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UniProtKB - Q9UKG9 (OCTC_HUMAN)
Protein
Peroxisomal carnitine O-octanoyltransferase
Gene
CROT
Organism
Homo sapiens (Human)
Status
Functioni
Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.
1 PublicationCatalytic activityi
- EC:2.3.1.1371 Publication
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 327 | Proton acceptorBy similarity | 1 | |
Binding sitei | 406 | Coenzyme ABy similarity | 1 | |
Binding sitei | 439 | CarnitineBy similarity | 1 | |
Binding sitei | 441 | Carnitine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 452 | CarnitineBy similarity | 1 |
GO - Molecular functioni
- carnitine O-octanoyltransferase activity Source: UniProtKB
GO - Biological processi
- carnitine metabolic process Source: UniProtKB
- coenzyme A metabolic process Source: UniProtKB
- fatty acid beta-oxidation Source: UniProtKB
- fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
- fatty acid metabolic process Source: UniProtKB
- fatty acid transport Source: Ensembl
- generation of precursor metabolites and energy Source: UniProtKB
- medium-chain fatty acid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism, Transport |
Enzyme and pathway databases
BRENDAi | 2.3.1.137, 2681 |
PathwayCommonsi | Q9UKG9 |
Reactomei | R-HSA-389887, Beta-oxidation of pristanoyl-CoA R-HSA-9033241, Peroxisomal protein import |
SignaLinki | Q9UKG9 |
UniPathwayi | UPA00659 |
Protein family/group databases
TCDBi | 4.C.2.1.2, the carnitine o-acyl transferase (carat) family |
Chemistry databases
SwissLipidsi | SLP:000001055 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal carnitine O-octanoyltransferase (EC:2.3.1.1371 Publication)Short name: COT |
Gene namesi | Name:CROT Synonyms:COT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2366, CROT |
MIMi | 606090, gene |
neXtProti | NX_Q9UKG9 |
VEuPathDBi | HostDB:ENSG00000005469 |
Subcellular locationi
Peroxisome
- Peroxisome Curated
Cytosol
- cytosol Source: Reactome
Peroxisome
- peroxisomal matrix Source: Reactome
- peroxisome Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Organism-specific databases
DisGeNETi | 54677 |
OpenTargetsi | ENSG00000005469 |
PharmGKBi | PA26887 |
Miscellaneous databases
Pharosi | Q9UKG9, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2206 |
DrugBanki | DB02648, (3-Carboxy-2-(R)-Hydroxy-Propyl)-Trimethyl-Ammonium DB03832, 3-Carboxy-N,N,N-Trimethyl-2-(Octanoyloxy)Propan-1-Aminium DB00583, Levocarnitine |
Genetic variation databases
BioMutai | CROT |
DMDMi | 48429265 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210169 | 1 – 612 | Peroxisomal carnitine O-octanoyltransferaseAdd BLAST | 612 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 40 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 57 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 406 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 406 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9UKG9 |
jPOSTi | Q9UKG9 |
MassIVEi | Q9UKG9 |
MaxQBi | Q9UKG9 |
PaxDbi | Q9UKG9 |
PeptideAtlasi | Q9UKG9 |
PRIDEi | Q9UKG9 |
ProteomicsDBi | 17560 69947 84787 [Q9UKG9-1] |
PTM databases
iPTMneti | Q9UKG9 |
MetOSitei | Q9UKG9 |
PhosphoSitePlusi | Q9UKG9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000005469, Expressed in placenta and 239 other tissues |
ExpressionAtlasi | Q9UKG9, baseline and differential |
Genevisiblei | Q9UKG9, HS |
Organism-specific databases
HPAi | ENSG00000005469, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
Isoform 2 [Q9UKG9-2]
Protein-protein interaction databases
BioGRIDi | 120098, 14 interactors |
IntActi | Q9UKG9, 14 interactors |
STRINGi | 9606.ENSP00000413575 |
