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UniProtKB - Q9UKT4 (FBX5_HUMAN)

Entry version 173 (23 Feb 2022)
Sequence version 1 (01 May 2000)
Previous versions | rss
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Protein

F-box only protein 5

Gene

FBXO5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:17485488, PubMed:17234884, PubMed:17875940, PubMed:23708001, PubMed:23708605, PubMed:16921029).

During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:29875408, PubMed:17485488, PubMed:17234884, PubMed:17875940, PubMed:23708001, PubMed:23708605, PubMed:16921029).

During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed:29875408).

Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed:29875408).

As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed:23708605, PubMed:23708001, PubMed:16921029).

Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed:17234884, PubMed:17485488, PubMed:17875940).

During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity).

Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity).

Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed:29850565).

By similarity8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi378Zinc 1PROSITE-ProRule annotation1
Metal bindingi381Zinc 1PROSITE-ProRule annotation1
Metal bindingi396Zinc 1PROSITE-ProRule annotation1
Metal bindingi401Zinc 1PROSITE-ProRule annotation1
Metal bindingi406Zinc 2PROSITE-ProRule annotation1
Metal bindingi409Zinc 2PROSITE-ProRule annotation1
Metal bindingi414Zinc 2PROSITE-ProRule annotation1
Metal bindingi419Zinc 2PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri374 – 422ZBR-typePROSITE-ProRule annotationAdd BLAST49

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q9UKT4

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1
R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176417, Phosphorylation of Emi1
R-HSA-68881, Mitotic Metaphase/Anaphase Transition
R-HSA-69205, G1/S-Specific Transcription

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q9UKT4

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q9UKT4

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
F-box only protein 5Curated
Alternative name(s):
Early mitotic inhibitor 11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:FBXO5Imported
Synonyms:EMI11 Publication, FBX5Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:13584, FBXO5

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		606013, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q9UKT4

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000112029

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi143E → A: Delays degradation. 1 Publication1
Mutagenesisi144D → A: Does not affect protein stability. 1 Publication1
Mutagenesisi145S → A: Does not affect protein stability; when associated with A-149. 1 Publication1
Mutagenesisi145S → E: Degraded in similar manner to wild-type. 3 Publications1
Mutagenesisi145S → N: Not mitotically degraded. Shows impaired interaction with BTRC and reduced phosphate incorporation; when associated with N-149. 3 Publications1
Mutagenesisi146G → V: Does not affect protein stability. 1 Publication1
Mutagenesisi148S → A: Degraded in similar manner to wild-type. 1 Publication1
Mutagenesisi149S → A: Does not affect protein stability; when associated with A-145. 1 Publication1
Mutagenesisi149S → E: Degraded in similar manner to wild-type. 3 Publications1
Mutagenesisi149S → N: Not mitotically degraded. Shows impaired interaction with BTRC and reduced phosphate incorporation; when associated with N-145. 3 Publications1
Mutagenesisi182S → A: Shows impaired interaction with BTRC. 1 Publication1
Mutagenesisi210 – 216KRNPKVD → AAAAAAA: Loss of interaction with EVI5. 1 Publication7
Mutagenesisi322 – 325RTPL → ATPA: Does not affect inhibition of UBE2S-catalyzed chain elongation. Efficiently inhibits the degradation of PTTG1 at relatively high concentration. Reduces the competitive ability of FBXO5 to inhibit the association of PTTG1 to APC. Can not compete with the APC substrate for APC binding. Decreases inhibition of CCNB1 ubiquitination by UBE2C. 2 Publications4
Mutagenesisi339 – 345Missing : Impairs CCNB1 ubiquitination by UBE2C; when associated with 356-Y--R-358 del. 1 Publication7
Mutagenesisi345L → A: Substantially impairs inhibition of CCNB1 ubiquitination by UBE2C; when associated with 346-S--T-355 del. 1 Publication1
Mutagenesisi346 – 355Missing : Inhibits CCNB1 ubiquitination by UBE2C. Substantially impairs inhibition of CCNB1 ubiquitination by UBE2C; when associated with A-345; A-356 and A-358. 1 Publication10
Mutagenesisi356 – 358Missing : Impairs CCNB1 ubiquitination by UBE2C; when associated with 339-K--L-345 del. 1 Publication3
Mutagenesisi356Y → A: Substantially impairs inhibition of CCNB1 ubiquitination by UBE2C; when associated with 346-S--T-355 del. 1 Publication1
Mutagenesisi358R → A: Substantially impairs inhibition of CCNB1 ubiquitination by UBE2C; when associated with 346-S--T-355 del. 1 Publication1
Mutagenesisi375L → A: Decreases UBE2C-mediated ubiquitination. 1 Publication1
Mutagenesisi376K → A: Decreases UBE2C-mediated ubiquitination. 1 Publication1
Mutagenesisi393R → A: Decreases UBE2C-mediated ubiquitination. 1 Publication1
Mutagenesisi401C → S: Reduced inhibition of APC. Does not affect the FBXO5-mediated inhibitory activity against ubiquitin chain assembly. Does not affect the FBXO5-mediated inhibitory activity against ubiquitin chain assembly; when associated with S-401. Does not affect inhibition of UBE2S-catalyzed chain elongation. Reduces the competitive ability of FBXO5 to inhibit the association of securin to APC. Can still compete with the APC substrate for APC binding. Fails to inhibit ubiquitin chain assembly by UBE2C or mono-ubiquitination by UBE2D1. Largely abolishes the inhibitory activity against protein degradation. Fails to inactivate APC-FZR1 complex. Allows FBXO5 degradation in the absence of CDK4 inhibitor. 3 Publications1
Mutagenesisi406C → S: Does not affect the inhibitory activity against chain assembly; when associated with S-401. 1 Publication1
Mutagenesisi409C → A: Decreases UBE2C-mediated ubiquitination. 1 Publication1
Mutagenesisi444 – 445LR → AA: Loses inhibitory activity on UBE2S-catalyzed chain elongation. 1 Publication2

