UniProtKB - Q9UK41 (VPS28_HUMAN)
Vacuolar protein sorting-associated protein 28 homolog
VPS28
Functioni
GO - Molecular functioni
- protein-containing complex binding Source: GO_Central
- ubiquitin binding Source: UniProtKB
GO - Biological processi
- endosomal transport Source: Reactome
- intracellular transport of virus Source: Reactome
- macroautophagy Source: ParkinsonsUK-UCL
- multivesicular body assembly Source: ParkinsonsUK-UCL
- negative regulation of protein ubiquitination Source: UniProtKB
- positive regulation of protein catabolic process Source: UniProtKB
- positive regulation of ubiquitin-dependent endocytosis Source: UniProtKB
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
- viral budding via host ESCRT complex Source: ParkinsonsUK-UCL
- viral life cycle Source: Reactome
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9UK41 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-174490, Membrane binding and targetting of GAG proteins R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 28 homologShort name: H-Vps28 Alternative name(s): ESCRT-I complex subunit VPS28 |
Gene namesi | Name:VPS28 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18178, VPS28 |
MIMi | 611952, gene |
neXtProti | NX_Q9UK41 |
VEuPathDBi | HostDB:ENSG00000160948.13 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Endosome
- Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication
Cytosol
- cytosol Source: MGI
Endosome
- early endosome Source: UniProtKB
- endosome Source: UniProtKB
- endosome membrane Source: UniProtKB
- ESCRT I complex Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 51160 |
OpenTargetsi | ENSG00000160948 |
PharmGKBi | PA38512 |
Miscellaneous databases
Pharosi | Q9UK41, Tbio |
Genetic variation databases
BioMutai | VPS28 |
DMDMi | 13124619 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120951 | 1 – 221 | Vacuolar protein sorting-associated protein 28 homologAdd BLAST | 221 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9UK41 |
jPOSTi | Q9UK41 |
MassIVEi | Q9UK41 |
PaxDbi | Q9UK41 |
PeptideAtlasi | Q9UK41 |
PRIDEi | Q9UK41 |
ProteomicsDBi | 84715 [Q9UK41-1] 84716 [Q9UK41-2] |
TopDownProteomicsi | Q9UK41-1 [Q9UK41-1] |
PTM databases
iPTMneti | Q9UK41 |
MetOSitei | Q9UK41 |
PhosphoSitePlusi | Q9UK41 |
Expressioni
Gene expression databases
Bgeei | ENSG00000160948, Expressed in right testis and 241 other tissues |
ExpressionAtlasi | Q9UK41, baseline and differential |
Genevisiblei | Q9UK41, HS |
Organism-specific databases
HPAi | ENSG00000160948, Low tissue specificity |
Interactioni
Subunit structurei
Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry (PubMed:14505570, PubMed:18005716).
Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B (PubMed:14505570, PubMed:15218037, PubMed:18005716).
Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (PubMed:14505570, PubMed:21757351, PubMed:30929741).
Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex.
Interacts with SNF8 and VPS25 (PubMed:18539118).
Interacts with CEP55 (PubMed:17853893).