Chemistry databases
BindingDBi | Q9UKG9 |
Miscellaneous databases
RNActi | Q9UKG9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 410 – 417 | Coenzyme A bindingBy similarity | 8 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 610 – 612 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the carnitine/choline acetyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG3718, Eukaryota |
GeneTreei | ENSGT01040000240407 |
HOGENOMi | CLU_2482687_0_0_1 |
InParanoidi | Q9UKG9 |
OMAi | DVWAKDY |
OrthoDBi | 559299at2759 |
PhylomeDBi | Q9UKG9 |
TreeFami | TF313836 |
Family and domain databases
Gene3Di | 1.10.275.20, 1 hit 3.30.559.10, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR042572, Carn_acyl_trans_N IPR023213, CAT-like_dom_sf IPR039551, Cho/carn_acyl_trans IPR042231, Cho/carn_acyl_trans_2 |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9UKG9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENQLAKSTE ERTFQYQDSL PSLPVPSLEE SLKKYLESVK PFANQEEYKK
60 70 80 90 100
TEEIVQKFQS GIGEKLHQKL LERAKGKRNW LEEWWLNVAY LDVRIPSQLN
110 120 130 140 150
VNFAGPAAHF EHYWPPKEGT QLERGSITLW HNLNYWQLLR KEKVPVHKVG
160 170 180 190 200
NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF RTESEGRSPN HIVVLCRGRA
210 220 230 240 250
FVFDVIHEGC LVTPPELLRQ LTYIHKKCHS EPDGPGIAAL TSEERTRWAK
260 270 280 290 300
AREYLIGLDP ENLALLEKIQ SSLLVYSMED SSPHVTPEDY SEIIAAILIG
310 320 330 340 350
DPTVRWGDKS YNLISFSNGV FGCNCDHAPF DAMIMVNISY YVDEKIFQNE
360 370 380 390 400
GRWKGSEKVR DIPLPEELIF IVDEKVLNDI NQAKAQYLRE ASDLQIAAYA
410 420 430 440 450
FTSFGKKLTK NKMLHPDTFI QLALQLAYYR LHGHPGCCYE TAMTRHFYHG
460 470 480 490 500
RTETMRSCTV EAVRWCQSMQ DPSVNLRERQ QKMLQAFAKH NKMMKDCSAG
510 520 530 540 550
KGFDRHLLGL LLIAKEEGLP VPELFTDPLF SKSGGGGNFV LSTSLVGYLR
560 570 580 590 600
VQGVVVPMVH NGYGFFYHIR DDRFVVACSA WKSCPETDAE KLVQLTFCAF
610
HDMIQLMNST HL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9J3D7 | C9J3D7_HUMAN | Peroxisomal carnitine O-octanoyltra... | CROT | 574 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 144 | V → L in AAF03234 (PubMed:10486279).Curated | 1 | |
Sequence conflicti | 168 | V → G in AAD41654 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048612 | 94 | R → H. Corresponds to variant dbSNP:rs3827653Ensembl. | 1 | |
Natural variantiVAR_048613 | 474 | V → L. Corresponds to variant dbSNP:rs7785206Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046953 | 38 | S → SVTRTCYQIRGLDPDAKRGF LDLTREGIQ in isoform 3. Curated | 1 | |
Alternative sequenceiVSP_045213 | 81 – 87 | LEEWWLN → VFVVIIE in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_045214 | 88 – 612 | Missing in isoform 2. 1 PublicationAdd BLAST | 525 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168793 mRNA Translation: AAF03234.1 AF073770 mRNA Translation: AAD41654.1 AC005045 Genomic DNA No translation available. CH236949 Genomic DNA Translation: EAL24177.1 CH471091 Genomic DNA Translation: EAW76954.1 BC039004 mRNA Translation: AAH39004.1 BC051874 mRNA Translation: AAH51874.1 |
CCDSi | CCDS47634.1 [Q9UKG9-3] CCDS5604.1 [Q9UKG9-1] CCDS59062.1 [Q9UKG9-2] |
PIRi | JC7101 |
RefSeqi | NP_001137407.1, NM_001143935.1 [Q9UKG9-3] NP_001230674.1, NM_001243745.1 [Q9UKG9-2] NP_066974.2, NM_021151.3 [Q9UKG9-1] |
Genome annotation databases
Ensembli | ENST00000331536.8; ENSP00000331981.4; ENSG00000005469.12 ENST00000412227.6; ENSP00000404867.2; ENSG00000005469.12 [Q9UKG9-2] ENST00000419147.6; ENSP00000413575.2; ENSG00000005469.12 [Q9UKG9-3] |
GeneIDi | 54677 |
KEGGi | hsa:54677 |
MANE-Selecti | ENST00000331536.8; ENSP00000331981.4; NM_021151.4; NP_066974.2 |
