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Protein

Transcription factor-like 5 protein

Gene

TCFL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.1 Publication

Miscellaneous

Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune disease caused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 and R3 epitopes, which are recognized by T- and B-cells, respectively.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ9UL49
SIGNORiQ9UL49

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor-like 5 protein
Alternative name(s):
Cha transcription factor
HPV-16 E2-binding protein 1
Short name:
E2BP-1
Gene namesi
Name:TCFL5
Synonyms:CHA, E2BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101190.12
HGNCiHGNC:11646 TCFL5
MIMi604745 gene
neXtProtiNX_Q9UL49

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10732
OpenTargetsiENSG00000101190
PharmGKBiPA36398

Polymorphism and mutation databases

BioMutaiTCFL5
DMDMi166214983

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274751 – 500Transcription factor-like 5 proteinAdd BLAST500

Proteomic databases

MaxQBiQ9UL49
PaxDbiQ9UL49
PeptideAtlasiQ9UL49
PRIDEiQ9UL49
ProteomicsDBi84948
84949 [Q9UL49-1]
84950 [Q9UL49-2]

PTM databases

iPTMnetiQ9UL49
PhosphoSitePlusiQ9UL49

Expressioni

Tissue specificityi

Isoform 3 is testis specific. Isoform 2 is pancreas specific.1 Publication

Developmental stagei

Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.1 Publication

Gene expression databases

BgeeiENSG00000101190 Expressed in 215 organ(s), highest expression level in testis
CleanExiHS_TCFL5
ExpressionAtlasiQ9UL49 baseline and differential
GenevisibleiQ9UL49 HS

Organism-specific databases

HPAiHPA055223

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115955, 2 interactors
STRINGi9606.ENSP00000334294

Structurei

3D structure databases

ProteinModelPortaliQ9UL49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini400 – 450bHLHPROSITE-ProRule annotationAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni347 – 356R3 epitope (recognized by Chagas's antibodies)10
Regioni481 – 500R1 epitope (recognized by Chagas's antibodies)Add BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 182Ala-richAdd BLAST116

Phylogenomic databases

eggNOGiENOG410IECS Eukaryota
ENOG410YEAA LUCA
GeneTreeiENSGT00390000002821
HOGENOMiHOG000231268
HOVERGENiHBG067309
InParanoidiQ9UL49
KOiK09092
OMAiDRFNSIQ
OrthoDBiEOG091G0G81
PhylomeDBiQ9UL49
TreeFamiTF336112

Family and domain databases

Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR039583 TCFL5
PANTHERiPTHR15402 PTHR15402, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 3 (identifier: Q9UL49-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGPGPREPP PEAGAAGGEA AVEGAGGGDA ALGEPGLSFT TTDLSLVEMT
60 70 80 90 100
EVEYTQLQHI LCSHMEAAAD GELETRLNSA LLAAAGPGAG AGGFAAGGQG
110 120 130 140 150
GAAPVYPVLC PSALAADAPC LGHIDFQELR MMLLSEAGAA EKTSGGGDGA
160 170 180 190 200
RARADGAAKE GAGAAAAAAG PDGAPEARAK PAVRVRLEDR FNSIPAEPPP
210 220 230 240 250
APRGPEPPEP GGALNNLVTL IRHPSELMNV PLQQQNKCTA LVKNKTAATT
260 270 280 290 300
TALQFTYPLF TTNACSTSGN SNLSQTQSSS NSCSVLEAAK HQDIGLPRAF
310 320 330 340 350
SFCYQQEIES TKQTLGSRNK VLPEQVWIKV GEAALCKQAL KRNRSRMRQL
360 370 380 390 400
DTNVERRALG EIQNVGEGAT ATQGAWQSSE SSQANLGEQA QSGPQGGRSQ
410 420 430 440 450
RRERHNRMER DRRRRIRICC DELNLLVPFC NAETDKATTL QWTTAFLKYI
460 470 480 490 500
QERHGDSLKK EFESVFCGKT GRRLKLTRPD SLVTCPAQGS LQSSPSMEIK
Length:500
Mass (Da):52,697
Last modified:January 15, 2008 - v2
Checksum:i39918DCA21A94E7B
GO
Isoform 1 (identifier: Q9UL49-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Show »
Length:452
Mass (Da):48,263
Checksum:iB501EF5BCE12B5A8
GO
Isoform 2 (identifier: Q9UL49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-227: Missing.

