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Protein

Nuclear pore complex protein Nup50

Gene

NUP50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-159227 Transport of the SLBP independent Mature mRNA
R-HSA-159230 Transport of the SLBP Dependant Mature mRNA
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-HSA-180746 Nuclear import of Rev protein
R-HSA-180910 Vpr-mediated nuclear import of PICs
R-HSA-191859 snRNP Assembly
R-HSA-3108214 SUMOylation of DNA damage response and repair proteins
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-6784531 tRNA processing in the nucleus
SIGNORiQ9UKX7

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:NUP50
Synonyms:NPAP60L
ORF Names:PRO1146
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000093000.18
HGNCiHGNC:8065 NUP50
MIMi604646 gene
neXtProtiNX_Q9UKX7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10762
OpenTargetsiENSG00000093000
PharmGKBiPA31852

Polymorphism and mutation databases

BioMutaiNUP50
DMDMi20455193

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048681 – 468Nuclear pore complex protein Nup50Add BLAST468
Isoform 2 (identifier: Q9UKX7-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei52PhosphoserineCombined sources1
Modified residuei83N6-acetyllysineCombined sources1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei208PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei234PhosphoserineBy similarity1
Modified residuei246PhosphothreonineCombined sources1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei296PhosphoserineCombined sources1
Cross-linki353Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei450N6-acetyllysineBy similarity1
Isoform 2 (identifier: Q9UKX7-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UKX7
PaxDbiQ9UKX7
PeptideAtlasiQ9UKX7
PRIDEiQ9UKX7
ProteomicsDBi84906
84907 [Q9UKX7-2]

PTM databases

iPTMnetiQ9UKX7
PhosphoSitePlusiQ9UKX7

Miscellaneous databases

PMAP-CutDBiQ9UKX7

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

Gene expression databases

BgeeiENSG00000093000
CleanExiHS_NUP50
ExpressionAtlasiQ9UKX7 baseline and differential
GenevisibleiQ9UKX7 HS

Organism-specific databases

HPAiHPA047162
HPA048328

Interactioni

Subunit structurei

Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Does not interact with TPR.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115982, 101 interactors
IntActiQ9UKX7, 35 interactors
MINTiQ9UKX7
STRINGi9606.ENSP00000345895

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Beta strandi15 – 17Combined sources3
Helixi32 – 37Combined sources6
Beta strandi358 – 368Combined sources11
Beta strandi370 – 385Combined sources16
Beta strandi387 – 401Combined sources15
Turni420 – 422Combined sources3
Beta strandi423 – 428Combined sources6
Beta strandi436 – 441Combined sources6
Beta strandi443 – 448Combined sources6
Helixi452 – 467Combined sources16

3D structure databases

ProteinModelPortaliQ9UKX7
SMRiQ9UKX7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKX7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati113 – 11422
Repeati225 – 22632
Repeati273 – 27442
Repeati303 – 30452
Domaini335 – 468RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3045 X 2 AA repeats of F-GAdd BLAST229
Regioni144 – 206Binding to CDKN1BBy similarityAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 91Gly-richAdd BLAST37
Compositional biasi268 – 330Ser-richAdd BLAST63

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724 Eukaryota
ENOG410YPHB LUCA
GeneTreeiENSGT00440000035348
HOVERGENiHBG052697
InParanoidiQ9UKX7
KOiK14295
OMAiTFLFHGN
OrthoDBiEOG091G0HML
PhylomeDBiQ9UKX7
TreeFamiTF106504

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR015007 NUP2/50/61
IPR011993 PH-like_dom_sf
IPR000156 Ran_bind_dom
PfamiView protein in Pfam
PF08911 NUP50, 1 hit
PF00638 Ran_BP1, 1 hit
SMARTiView protein in SMART
SM00160 RanBD, 1 hit
PROSITEiView protein in PROSITE
PS50196 RANBD1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UKX7-1) [UniParc]FASTAAdd to basket
Also known as: Npap60L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF
60 70 80 90 100
ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF
110 120 130 140 150
ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA
160 170 180 190 200
CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ
210 220 230 240 250
HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK
260 270 280 290 300
MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS
310 320 330 340 350
SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT
360 370 380 390 400
EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN
410 420 430 440 450
LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK
460
TSEDADELHK ILLEKKDA
Length:468
Mass (Da):50,144
Last modified:May 2, 2002 - v2
Checksum:i1F2775AE9AC8FAC4
GO
Isoform 2 (identifier: Q9UKX7-2) [UniParc]FASTAAdd to basket
Also known as: Npap60S

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: Contrarily to Npap60L, Npap60S does not displaces NLSs, but stabilizes their binding to importin-alpha.Combined sources
Show »
Length:440
Mass (Da):46,863
Checksum:iC78B5EF4366680E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235T → S in AAD53401 (PubMed:10449902).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0406331 – 28Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA Translation: AAD53401.1
AF116624 mRNA Translation: AAF71047.1
CR456533 mRNA Translation: CAG30419.1
AK314454 mRNA Translation: BAG37062.1
AL008718 Genomic DNA No translation available.
Z82243 Genomic DNA No translation available.
CH471138 Genomic DNA Translation: EAW73370.1
CH471138 Genomic DNA Translation: EAW73372.1
BC016055 mRNA Translation: AAH16055.1
BC028125 mRNA Translation: AAH28125.1
BC070133 mRNA Translation: AAH70133.1
AL389949 mRNA Translation: CAB97527.1
AL389950 mRNA Translation: CAB97528.1
CCDSiCCDS14062.1 [Q9UKX7-1]
CCDS14063.1 [Q9UKX7-2]
RefSeqiNP_009103.2, NM_007172.3 [Q9UKX7-1]
NP_705931.1, NM_153645.2 [Q9UKX7-2]
XP_005261369.1, XM_005261312.1 [Q9UKX7-2]
XP_005261371.1, XM_005261314.1 [Q9UKX7-2]
XP_006724166.1, XM_006724103.1 [Q9UKX7-1]
XP_006724167.1, XM_006724104.1 [Q9UKX7-2]
XP_011528135.1, XM_011529833.1 [Q9UKX7-1]
XP_016884026.1, XM_017028537.1 [Q9UKX7-2]
UniGeneiHs.475103

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000 [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000 [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000 [Q9UKX7-2]
GeneIDi10762
KEGGihsa:10762
UCSCiuc003bfr.4 human [Q9UKX7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUP50_HUMAN
AccessioniPrimary (citable) accession number: Q9UKX7
Secondary accession number(s): B1AHA4
, B2RB15, O75644, Q8N6V5, Q9NPM9, Q9NPR6, Q9P1K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: July 18, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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