UniProtKB - Q9UK39 (NOCT_HUMAN)
Nocturnin
NOCT
Functioni
Phosphatase which catalyzes the conversion of NADP+ to NAD+ and of NADPH to NADH (PubMed:31147539).
Shows a small preference for NADPH over NADP+ (PubMed:31147539).
Represses translation and promotes degradation of target mRNA molecules (PubMed:29860338).
Plays an important role in post-transcriptional regulation of metabolic genes under circadian control (By similarity).
Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis (By similarity).
Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance (By similarity).
Promotes adipogenesis by facilitating PPARG nuclear translocation which activates its transcriptional activity (By similarity).
Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone (By similarity).
Critical for proper development of early embryos (By similarity).
By similarity2 PublicationsCaution
Catalytic activityi
- EC:3.1.3.1081 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.1.3.1081 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Kineticsi
- KM=173 µM for NADPH1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 195 | MagnesiumCombined sources3 Publications | 1 | |
Binding sitei | 195 | Substrate1 Publication | 1 | |
Binding sitei | 263 | Substrate1 Publication | 1 | |
Binding sitei | 414 | Substrate1 Publication | 1 |
GO - Molecular functioni
- 3'-5'-exoribonuclease activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: UniProtKB
- NADPH phosphatase activity Source: UniProtKB
- NADP phosphatase activity Source: UniProtKB
- poly(A)-specific ribonuclease activity Source: UniProtKB
GO - Biological processi
- circadian regulation of gene expression Source: UniProtKB
- deadenylation-dependent decapping of nuclear-transcribed mRNA Source: Ensembl
- mRNA stabilization Source: UniProtKB
- NADP metabolic process Source: UniProtKB
- negative regulation of gene expression Source: UniProtKB
- negative regulation of osteoblast differentiation Source: UniProtKB
- P-body assembly Source: Ensembl
- positive regulation of fat cell differentiation Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- regulation of embryonic development Source: Ensembl
- response to extracellular stimulus Source: UniProtKB
- response to lipopolysaccharide Source: UniProtKB
- transcription by RNA polymerase II Source: ProtInc
Keywordsi
Molecular function | Hydrolase, Repressor, RNA-binding |
Biological process | Biological rhythms |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.108, 2681 |
PathwayCommonsi | Q9UK39 |
Reactomei | R-HSA-1368108, BMAL1:CLOCK,NPAS2 activates circadian gene expression |
SignaLinki | Q9UK39 |
Names & Taxonomyi
Protein namesi | Recommended name: NocturninImported (EC:3.1.3.1081 Publication)Alternative name(s): Carbon catabolite repression 4-like protein |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14254, NOCT |
MIMi | 608468, gene |
neXtProti | NX_Q9UK39 |
VEuPathDBi | HostDB:ENSG00000151014 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- perinuclear region By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- P-body Source: Ensembl
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 149 | N → A: Slightly decreased activity as transcriptional repressor. 1 Publication | 1 | |
Mutagenesisi | 160 | D → A: Lack of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 195 | E → A: Slightly increased activity as transcriptional repressor. Lack of catalytic activity. 2 Publications | 1 | |
Mutagenesisi | 219 | K → A: Reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 286 | H → A: No effect on activity as transcriptional repressor. 1 Publication | 1 | |
Mutagenesisi | 286 | H → N: Lack of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 288 | K → A: Reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 290 | R → A: Lack of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 324 | D → A: No effect on activity as transcriptional repressor. 1 Publication | 1 | |
Mutagenesisi | 326 | N → A: No effect on activity as transcriptional repressor. 1 Publication | 1 | |
Mutagenesisi | 365 | K → A: No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 367 | R → A: Reduced catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 377 | D → A: Slightly decreased activity as transcriptional repressor. 1 Publication | 1 | |
Mutagenesisi | 414 | H → A or N: Decreased activity as transcriptional repressor. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 25819 |
OpenTargetsi | ENSG00000151014 |
PharmGKBi | PA26176 |
Miscellaneous databases
Pharosi | Q9UK39, Tbio |
Genetic variation databases
BioMutai | NOCT |
DMDMi | 212276446 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 75 | MitochondrionSequence analysisAdd BLAST | 75 | |
ChainiPRO_0000218568 | 76 – 431 | NocturninSequence analysisAdd BLAST | 356 |
Proteomic databases
EPDi | Q9UK39 |
jPOSTi | Q9UK39 |
MassIVEi | Q9UK39 |
MaxQBi | Q9UK39 |
PaxDbi | Q9UK39 |
PeptideAtlasi | Q9UK39 |
PRIDEi | Q9UK39 |
ProteomicsDBi | 84714 |
PTM databases
iPTMneti | Q9UK39 |
PhosphoSitePlusi | Q9UK39 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000151014, Expressed in bone marrow and 148 other tissues |
ExpressionAtlasi | Q9UK39, baseline and differential |
Genevisiblei | Q9UK39, HS |
Organism-specific databases
HPAi | ENSG00000151014, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Interacts with PPARG.
