UniProtKB - Q9UKX7 (NUP50_HUMAN)
Nuclear pore complex protein Nup50
NUP50
Functioni
GO - Biological processi
- intracellular transport of virus Source: Reactome
- mRNA export from nucleus Source: Reactome
- protein import into nucleus Source: GO_Central
- protein sumoylation Source: Reactome
- regulation of cellular response to heat Source: Reactome
- regulation of gene silencing by miRNA Source: Reactome
- regulation of glycolytic process Source: Reactome
- tRNA export from nucleus Source: Reactome
- viral life cycle Source: Reactome
- viral process Source: Reactome
- viral transcription Source: Reactome
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9UKX7 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-6784531, tRNA processing in the nucleus R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events |
SIGNORi | Q9UKX7 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear pore complex protein Nup50Alternative name(s): 50 kDa nucleoporin Nuclear pore-associated protein 60 kDa-like Nucleoporin Nup50 |
Gene namesi | Name:NUP50 Synonyms:NPAP60L ORF Names:PRO1146 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8065, NUP50 |
MIMi | 604646, gene |
neXtProti | NX_Q9UKX7 |
VEuPathDBi | HostDB:ENSG00000093000.18 |
Subcellular locationi
Nucleus
- nuclear pore complex 1 Publication
- Nucleus membrane By similarity; Peripheral membrane protein By similarity; Nucleoplasmic side By similarity
Note: Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity). Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065). Associates with the newly formed nuclear membrane during telophase (PubMed:12802065). In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity).By similarity1 Publication
Nucleus
- nuclear membrane Source: UniProtKB
- nuclear pore Source: ProtInc
- nucleoplasm Source: HPA
Other locations
- host cell Source: GOC
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10762 |
OpenTargetsi | ENSG00000093000 |
PharmGKBi | PA31852 |
Miscellaneous databases
Pharosi | Q9UKX7, Tbio |
Genetic variation databases
BioMutai | NUP50 |
DMDMi | 20455193 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000204868 | 1 – 468 | Nuclear pore complex protein Nup50Add BLAST | 468 | ||
Isoform 2 (identifier: Q9UKX7-2) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 8 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 52 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 83 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 127 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 208 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 221 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 234 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 246 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 259 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 270 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 296 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 353 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 450 | N6-acetyllysineBy similarity | 1 | ||
Isoform 2 (identifier: Q9UKX7-2) | |||||
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9UKX7 |
jPOSTi | Q9UKX7 |
MassIVEi | Q9UKX7 |
PaxDbi | Q9UKX7 |
PeptideAtlasi | Q9UKX7 |
PRIDEi | Q9UKX7 |
ProteomicsDBi | 84906 [Q9UKX7-1] 84907 [Q9UKX7-2] |
PTM databases
iPTMneti | Q9UKX7 |
PhosphoSitePlusi | Q9UKX7 |
SwissPalmi | Q9UKX7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000093000, Expressed in testis and 243 other tissues |
ExpressionAtlasi | Q9UKX7, baseline and differential |
Genevisiblei | Q9UKX7, HS |
Organism-specific databases
HPAi | ENSG00000093000, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Does not interact with TPR.
