UniProtKB - Q9UL19 (PLAT4_HUMAN)
Phospholipase A and acyltransferase 4
PLAAT4
Functioni
Exhibits both phospholipase A1/2 and acyltransferase activities (PubMed:19615464, PubMed:22605381, PubMed:22825852, PubMed:26503625).
Shows phospholipase A1 (PLA1) and A2 (PLA2), catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (PubMed:19615464, PubMed:22605381, PubMed:22825852).
For most substrates, PLA1 activity is much higher than PLA2 activity (PubMed:19615464).
Shows O-acyltransferase activity, catalyzing the transfer of a fatty acyl group from glycerophospholipid to the hydroxyl group of lysophospholipid (PubMed:19615464).
Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs) (PubMed:19615464, PubMed:22605381, PubMed:22825852).
Promotes keratinocyte differentiation via activation of TGM1 (PubMed:17762858).
1 Publication4 PublicationsCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+3 PublicationsEC:3.1.1.43 PublicationsThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+3 PublicationsEC:3.1.1.323 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 2-hexadecanoyl-sn-glycero-3-phosphocholine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = 2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphoethanolamine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexanoyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + H+ + hexanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine + 1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine = 1,2-diheptadecanoyl-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine + 1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine = 1,2-diheptadecanoyl-sn-glycero-3-phospho-N-(9Z-octadecenoyl)-ethanolamine + 2-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=400 µM for dipalmitoyl-PC1 Publication
- Vmax=530 nmol/min/mg enzyme with dipalmitoyl-PC as substrate1 Publication
- Vmax=240 nmol/min/mg enzyme with dipalmitoyl-PE as substrate1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 23 | PROSITE-ProRule annotation | 1 | |
Active sitei | 35 | PROSITE-ProRule annotation | 1 | |
Active sitei | 113 | Acyl-thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 1-acyl-2-lysophosphatidylserine acylhydrolase activity Source: UniProtKB-EC
- acyltransferase activity Source: Reactome
- N-acyltransferase activity Source: UniProtKB
- phosphatidylserine 1-acylhydrolase activity Source: UniProtKB-EC
- phospholipase A1 activity Source: UniProtKB
- phospholipase A2 activity Source: UniProtKB
GO - Biological processi
- lipid catabolic process Source: UniProtKB-KW
- N-acylphosphatidylethanolamine metabolic process Source: UniProtKB
- negative regulation of cell population proliferation Source: ProtInc
- phosphatidylethanolamine acyl-chain remodeling Source: Reactome
- phospholipid metabolic process Source: UniProtKB
- positive regulation of keratinocyte differentiation Source: UniProtKB
- positive regulation of protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Transferase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | Q9UL19 |
Reactomei | R-HSA-1482839, Acyl chain remodelling of PE |
SignaLinki | Q9UL19 |
Chemistry databases
SwissLipidsi | SLP:000001076 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipase A and acyltransferase 4Imported (EC:2.3.1.-3 Publications, EC:3.1.1.323 Publications, EC:3.1.1.43 Publications)Alternative name(s): HRAS-like suppressor 4 Short name: HRSL4 RAR-responsive protein TIG3 Retinoic acid receptor responder protein 3 Retinoid-inducible gene 1 protein Tazarotene-induced gene 3 protein |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9869, PLAAT4 |
