UniProtKB - Q9UKV5 (AMFR_HUMAN)

BLAST

>sp|Q9UKV5|AMFR_HUMAN E3 ubiquitin-protein ligase AMFR OS=Homo sapiens OX=9606 GN=AMFR PE=1 SV=2
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAP
ARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKD
KFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIH
LWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRY
LFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNH
FFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDL
QLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLD
LSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNK
SSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS

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History
Entry version 166 (07 Nov 2018)
Sequence version 2 (01 Jun 2003)
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Protein

E3 ubiquitin-protein ligase AMFR

Gene

AMFR

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.5 Publications

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	341 – 379	RING-typePROSITE-ProRule annotationAdd BLAST		39

GO - Molecular functionⁱ

BAT3 complex binding
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24424410
chaperone binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 21636303
- 23246001
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
metal ion binding Source: UniProtKB-KW
protein binding, bridging
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 24424410
signaling receptor activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 1649192
ubiquitin protein ligase activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24424410
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
ubiquitin-specific protease binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 24424410
ubiquitin-ubiquitin ligase activity
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24810856

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aging Source: Ensembl
endoplasmic reticulum mannose trimming Source: Reactome
endoplasmic reticulum unfolded protein response
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17157811
learning or memory Source: Ensembl
positive regulation of protein binding
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 24424410
protein autoubiquitination
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 24810856
protein complex oligomerization
Source: UniProtKB
Inferred from direct assayⁱ
- 17310145
protein folding Source: Reactome
protein K48-linked ubiquitination
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 12847084
- 24424410
protein polyubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 17310145
- 19103148
signal transduction
Source: ProtInc
Traceable author statementⁱ
- 1649192
ubiquitin-dependent ERAD pathway
Source: UniProtKB
Inferred from direct assayⁱ
- 11724934
- 17872946
ubiquitin-dependent protein catabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16168377
- 17043353

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Receptor, Transferase
Biological process	Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

BRENDAⁱ	2.3.2.B10 2681
Reactomeⁱ	R-HSA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle R-HSA-901032 ER Quality Control Compartment (ERQC)
SIGNORⁱ	Q9UKV5
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 ubiquitin-protein ligase AMFR (EC:2.3.2.272 Publications) Alternative name(s): Autocrine motility factor receptor Short name: AMF receptor RING finger protein 45 RING-type E3 ubiquitin transferase AMFRCurated gp78
Gene namesⁱ	Name:AMFR Synonyms:RNF45
Organismⁱ	Homo sapiens (Human)Imported
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 16

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000159461.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:463 AMFR
MIMⁱ	603243 gene
neXtProtⁱ	NX_Q9UKV5

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	82 – 102	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	122 – 142	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	145 – 165	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	186 – 206	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	215 – 235	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	276 – 296	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	429 – 449	HelicalSequence analysisAdd BLAST	21

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	341 – 344	CAIC → AAIA: Decreased palmitoylation. 1 Publication	4
Mutagenesisⁱ	356	C → A: Decreased palmitoylation. No degradation of HMGCR. 2 Publications	1
Mutagenesisⁱ	364	C → A: Decreased palmitoylation. 1 Publication	1
Mutagenesisⁱ	375 – 378	CPTC → APTA: Decreased palmitoylation. 1 Publication	4

Organism-specific databases

DisGeNET More...DisGeNETⁱ	267
Open Targets More...OpenTargetsⁱ	ENSG00000159461
PharmGKBⁱ	PA24768

Polymorphism and mutation databases

BioMutaⁱ	AMFR
DMDMⁱ	34922250

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000064579	1 – 643	E3 ubiquitin-protein ligase AMFRAdd BLAST		643

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	516	PhosphoserineCombined sources	1
Modified residueⁱ	523	PhosphoserineCombined sources	1
Modified residueⁱ	542	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Palmitoylation of the RING-type zing finger by ZDHHC6 promotes localization to the peripheral endoplasmic reticulum.1 Publication

Keywords - PTMⁱ

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDⁱ	Q9UKV5
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9UKV5
PaxDbⁱ	Q9UKV5
PeptideAtlas More...PeptideAtlasⁱ	Q9UKV5
PRIDEⁱ	Q9UKV5
ProteomicsDBⁱ	84893

PTM databases

iPTMnetⁱ	Q9UKV5
PhosphoSitePlusⁱ	Q9UKV5
SwissPalmⁱ	Q9UKV5

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000159461 Expressed in 221 organ(s), highest expression level in testis
CleanExⁱ	HS_AMFR
ExpressionAtlasⁱ	Q9UKV5 baseline and differential
Genevisibleⁱ	Q9UKV5 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB026381 HPA029018 HPA077835