Organism-specific databases

DisGeNET

More...DisGeNETi		26271

Open Targets

More...OpenTargetsi		ENSG00000112029

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA28045

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q9UKT4, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		FBXO5

Domain mapping of disease mutations (DMDM)

More...DMDMi		24636847

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001198811 – 447F-box only protein 5Add BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei94PhosphoserineBy similarity1
Modified residuei102PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression (PubMed:12791267, PubMed:15469984). Phosphorylated by RPS6KA2; increases and stabilizes interaction with CDC20 (By similarity).By similarity2 Publications
Ubiquitinated by the SCF(BTRC) complex following phosphorylation by PLK1 (PubMed:15469984). Undergoes both 'Lys-11' and 'Lys-48'-linked polyubiquitination by APC-FZR1 complex leading to degradation by proteasome during G1 phase (PubMed:29875408). Degraded through the SCF(BTRC) complex; degradation occurs during oocyte maturation, between germinal vesicle breakdown (GVBD) and meiosis I, and is required for the meiosis I-meiosis II transition (By similarity).By similarity2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q9UKT4

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q9UKT4

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q9UKT4

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q9UKT4

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9UKT4

PeptideAtlas

More...PeptideAtlasi		Q9UKT4

PRoteomics IDEntifications database

More...PRIDEi		Q9UKT4

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		84847 [Q9UKT4-1]
84848 [Q9UKT4-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q9UKT4, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9UKT4

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9UKT4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis.1 Publication

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Up-regulated at 7 days after osteogenic induction (PubMed:29850565). Down-regulated in late G2 phase or mitosis (PubMed:17485488). Down-regulated in G2 phase after DNA damage in a CDKN1A-dependent manner (PubMed:19211842). Down-regulated in G1 phase when APC-FZR1 complex is active and accumulates at the G1-S transition, coincident with the inactivation of APC-FZR1 complex (PubMed:29875408). At the G1-S transition, transcriptionally induced by the E2F transcription factor (PubMed:11988738).5 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000112029, Expressed in ventricular zone and 184 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9UKT4, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000112029, Group enriched (bone marrow, lymphoid tissue)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).

Interacts with BTRC; mediates proteolysis by the SCF ubiquitin ligase complex leading to activation of APC in late mitosis and subsequent mitotic progression (PubMed:12791267).

Interacts with FZR1/CDH1 and the N-terminal substrate-binding domain of CDC20; prevents APC activation (PubMed:11988738).

Also interacts with EVI5 which blocks its phosphorylation by PLK1 and prevents its subsequent binding to BTRC and degradation (PubMed:16439210).

Interacts simultaneously with anaphase promoting complex (APC), through at least ANAPC2, CDC23, CDC27, the APC substrate GMNN and the APC activator FZR1 (PubMed:23708001, PubMed:26083744).

Interacts with UBE2S; interferes with the activity of UBE2S mainly by disrupting the dynamic electrostatic association between the C-terminal tail of UBE2S and ANAPC2 (PubMed:23708001).

Interacts with RPS6KA2; cooperates to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity).

