-LP in PML bodies (PubMed:11884601).4 Publications
Nucleus
- nuclear matrix Source: UniProtKB
- nuclear speck Source: UniProtKB
- nucleus Source: UniProtKB
- PML body Source: UniProtKB-SubCell
Other locations
- chromatin Source: UniProtKB
- cytoplasm Source: UniProtKB
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 26993 |
OpenTargetsi | ENSG00000011243 |
PharmGKBi | PA134867364 |
Miscellaneous databases
Pharosi | Q9ULX6, Tbio |
Genetic variation databases
BioMutai | AKAP8L |
DMDMi | 296439446 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075384 | 1 – 646 | A-kinase anchor protein 8-likeAdd BLAST | 646 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 208 | Asymmetric dimethylarginine; alternateCombined sources | 1 | |
Modified residuei | 208 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 217 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 237 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 247 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 257 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 267 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 283 | PhosphoserineCombined sources | 1 | |
Modified residuei | 292 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 297 | PhosphoserineCombined sources | 1 | |
Modified residuei | 552 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q9ULX6 |
jPOSTi | Q9ULX6 |
MassIVEi | Q9ULX6 |
MaxQBi | Q9ULX6 |
PaxDbi | Q9ULX6 |
PeptideAtlasi | Q9ULX6 |
PRIDEi | Q9ULX6 |
ProteomicsDBi | 85148 [Q9ULX6-1] |
PTM databases
GlyGeni | Q9ULX6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9ULX6 |
PhosphoSitePlusi | Q9ULX6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000011243, Expressed in left lobe of thyroid gland and 230 other tissues |
ExpressionAtlasi | Q9ULX6, baseline and differential |
Genevisiblei | Q9ULX6, HS |
Organism-specific databases
HPAi | ENSG00000011243, Low tissue specificity |
Interactioni
Subunit structurei
Interacts (via N-terminus) with DHX9 (via RGG region) (PubMed:10748171, PubMed:11402034).
Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B (PubMed:11034899, PubMed:12538639 PubMed:16391387, PubMed:18313049).
Interacts with HDAC3; increased during mitosis (PubMed:16980585).
Interacts with EBV EBNA-LP (PubMed:11160753).
Interacts with HIV-1 reverse transcriptase/ribonuclease H (PubMed:25034436).
8 PublicationsBinary interactionsi
Q9ULX6
GO - Molecular functioni
- DEAD/H-box RNA helicase binding Source: UniProtKB
- histone deacetylase binding Source: UniProtKB
- lamin binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 117940, 160 interactors |
CORUMi | Q9ULX6 |
DIPi | DIP-27541N |
IntActi | Q9ULX6, 157 interactors |
MINTi | Q9ULX6 |
STRINGi | 9606.ENSP00000380557 |
Miscellaneous databases
RNActi | Q9ULX6, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 268 | Sufficient for activation of CTE-mediated expression1 PublicationAdd BLAST | 268 | |
Regioni | 264 – 381 | DisorderedSequence analysisAdd BLAST | 118 | |
Regioni | 545 – 646 | DisorderedSequence analysisAdd BLAST | 102 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 274 – 279 | Nuclear localization signalSequence analysis | 6 | |
Motifi | 280 – 296 | Nuclear export signal (NES)1 PublicationAdd BLAST | 17 | |
Motifi | 362 – 364 | Nuclear localization signalSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 279 – 300 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 317 – 334 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 362 – 381 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 553 – 567 | Acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 586 – 612 | Pro residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 391 – 413 | C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 484 – 507 | C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QSFC, Eukaryota |
GeneTreei | ENSGT00530000063777 |