By similarityProtein-protein interaction databases
BioGRIDi | 117347, 74 interactors |
IntActi | Q9UK39, 28 interactors |
STRINGi | 9606.ENSP00000280614 |
Miscellaneous databases
RNActi | Q9UK39, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9UK39 |
SMRi | Q9UK39 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 41 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 219 – 221 | Substrate binding1 Publication | 3 | |
Regioni | 286 – 289 | Substrate binding1 Publication | 4 | |
Regioni | 324 – 326 | Substrate binding1 Publication | 3 | |
Regioni | 343 – 353 | Interaction with PPARGBy similarityAdd BLAST | 11 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0620, Eukaryota |
GeneTreei | ENSGT00940000155249 |
HOGENOMi | CLU_016428_1_2_1 |
InParanoidi | Q9UK39 |
OMAi | FRLKRDC |
OrthoDBi | 1387401at2759 |
PhylomeDBi | Q9UK39 |
TreeFami | TF323175 |
Family and domain databases
CDDi | cd09096, Deadenylase_nocturnin, 1 hit |
Gene3Di | 3.60.10.10, 1 hit |
InterProi | View protein in InterPro IPR034965, Deadenylase_nocturnin IPR036691, Endo/exonu/phosph_ase_sf IPR005135, Endo/exonuclease/phosphatase IPR040109, Nocturnin |
PANTHERi | PTHR12121:SF45, PTHR12121:SF45, 1 hit |
Pfami | View protein in Pfam PF03372, Exo_endo_phos, 1 hit |
SUPFAMi | SSF56219, SSF56219, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFHSPRRLCS ALLQRDAPGL RRLPAPGLRR PLSPPAAVPR PASPRLLAAA
60 70 80 90 100
SAASGAARSC SRTVCSMGTG TSRLYSALAK TLNSSAASQH PEYLVSPDPE
110 120 130 140 150
HLEPIDPKEL LEECRAVLHT RPPRFQRDFV DLRTDCPSTH PPIRVMQWNI
160 170 180 190 200
LAQALGEGKD NFVQCPVEAL KWEERKCLIL EEILAYQPDI LCLQEVDHYF
210 220 230 240 250
DTFQPLLSRL GYQGTFFPKP WSPCLDVEHN NGPDGCALFF LQNRFKLVNS
260 270 280 290 300
ANIRLTAMTL KTNQVAIAQT LECKESGRQF CIAVTHLKAR TGWERFRSAQ
310 320 330 340 350
GCDLLQNLQN ITQGAKIPLI VCGDFNAEPT EEVYKHFASS SLNLNSAYKL
360 370 380 390 400
LSADGQSEPP YTTWKIRTSG ECRHTLDYIW YSKHALNVRS ALDLLTEEQI
410 420 430
GPNRLPSFNY PSDHLSLVCD FSFTEESDGL S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8WTX0 | Q8WTX0_HUMAN | CCR4 carbon catabolite repression 4... | NOCT CCRN4L, hCG_1735627 | 167 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | D → G in AAD56548 (PubMed:10521507).Curated | 1 | |
Sequence conflicti | 69 | T → N in AAG01387 (Ref. 4) Curated | 1 | |
Sequence conflicti | 77 | A → G in AAD56548 (PubMed:10521507).Curated | 1 | |
Sequence conflicti | 266 | A → T in AAG01389 (Ref. 4) Curated | 1 | |
Sequence conflicti | 341 | S → N in AAD56548 (PubMed:10521507).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047096 | 140 | H → Y. Corresponds to variant dbSNP:rs2271777Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF183961 mRNA Translation: AAD56548.1 CH471056 Genomic DNA Translation: EAX05129.1 CH471056 Genomic DNA Translation: EAX05131.1 BC113494 mRNA Translation: AAI13495.1 BC113500 mRNA Translation: AAI13501.1 AF199492 Genomic DNA Translation: AAG01387.1 AF199493 mRNA Translation: AAG01388.1 AF199494 Transcribed RNA Translation: AAG01389.1 |
CCDSi | CCDS3743.1 |
RefSeqi | NP_036250.2, NM_012118.3 |
Genome annotation databases
Ensembli | ENST00000280614.4; ENSP00000280614.2; ENSG00000151014.6 |
GeneIDi | 25819 |
KEGGi | hsa:25819 |
MANE-Selecti | ENST00000280614.4; ENSP00000280614.2; NM_012118.4; NP_036250.2 |