By similarity5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		117655, 54 interactors

Database of interacting proteins

More...DIPi		DIP-38023N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...ELMi		Q9UKT4

Protein interaction database and analysis system

More...IntActi		Q9UKT4, 26 interactors

Molecular INTeraction database

More...MINTi		Q9UKT4

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000229758

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q9UKT4, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1447
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		Q9UKT4

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9UKT4

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini250 – 296F-boxAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni135 – 244Interaction with EVI51 PublicationAdd BLAST110
Regioni261 – 409Requires for efficient binding to CDC20By similarityAdd BLAST149
Regioni261 – 339Sufficient for interaction with RPS6KA2; Prevents association of CDC20 with RPS6KA2By similarityAdd BLAST79
Regioni305 – 447Inhibits APC ubiquitin ligase activity1 PublicationAdd BLAST143
Regioni322 – 325Competitively blocks access of APC substrates to the D-box coreceptor formed by FZR1 and ANAPC101 Publication4
Regioni337 – 358DisorderedSequence analysisAdd BLAST22
Regioni378 – 420Allows a rapid multiple mono-ubiquitination of the APC substrate, but strongly inhibits the slow ubiquitin chain elongation catalyzed by UBCH101 PublicationAdd BLAST43
Regioni437 – 447Sufficient to suppress UBE2S activity; essential for interaction with UBE2S; competitively inhibits the rapide ubiquitin chain elongation by UBE2D1 which blocks UBE2D1 with APC; indispensable for recruitment and position of FBXO5 to the catalytic site of APC; abrogates the inhibition of ubiquitin chain assembly primarily catalyzed by UBE2S; inhibits the ubiquitination by either UBE2C or UBE2D11 PublicationAdd BLAST11

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri374 – 422ZBR-typePROSITE-ProRule annotationAdd BLAST49

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QPWN, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00530000063692

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_055946_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9UKT4

Identification of Orthologs from Complete Genome Data

More...OMAi		IKFSPHA

Database of Orthologous Groups

More...OrthoDBi		521317at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9UKT4

TreeFam database of animal gene trees

More...TreeFami		TF101170

Family and domain databases

Database of protein disorder

More...DisProti		DP01450

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001810, F-box_dom
IPR044064, ZF_ZBR

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00646, F-box, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51872, ZF_ZBR, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9UKT4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSRRPCSCAL RPPRCSCSAS PSAVTAAGRP RPSDSCKEES STLSVKMKCD 
        60         70         80         90        100
FNCNHVHSGL KLVKPDDIGR LVSYTPAYLE GSCKDCIKDY ERLSCIGSPI 
       110        120        130        140        150
VSPRIVQLET ESKRLHNKEN QHVQQTLNST NEIEALETSR LYEDSGYSSF 
       160        170        180        190        200
SLQSGLSEHE EGSLLEENFG DSLQSCLLQI QSPDQYPNKN LLPVLHFEKV 
       210        220        230        240        250
VCSTLKKNAK RNPKVDREML KEIIARGNFR LQNIIGRKMG LECVDILSEL 
       260        270        280        290        300
FRRGLRHVLA TILAQLSDMD LINVSKVSTT WKKILEDDKG AFQLYSKAIQ 
       310        320        330        340        350
RVTENNNKFS PHASTREYVM FRTPLASVQK SAAQTSLKKD AQTKLSNQGD 
       360        370        380        390        400
QKGSTYSRHN EFSEVAKTLK KNESLKACIR CNSPAKYDCY LQRATCKREG 
       410        420        430        440 
CGFDYCTKCL CNYHTTKDCS DGKLLKASCK IGPLPGTKKS KKNLRRL
Length:447
Mass (Da):50,146
Last modified:May 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i196FBC2578F92120
GO
Isoform 2 (identifier: Q9UKT4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Show »
Length:401
Mass (Da):45,353
Checksum:i8E9E775625E62A4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti212N → D in BAG51487 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_024440107Q → E2 PublicationsCorresponds to variant dbSNP:rs2073260Ensembl.1
Natural variantiVAR_049038164L → F. Corresponds to variant dbSNP:rs7763565Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0413621 – 46Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF129535 mRNA Translation: AAF04469.1
AY079515 mRNA Translation: AAL86610.1
AK055221 mRNA Translation: BAG51487.1
AL080276 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47719.1
BC018905 mRNA Translation: AAH18905.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS47501.1 [Q9UKT4-2]
CCDS5242.1 [Q9UKT4-1]

