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UniProtKB - Q9UKX7 (NUP50_HUMAN)

Protein

Nuclear pore complex protein Nup50

Gene

NUP50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).By similarity1 Publication

GO - Molecular functioni

  • Ran GTPase binding Source: GO_Central
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-159227 Transport of the SLBP independent Mature mRNA
R-HSA-159230 Transport of the SLBP Dependant Mature mRNA
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-HSA-180746 Nuclear import of Rev protein
R-HSA-180910 Vpr-mediated nuclear import of PICs
R-HSA-191859 snRNP Assembly
R-HSA-3108214 SUMOylation of DNA damage response and repair proteins
R-HSA-3232142 SUMOylation of ubiquitinylation proteins
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-4085377 SUMOylation of SUMOylation proteins
R-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-6784531 tRNA processing in the nucleus
SIGNORiQ9UKX7

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear pore complex protein Nup50
Alternative name(s):
50 kDa nucleoporin
Nuclear pore-associated protein 60 kDa-like
Nucleoporin Nup50
Gene namesi
Name:NUP50
Synonyms:NPAP60L
ORF Names:PRO1146
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000093000.18
HGNCiHGNC:8065 NUP50
MIMi604646 gene
neXtProtiNX_Q9UKX7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10762
OpenTargetsiENSG00000093000
PharmGKBiPA31852

Polymorphism and mutation databases

BioMutaiNUP50
DMDMi20455193

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048681 – 468Nuclear pore complex protein Nup50Add BLAST468
Isoform 2 (identifier: Q9UKX7-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei52PhosphoserineCombined sources1
Modified residuei83N6-acetyllysineCombined sources1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei208PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei234PhosphoserineBy similarity1
Modified residuei246PhosphothreonineCombined sources1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei296PhosphoserineCombined sources1
Cross-linki353Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei450N6-acetyllysineBy similarity1
Isoform 2 (identifier: Q9UKX7-2)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UKX7
PaxDbiQ9UKX7
PeptideAtlasiQ9UKX7
PRIDEiQ9UKX7
ProteomicsDBi84906
84907 [Q9UKX7-2]

PTM databases

iPTMnetiQ9UKX7
PhosphoSitePlusiQ9UKX7

Miscellaneous databases

PMAP-CutDBiQ9UKX7

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

Gene expression databases

BgeeiENSG00000093000 Expressed in 234 organ(s), highest expression level in testis
CleanExiHS_NUP50
ExpressionAtlasiQ9UKX7 baseline and differential
GenevisibleiQ9UKX7 HS

Organism-specific databases

HPAiHPA047162
HPA048328

Interactioni

Subunit structurei

Interacts with Importin alpha-2, Importin beta, Importin beta-2, NUP153, Ran binding protein 7, CDKN1B and itself (By similarity). Does not interact with TPR.By similarity

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi115982, 101 interactors
IntActiQ9UKX7, 29 interactors
MINTiQ9UKX7
STRINGi9606.ENSP00000345895

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UKX7
SMRiQ9UKX7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UKX7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati76 – 7712
Repeati113 – 11422
Repeati225 – 22632
Repeati273 – 27442
Repeati303 – 30452
Domaini335 – 468RanBD1PROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 3045 X 2 AA repeats of F-GAdd BLAST229
Regioni144 – 206Binding to CDKN1BBy similarityAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 91Gly-richAdd BLAST37
Compositional biasi268 – 330Ser-richAdd BLAST63

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2724 Eukaryota
ENOG410YPHB LUCA
GeneTreeiENSGT00440000035348
HOVERGENiHBG052697
InParanoidiQ9UKX7
KOiK14295
OMAiCIPNPPL
OrthoDBiEOG091G0HML
PhylomeDBiQ9UKX7
TreeFamiTF106504