7 PublicationsBinary interactionsi
Hide detailsQ9UK41
Isoform 2 [Q9UK41-2]
GO - Molecular functioni
- ubiquitin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119341, 84 interactors |
CORUMi | Q9UK41 |
IntActi | Q9UK41, 68 interactors |
MINTi | Q9UK41 |
STRINGi | 9606.ENSP00000366565 |
Miscellaneous databases
RNActi | Q9UK41, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9UK41 |
SMRi | Q9UK41 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 120 | VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 124 – 220 | VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST | 97 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3284, Eukaryota |
GeneTreei | ENSGT00390000007486 |
HOGENOMi | CLU_076417_2_0_1 |
InParanoidi | Q9UK41 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9UK41 |
TreeFami | TF313364 |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ
60 70 80 90 100
ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR
110 120 130 140 150
LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM
160 170 180 190 200
DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ
210 220
VRQMLFDLES AYNAFNRFLH A
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PQR7 | E9PQR7_HUMAN | Vacuolar protein sorting-associated... | VPS28 | 161 | Annotation score: | ||
E9PM90 | E9PM90_HUMAN | Vacuolar protein sorting-associated... | VPS28 | 185 | Annotation score: | ||
E9PR04 | E9PR04_HUMAN | Vacuolar protein sorting-associated... | VPS28 | 53 | Annotation score: | ||
E9PLM9 | E9PLM9_HUMAN | Vacuolar protein sorting-associated... | VPS28 | 35 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042028 | 184 – 221 | LQTLS…RFLHA → WVSLPARQSPAVPETLPARR SPAVPLRPSAPTCPVLHSQA ADPERHVGVR in isoform 2. 1 PublicationAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF182844 mRNA Translation: AAF00499.1 AF205589 Genomic DNA No translation available. BC006485 mRNA Translation: AAH06485.1 BC019321 mRNA Translation: AAH19321.1 BC050713 mRNA Translation: AAH50713.1 |
CCDSi | CCDS34967.1 [Q9UK41-2] CCDS6425.1 [Q9UK41-1] |
RefSeqi | NP_057292.1, NM_016208.3 [Q9UK41-1] NP_898880.1, NM_183057.2 [Q9UK41-2] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF182844 mRNA Translation: AAF00499.1 AF205589 Genomic DNA No translation available. BC006485 mRNA Translation: AAH06485.1 BC019321 mRNA Translation: AAH19321.1 BC050713 mRNA Translation: AAH50713.1 |
CCDSi | CCDS34967.1 [Q9UK41-2] CCDS6425.1 [Q9UK41-1] |
RefSeqi | NP_057292.1, NM_016208.3 [Q9UK41-1] NP_898880.1, NM_183057.2 [Q9UK41-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6VME | X-ray | 2.19 | E/U/V/W/X/Y | 1-122 | [»] | |
BMRBi | Q9UK41 | |||||
SMRi | Q9UK41 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119341, 84 interactors |
CORUMi | Q9UK41 |
IntActi | Q9UK41, 68 interactors |
MINTi | Q9UK41 |
STRINGi | 9606.ENSP00000366565 |
PTM databases
iPTMneti | Q9UK41 |
MetOSitei | Q9UK41 |
PhosphoSitePlusi | Q9UK41 |
Genetic variation databases
BioMutai | VPS28 |
DMDMi | 13124619 |
Proteomic databases
EPDi | Q9UK41 |
jPOSTi | Q9UK41 |
MassIVEi | Q9UK41 |
PaxDbi | Q9UK41 |
PeptideAtlasi | Q9UK41 |
PRIDEi | Q9UK41 |
ProteomicsDBi | 84715 [Q9UK41-1] 84716 [Q9UK41-2] |
TopDownProteomicsi | Q9UK41-1 [Q9UK41-1] |
Protocols and materials databases
Antibodypediai | 14872, 193 antibodies |
DNASUi | 51160 |
Genome annotation databases
Organism-specific databases
CTDi | 51160 |
DisGeNETi | 51160 |
GeneCardsi | VPS28 |
HGNCi | HGNC:18178, VPS28 |
HPAi | ENSG00000160948, Low tissue specificity |
MIMi | 611952, gene |
neXtProti | NX_Q9UK41 |
OpenTargetsi | ENSG00000160948 |
PharmGKBi | PA38512 |
VEuPathDBi | HostDB:ENSG00000160948.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3284, Eukaryota |
GeneTreei | ENSGT00390000007486 |
HOGENOMi | CLU_076417_2_0_1 |
InParanoidi | Q9UK41 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9UK41 |
TreeFami | TF313364 |
Enzyme and pathway databases
PathwayCommonsi | Q9UK41 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-174490, Membrane binding and targetting of GAG proteins R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy |
Miscellaneous databases
BioGRID-ORCSi | 51160, 785 hits in 997 CRISPR screens |
ChiTaRSi | VPS28, human |
GeneWikii | VPS28 |
GenomeRNAii | 51160 |
Pharosi | Q9UK41, Tbio |
PROi | PR:Q9UK41 |
RNActi | Q9UK41, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000160948, Expressed in right testis and 241 other tissues |
ExpressionAtlasi | Q9UK41, baseline and differential |
Genevisiblei | Q9UK41, HS |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS28_HUMAN | |
Accessioni | Q9UK41Primary (citable) accession number: Q9UK41 Secondary accession number(s): Q86VK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families