HPAⁱ

CAB026381
HPA029018
HPA077835

Interactionⁱ

Subunit structureⁱ

Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation (By similarity). Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. Interacts with BAG6 (PubMed:21636303). Interacts with USP13 (via UBA 2 domain); the interaction is direct (PubMed:24424410).By similarity6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		11	EBI-1046367,EBI-1046367
ERLIN2	O94905	8	EBI-1046367,EBI-4400770
INSIG1	O15503	2	EBI-1046367,EBI-6252425
UBE2G2	P60604	19	EBI-1046367,EBI-1051028
Ube2g2	P60605	10	EBI-1046367,EBI-9108745	From Mus musculus.
VCP	P55072	10	EBI-1046367,EBI-355164

GO - Molecular functionⁱ

chaperone binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 21636303
- 23246001
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
protein binding, bridging
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 24424410
ubiquitin-specific protease binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 24424410

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	106764, 125 interactors
CORUMⁱ	Q9UKV5
Database of interacting proteins More...DIPⁱ	DIP-29060N
IntActⁱ	Q9UKV5, 32 interactors
Molecular INTeraction database More...MINTⁱ	Q9UKV5
STRINGⁱ	9606.ENSP00000290649

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	332 – 337	Combined sources	6
Beta strandⁱ	342 – 344	Combined sources	3
Beta strandⁱ	351 – 353	Combined sources	3
Beta strandⁱ	355 – 357	Combined sources	3
Beta strandⁱ	359 – 361	Combined sources	3
Helixⁱ	362 – 369	Combined sources	8
Beta strandⁱ	376 – 378	Combined sources	3
Helixⁱ	456 – 467	Combined sources	12
Helixⁱ	473 – 483	Combined sources	11
Helixⁱ	486 – 494	Combined sources	9
Helixⁱ	575 – 599	Combined sources	25
Helixⁱ	625 – 637	Combined sources	13

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9UKV5
SMRⁱ	Q9UKV5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9UKV5

EvolutionaryTraceⁱ

Q9UKV5

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	456 – 498	CUEPROSITE-ProRule annotationAdd BLAST		43

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	622 – 640	VCP/p97-interacting motif (VIM)1 PublicationAdd BLAST		19

Domainⁱ

The VCP/p97-interacting motif (VIM) is sufficient for binding VCP/p97 to form a complex capable of transferring VCP/p97 from the cytosol to microsomes.

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	341 – 379	RING-typePROSITE-ProRule annotationAdd BLAST		39

Keywords - Domainⁱ

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0802 Eukaryota COG5243 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062938
HOGENOMⁱ	HOG000172151
HOVERGENⁱ	HBG044694
InParanoidⁱ	Q9UKV5
KEGG Orthology (KO) More...KOⁱ	K10636
OMAⁱ	MWCLWFS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06NX
PhylomeDBⁱ	Q9UKV5
TreeFamⁱ	TF320052

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR003892 CUE IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02845 CUE, 1 hit PF13639 zf-RING_2, 1 hit
SMARTⁱ	View protein in SMART SM00546 CUE, 1 hit SM00184 RING, 1 hit
PROSITEⁱ	View protein in PROSITE PS51140 CUE, 1 hit PS50089 ZF_RING_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show all Align All

Q9UKV5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPLLFLERFP WPSLRTYTGL SGLALLGTII SAYRALSQPE AGPGEPDQLT 
        60         70         80         90        100
ASLQPEPPAP ARPSAGGPRA RDVAQYLLSD SLFVWVLVNT ACCVLMLVAK 
       110        120        130        140        150
LIQCIVFGPL RVSERQHLKD KFWNFIFYKF IFIFGVLNVQ TVEEVVMWCL 
       160        170        180        190        200
WFAGLVFLHL MVQLCKDRFE YLSFSPTTPM SSHGRVLSLL VAMLLSCCGL 
       210        220        230        240        250
AAVCSITGYT HGMHTLAFMA AESLLVTVRT AHVILRYVIH LWDLNHEGTW 
       260        270        280        290        300
EGKGTYVYYT DFVMELTLLS LDLMHHIHML LFGNIWLSMA SLVIFMQLRY 
       310        320        330        340        350
LFHEVQRRIR RHKNYLRVVG NMEARFAVAT PEELAVNNDD CAICWDSMQA 
       360        370        380        390        400
ARKLPCGHLF HNSCLRSWLE QDTSCPTCRM SLNIADNNRV REEHQGENLD 
       410        420        430        440        450
ENLVPVAAAE GRPRLNQHNH FFHFDGSRIA SWLPSFSVEV MHTTNILGIT 
       460        470        480        490        500
QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL QLTRSVEITT DNILEGRIQV 
       510        520        530        540        550
PFPTQRSDSI RPALNSPVER PSSDQEEGET SAQTERVPLD LSPRLEETLD 
       560        570        580        590        600
FGEVEVEPSE VEDFEARGSR FSKSADERQR MLVQRKDELL QQARKRFLNK 
       610        620        630        640 
SSEDDAASES FLPSEGASSD PVTLRRRMLA AAAERRLQKQ QTS