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR015007 NUP2/50/61
IPR011993 PH-like_dom_sf
IPR000156 Ran_bind_dom
PfamiView protein in Pfam
PF08911 NUP50, 1 hit
PF00638 Ran_BP1, 1 hit
SMARTiView protein in SMART
SM00160 RanBD, 1 hit
PROSITEiView protein in PROSITE
PS50196 RANBD1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UKX7-1) [UniParc]FASTAAdd to basket
Also known as: Npap60L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKRNAEKEL TDRNWDQEDE AEEVGTFSMA SEEVLKNRAI KKAKRRNVGF 
        60         70         80         90        100
ESDTGGAFKG FKGLVVPSGG GRFSGFGSGA GGKPLEGLSN GNNITSAPPF 
       110        120        130        140        150
ASAKAAADPK VAFGSLAANG PTTLVDKVSN PKTNGDSQQP SSSGLASSKA 
       160        170        180        190        200
CVGNAYHKQL AALNCSVRDW IVKHVNTNPL CDLTPIFKDY EKYLANIEQQ 
       210        220        230        240        250
HGNSGRNSES ESNKVAAETQ SPSLFGSTKL QQESTFLFHG NKTEDTPDKK 
       260        270        280        290        300
MEVASEKKTD PSSLGATSAS FNFGKKVDSS VLGSLSSVPL TGFSFSPGNS 
       310        320        330        340        350
SLFGKDTTQS KPVSSPFPTK PLEGQAEGDS GECKGGDEEE NDEPPKVVVT 
       360        370        380        390        400
EVKEEDAFYS KKCKLFYKKD NEFKEKGIGT LHLKPTANQK TQLLVRADTN 
       410        420        430        440        450
LGNILLNVLI PPNMPCTRTG KNNVLIVCVP NPPIDEKNAT MPVTMLIRVK 
       460 
TSEDADELHK ILLEKKDA
Length:468
Mass (Da):50,144
Last modified:May 2, 2002 - v2
Checksum:i1F2775AE9AC8FAC4
GO
Isoform 2 (identifier: Q9UKX7-2) [UniParc]FASTAAdd to basket
Also known as: Npap60S

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Note: Contrarily to Npap60L, Npap60S does not displaces NLSs, but stabilizes their binding to importin-alpha.Combined sources
Show »
Length:440
Mass (Da):46,863
Checksum:iC78B5EF4366680E2
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AHA5B1AHA5_HUMAN
Nuclear pore complex protein Nup50
NUP50
76Annotation score:
B0QY21B0QY21_HUMAN
Nuclear pore complex protein Nup50
NUP50
178Annotation score:
X6RKQ2X6RKQ2_HUMAN
Nuclear pore complex protein Nup50
NUP50
45Annotation score:
F2Z3L9F2Z3L9_HUMAN
Nuclear pore complex protein Nup50
NUP50
30Annotation score:
A0A0G2JI20A0A0G2JI20_HUMAN
Nuclear pore complex protein Nup50
NUP50
8Annotation score:
A0A0G2JIF9A0A0G2JIF9_HUMAN
Nuclear pore complex protein Nup50
NUP50
10Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti235T → S in AAD53401 (PubMed:10449902).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0406331 – 28Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA Translation: AAD53401.1
AF116624 mRNA Translation: AAF71047.1
CR456533 mRNA Translation: CAG30419.1
AK314454 mRNA Translation: BAG37062.1
AL008718 Genomic DNA No translation available.
Z82243 Genomic DNA No translation available.
CH471138 Genomic DNA Translation: EAW73370.1
CH471138 Genomic DNA Translation: EAW73372.1
BC016055 mRNA Translation: AAH16055.1
BC028125 mRNA Translation: AAH28125.1
BC070133 mRNA Translation: AAH70133.1
AL389949 mRNA Translation: CAB97527.1
AL389950 mRNA Translation: CAB97528.1
CCDSiCCDS14062.1 [Q9UKX7-1]
CCDS14063.1 [Q9UKX7-2]
RefSeqiNP_009103.2, NM_007172.3 [Q9UKX7-1]
NP_705931.1, NM_153645.2 [Q9UKX7-2]
XP_005261369.1, XM_005261312.1 [Q9UKX7-2]
XP_005261371.1, XM_005261314.1 [Q9UKX7-2]
XP_006724166.1, XM_006724103.1 [Q9UKX7-1]
XP_006724167.1, XM_006724104.1 [Q9UKX7-2]
XP_011528135.1, XM_011529833.1 [Q9UKX7-1]
XP_016884026.1, XM_017028537.1 [Q9UKX7-2]
UniGeneiHs.475103