Show »
Length:273
Mass (Da):30,479
Checksum:iC2811B004570E76A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W9A4F8W9A4_HUMAN
Transcription factor-like 5 protein
TCFL5
483Annotation score:

Sequence cautioni

The sequence BAA36557 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75T → M in BAA36557 (PubMed:9763657).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061263272N → D. Corresponds to variant dbSNP:rs17854409Ensembl.1
Natural variantiVAR_049555380E → D. Corresponds to variant dbSNP:rs34304654Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0021601 – 227Missing in isoform 2. 1 PublicationAdd BLAST227
Alternative sequenceiVSP_0303101 – 48Missing in isoform 1. CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271337 mRNA Translation: CAC24700.1
AF070992 mRNA Translation: AAD53986.1
AL035669 Genomic DNA No translation available.
AB012124 mRNA Translation: BAA36557.1 Different initiation.
CCDSiCCDS13506.1 [Q9UL49-3]
RefSeqiNP_006593.2, NM_006602.3 [Q9UL49-3]
UniGeneiHs.126248

Genome annotation databases

EnsembliENST00000335351; ENSP00000334294; ENSG00000101190 [Q9UL49-3]
GeneIDi10732
KEGGihsa:10732
UCSCiuc002ydp.3 human [Q9UL49-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271337 mRNA Translation: CAC24700.1
AF070992 mRNA Translation: AAD53986.1
AL035669 Genomic DNA No translation available.
AB012124 mRNA Translation: BAA36557.1 Different initiation.
CCDSiCCDS13506.1 [Q9UL49-3]
RefSeqiNP_006593.2, NM_006602.3 [Q9UL49-3]
UniGeneiHs.126248

3D structure databases

ProteinModelPortaliQ9UL49
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115955, 2 interactors
STRINGi9606.ENSP00000334294

PTM databases

iPTMnetiQ9UL49
PhosphoSitePlusiQ9UL49

Polymorphism and mutation databases

BioMutaiTCFL5
DMDMi166214983

Proteomic databases

MaxQBiQ9UL49
PaxDbiQ9UL49
PeptideAtlasiQ9UL49
PRIDEiQ9UL49
ProteomicsDBi84948
84949 [Q9UL49-1]
84950 [Q9UL49-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335351; ENSP00000334294; ENSG00000101190 [Q9UL49-3]
GeneIDi10732
KEGGihsa:10732
UCSCiuc002ydp.3 human [Q9UL49-3]

Organism-specific databases

CTDi10732
DisGeNETi10732
EuPathDBiHostDB:ENSG00000101190.12
GeneCardsiTCFL5
H-InvDBiHIX0203065
HGNCiHGNC:11646 TCFL5
HPAiHPA055223
MIMi604745 gene
neXtProtiNX_Q9UL49
OpenTargetsiENSG00000101190
PharmGKBiPA36398
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IECS Eukaryota
ENOG410YEAA LUCA
GeneTreeiENSGT00390000002821
HOGENOMiHOG000231268
HOVERGENiHBG067309
InParanoidiQ9UL49
KOiK09092
OMAiDRFNSIQ
OrthoDBiEOG091G0G81
PhylomeDBiQ9UL49
TreeFamiTF336112

Enzyme and pathway databases

SignaLinkiQ9UL49
SIGNORiQ9UL49

Miscellaneous databases

ChiTaRSiTCFL5 human
GenomeRNAii10732
PROiPR:Q9UL49
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101190 Expressed in 215 organ(s), highest expression level in testis
CleanExiHS_TCFL5
ExpressionAtlasiQ9UL49 baseline and differential
GenevisibleiQ9UL49 HS

Family and domain databases

Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR039583 TCFL5
PANTHERiPTHR15402 PTHR15402, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTCFL5_HUMAN
AccessioniPrimary (citable) accession number: Q9UL49
Secondary accession number(s): O94771, Q9BYW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: January 15, 2008
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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