Length:643

Mass (Da):72,996

Last modified:June 1, 2003 - v2

Checksum:ⁱ8782324609C0E62A

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

R4GNG2	R4GNG2_HUMAN	E3 ubiquitin-protein ligase AMFR E3 ubiquitin-protein ligase AMFR	AMFR	299	Annotation score:
H3BSK3	H3BSK3_HUMAN	E3 ubiquitin-protein ligase AMFR E3 ubiquitin-protein ligase AMFR	AMFR	229	Annotation score:
H3BQM3	H3BQM3_HUMAN	E3 ubiquitin-protein ligase AMFR E3 ubiquitin-protein ligase AMFR	AMFR	165	Annotation score:
H3BRK9	H3BRK9_HUMAN	E3 ubiquitin-protein ligase AMFR E3 ubiquitin-protein ligase AMFR	AMFR	288	Annotation score:

Sequence cautionⁱ

The sequence AAA36671 differs from that shown. Several sequencing errors.Curated

The sequence AAA79362 differs from that shown. Reason: Frameshift at positions 355, 388, 411, 487, 537, 583 and 632.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	406	V → D in AAA79362 (PubMed:7626106).Curated	1
Sequence conflictⁱ	491	D → V in AAA79362 (PubMed:7626106).Curated	1
Sequence conflictⁱ	500	V → L in AAA79362 (PubMed:7626106).Curated	1
Sequence conflictⁱ	614	S → L in AAD56722 (PubMed:10456327).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_035790	605	D → V in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs373191257Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF124145 mRNA Translation: AAD56722.1 BC069197 mRNA Translation: AAH69197.1 L35233 mRNA Translation: AAA79362.1 Frameshift. M63175 mRNA Translation: AAA36671.1 Sequence problems.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10758.1
PIRⁱ	A39877
NCBI Reference Sequences More...RefSeqⁱ	NP_001135.3, NM_001144.5 NP_001310441.1, NM_001323512.1
UniGeneⁱ	Hs.295137

Genome annotation databases

Ensemblⁱ	ENST00000290649; ENSP00000290649; ENSG00000159461
GeneIDⁱ	267
KEGGⁱ	hsa:267
UCSC genome browser More...UCSCⁱ	uc002eiy.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9UKV5	AMFR isoform 1		PANTR		643	UniRef100_Q9UKV5
AMFR protein (Fragment)		HUMAN		292
Uncharacterized protein		PANPA		564
AMFR isoform 4 (Fragment)		PANTR		365
HCG1811773, isoform CRA_c		HUMAN		548
+2

Protein	Similar proteins		Species	Score	Length	Source
Q9UKV5	E3 ubiquitin-protein ligase AMFR		MOUSE		643	UniRef90_Q9UKV5
AMFR isoform 1		PANTR		643
AMFR protein (Fragment)		HUMAN		292
Uncharacterized protein		PANPA		564
Autocrine motility factor receptor		FELCA		781
+96

Protein	Similar proteins		Species	Score	Length	Source
Q9UKV5	E3 ubiquitin-protein ligase AMFR		MOUSE		643	UniRef50_Q9UKV5
AMFR isoform 1		PANTR		643
AMFR protein (Fragment)		HUMAN		292
Uncharacterized protein		PANPA		564
Autocrine motility factor receptor		FELCA		781
+102

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF124145 mRNA Translation: AAD56722.1 BC069197 mRNA Translation: AAH69197.1 L35233 mRNA Translation: AAA79362.1 Frameshift. M63175 mRNA Translation: AAA36671.1 Sequence problems.
CCDSⁱ	CCDS10758.1
PIRⁱ	A39877
RefSeqⁱ	NP_001135.3, NM_001144.5 NP_001310441.1, NM_001323512.1
UniGeneⁱ	Hs.295137