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000 [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000 [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000 [Q9UKX7-2]
GeneIDi10762
KEGGihsa:10762
UCSCiuc003bfr.4 human [Q9UKX7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107840 mRNA Translation: AAD53401.1
AF116624 mRNA Translation: AAF71047.1
CR456533 mRNA Translation: CAG30419.1
AK314454 mRNA Translation: BAG37062.1
AL008718 Genomic DNA No translation available.
Z82243 Genomic DNA No translation available.
CH471138 Genomic DNA Translation: EAW73370.1
CH471138 Genomic DNA Translation: EAW73372.1
BC016055 mRNA Translation: AAH16055.1
BC028125 mRNA Translation: AAH28125.1
BC070133 mRNA Translation: AAH70133.1
AL389949 mRNA Translation: CAB97527.1
AL389950 mRNA Translation: CAB97528.1
CCDSiCCDS14062.1 [Q9UKX7-1]
CCDS14063.1 [Q9UKX7-2]
RefSeqiNP_009103.2, NM_007172.3 [Q9UKX7-1]
NP_705931.1, NM_153645.2 [Q9UKX7-2]
XP_005261369.1, XM_005261312.1 [Q9UKX7-2]
XP_005261371.1, XM_005261314.1 [Q9UKX7-2]
XP_006724166.1, XM_006724103.1 [Q9UKX7-1]
XP_006724167.1, XM_006724104.1 [Q9UKX7-2]
XP_011528135.1, XM_011529833.1 [Q9UKX7-1]
XP_016884026.1, XM_017028537.1 [Q9UKX7-2]
UniGeneiHs.475103

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EC1NMR-A351-468[»]
3TJ3X-ray2.70C/D1-109[»]
ProteinModelPortaliQ9UKX7
SMRiQ9UKX7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115982, 101 interactors
IntActiQ9UKX7, 29 interactors
MINTiQ9UKX7
STRINGi9606.ENSP00000345895

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiQ9UKX7
PhosphoSitePlusiQ9UKX7

Polymorphism and mutation databases

BioMutaiNUP50
DMDMi20455193

Proteomic databases

EPDiQ9UKX7
PaxDbiQ9UKX7
PeptideAtlasiQ9UKX7
PRIDEiQ9UKX7
ProteomicsDBi84906
84907 [Q9UKX7-2]

Protocols and materials databases

DNASUi10762
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000347635; ENSP00000345895; ENSG00000093000 [Q9UKX7-1]
ENST00000396096; ENSP00000379403; ENSG00000093000 [Q9UKX7-2]
ENST00000407019; ENSP00000385555; ENSG00000093000 [Q9UKX7-2]
GeneIDi10762
KEGGihsa:10762
UCSCiuc003bfr.4 human [Q9UKX7-1]

Organism-specific databases

CTDi10762
DisGeNETi10762
EuPathDBiHostDB:ENSG00000093000.18
GeneCardsiNUP50
HGNCiHGNC:8065 NUP50
HPAiHPA047162
HPA048328
MIMi604646 gene
neXtProtiNX_Q9UKX7
OpenTargetsiENSG00000093000
PharmGKBiPA31852
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2724 Eukaryota
ENOG410YPHB LUCA
GeneTreeiENSGT00440000035348
HOVERGENiHBG052697
InParanoidiQ9UKX7
KOiK14295
OMAiCIPNPPL
OrthoDBiEOG091G0HML
PhylomeDBiQ9UKX7
TreeFamiTF106504

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-159227 Transport of the SLBP independent Mature mRNA
R-HSA-159230 Transport of the SLBP Dependant Mature mRNA
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-HSA-180746 Nuclear import of Rev protein
R-HSA-180910 Vpr-mediated nuclear import of PICs
R-HSA-191859 snRNP Assembly
R-HSA-3108214 SUMOylation of DNA damage response and repair proteins
R-HSA-3232142 SUMOylation of ubiquitinylation proteins
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-4085377 SUMOylation of SUMOylation proteins
R-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-6784531 tRNA processing in the nucleus
SIGNORiQ9UKX7

Miscellaneous databases

EvolutionaryTraceiQ9UKX7
GeneWikiiNUP50
GenomeRNAii10762
PMAP-CutDBiQ9UKX7
PROiPR:Q9UKX7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000093000 Expressed in 234 organ(s), highest expression level in testis
CleanExiHS_NUP50
ExpressionAtlasiQ9UKX7 baseline and differential
GenevisibleiQ9UKX7 HS

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR015007 NUP2/50/61
IPR011993 PH-like_dom_sf
IPR000156 Ran_bind_dom
PfamiView protein in Pfam
PF08911 NUP50, 1 hit
PF00638 Ran_BP1, 1 hit
SMARTiView protein in SMART
SM00160 RanBD, 1 hit
PROSITEiView protein in PROSITE
PS50196 RANBD1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUP50_HUMAN
AccessioniPrimary (citable) accession number: Q9UKX7
Secondary accession number(s): B1AHA4
, B2RB15, O75644, Q8N6V5, Q9NPM9, Q9NPR6, Q9P1K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: November 7, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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