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Protein

F-box only protein 3

Gene

FBXO3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.1 Publication

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: ProtInc

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 3
Gene namesi
Name:FBXO3
Synonyms:FBX3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110429.13
HGNCiHGNC:13582 FBXO3
MIMi609089 gene
neXtProtiNX_Q9UK99

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26273
OpenTargetsiENSG00000110429
PharmGKBiPA28040

Polymorphism and mutation databases

BioMutaiFBXO3
DMDMi145559474

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198771 – 471F-box only protein 3Add BLAST471

Proteomic databases

EPDiQ9UK99
PaxDbiQ9UK99
PeptideAtlasiQ9UK99
PRIDEiQ9UK99
ProteomicsDBi84745
84746 [Q9UK99-2]
84747 [Q9UK99-3]

PTM databases

iPTMnetiQ9UK99
PhosphoSitePlusiQ9UK99

Expressioni

Gene expression databases

BgeeiENSG00000110429 Expressed in 227 organ(s), highest expression level in skeletal muscle tissue of rectus abdominis
CleanExiHS_FBXO3
ExpressionAtlasiQ9UK99 baseline and differential
GenevisibleiQ9UK99 HS

Organism-specific databases

HPAiHPA002467

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi117657, 37 interactors
DIPiDIP-53688N
IntActiQ9UK99, 8 interactors
MINTiQ9UK99
STRINGi9606.ENSP00000265651

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UK99
SMRiQ9UK99
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 56F-boxPROSITE-ProRule annotationAdd BLAST47
Domaini278 – 408ApaGPROSITE-ProRule annotationAdd BLAST131

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi418 – 452Asp/Glu-rich (highly acidic)Add BLAST35
Compositional biasi453 – 456Poly-Arg4
Compositional biasi463 – 466Poly-Arg4

Phylogenomic databases

eggNOGiKOG4408 Eukaryota
COG2967 LUCA
GeneTreeiENSGT00390000009225
HOGENOMiHOG000232174
HOVERGENiHBG005647
InParanoidiQ9UK99
KOiK10290
OMAiDLGRYIQ
OrthoDBiEOG091G08GB
PhylomeDBiQ9UK99
TreeFamiTF329795

Family and domain databases

Gene3Di2.60.40.1470, 1 hit
3.40.1580.10, 1 hit
InterProiView protein in InterPro
IPR007474 ApaG_domain
IPR036767 ApaG_sf
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR018958 Knr4/Smi1-like_dom
IPR037883 Knr4/Smi1-like_sf
PfamiView protein in Pfam
PF04379 DUF525, 1 hit
PF12937 F-box-like, 1 hit
PF09346 SMI1_KNR4, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00860 SMI1_KNR4, 1 hit
SUPFAMiSSF110069 SSF110069, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51087 APAG, 1 hit
PS50181 FBOX, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UK99-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAMETETAP LTLESLPTDP LLLILSFLDY RDLINCCYVS RRLSQLSSHD
60 70 80 90 100
PLWRRHCKKY WLISEEEKTQ KNQCWKSLFI DTYSDVGRYI DHYAAIKKAW
110 120 130 140 150
DDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN
160 170 180 190 200
GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI
210 220 230 240 250
HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT
260 270 280 290 300
SYVKNVVSGG FPIIRDQIFR YVHDPECVAT TGDITVSVST SFLPELSSVH
310 320 330 340 350
PPHYFFTYRI RIEMSKDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG
360 370 380 390 400
EFPIISPGRV YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK IFNVAIPRFH
410 420 430 440 450
MACPTFRVSI ARLEMGPDEY EEMEEEEEEE EEEDEDDDSA DMDESDEDDE
460 470
EERRRRVFDV PIRRRRCSRL F
Length:471
Mass (Da):54,561
Last modified:April 17, 2007 - v3
Checksum:i5FDA81669264E0AD
GO
Isoform 2 (identifier: Q9UK99-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     414-415: EM → VS
     416-471: Missing.

Note: No experimental confirmation available.
Show »
Length:415
Mass (Da):47,530
Checksum:i9CA7AC918B28FCB0
GO
Isoform 3 (identifier: Q9UK99-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-40: Missing.
     414-415: EM → VS
     416-471: Missing.

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):46,975
Checksum:i221BD13D1AB4236E
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V1E0G3V1E0_HUMAN
F-box only protein 3
FBXO3 hCG_27567
358Annotation score:
E9PJM3E9PJM3_HUMAN
F-box only protein 3
FBXO3
430Annotation score:
Q49AF1Q49AF1_HUMAN
F-box only protein 3
FBXO3
158Annotation score:
B4E3U2B4E3U2_HUMAN
cDNA FLJ59154, highly similar to F-...
FBXO3
172Annotation score:
E9PL46E9PL46_HUMAN
F-box only protein 3
FBXO3
129Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28L → M in CAB66560 (PubMed:11230166).Curated1
Sequence conflicti96 – 98IKK → VKR in CAB66560 (PubMed:11230166).Curated3
Sequence conflicti164A → T in BAA91991 (PubMed:14702039).Curated1
Sequence conflicti227D → G in CAB66560 (PubMed:11230166).Curated1
Sequence conflicti239F → L in CAB66560 (PubMed:11230166).Curated1
Sequence conflicti378G → E in CAB66560 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049037221V → I. Corresponds to variant dbSNP:rs1402954Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03961436 – 40Missing in isoform 3. 1 Publication5
Alternative sequenceiVSP_024395414 – 415EM → VS in isoform 2 and isoform 3. 2 Publications2
Alternative sequenceiVSP_024396416 – 471Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001943 mRNA Translation: BAA91991.1
AK128454 mRNA Translation: BAG54678.1
AL136625 mRNA Translation: CAB66560.1
AC113192 Genomic DNA No translation available.
AL049629 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68186.1
CH471064 Genomic DNA Translation: EAW68188.1
CH471064 Genomic DNA Translation: EAW68189.1
BC046110 mRNA Translation: AAH46110.1
AF176702 mRNA Translation: AAF03702.1
CCDSiCCDS44566.1 [Q9UK99-2]
CCDS7887.1 [Q9UK99-1]
RefSeqiNP_036307.2, NM_012175.3 [Q9UK99-1]
NP_208385.1, NM_033406.2 [Q9UK99-2]
UniGeneiHs.406787