NCBI Reference Sequences

More...RefSeqi		NP_001135994.1, NM_001142522.2 [Q9UKT4-2]
NP_036309.1, NM_012177.4 [Q9UKT4-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000229758; ENSP00000229758; ENSG00000112029
ENST00000367241; ENSP00000356210; ENSG00000112029 [Q9UKT4-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		26271

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:26271

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000229758.8; ENSP00000229758.3; NM_012177.5; NP_036309.1

UCSC genome browser

More...UCSCi		uc003qpg.4, human [Q9UKT4-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129535 mRNA Translation: AAF04469.1
AY079515 mRNA Translation: AAL86610.1
AK055221 mRNA Translation: BAG51487.1
AL080276 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47719.1
BC018905 mRNA Translation: AAH18905.1
CCDSiCCDS47501.1 [Q9UKT4-2]
CCDS5242.1 [Q9UKT4-1]
RefSeqiNP_001135994.1, NM_001142522.2 [Q9UKT4-2]
NP_036309.1, NM_012177.4 [Q9UKT4-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M6NNMR-A364-447[»]
4UI9electron microscopy3.60S1-447[»]
U1-27[»]
BMRBiQ9UKT4
SMRiQ9UKT4
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi117655, 54 interactors
DIPiDIP-38023N
ELMiQ9UKT4
IntActiQ9UKT4, 26 interactors
MINTiQ9UKT4
STRINGi9606.ENSP00000229758

PTM databases

GlyGeniQ9UKT4, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ9UKT4
PhosphoSitePlusiQ9UKT4

Genetic variation databases

BioMutaiFBXO5
DMDMi24636847

Proteomic databases

EPDiQ9UKT4
jPOSTiQ9UKT4
MassIVEiQ9UKT4
MaxQBiQ9UKT4
PaxDbiQ9UKT4
PeptideAtlasiQ9UKT4
PRIDEiQ9UKT4
ProteomicsDBi84847 [Q9UKT4-1]
84848 [Q9UKT4-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		33385, 243 antibodies from 29 providers

The DNASU plasmid repository

More...DNASUi		26271

Genome annotation databases

EnsembliENST00000229758; ENSP00000229758; ENSG00000112029
ENST00000367241; ENSP00000356210; ENSG00000112029 [Q9UKT4-2]
GeneIDi26271
KEGGihsa:26271
MANE-SelectiENST00000229758.8; ENSP00000229758.3; NM_012177.5; NP_036309.1
UCSCiuc003qpg.4, human [Q9UKT4-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		26271
DisGeNETi26271

GeneCards: human genes, protein and diseases

More...GeneCardsi		FBXO5
HGNCiHGNC:13584, FBXO5
HPAiENSG00000112029, Group enriched (bone marrow, lymphoid tissue)
MIMi606013, gene
neXtProtiNX_Q9UKT4
OpenTargetsiENSG00000112029
PharmGKBiPA28045
VEuPathDBiHostDB:ENSG00000112029

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiENOG502QPWN, Eukaryota
GeneTreeiENSGT00530000063692
HOGENOMiCLU_055946_0_0_1
InParanoidiQ9UKT4
OMAiIKFSPHA
OrthoDBi521317at2759
PhylomeDBiQ9UKT4
TreeFamiTF101170

Enzyme and pathway databases

UniPathwayiUPA00143
PathwayCommonsiQ9UKT4
ReactomeiR-HSA-174113, SCF-beta-TrCP mediated degradation of Emi1
R-HSA-176408, Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176417, Phosphorylation of Emi1
R-HSA-68881, Mitotic Metaphase/Anaphase Transition
R-HSA-69205, G1/S-Specific Transcription
SignaLinkiQ9UKT4
SIGNORiQ9UKT4

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		26271, 731 hits in 1095 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		FBXO5, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		FBXO5

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		26271
PharosiQ9UKT4, Tbio

Protein Ontology

More...PROi		PR:Q9UKT4
RNActiQ9UKT4, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000112029, Expressed in ventricular zone and 184 other tissues
GenevisibleiQ9UKT4, HS

Family and domain databases

DisProtiDP01450
InterProiView protein in InterPro
IPR001810, F-box_dom
IPR044064, ZF_ZBR
PfamiView protein in Pfam
PF00646, F-box, 1 hit
PROSITEiView protein in PROSITE
PS51872, ZF_ZBR, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFBX5_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9UKT4
Secondary accession number(s): B3KNX5
, Q5TF47, Q8WV29, Q9UGC8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: May 1, 2000
Last modified: February 23, 2022
This is version 173 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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