MIMi | 605092, gene |
neXtProti | NX_Q9UL19 |
VEuPathDBi | HostDB:ENSG00000133321 |
Subcellular locationi
Other locations
- Membrane 1 Publication; Single-pass membrane protein Sequence analysis
Cytosol
- cytosol Source: Reactome
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 134 | CytoplasmicSequence analysisAdd BLAST | 134 | |
Transmembranei | 135 – 155 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 156 – 164 | LumenalSequence analysis | 9 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 5920 |
OpenTargetsi | ENSG00000133321 |
PharmGKBi | PA34230 |
Miscellaneous databases
Pharosi | Q9UL19, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4630864 |
Genetic variation databases
BioMutai | RARRES3 |
DMDMi | 20140910 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152490 | 1 – 164 | Phospholipase A and acyltransferase 4Add BLAST | 164 |
Proteomic databases
MassIVEi | Q9UL19 |
PaxDbi | Q9UL19 |
PeptideAtlasi | Q9UL19 |
PRIDEi | Q9UL19 |
ProteomicsDBi | 84932 [Q9UL19-1] 84933 [Q9UL19-2] |
PTM databases
iPTMneti | Q9UL19 |
PhosphoSitePlusi | Q9UL19 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000133321, Expressed in epithelium of bronchus and 239 other tissues |
ExpressionAtlasi | Q9UL19, baseline and differential |
Genevisiblei | Q9UL19, HS |
Organism-specific databases
HPAi | ENSG00000133321, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TGM1.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 111855, 26 interactors |
IntActi | Q9UL19, 2 interactors |
STRINGi | 9606.ENSP00000255688 |
Chemistry databases
BindingDBi | Q9UL19 |
Miscellaneous databases
RNActi | Q9UL19, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9UL19 |
SMRi | Q9UL19 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 129 | LRATPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | Essential for its ability regulate keratinocyte differentiation1 PublicationAdd BLAST | 40 | |
Regioni | 124 – 164 | Interaction with TGM11 PublicationAdd BLAST | 41 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S0JN, Eukaryota |
GeneTreei | ENSGT00940000162878 |
HOGENOMi | CLU_109418_0_1_1 |
InParanoidi | Q9UL19 |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q9UL19 |
TreeFami | TF330836 |
Family and domain databases
InterProi | View protein in InterPro IPR007053, LRAT_dom IPR033210, PLAAT4 |
PANTHERi | PTHR13943:SF36, PTHR13943:SF36, 1 hit |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASPHQEPKP GDLIEIFRLG YEHWALYIGD GYVIHLAPPS EYPGAGSSSV
60 70 80 90 100
FSVLSNSAEV KRERLEDVVG GCCYRVNNSL DHEYQPRPVE VIISSAKEMV
110 120 130 140 150
GQKMKYSIVS RNCEHFVTQL RYGKSRCKQV EKAKVEVGVA TALGILVVAG
160
CSFAIRRYQK KATA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA6NG31 | A6NG31_HUMAN | Phospholipase A and acyltransferase... | PLAAT4 | 164 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 44 | G → R in BAG56873 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 63 | E → G in AAC84000 (PubMed:9843971).Curated | 1 | |
Sequence conflicti | 118 | T → A in AAC84000 (PubMed:9843971).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049480 | 69 | V → L. Corresponds to variant dbSNP:rs35502888Ensembl. | 1 | |
Natural variantiVAR_049481 | 162 | A → V. Corresponds to variant dbSNP:rs35845275Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041496 | 131 – 164 | EKAKV…KKATA → RTSLDNPSPSAWGWTHGAVQ PGDHCE in isoform 2. 1 PublicationAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060228 mRNA Translation: AAC84000.1 AF092922 mRNA Translation: AAF02294.1 AB453252 mRNA Translation: BAH22446.1 AB030815 mRNA Translation: BAB08109.1 AK293357 mRNA Translation: BAG56873.1 AK312110 mRNA Translation: BAG35046.1 AP001591 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74156.1 BC009678 mRNA Translation: AAH09678.1 |
