UniProtKB - Q9UKS6 (PACN3_HUMAN)
Protein
Protein kinase C and casein kinase substrate in neurons protein 3
Gene
PACSIN3
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity (By similarity).By similarity1 Publication
GO - Molecular functioni
- calcium channel inhibitor activity Source: UniProtKB
- cytoskeletal protein binding Source: UniProtKB
- identical protein binding Source: IntAct
- lipid binding Source: UniProtKB
- phospholipid binding Source: GO_Central
GO - Biological processi
- cytoskeleton organization Source: GO_Central
- endocytosis Source: UniProtKB-KW
- negative regulation of calcium ion transport Source: UniProtKB
- negative regulation of endocytosis Source: UniProtKB
- plasma membrane tubulation Source: UniProtKB
- positive regulation of membrane protein ectodomain proteolysis Source: BHF-UCL
- regulation of endocytosis Source: GO_Central
Keywordsi
Biological process | Endocytosis |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9UKS6 |
Reactomei | R-HSA-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Protein kinase C and casein kinase substrate in neurons protein 3Alternative name(s): SH3 domain-containing protein 6511 |
Gene namesi | Name:PACSIN3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8572, PACSIN3 |
MIMi | 606513, gene |
neXtProti | NX_Q9UKS6 |
VEuPathDBi | HostDB:ENSG00000165912.15 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Detected at the inner aspect of the plasma membrane in myotubes.By similarity
Cytoskeleton
- cytoskeleton Source: GO_Central
Cytosol
- cytosol Source: HPA
Endosome
- endosome Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 29763 |
OpenTargetsi | ENSG00000165912 |
PharmGKBi | PA32898 |
Miscellaneous databases
Pharosi | Q9UKS6, Tbio |
Genetic variation databases
BioMutai | PACSIN3 |
DMDMi | 22256967 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161800 | 1 – 424 | Protein kinase C and casein kinase substrate in neurons protein 3Add BLAST | 424 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | PhosphoserineCombined sources | 1 | |
Modified residuei | 303 | PhosphoserineBy similarity | 1 | |
Modified residuei | 319 | PhosphoserineCombined sources | 1 | |
Modified residuei | 324 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 327 | PhosphoserineCombined sources | 1 | |
Modified residuei | 354 | PhosphoserineCombined sources | 1 | |
Modified residuei | 383 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9UKS6 |
jPOSTi | Q9UKS6 |
MassIVEi | Q9UKS6 |
MaxQBi | Q9UKS6 |
PaxDbi | Q9UKS6 |
PeptideAtlasi | Q9UKS6 |
PRIDEi | Q9UKS6 |
ProteomicsDBi | 84843 |
PTM databases
iPTMneti | Q9UKS6 |
PhosphoSitePlusi | Q9UKS6 |
SwissPalmi | Q9UKS6 |
Expressioni
Tissue specificityi
Widely expressed, with highest levels in heart and skeletal muscle, intermediate levels in placenta, liver and pancreas, and very low levels in brain, lung and kidney.2 Publications
Gene expression databases
Bgeei | ENSG00000165912, Expressed in heart left ventricle and 187 other tissues |
ExpressionAtlasi | Q9UKS6, baseline and differential |
Genevisiblei | Q9UKS6, HS |
Organism-specific databases
HPAi | ENSG00000165912, Group enriched (heart muscle, skeletal muscle, tongue) |
Interactioni
Subunit structurei
Homodimer. May form heterooligomers with other PACSINs.
Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL.
Interacts with TRPV4 (By similarity).