HOGENOMi | CLU_024193_0_0_1 |
InParanoidi | Q9ULX6 |
OMAi | SGNMRFG |
OrthoDBi | 902224at2759 |
PhylomeDBi | Q9ULX6 |
TreeFami | TF105407 |
Family and domain databases
InterProi | View protein in InterPro IPR007071, AKAP95 IPR034736, ZF_C2H2_AKAP95 |
PANTHERi | PTHR12190, PTHR12190, 1 hit |
Pfami | View protein in Pfam PF04988, AKAP95, 1 hit |
PROSITEi | View protein in PROSITE PS51799, ZF_C2H2_AKAP95, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSYTGFVQGS ETTLQSTYSD TSAQPTCDYG YGTWNSGTNR GYEGYGYGYG
60 70 80 90 100
YGQDNTTNYG YGMATSHSWE MPSSDTNANT SASGSASADS VLSRINQRLD
110 120 130 140 150
MVPHLETDMM QGGVYGSGGE RYDSYESCDS RAVLSERDLY RSGYDYSELD
160 170 180 190 200
PEMEMAYEGQ YDAYRDQFRM RGNDTFGPRA QGWARDARSG RPMASGYGRM
210 220 230 240 250
WEDPMGARGQ CMSGASRLPS LFSQNIIPEY GMFQGMRGGG AFPGGSRFGF
260 270 280 290 300
GFGNGMKQMR RTWKTWTTAD FRTKKKKRKQ GGSPDEPDSK ATRTDCSDNS
310 320 330 340 350
DSDNDEGTEG EATEGLEGTE AVEKGSRVDG EDEEGKEDGR EEGKEDPEKG
360 370 380 390 400
ALTTQDENGQ TKRKLQAGKK SQDKQKKRQR DRMVERIQFV CSLCKYRTFY
410 420 430 440 450
EDEMASHLDS KFHKEHFKYV GTKLPKQTAD FLQEYVTNKT KKTEELRKTV
460 470 480 490 500
EDLDGLIQQI YRDQDLTQEI AMEHFVKKVE AAHCAACDLF IPMQFGIIQK
510 520 530 540 550
HLKTMDHNRN RRLMMEQSKK SSLMVARSIL NNKLISKKLE RYLKGENPFT
560 570 580 590 600
DSPEEEKEQE EAEGGALDEG AQGEAAGISE GAEGVPAQPP VPPEPAPGAV
610 620 630 640
SPPPPPPPEE EEEGAVPLLG GALQRQIRGI PGLDVEDDEE GGGGAP
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A7P0TAE3 | A0A7P0TAE3_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 612 | Annotation score: | ||
A0A7P0TAJ5 | A0A7P0TAJ5_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 536 | Annotation score: | ||
V9GZ50 | V9GZ50_HUMAN | A kinase (PRKA) anchor protein 8-li... | AKAP8L hCG_36782 | 431 | Annotation score: | ||
A0A7P0T847 | A0A7P0T847_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 395 | Annotation score: | ||
A0A7P0T9N5 | A0A7P0T9N5_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 444 | Annotation score: | ||
A0A7P0TAD3 | A0A7P0TAD3_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 465 | Annotation score: | ||
A0A7P0TBP0 | A0A7P0TBP0_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 408 | Annotation score: | ||
M0R1Y5 | M0R1Y5_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 109 | Annotation score: | ||
M0QYT7 | M0QYT7_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 162 | Annotation score: | ||
M0R010 | M0R010_HUMAN | A-kinase anchor protein 8-like | AKAP8L | 161 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | D → N in AAF86048 (PubMed:10748171).Curated | 1 | |
Sequence conflicti | 189 | S → N in AAF86048 (PubMed:10748171).Curated | 1 | |
Sequence conflicti | 351 – 358 | ALTTQDEN → EFSWGAWC in BAA34791 (PubMed:9853615).Curated | 8 | |
Sequence conflicti | 599 – 616 | AVSPP…EEGAV → GRVAATAAAPRRRRRRAPW in AAF86048 (PubMed:10748171).CuratedAdd BLAST | 18 | |
Sequence conflicti | 641 – 646 | GGGGAP → EGRRGRPV in AAF86048 (PubMed:10748171).Curated | 6 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_068822 | 458 | Q → H1 PublicationCorresponds to variant dbSNP:rs2058322Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044426 | 61 – 121 | Missing in isoform 2. 1 PublicationAdd BLAST | 61 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025905 mRNA Translation: BAA85003.1 AJ243467 mRNA Translation: CAB65092.1 AF199414 mRNA Translation: AAF86048.1 Frameshift. AK295956 mRNA Translation: BAG58736.1 AB451326 mRNA Translation: BAG70140.1 AB451469 mRNA Translation: BAG70283.1 AC005785 Genomic DNA No translation available. AC006128 Genomic DNA No translation available. KF456508 Genomic DNA No translation available. KF456511 Genomic DNA No translation available. CH471106 Genomic DNA Translation: EAW84475.1 BC000713 mRNA Translation: AAH00713.1 AB015332 mRNA Translation: BAA34791.1 Different initiation. |