By similarityBinary interactionsi
Hide detailsQ9UKX7
Protein-protein interaction databases
BioGRIDi | 115982, 130 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
IntActi | Q9UKX7, 67 interactors |
MINTi | Q9UKX7 |
STRINGi | 9606.ENSP00000345895 |
Miscellaneous databases
RNActi | Q9UKX7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UKX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UKX7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 76 – 77 | 1 | 2 | |
Repeati | 113 – 114 | 2 | 2 | |
Repeati | 225 – 226 | 3 | 2 | |
Repeati | 273 – 274 | 4 | 2 | |
Repeati | 303 – 304 | 5 | 2 | |
Domaini | 335 – 468 | RanBD1PROSITE-ProRule annotationAdd BLAST | 134 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 76 – 304 | 5 X 2 AA repeats of F-GAdd BLAST | 229 | |
Regioni | 144 – 206 | Binding to CDKN1BBy similarityAdd BLAST | 63 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 55 – 91 | Gly-richAdd BLAST | 37 | |
Compositional biasi | 268 – 330 | Ser-richAdd BLAST | 63 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2724, Eukaryota |
GeneTreei | ENSGT00440000035348 |
HOGENOMi | CLU_032593_0_0_1 |
InParanoidi | Q9UKX7 |
OMAi | GSAYHKQ |
OrthoDBi | 793797at2759 |
PhylomeDBi | Q9UKX7 |
TreeFami | TF106504 |
Family and domain databases
DisProti | DP02455 |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR015007, NUP2/50/61 IPR011993, PH-like_dom_sf IPR000156, Ran_bind_dom |
Pfami | View protein in Pfam PF08911, NUP50, 1 hit PF00638, Ran_BP1, 1 hit |
SMARTi | View protein in SMART SM00160, RanBD, 1 hit |
PROSITEi | View protein in PROSITE PS50196, RANBD1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF
60 70 80 90 100
ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF
110 120 130 140 150
ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA
160 170 180 190 200
CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ
210 220 230 240 250
HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK
260 270 280 290 300
MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS
310 320 330 340 350
SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT
360 370 380 390 400
EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN
410 420 430 440 450
LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK
460
TSEDADELHK ILLEKKDA
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AHA5 | B1AHA5_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 76 | Annotation score: | ||
B0QY21 | B0QY21_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 178 | Annotation score: | ||
X6RKQ2 | X6RKQ2_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 45 | Annotation score: | ||
F2Z3L9 | F2Z3L9_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 30 | Annotation score: | ||
A0A0G2JI20 | A0A0G2JI20_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 8 | Annotation score: | ||
A0A0G2JIF9 | A0A0G2JIF9_HUMAN | Nuclear pore complex protein Nup50 | NUP50 | 10 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 235 | T → S in AAD53401 (PubMed:10449902).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040633 | 1 – 28 | Missing in isoform 2. 1 PublicationAdd BLAST | 28 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF107840 mRNA Translation: AAD53401.1 AF116624 mRNA Translation: AAF71047.1 CR456533 mRNA Translation: CAG30419.1 AK314454 mRNA Translation: BAG37062.1 AL008718 Genomic DNA No translation available. Z82243 Genomic DNA No translation available. CH471138 Genomic DNA Translation: EAW73370.1 CH471138 Genomic DNA Translation: EAW73372.1 BC016055 mRNA Translation: AAH16055.1 BC028125 mRNA Translation: AAH28125.1 BC070133 mRNA Translation: AAH70133.1 AL389949 mRNA Translation: CAB97527.1 AL389950 mRNA Translation: CAB97528.1 |
CCDSi | CCDS14062.1 [Q9UKX7-1] CCDS14063.1 [Q9UKX7-2] |
RefSeqi | NP_009103.2, NM_007172.3 [Q9UKX7-1] NP_705931.1, NM_153645.2 [Q9UKX7-2] XP_005261369.1, XM_005261312.1 [Q9UKX7-2] XP_005261371.1, XM_005261314.1 [Q9UKX7-2] XP_006724166.1, XM_006724103.1 [Q9UKX7-1] XP_006724167.1, XM_006724104.1 [Q9UKX7-2] XP_011528135.1, XM_011529833.1 [Q9UKX7-1] XP_016884026.1, XM_017028537.1 [Q9UKX7-2] |
Genome annotation databases
