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Protein

Vacuolar protein sorting-associated protein 28 homolog

Gene

VPS28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: UniProtKB

GO - Biological processi

  • endosomal transport Source: Reactome
  • macroautophagy Source: ParkinsonsUK-UCL
  • multivesicular body assembly Source: ParkinsonsUK-UCL
  • negative regulation of protein ubiquitination Source: UniProtKB
  • positive regulation of protein catabolic process Source: UniProtKB
  • positive regulation of ubiquitin-dependent endocytosis Source: UniProtKB
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
  • viral budding via host ESCRT complex Source: ParkinsonsUK-UCL
  • viral life cycle Source: Reactome

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-174490 Membrane binding and targetting of GAG proteins
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28 homolog
Short name:
H-Vps28
Alternative name(s):
ESCRT-I complex subunit VPS28
Gene namesi
Name:VPS28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000160948.13
HGNCiHGNC:18178 VPS28
MIMi611952 gene
neXtProtiNX_Q9UK41

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000160948
PharmGKBiPA38512

Polymorphism and mutation databases

DMDMi13124619

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001209511 – 221Vacuolar protein sorting-associated protein 28 homologAdd BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9UK41
PaxDbiQ9UK41
PeptideAtlasiQ9UK41
PRIDEiQ9UK41
ProteomicsDBi84715
84716 [Q9UK41-2]
TopDownProteomicsiQ9UK41-1 [Q9UK41-1]

PTM databases

iPTMnetiQ9UK41
PhosphoSitePlusiQ9UK41

Expressioni

Gene expression databases

BgeeiENSG00000160948
CleanExiHS_VPS28
ExpressionAtlasiQ9UK41 baseline and differential
GenevisibleiQ9UK41 HS

Organism-specific databases

HPAiHPA024688
HPA024745

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119341, 63 interactors
CORUMiQ9UK41
IntActiQ9UK41, 43 interactors
MINTiQ9UK41
STRINGi9606.ENSP00000366565

Structurei

3D structure databases

ProteinModelPortaliQ9UK41
SMRiQ9UK41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 120VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST108
Domaini124 – 220VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3284 Eukaryota
ENOG4111IQ4 LUCA
GeneTreeiENSGT00390000007486
HOGENOMiHOG000203818
HOVERGENiHBG054248
InParanoidiQ9UK41
KOiK12184
OMAiKYRLDCP
OrthoDBiEOG091G0LT0
PhylomeDBiQ9UK41
TreeFamiTF313364

Family and domain databases

Gene3Di1.20.120.1130, 1 hit
1.20.1440.200, 1 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR007143 Vps28
IPR017899 VPS28_C
IPR037206 VPS28_C_sf
IPR017898 VPS28_N
IPR038358 VPS28_N_sf
PANTHERiPTHR12937 PTHR12937, 1 hit
PfamiView protein in Pfam
PF03997 VPS28, 1 hit
PIRSFiPIRSF017535 VPS28, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF140427 SSF140427, 1 hit
PROSITEiView protein in PROSITE
PS51310 VPS28_C, 1 hit
PS51313 VPS28_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UK41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ
60 70 80 90 100
ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR
110 120 130 140 150
LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM
160 170 180 190 200
DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ
210 220
VRQMLFDLES AYNAFNRFLH A
Length:221
Mass (Da):25,425
Last modified:May 1, 2000 - v1
Checksum:iB2E1697B82D02AB8
GO
Isoform 2 (identifier: Q9UK41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-221: LQTLSGMSAS...YNAFNRFLHA → WVSLPARQSP...ADPERHVGVR

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,463
Checksum:i3EE28CC0503FCBF6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042028184 – 221LQTLS…RFLHA → WVSLPARQSPAVPETLPARR SPAVPLRPSAPTCPVLHSQA ADPERHVGVR in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182844 mRNA Translation: AAF00499.1
AF205589 Genomic DNA No translation available.
BC006485 mRNA Translation: AAH06485.1
BC019321 mRNA Translation: AAH19321.1
BC050713 mRNA Translation: AAH50713.1
CCDSiCCDS34967.1 [Q9UK41-2]
CCDS6425.1 [Q9UK41-1]
RefSeqiNP_057292.1, NM_016208.3 [Q9UK41-1]
NP_898880.1, NM_183057.2 [Q9UK41-2]
UniGeneiHs.418175
Hs.650693

Genome annotation databases

EnsembliENST00000292510; ENSP00000292510; ENSG00000160948 [Q9UK41-1]
ENST00000377348; ENSP00000366565; ENSG00000160948 [Q9UK41-2]
ENST00000526054; ENSP00000434064; ENSG00000160948 [Q9UK41-1]
ENST00000529182; ENSP00000434556; ENSG00000160948 [Q9UK41-2]
ENST00000642202; ENSP00000494521; ENSG00000285339 [Q9UK41-2]
ENST00000642867; ENSP00000496519; ENSG00000285339 [Q9UK41-1]
ENST00000643186; ENSP00000496693; ENSG00000285339 [Q9UK41-1]
ENST00000646588; ENSP00000495408; ENSG00000285339 [Q9UK41-2]
GeneIDi51160
KEGGihsa:51160
UCSCiuc003zcs.2 human [Q9UK41-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVPS28_HUMAN
AccessioniPrimary (citable) accession number: Q9UK41
Secondary accession number(s): Q86VK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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