UCSCi | uc003ihl.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF183961 mRNA Translation: AAD56548.1 CH471056 Genomic DNA Translation: EAX05129.1 CH471056 Genomic DNA Translation: EAX05131.1 BC113494 mRNA Translation: AAI13495.1 BC113500 mRNA Translation: AAI13501.1 AF199492 Genomic DNA Translation: AAG01387.1 AF199493 mRNA Translation: AAG01388.1 AF199494 Transcribed RNA Translation: AAG01389.1 |
CCDSi | CCDS3743.1 |
RefSeqi | NP_036250.2, NM_012118.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6BT1 | X-ray | 1.48 | A | 120-431 | [»] | |
6BT2 | X-ray | 2.41 | A/B | 120-431 | [»] | |
6MAL | X-ray | 2.60 | A | 122-431 | [»] | |
6NF0 | X-ray | 2.70 | A | 122-431 | [»] | |
AlphaFoldDBi | Q9UK39 | |||||
SMRi | Q9UK39 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117347, 74 interactors |
IntActi | Q9UK39, 28 interactors |
STRINGi | 9606.ENSP00000280614 |
PTM databases
iPTMneti | Q9UK39 |
PhosphoSitePlusi | Q9UK39 |
Genetic variation databases
BioMutai | NOCT |
DMDMi | 212276446 |
Proteomic databases
EPDi | Q9UK39 |
jPOSTi | Q9UK39 |
MassIVEi | Q9UK39 |
MaxQBi | Q9UK39 |
PaxDbi | Q9UK39 |
PeptideAtlasi | Q9UK39 |
PRIDEi | Q9UK39 |
ProteomicsDBi | 84714 |
Protocols and materials databases
Antibodypediai | 16153, 127 antibodies from 30 providers |
DNASUi | 25819 |
Genome annotation databases
Ensembli | ENST00000280614.4; ENSP00000280614.2; ENSG00000151014.6 |
GeneIDi | 25819 |
KEGGi | hsa:25819 |
MANE-Selecti | ENST00000280614.4; ENSP00000280614.2; NM_012118.4; NP_036250.2 |
UCSCi | uc003ihl.5, human |
Organism-specific databases
CTDi | 25819 |
DisGeNETi | 25819 |
GeneCardsi | NOCT |
HGNCi | HGNC:14254, NOCT |
HPAi | ENSG00000151014, Tissue enhanced (bone) |
MIMi | 608468, gene |
neXtProti | NX_Q9UK39 |
OpenTargetsi | ENSG00000151014 |
PharmGKBi | PA26176 |
VEuPathDBi | HostDB:ENSG00000151014 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0620, Eukaryota |
GeneTreei | ENSGT00940000155249 |
HOGENOMi | CLU_016428_1_2_1 |
InParanoidi | Q9UK39 |
OMAi | FRLKRDC |
OrthoDBi | 1387401at2759 |
PhylomeDBi | Q9UK39 |
TreeFami | TF323175 |
Enzyme and pathway databases
BRENDAi | 3.1.3.108, 2681 |
PathwayCommonsi | Q9UK39 |
Reactomei | R-HSA-1368108, BMAL1:CLOCK,NPAS2 activates circadian gene expression |
SignaLinki | Q9UK39 |
Miscellaneous databases
BioGRID-ORCSi | 25819, 15 hits in 1067 CRISPR screens |
ChiTaRSi | NOCT, human |
GenomeRNAii | 25819 |
Pharosi | Q9UK39, Tbio |
PROi | PR:Q9UK39 |
RNActi | Q9UK39, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000151014, Expressed in bone marrow and 148 other tissues |
ExpressionAtlasi | Q9UK39, baseline and differential |
Genevisiblei | Q9UK39, HS |
Family and domain databases
CDDi | cd09096, Deadenylase_nocturnin, 1 hit |
Gene3Di | 3.60.10.10, 1 hit |
InterProi | View protein in InterPro IPR034965, Deadenylase_nocturnin IPR036691, Endo/exonu/phosph_ase_sf IPR005135, Endo/exonuclease/phosphatase IPR040109, Nocturnin |
PANTHERi | PTHR12121:SF45, PTHR12121:SF45, 1 hit |
Pfami | View protein in Pfam PF03372, Exo_endo_phos, 1 hit |
SUPFAMi | SSF56219, SSF56219, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NOCT_HUMAN | |
Accessioni | Q9UK39Primary (citable) accession number: Q9UK39 Secondary accession number(s): D3DNY5 Q9HD95 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | November 4, 2008 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families