UCSCi | uc003uis.4, human [Q9UKG9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168793 mRNA Translation: AAF03234.1 AF073770 mRNA Translation: AAD41654.1 AC005045 Genomic DNA No translation available. CH236949 Genomic DNA Translation: EAL24177.1 CH471091 Genomic DNA Translation: EAW76954.1 BC039004 mRNA Translation: AAH39004.1 BC051874 mRNA Translation: AAH51874.1 |
CCDSi | CCDS47634.1 [Q9UKG9-3] CCDS5604.1 [Q9UKG9-1] CCDS59062.1 [Q9UKG9-2] |
PIRi | JC7101 |
RefSeqi | NP_001137407.1, NM_001143935.1 [Q9UKG9-3] NP_001230674.1, NM_001243745.1 [Q9UKG9-2] NP_066974.2, NM_021151.3 [Q9UKG9-1] |
3D structure databases
AlphaFoldDBi | Q9UKG9 |
SMRi | Q9UKG9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120098, 14 interactors |
IntActi | Q9UKG9, 14 interactors |
STRINGi | 9606.ENSP00000413575 |
Chemistry databases
BindingDBi | Q9UKG9 |
ChEMBLi | CHEMBL2206 |
DrugBanki | DB02648, (3-Carboxy-2-(R)-Hydroxy-Propyl)-Trimethyl-Ammonium DB03832, 3-Carboxy-N,N,N-Trimethyl-2-(Octanoyloxy)Propan-1-Aminium DB00583, Levocarnitine |
SwissLipidsi | SLP:000001055 |
Protein family/group databases
TCDBi | 4.C.2.1.2, the carnitine o-acyl transferase (carat) family |
PTM databases
iPTMneti | Q9UKG9 |
MetOSitei | Q9UKG9 |
PhosphoSitePlusi | Q9UKG9 |
Genetic variation databases
BioMutai | CROT |
DMDMi | 48429265 |
Proteomic databases
EPDi | Q9UKG9 |
jPOSTi | Q9UKG9 |
MassIVEi | Q9UKG9 |
MaxQBi | Q9UKG9 |
PaxDbi | Q9UKG9 |
PeptideAtlasi | Q9UKG9 |
PRIDEi | Q9UKG9 |
ProteomicsDBi | 17560 69947 84787 [Q9UKG9-1] |
Protocols and materials databases
Antibodypediai | 15286, 344 antibodies from 34 providers |
DNASUi | 54677 |
Genome annotation databases
Ensembli | ENST00000331536.8; ENSP00000331981.4; ENSG00000005469.12 ENST00000412227.6; ENSP00000404867.2; ENSG00000005469.12 [Q9UKG9-2] ENST00000419147.6; ENSP00000413575.2; ENSG00000005469.12 [Q9UKG9-3] |
GeneIDi | 54677 |
KEGGi | hsa:54677 |
MANE-Selecti | ENST00000331536.8; ENSP00000331981.4; NM_021151.4; NP_066974.2 |
UCSCi | uc003uis.4, human [Q9UKG9-1] |
Organism-specific databases
CTDi | 54677 |
DisGeNETi | 54677 |
GeneCardsi | CROT |
HGNCi | HGNC:2366, CROT |
HPAi | ENSG00000005469, Low tissue specificity |
MIMi | 606090, gene |
neXtProti | NX_Q9UKG9 |
OpenTargetsi | ENSG00000005469 |
PharmGKBi | PA26887 |
VEuPathDBi | HostDB:ENSG00000005469 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3718, Eukaryota |
GeneTreei | ENSGT01040000240407 |
HOGENOMi | CLU_2482687_0_0_1 |
InParanoidi | Q9UKG9 |
OMAi | DVWAKDY |
OrthoDBi | 559299at2759 |
PhylomeDBi | Q9UKG9 |
TreeFami | TF313836 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
BRENDAi | 2.3.1.137, 2681 |
PathwayCommonsi | Q9UKG9 |
Reactomei | R-HSA-389887, Beta-oxidation of pristanoyl-CoA R-HSA-9033241, Peroxisomal protein import |
SignaLinki | Q9UKG9 |
Miscellaneous databases
BioGRID-ORCSi | 54677, 13 hits in 1084 CRISPR screens |
ChiTaRSi | CROT, human |
GeneWikii | CROT_(gene) |
GenomeRNAii | 54677 |
Pharosi | Q9UKG9, Tbio |
PROi | PR:Q9UKG9 |
RNActi | Q9UKG9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000005469, Expressed in placenta and 239 other tissues |
ExpressionAtlasi | Q9UKG9, baseline and differential |
Genevisiblei | Q9UKG9, HS |
Family and domain databases
Gene3Di | 1.10.275.20, 1 hit 3.30.559.10, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR042572, Carn_acyl_trans_N IPR023213, CAT-like_dom_sf IPR039551, Cho/carn_acyl_trans IPR042231, Cho/carn_acyl_trans_2 |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OCTC_HUMAN | |
Accessioni | Q9UKG9Primary (citable) accession number: Q9UKG9 Secondary accession number(s): A4D1D6 Q9Y6I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | June 7, 2004 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families