CCDSi | CCDS46005.1 [Q9ULX6-1] CCDS77249.1 [Q9ULX6-2] |
RefSeqi | NP_001278407.1, NM_001291478.1 [Q9ULX6-2] NP_055186.3, NM_014371.3 [Q9ULX6-1] |
Genome annotation databases
Ensembli | ENST00000397410; ENSP00000380557; ENSG00000011243 ENST00000595465; ENSP00000470952; ENSG00000011243 [Q9ULX6-2] |
GeneIDi | 26993 |
KEGGi | hsa:26993 |
MANE-Selecti | ENST00000397410.10; ENSP00000380557.3; NM_014371.4; NP_055186.3 |
UCSCi | uc002naw.2, human [Q9ULX6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025905 mRNA Translation: BAA85003.1 AJ243467 mRNA Translation: CAB65092.1 AF199414 mRNA Translation: AAF86048.1 Frameshift. AK295956 mRNA Translation: BAG58736.1 AB451326 mRNA Translation: BAG70140.1 AB451469 mRNA Translation: BAG70283.1 AC005785 Genomic DNA No translation available. AC006128 Genomic DNA No translation available. KF456508 Genomic DNA No translation available. KF456511 Genomic DNA No translation available. CH471106 Genomic DNA Translation: EAW84475.1 BC000713 mRNA Translation: AAH00713.1 AB015332 mRNA Translation: BAA34791.1 Different initiation. |
CCDSi | CCDS46005.1 [Q9ULX6-1] CCDS77249.1 [Q9ULX6-2] |
RefSeqi | NP_001278407.1, NM_001291478.1 [Q9ULX6-2] NP_055186.3, NM_014371.3 [Q9ULX6-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 117940, 160 interactors |
CORUMi | Q9ULX6 |
DIPi | DIP-27541N |
IntActi | Q9ULX6, 157 interactors |
MINTi | Q9ULX6 |
STRINGi | 9606.ENSP00000380557 |
PTM databases
GlyGeni | Q9ULX6, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9ULX6 |
PhosphoSitePlusi | Q9ULX6 |
Genetic variation databases
BioMutai | AKAP8L |
DMDMi | 296439446 |
Proteomic databases
EPDi | Q9ULX6 |
jPOSTi | Q9ULX6 |
MassIVEi | Q9ULX6 |
MaxQBi | Q9ULX6 |
PaxDbi | Q9ULX6 |
PeptideAtlasi | Q9ULX6 |
PRIDEi | Q9ULX6 |
ProteomicsDBi | 85148 [Q9ULX6-1] |
Protocols and materials databases
Antibodypediai | 7497, 197 antibodies from 26 providers |
DNASUi | 26993 |
Genome annotation databases
Ensembli | ENST00000397410; ENSP00000380557; ENSG00000011243 ENST00000595465; ENSP00000470952; ENSG00000011243 [Q9ULX6-2] |
GeneIDi | 26993 |
KEGGi | hsa:26993 |
MANE-Selecti | ENST00000397410.10; ENSP00000380557.3; NM_014371.4; NP_055186.3 |
UCSCi | uc002naw.2, human [Q9ULX6-1] |
Organism-specific databases
CTDi | 26993 |
DisGeNETi | 26993 |
GeneCardsi | AKAP8L |
HGNCi | HGNC:29857, AKAP8L |
HPAi | ENSG00000011243, Low tissue specificity |
MIMi | 609475, gene |
neXtProti | NX_Q9ULX6 |
OpenTargetsi | ENSG00000011243 |
PharmGKBi | PA134867364 |
VEuPathDBi | HostDB:ENSG00000011243 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSFC, Eukaryota |
GeneTreei | ENSGT00530000063777 |
HOGENOMi | CLU_024193_0_0_1 |
InParanoidi | Q9ULX6 |
OMAi | SGNMRFG |
OrthoDBi | 902224at2759 |
PhylomeDBi | Q9ULX6 |
TreeFami | TF105407 |
Enzyme and pathway databases
PathwayCommonsi | Q9ULX6 |
SignaLinki | Q9ULX6 |
SIGNORi | Q9ULX6 |
Miscellaneous databases
BioGRID-ORCSi | 26993, 13 hits in 1054 CRISPR screens |
ChiTaRSi | AKAP8L, human |
GeneWikii | AKAP8L |
GenomeRNAii | 26993 |
Pharosi | Q9ULX6, Tbio |
PROi | PR:Q9ULX6 |
RNActi | Q9ULX6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000011243, Expressed in left lobe of thyroid gland and 230 other tissues |
ExpressionAtlasi | Q9ULX6, baseline and differential |
Genevisiblei | Q9ULX6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007071, AKAP95 IPR034736, ZF_C2H2_AKAP95 |
PANTHERi | PTHR12190, PTHR12190, 1 hit |
Pfami | View protein in Pfam PF04988, AKAP95, 1 hit |
PROSITEi | View protein in PROSITE PS51799, ZF_C2H2_AKAP95, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | AKP8L_HUMAN | |
Accessioni | Q9ULX6Primary (citable) accession number: Q9ULX6 Secondary accession number(s): B4DJ74 Q9UGM0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | September 12, 2018 | |
Last modified: | February 23, 2022 | |
This is version 197 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families