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Protein

Lariat debranching enzyme

Gene

DBR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis.2 Publications

Miscellaneous

Cells lacking DBR1 show an inhibition of HIV-1 replication.

Cofactori

a divalent metal cation1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • RNA lariat debranching enzyme activity Source: UniProtKB

GO - Biological processi

  • mRNA splicing, via spliceosome Source: GO_Central
  • RNA splicing, via transesterification reactions Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processmRNA processing
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lariat debranching enzyme (EC:3.1.-.-)
Gene namesi
Name:DBR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000138231.12
HGNCiHGNC:15594 DBR1
MIMi607024 gene
neXtProtiNX_Q9UK59

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi51163
OpenTargetsiENSG00000138231
PharmGKBiPA27166

Polymorphism and mutation databases

BioMutaiDBR1
DMDMi115311701

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002503581 – 544Lariat debranching enzymeAdd BLAST544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128N6-acetyllysineCombined sources1
Modified residuei464PhosphoserineCombined sources1
Modified residuei474PhosphoserineCombined sources1
Modified residuei478PhosphoserineCombined sources1
Modified residuei479PhosphoserineCombined sources1
Modified residuei485PhosphoserineBy similarity1
Modified residuei499PhosphoserineBy similarity1
Modified residuei514PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UK59
PaxDbiQ9UK59
PeptideAtlasiQ9UK59
PRIDEiQ9UK59
ProteomicsDBi84727
84728 [Q9UK59-2]

PTM databases

iPTMnetiQ9UK59
PhosphoSitePlusiQ9UK59

Expressioni

Gene expression databases

BgeeiENSG00000138231 Expressed in 202 organ(s), highest expression level in forebrain
CleanExiHS_DBR1
ExpressionAtlasiQ9UK59 baseline and differential
GenevisibleiQ9UK59 HS

Organism-specific databases

HPAiHPA035365

Interactioni

Protein-protein interaction databases

BioGridi119344, 14 interactors
IntActiQ9UK59, 6 interactors
STRINGi9606.ENSP00000260803

Structurei

3D structure databases

ProteinModelPortaliQ9UK59
SMRiQ9UK59
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lariat debranching enzyme family.Curated

Phylogenomic databases

eggNOGiKOG2863 Eukaryota
ENOG410XP0F LUCA
GeneTreeiENSGT00510000047481
HOGENOMiHOG000216468
HOVERGENiHBG079917
InParanoidiQ9UK59
KOiK18328
OMAiRINPQTT
OrthoDBiEOG091G0AE9
PhylomeDBiQ9UK59
TreeFamiTF313221

Family and domain databases

InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR007708 DBR1_C
PfamiView protein in Pfam
PF05011 DBR1, 1 hit
PF00149 Metallophos, 1 hit
SMARTiView protein in SMART
SM01124 DBR1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UK59-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRVAVAGCCH GELDKIYETL ALAERRGPGP VDLLLCCGDF QAVRNEADLR
60 70 80 90 100
CMAVPPKYRH MQTFYRYYSG EKKAPVLTLF IGGNHEASNH LQELPYGGWV
110 120 130 140 150
APNIYYLGLA GVVKYRGVRI GGISGIFKSH DYRKGHFECP PYNSSTIRSI
160 170 180 190 200
YHVRNIEVYK LKQLKQPIDI FLSHDWPRSI YHYGNKKQLL KTKSFFRQEV
210 220 230 240 250
ENNTLGSPAA SELLEHLKPT YWFSAHLHVK FAALMQHQAK DKGQTARATK
260 270 280 290 300
FLALDKCLPH RDFLQILEIE HDPSAPDYLE YDIEWLTILR ATDDLINVTG
310 320 330 340 350
RLWNMPENNG LHARWDYSAT EEGMKEVLEK LNHDLKVPCN FSVTAACYDP
360 370 380 390 400
SKPQTQMQLI HRINPQTTEF CAQLGIIDIN VRLQKSKEEH HVCGEYEEQD
410 420 430 440 450
DVESNDSGED QSEYNTDTSA LSSINPDEIM LDEEEDEDSI VSAHSGMNTP
460 470 480 490 500
SVEPSDQASE FSASFSDVRI LPGSMIVSSD DTVDSTIDRE GKPGGTVESG
510 520 530 540
NGEDLTKVPL KRLSDEHEPE QRKKIKRRNQ AIYAAVDDDD DDAA
Length:544
Mass (Da):61,555
Last modified:September 19, 2006 - v2
Checksum:iDDA5F4FADA194BAA
GO
Isoform 2 (identifier: Q9UK59-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.
     233-237: ALMQH → MIHIV

