UniProtKB - Q9UJY4 (GGA2_HUMAN)
ADP-ribosylation factor-binding protein GGA2
GGA2
Functioni
GO - Molecular functioni
- ADP-ribosylation factor binding Source: UniProtKB
GO - Biological processi
- cellular protein metabolic process Source: Reactome
- Golgi to plasma membrane protein transport Source: UniProtKB
- intracellular protein transport Source: UniProtKB
- protein localization to cell surface Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9UJY4 |
Reactomei | R-HSA-8854214, TBC/RABGAPs R-HSA-977225, Amyloid fiber formation |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor-binding protein GGA2Alternative name(s): Gamma-adaptin-related protein 2 Golgi-localized, gamma ear-containing, ARF-binding protein 2 VHS domain and ear domain of gamma-adaptin Short name: Vear |
Gene namesi | Name:GGA2 Synonyms:KIAA1080 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16064, GGA2 |
MIMi | 606005, gene |
neXtProti | NX_Q9UJY4 |
VEuPathDBi | HostDB:ENSG00000103365.15 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network membrane 3 Publications; Peripheral membrane protein 2 Publications
Endosome
- Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication
- Early endosome membrane 1 Publication; Peripheral membrane protein Curated
Endosome
- early endosome membrane Source: Reactome
- endosome membrane Source: Reactome
Golgi apparatus
- Golgi apparatus Source: HPA
- trans-Golgi network Source: UniProtKB
Other locations
- clathrin-coated vesicle Source: UniProtKB
Keywords - Cellular componenti
Endosome, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 349 – 353 | LIDLE → AADAA: Partial loss of clathrin-binding. 1 Publication | 5 |
Organism-specific databases
DisGeNETi | 23062 |
OpenTargetsi | ENSG00000103365 |
PharmGKBi | PA28658 |
Miscellaneous databases
Pharosi | Q9UJY4, Tbio |
Genetic variation databases
BioMutai | GGA2 |
DMDMi | 143811397 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212682 | 1 – 613 | ADP-ribosylation factor-binding protein GGA2Add BLAST | 613 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 400 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9UJY4 |
jPOSTi | Q9UJY4 |
MassIVEi | Q9UJY4 |
MaxQBi | Q9UJY4 |
PaxDbi | Q9UJY4 |
PeptideAtlasi | Q9UJY4 |
PRIDEi | Q9UJY4 |
ProteomicsDBi | 84691 |
TopDownProteomicsi | Q9UJY4 |
PTM databases
iPTMneti | Q9UJY4 |
PhosphoSitePlusi | Q9UJY4 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000103365, Expressed in tonsil and 236 other tissues |
ExpressionAtlasi | Q9UJY4, baseline and differential |
Genevisiblei | Q9UJY4, HS |
Organism-specific databases
HPAi | ENSG00000103365, Low tissue specificity |
Interactioni
Subunit structurei
Monomer (Probable).
Interacts with NECAP1, TSG101, UBC and AFTPH/aftiphilin.
Interacts with CNST (By similarity).
Interacts with GGA1 and GGA3 (PubMed:14638859). Binds to clathrin and activated ARFs, such as ARF1, ARF5 and ARF6 (PubMed:11301005, PubMed:11950392). Binds RABEP1 and RABGEF1 (PubMed:12505986).
Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1 (PubMed:11390366, PubMed:11387475, PubMed:11886874, PubMed:14567678).
Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (PubMed:11331584, PubMed:11390366, PubMed:11821067). Binds the accessory proteins CCDC91, P200, SYNRG, EPN4 and NECAP2 (PubMed:10814529, PubMed:12429846, PubMed:12808037, PubMed:14665628).
Interacts with ADRA2B (PubMed:27901063).
Interacts (via VHS domain) with PIK4B; the interaction is important for PIK4B location at the Golgi apparatus membrane (PubMed:28289207).