CCDSi | CCDS41662.1 [Q9UL19-1] |
RefSeqi | NP_004576.2, NM_004585.4 [Q9UL19-1] |
Genome annotation databases
Ensembli | ENST00000255688; ENSP00000255688; ENSG00000133321 ENST00000439013; ENSP00000402943; ENSG00000133321 [Q9UL19-2] |
GeneIDi | 5920 |
KEGGi | hsa:5920 |
MANE-Selecti | ENST00000255688.8; ENSP00000255688.3; NM_004585.5; NP_004576.2 |
UCSCi | uc001nxf.5, human [Q9UL19-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060228 mRNA Translation: AAC84000.1 AF092922 mRNA Translation: AAF02294.1 AB453252 mRNA Translation: BAH22446.1 AB030815 mRNA Translation: BAB08109.1 AK293357 mRNA Translation: BAG56873.1 AK312110 mRNA Translation: BAG35046.1 AP001591 Genomic DNA No translation available. CH471076 Genomic DNA Translation: EAW74156.1 BC009678 mRNA Translation: AAH09678.1 |
CCDSi | CCDS41662.1 [Q9UL19-1] |
RefSeqi | NP_004576.2, NM_004585.4 [Q9UL19-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LKT | NMR | - | A | 1-125 | [»] | |
2MY9 | NMR | - | A | 1-125 | [»] | |
BMRBi | Q9UL19 | |||||
SMRi | Q9UL19 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111855, 26 interactors |
IntActi | Q9UL19, 2 interactors |
STRINGi | 9606.ENSP00000255688 |
Chemistry databases
BindingDBi | Q9UL19 |
ChEMBLi | CHEMBL4630864 |
SwissLipidsi | SLP:000001076 |
PTM databases
iPTMneti | Q9UL19 |
PhosphoSitePlusi | Q9UL19 |
Genetic variation databases
BioMutai | RARRES3 |
DMDMi | 20140910 |
Proteomic databases
MassIVEi | Q9UL19 |
PaxDbi | Q9UL19 |
PeptideAtlasi | Q9UL19 |
PRIDEi | Q9UL19 |
ProteomicsDBi | 84932 [Q9UL19-1] 84933 [Q9UL19-2] |
Protocols and materials databases
Antibodypediai | 2443, 239 antibodies from 35 providers |
DNASUi | 5920 |
Genome annotation databases
Ensembli | ENST00000255688; ENSP00000255688; ENSG00000133321 ENST00000439013; ENSP00000402943; ENSG00000133321 [Q9UL19-2] |
GeneIDi | 5920 |
KEGGi | hsa:5920 |
MANE-Selecti | ENST00000255688.8; ENSP00000255688.3; NM_004585.5; NP_004576.2 |
UCSCi | uc001nxf.5, human [Q9UL19-1] |
Organism-specific databases
CTDi | 5920 |
DisGeNETi | 5920 |
GeneCardsi | PLAAT4 |
HGNCi | HGNC:9869, PLAAT4 |
HPAi | ENSG00000133321, Low tissue specificity |
MIMi | 605092, gene |
neXtProti | NX_Q9UL19 |
OpenTargetsi | ENSG00000133321 |
PharmGKBi | PA34230 |
VEuPathDBi | HostDB:ENSG00000133321 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S0JN, Eukaryota |
GeneTreei | ENSGT00940000162878 |
HOGENOMi | CLU_109418_0_1_1 |
InParanoidi | Q9UL19 |
OrthoDBi | 1602481at2759 |
PhylomeDBi | Q9UL19 |
TreeFami | TF330836 |
Enzyme and pathway databases
PathwayCommonsi | Q9UL19 |
Reactomei | R-HSA-1482839, Acyl chain remodelling of PE |
SignaLinki | Q9UL19 |
Miscellaneous databases
BioGRID-ORCSi | 5920, 9 hits in 1044 CRISPR screens |
ChiTaRSi | RARRES3, human |
GeneWikii | RARRES3 |
GenomeRNAii | 5920 |
Pharosi | Q9UL19, Tbio |
PROi | PR:Q9UL19 |
RNActi | Q9UL19, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000133321, Expressed in epithelium of bronchus and 239 other tissues |
ExpressionAtlasi | Q9UL19, baseline and differential |
Genevisiblei | Q9UL19, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007053, LRAT_dom IPR033210, PLAAT4 |
PANTHERi | PTHR13943:SF36, PTHR13943:SF36, 1 hit |
Pfami | View protein in Pfam PF04970, LRAT, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLAT4_HUMAN | |
Accessioni | Q9UL19Primary (citable) accession number: Q9UL19 Secondary accession number(s): B2R599 O95200 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families