Note: No experimental confirmation available.
Show »
Length:312
Mass (Da):35,022
Checksum:i4E874B871647E9F5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WAY1F8WAY1_HUMAN
Lariat debranching enzyme
DBR1
118Annotation score:

Sequence cautioni

The sequence AAD53327 differs from that shown. Reason: Frameshift at positions 450 and 463.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti308N → S in BAA90954 (PubMed:14702039).Curated1
Sequence conflicti496T → L in AAD53327 (PubMed:10982890).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0206311 – 232Missing in isoform 2. 1 PublicationAdd BLAST232
Alternative sequenceiVSP_020632233 – 237ALMQH → MIHIV in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180919 mRNA Translation: AAD53327.2 Frameshift.
AK000116 mRNA Translation: BAA90954.1
BC009472 mRNA Translation: AAH09472.1
CCDSiCCDS33863.1 [Q9UK59-1]
RefSeqiNP_057300.2, NM_016216.3 [Q9UK59-1]
UniGeneiHs.477700

Genome annotation databases

EnsembliENST00000260803; ENSP00000260803; ENSG00000138231 [Q9UK59-1]
GeneIDi51163
KEGGihsa:51163
UCSCiuc003erv.4 human [Q9UK59-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180919 mRNA Translation: AAD53327.2 Frameshift.
AK000116 mRNA Translation: BAA90954.1
BC009472 mRNA Translation: AAH09472.1
CCDSiCCDS33863.1 [Q9UK59-1]
RefSeqiNP_057300.2, NM_016216.3 [Q9UK59-1]
UniGeneiHs.477700

3D structure databases

ProteinModelPortaliQ9UK59
SMRiQ9UK59
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119344, 14 interactors
IntActiQ9UK59, 6 interactors
STRINGi9606.ENSP00000260803

PTM databases

iPTMnetiQ9UK59
PhosphoSitePlusiQ9UK59

Polymorphism and mutation databases

BioMutaiDBR1
DMDMi115311701

Proteomic databases

EPDiQ9UK59
PaxDbiQ9UK59
PeptideAtlasiQ9UK59
PRIDEiQ9UK59
ProteomicsDBi84727
84728 [Q9UK59-2]

Protocols and materials databases

DNASUi51163
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260803; ENSP00000260803; ENSG00000138231 [Q9UK59-1]
GeneIDi51163
KEGGihsa:51163
UCSCiuc003erv.4 human [Q9UK59-1]

Organism-specific databases

CTDi51163
DisGeNETi51163
EuPathDBiHostDB:ENSG00000138231.12
GeneCardsiDBR1
HGNCiHGNC:15594 DBR1
HPAiHPA035365
MIMi607024 gene
neXtProtiNX_Q9UK59
OpenTargetsiENSG00000138231
PharmGKBiPA27166
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2863 Eukaryota
ENOG410XP0F LUCA
GeneTreeiENSGT00510000047481
HOGENOMiHOG000216468
HOVERGENiHBG079917
InParanoidiQ9UK59
KOiK18328
OMAiRINPQTT
OrthoDBiEOG091G0AE9
PhylomeDBiQ9UK59
TreeFamiTF313221

Miscellaneous databases

GeneWikiiDBR1
GenomeRNAii51163
PROiPR:Q9UK59
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138231 Expressed in 202 organ(s), highest expression level in forebrain
CleanExiHS_DBR1
ExpressionAtlasiQ9UK59 baseline and differential
GenevisibleiQ9UK59 HS

Family and domain databases

InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR007708 DBR1_C
PfamiView protein in Pfam
PF05011 DBR1, 1 hit
PF00149 Metallophos, 1 hit
SMARTiView protein in SMART
SM01124 DBR1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDBR1_HUMAN
AccessioniPrimary (citable) accession number: Q9UK59
Secondary accession number(s): Q96GH0, Q9NXQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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