Genome annotation databases

EnsembliENST00000265651; ENSP00000265651; ENSG00000110429 [Q9UK99-1]
ENST00000448981; ENSP00000408836; ENSG00000110429 [Q9UK99-2]
ENST00000530401; ENSP00000433781; ENSG00000110429 [Q9UK99-3]
GeneIDi26273
KEGGihsa:26273
UCSCiuc001muz.4 human [Q9UK99-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001943 mRNA Translation: BAA91991.1
AK128454 mRNA Translation: BAG54678.1
AL136625 mRNA Translation: CAB66560.1
AC113192 Genomic DNA No translation available.
AL049629 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68186.1
CH471064 Genomic DNA Translation: EAW68188.1
CH471064 Genomic DNA Translation: EAW68189.1
BC046110 mRNA Translation: AAH46110.1
AF176702 mRNA Translation: AAF03702.1
CCDSiCCDS44566.1 [Q9UK99-2]
CCDS7887.1 [Q9UK99-1]
RefSeqiNP_036307.2, NM_012175.3 [Q9UK99-1]
NP_208385.1, NM_033406.2 [Q9UK99-2]
UniGeneiHs.406787

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HDWX-ray2.00A278-407[»]
ProteinModelPortaliQ9UK99
SMRiQ9UK99
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117657, 37 interactors
DIPiDIP-53688N
IntActiQ9UK99, 8 interactors
MINTiQ9UK99
STRINGi9606.ENSP00000265651

PTM databases

iPTMnetiQ9UK99
PhosphoSitePlusiQ9UK99

Polymorphism and mutation databases

BioMutaiFBXO3
DMDMi145559474

Proteomic databases

EPDiQ9UK99
PaxDbiQ9UK99
PeptideAtlasiQ9UK99
PRIDEiQ9UK99
ProteomicsDBi84745
84746 [Q9UK99-2]
84747 [Q9UK99-3]

Protocols and materials databases

DNASUi26273
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265651; ENSP00000265651; ENSG00000110429 [Q9UK99-1]
ENST00000448981; ENSP00000408836; ENSG00000110429 [Q9UK99-2]
ENST00000530401; ENSP00000433781; ENSG00000110429 [Q9UK99-3]
GeneIDi26273
KEGGihsa:26273
UCSCiuc001muz.4 human [Q9UK99-1]

Organism-specific databases

CTDi26273
DisGeNETi26273
EuPathDBiHostDB:ENSG00000110429.13
GeneCardsiFBXO3
HGNCiHGNC:13582 FBXO3
HPAiHPA002467
MIMi609089 gene
neXtProtiNX_Q9UK99
OpenTargetsiENSG00000110429
PharmGKBiPA28040
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4408 Eukaryota
COG2967 LUCA
GeneTreeiENSGT00390000009225
HOGENOMiHOG000232174
HOVERGENiHBG005647
InParanoidiQ9UK99
KOiK10290
OMAiDLGRYIQ
OrthoDBiEOG091G08GB
PhylomeDBiQ9UK99
TreeFamiTF329795

Miscellaneous databases

GenomeRNAii26273
PROiPR:Q9UK99
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110429 Expressed in 227 organ(s), highest expression level in skeletal muscle tissue of rectus abdominis
CleanExiHS_FBXO3
ExpressionAtlasiQ9UK99 baseline and differential
GenevisibleiQ9UK99 HS

Family and domain databases

Gene3Di2.60.40.1470, 1 hit
3.40.1580.10, 1 hit
InterProiView protein in InterPro
IPR007474 ApaG_domain
IPR036767 ApaG_sf
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR018958 Knr4/Smi1-like_dom
IPR037883 Knr4/Smi1-like_sf
PfamiView protein in Pfam
PF04379 DUF525, 1 hit
PF12937 F-box-like, 1 hit
PF09346 SMI1_KNR4, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SM00860 SMI1_KNR4, 1 hit
SUPFAMiSSF110069 SSF110069, 1 hit
SSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS51087 APAG, 1 hit
PS50181 FBOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBX3_HUMAN
AccessioniPrimary (citable) accession number: Q9UK99
Secondary accession number(s): B3KY16
, D3DR05, Q86X90, Q9H0V2, Q9NUX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 17, 2007
Last modified: November 7, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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