By similarityBinary interactionsi
Hide detailsQ9UKS6
GO - Molecular functioni
- cytoskeletal protein binding Source: UniProtKB
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 118896, 102 interactors |
IntActi | Q9UKS6, 56 interactors |
MINTi | Q9UKS6 |
STRINGi | 9606.ENSP00000440945 |
Miscellaneous databases
RNActi | Q9UKS6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 280 | F-BARPROSITE-ProRule annotationAdd BLAST | 271 | |
Domaini | 363 – 424 | SH3PROSITE-ProRule annotationAdd BLAST | 62 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 23 – 272 | By similarityAdd BLAST | 250 |
Domaini
The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation.1 Publication
Sequence similaritiesi
Belongs to the PACSIN family.Curated
Keywords - Domaini
Coiled coil, SH3 domainPhylogenomic databases
eggNOGi | KOG2856, Eukaryota |
GeneTreei | ENSGT00950000182973 |
HOGENOMi | CLU_030752_2_0_1 |
InParanoidi | Q9UKS6 |
OMAi | ESCQAAE |
OrthoDBi | 727724at2759 |
PhylomeDBi | Q9UKS6 |
TreeFami | TF313677 |
Family and domain databases
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR031160, F_BAR IPR001060, FCH_dom IPR028523, PACSIN3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR23065:SF18, PTHR23065:SF18, 1 hit |
Pfami | View protein in Pfam PF00611, FCH, 1 hit PF14604, SH3_9, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00055, FCH, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51741, F_BAR, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q9UKS6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPEEDAGGE ALGGSFWEAG NYRRTVQRVE DGHRLCGDLV SCFQERARIE
60 70 80 90 100
KAYAQQLADW ARKWRGTVEK GPQYGTLEKA WHAFFTAAER LSALHLEVRE
110 120 130 140 150
KLQGQDSERV RAWQRGAFHR PVLGGFRESR AAEDGFRKAQ KPWLKRLKEV
160 170 180 190 200
EASKKSYHAA RKDEKTAQTR ESHAKADSAV SQEQLRKLQE RVERCAKEAE
210 220 230 240 250
KTKAQYEQTL AELHRYTPRY MEDMEQAFET CQAAERQRLL FFKDMLLTLH
260 270 280 290 300
QHLDLSSSEK FHELHRDLHQ GIEAASDEED LRWWRSTHGP GMAMNWPQFE
310 320 330 340 350
EWSLDTQRTI SRKEKGGRSP DEVTLTSIVP TRDGTAPPPQ SPGSPGTGQD
360 370 380 390 400
EEWSDEESPR KAATGVRVRA LYDYAGQEAD ELSFRAGEEL LKMSEEDEQG
410 420
WCQGQLQSGR IGLYPANYVE CVGA
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DGG1 | A0A0C4DGG1_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 405 | Annotation score: | ||
E9PIY1 | E9PIY1_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 320 | Annotation score: | ||
H0YDM6 | H0YDM6_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 76 | Annotation score: | ||
E9PNM9 | E9PNM9_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 148 | Annotation score: | ||
E9PJ75 | E9PJ75_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 142 | Annotation score: | ||
E9PJ33 | E9PJ33_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 69 | Annotation score: | ||
E9PIZ6 | E9PIZ6_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 48 | Annotation score: | ||
E9PQ12 | E9PQ12_HUMAN | Protein kinase C and casein kinase ... | PACSIN3 | 36 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053556 | 61 | A → V1 PublicationCorresponds to variant dbSNP:rs7106654Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF130979 mRNA Translation: AAF04472.1 AF149825 mRNA Translation: AAG31023.1 AF242530 mRNA Translation: AAK29207.1 AC090589 Genomic DNA No translation available. BC007914 mRNA Translation: AAH07914.1 BC011889 mRNA Translation: AAH11889.1 AK000577 mRNA Translation: BAA91267.1 |