Ensembli | ENST00000347635; ENSP00000345895; ENSG00000093000 [Q9UKX7-1] ENST00000396096; ENSP00000379403; ENSG00000093000 [Q9UKX7-2] ENST00000407019; ENSP00000385555; ENSG00000093000 [Q9UKX7-2] |
GeneIDi | 10762 |
KEGGi | hsa:10762 |
UCSCi | uc003bfr.4, human [Q9UKX7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF107840 mRNA Translation: AAD53401.1 AF116624 mRNA Translation: AAF71047.1 CR456533 mRNA Translation: CAG30419.1 AK314454 mRNA Translation: BAG37062.1 AL008718 Genomic DNA No translation available. Z82243 Genomic DNA No translation available. CH471138 Genomic DNA Translation: EAW73370.1 CH471138 Genomic DNA Translation: EAW73372.1 BC016055 mRNA Translation: AAH16055.1 BC028125 mRNA Translation: AAH28125.1 BC070133 mRNA Translation: AAH70133.1 AL389949 mRNA Translation: CAB97527.1 AL389950 mRNA Translation: CAB97528.1 |
CCDSi | CCDS14062.1 [Q9UKX7-1] CCDS14063.1 [Q9UKX7-2] |
RefSeqi | NP_009103.2, NM_007172.3 [Q9UKX7-1] NP_705931.1, NM_153645.2 [Q9UKX7-2] XP_005261369.1, XM_005261312.1 [Q9UKX7-2] XP_005261371.1, XM_005261314.1 [Q9UKX7-2] XP_006724166.1, XM_006724103.1 [Q9UKX7-1] XP_006724167.1, XM_006724104.1 [Q9UKX7-2] XP_011528135.1, XM_011529833.1 [Q9UKX7-1] XP_016884026.1, XM_017028537.1 [Q9UKX7-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EC1 | NMR | - | A | 351-468 | [»] | |
3TJ3 | X-ray | 2.70 | C/D | 1-109 | [»] | |
SMRi | Q9UKX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115982, 130 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
IntActi | Q9UKX7, 67 interactors |
MINTi | Q9UKX7 |
STRINGi | 9606.ENSP00000345895 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q9UKX7 |
PhosphoSitePlusi | Q9UKX7 |
SwissPalmi | Q9UKX7 |
Genetic variation databases
BioMutai | NUP50 |
DMDMi | 20455193 |
Proteomic databases
EPDi | Q9UKX7 |
jPOSTi | Q9UKX7 |
MassIVEi | Q9UKX7 |
PaxDbi | Q9UKX7 |
PeptideAtlasi | Q9UKX7 |
PRIDEi | Q9UKX7 |
ProteomicsDBi | 84906 [Q9UKX7-1] 84907 [Q9UKX7-2] |
Protocols and materials databases
Antibodypediai | 27795, 358 antibodies |
DNASUi | 10762 |
Genome annotation databases
Ensembli | ENST00000347635; ENSP00000345895; ENSG00000093000 [Q9UKX7-1] ENST00000396096; ENSP00000379403; ENSG00000093000 [Q9UKX7-2] ENST00000407019; ENSP00000385555; ENSG00000093000 [Q9UKX7-2] |
GeneIDi | 10762 |
KEGGi | hsa:10762 |
UCSCi | uc003bfr.4, human [Q9UKX7-1] |
Organism-specific databases
CTDi | 10762 |
DisGeNETi | 10762 |
GeneCardsi | NUP50 |
HGNCi | HGNC:8065, NUP50 |
HPAi | ENSG00000093000, Low tissue specificity |
MIMi | 604646, gene |
neXtProti | NX_Q9UKX7 |
OpenTargetsi | ENSG00000093000 |
PharmGKBi | PA31852 |
VEuPathDBi | HostDB:ENSG00000093000.18 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2724, Eukaryota |
GeneTreei | ENSGT00440000035348 |
HOGENOMi | CLU_032593_0_0_1 |
InParanoidi | Q9UKX7 |
OMAi | GSAYHKQ |
OrthoDBi | 793797at2759 |
PhylomeDBi | Q9UKX7 |
TreeFami | TF106504 |
Enzyme and pathway databases
PathwayCommonsi | Q9UKX7 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-6784531, tRNA processing in the nucleus R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events |
SIGNORi | Q9UKX7 |
Miscellaneous databases
BioGRID-ORCSi | 10762, 286 hits in 968 CRISPR screens |
ChiTaRSi | NUP50, human |
EvolutionaryTracei | Q9UKX7 |
GeneWikii | NUP50 |
GenomeRNAii | 10762 |
Pharosi | Q9UKX7, Tbio |
PROi | PR:Q9UKX7 |
RNActi | Q9UKX7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000093000, Expressed in testis and 243 other tissues |
ExpressionAtlasi | Q9UKX7, baseline and differential |
Genevisiblei | Q9UKX7, HS |
Family and domain databases
DisProti | DP02455 |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR015007, NUP2/50/61 IPR011993, PH-like_dom_sf IPR000156, Ran_bind_dom |
Pfami | View protein in Pfam PF08911, NUP50, 1 hit PF00638, Ran_BP1, 1 hit |
SMARTi | View protein in SMART SM00160, RanBD, 1 hit |
PROSITEi | View protein in PROSITE PS50196, RANBD1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUP50_HUMAN | |
Accessioni | Q9UKX7Primary (citable) accession number: Q9UKX7 Secondary accession number(s): B1AHA4 Q9P1K5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | May 2, 2002 | |
Last modified: | April 7, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references