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2EJS	NMR	-	A	452-502	[»]
	2LVN	NMR	-	C	453-504	[»]
	2LVO	NMR	-	C	453-504	[»]
	2LVP	NMR	-	C	453-504	[»]
	2LVQ	NMR	-	D	453-504	[»]
	2LXH	NMR	-	C	313-393	[»]
	2LXP	NMR	-	B	574-600	[»]
				C	327-384	[»]
	3FSH	X-ray	2.76	C	574-601	[»]
	3H8K	X-ray	1.80	B	573-600	[»]
	3TIW	X-ray	1.80	C/D	622-640	[»]
	4G3O	X-ray	1.60	A	456-498	[»]
	4LAD	X-ray	2.30	B	313-393	[»]
				B	574-600	[»]
ProteinModelPortalⁱ	Q9UKV5
SMRⁱ	Q9UKV5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	106764, 125 interactors
CORUMⁱ	Q9UKV5
DIPⁱ	DIP-29060N
IntActⁱ	Q9UKV5, 32 interactors
MINTⁱ	Q9UKV5
STRINGⁱ	9606.ENSP00000290649

PTM databases

iPTMnetⁱ	Q9UKV5
PhosphoSitePlusⁱ	Q9UKV5
SwissPalmⁱ	Q9UKV5

Polymorphism and mutation databases

BioMutaⁱ	AMFR
DMDMⁱ	34922250

Proteomic databases

EPDⁱ	Q9UKV5
MaxQBⁱ	Q9UKV5
PaxDbⁱ	Q9UKV5
PeptideAtlasⁱ	Q9UKV5
PRIDEⁱ	Q9UKV5
ProteomicsDBⁱ	84893

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000290649; ENSP00000290649; ENSG00000159461
GeneIDⁱ	267
KEGGⁱ	hsa:267
UCSCⁱ	uc002eiy.4 human

Organism-specific databases

CTDⁱ	267
DisGeNETⁱ	267
EuPathDBⁱ	HostDB:ENSG00000159461.14
GeneCardsⁱ	AMFR
HGNCⁱ	HGNC:463 AMFR
HPAⁱ	CAB026381 HPA029018 HPA077835
MIMⁱ	603243 gene
neXtProtⁱ	NX_Q9UKV5
OpenTargetsⁱ	ENSG00000159461
PharmGKBⁱ	PA24768
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0802 Eukaryota COG5243 LUCA
GeneTreeⁱ	ENSGT00530000062938
HOGENOMⁱ	HOG000172151
HOVERGENⁱ	HBG044694
InParanoidⁱ	Q9UKV5
KOⁱ	K10636
OMAⁱ	MWCLWFS
OrthoDBⁱ	EOG091G06NX
PhylomeDBⁱ	Q9UKV5
TreeFamⁱ	TF320052

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
BRENDAⁱ	2.3.2.B10 2681
Reactomeⁱ	R-HSA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle R-HSA-901032 ER Quality Control Compartment (ERQC)
SIGNORⁱ	Q9UKV5

Miscellaneous databases

ChiTaRSⁱ	AMFR human
EvolutionaryTraceⁱ	Q9UKV5
GeneWikiⁱ	AMFR
GenomeRNAiⁱ	267
Protein Ontology More...PROⁱ	PR:Q9UKV5
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000159461 Expressed in 221 organ(s), highest expression level in testis
CleanExⁱ	HS_AMFR
ExpressionAtlasⁱ	Q9UKV5 baseline and differential
Genevisibleⁱ	Q9UKV5 HS

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR003892 CUE IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD
Pfamⁱ	View protein in Pfam PF02845 CUE, 1 hit PF13639 zf-RING_2, 1 hit
SMARTⁱ	View protein in SMART SM00546 CUE, 1 hit SM00184 RING, 1 hit
PROSITEⁱ	View protein in PROSITE PS51140 CUE, 1 hit PS50089 ZF_RING_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AMFR_HUMAN
Accessionⁱ	Q9UKV5Primary (citable) accession number: Q9UKV5 Secondary accession number(s): P26442, Q8IZ70
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
	Last sequence update:	June 1, 2003
	Last modified:	November 7, 2018
	This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9UKV5 (AMFR_HUMAN)

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E3 ubiquitin-protein ligase AMFR

AMFR

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Golgi apparatus

Nucleus

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