CCDSi | CCDS31481.1 |
RefSeqi | NP_001171903.1, NM_001184974.1 NP_001171904.1, NM_001184975.1 NP_057307.2, NM_016223.4 |
Genome annotation databases
Ensembli | ENST00000298838; ENSP00000298838; ENSG00000165912 ENST00000539589; ENSP00000440945; ENSG00000165912 |
GeneIDi | 29763 |
KEGGi | hsa:29763 |
UCSCi | uc001ndw.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF130979 mRNA Translation: AAF04472.1 AF149825 mRNA Translation: AAG31023.1 AF242530 mRNA Translation: AAK29207.1 AC090589 Genomic DNA No translation available. BC007914 mRNA Translation: AAH07914.1 BC011889 mRNA Translation: AAH11889.1 AK000577 mRNA Translation: BAA91267.1 |
CCDSi | CCDS31481.1 |
RefSeqi | NP_001171903.1, NM_001184974.1 NP_001171904.1, NM_001184975.1 NP_057307.2, NM_016223.4 |
3D structure databases
SMRi | Q9UKS6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 118896, 102 interactors |
IntActi | Q9UKS6, 56 interactors |
MINTi | Q9UKS6 |
STRINGi | 9606.ENSP00000440945 |
PTM databases
iPTMneti | Q9UKS6 |
PhosphoSitePlusi | Q9UKS6 |
SwissPalmi | Q9UKS6 |
Genetic variation databases
BioMutai | PACSIN3 |
DMDMi | 22256967 |
Proteomic databases
EPDi | Q9UKS6 |
jPOSTi | Q9UKS6 |
MassIVEi | Q9UKS6 |
MaxQBi | Q9UKS6 |
PaxDbi | Q9UKS6 |
PeptideAtlasi | Q9UKS6 |
PRIDEi | Q9UKS6 |
ProteomicsDBi | 84843 |
Protocols and materials databases
Antibodypediai | 26590, 460 antibodies |
DNASUi | 29763 |
Genome annotation databases
Ensembli | ENST00000298838; ENSP00000298838; ENSG00000165912 ENST00000539589; ENSP00000440945; ENSG00000165912 |
GeneIDi | 29763 |
KEGGi | hsa:29763 |
UCSCi | uc001ndw.4, human |
Organism-specific databases
CTDi | 29763 |
DisGeNETi | 29763 |
GeneCardsi | PACSIN3 |
HGNCi | HGNC:8572, PACSIN3 |
HPAi | ENSG00000165912, Group enriched (heart muscle, skeletal muscle, tongue) |
MIMi | 606513, gene |
neXtProti | NX_Q9UKS6 |
OpenTargetsi | ENSG00000165912 |
PharmGKBi | PA32898 |
VEuPathDBi | HostDB:ENSG00000165912.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2856, Eukaryota |
GeneTreei | ENSGT00950000182973 |
HOGENOMi | CLU_030752_2_0_1 |
InParanoidi | Q9UKS6 |
OMAi | ESCQAAE |
OrthoDBi | 727724at2759 |
PhylomeDBi | Q9UKS6 |
TreeFami | TF313677 |
Enzyme and pathway databases
PathwayCommonsi | Q9UKS6 |
Reactomei | R-HSA-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 29763, 8 hits in 990 CRISPR screens |
ChiTaRSi | PACSIN3, human |
GeneWikii | PACSIN3 |
GenomeRNAii | 29763 |
Pharosi | Q9UKS6, Tbio |
PROi | PR:Q9UKS6 |
RNActi | Q9UKS6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165912, Expressed in heart left ventricle and 187 other tissues |
ExpressionAtlasi | Q9UKS6, baseline and differential |
Genevisiblei | Q9UKS6, HS |
Family and domain databases
Gene3Di | 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR031160, F_BAR IPR001060, FCH_dom IPR028523, PACSIN3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR23065:SF18, PTHR23065:SF18, 1 hit |
Pfami | View protein in Pfam PF00611, FCH, 1 hit PF14604, SH3_9, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00055, FCH, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51741, F_BAR, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PACN3_HUMAN | |
Accessioni | Q9UKS6Primary (citable) accession number: Q9UKS6 Secondary accession number(s): A6NH84, Q9H331, Q9NWV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 2002 |
Last sequence update: | August 13, 2002 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families