By similarityCurated16 PublicationsBinary interactionsi
Hide detailsQ9UJY4
GO - Molecular functioni
- ADP-ribosylation factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116697, 46 interactors |
DIPi | DIP-31601N |
ELMi | Q9UJY4 |
IntActi | Q9UJY4, 32 interactors |
MINTi | Q9UJY4 |
STRINGi | 9606.ENSP00000311962 |
Miscellaneous databases
RNActi | Q9UJY4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UJY4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UJY4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 163 | VHSPROSITE-ProRule annotationAdd BLAST | 131 | |
Domaini | 188 – 315 | GATPROSITE-ProRule annotationAdd BLAST | 128 | |
Domaini | 484 – 605 | GAEPROSITE-ProRule annotationAdd BLAST | 122 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 316 – 483 | Unstructured hingeAdd BLAST | 168 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1086, Eukaryota |
GeneTreei | ENSGT00940000159613 |
HOGENOMi | CLU_015010_0_0_1 |
InParanoidi | Q9UJY4 |
OMAi | KVAGQNC |
OrthoDBi | 594067at2759 |
PhylomeDBi | Q9UJY4 |
TreeFami | TF318574 |
Family and domain databases
Gene3Di | 1.20.58.160, 1 hit 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008942, ENTH_VHS IPR008153, GAE_dom IPR004152, GAT_dom IPR038425, GAT_sf IPR041198, GGA_N-GAT IPR002014, VHS_dom |
Pfami | View protein in Pfam PF02883, Alpha_adaptinC2, 1 hit PF03127, GAT, 1 hit PF18308, GGA_N-GAT, 1 hit PF00790, VHS, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit SM00288, VHS, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit PS50909, GAT, 1 hit PS50179, VHS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAATAVAAAV AGTESAQGPP GPAASLELWL NKATDPSMSE QDWSAIQNFC
60 70 80 90 100
EQVNTDPNGP THAPWLLAHK IQSPQEKEAL YALTVLEMCM NHCGEKFHSE
110 120 130 140 150
VAKFRFLNEL IKVLSPKYLG SWATGKVKGR VIEILFSWTV WFPEDIKIRD
160 170 180 190 200
AYQMLKKQGI IKQDPKLPVD KILPPPSPWP KSSIFDADEE KSKLLTRLLK
210 220 230 240 250
SNHPEDLQAA NRLIKNLVKE EQEKSEKVSK RVSAVEEVRS HVKVLQEMLS
260 270 280 290 300
MYRRPGQAPP DQEALQVVYE RCEKLRPTLF RLASDTTDDD DALAEILQAN
310 320 330 340 350
DLLTQGVLLY KQVMEGRVTF GNRVTSSLGD IPVSRVFQNP AGCMKTCPLI
360 370 380 390 400
DLEVDNGPAQ MGTVVPSLLH QDLAALGISD APVTGMVSGQ NCCEEKRNPS
410 420 430 440 450
SSTLPGGGVQ NPSADRNLLD LLSAQPAPCP LNYVSQKSVP KEVPPGTKSS
460 470 480 490 500
PGWSWEAGPL APSPSSQNTP LAQVFVPLES VKPSSLPPLI VYDRNGFRIL
510 520 530 540 550
LHFSQTGAPG HPEVQVLLLT MMSTAPQPVW DIMFQVAVPK SMRVKLQPAS
560 570 580 590 600
SSKLPAFSPL MPPAVISQML LLDNPHKEPI RLRYKLTFNQ GGQPFSEVGE
610
VKDFPDLAVL GAA
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L263 | I3L263_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 129 | Annotation score: | ||
I3L2C4 | I3L2C4_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 117 | Annotation score: | ||
H3BMN6 | H3BMN6_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 59 | Annotation score: | ||
H3BMM6 | H3BMM6_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 223 | Annotation score: | ||
H3BPI3 | H3BPI3_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 125 | Annotation score: | ||
H3BPF4 | H3BPF4_HUMAN | ADP-ribosylation factor-binding pro... | GGA2 | 49 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 281 | R → W in AAF05708 (PubMed:10749927).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_028275 | 424 | A → P4 PublicationsCorresponds to variant dbSNP:rs1135045Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190863 mRNA Translation: AAF05708.1 AF233522 mRNA Translation: AAF35394.1 AF165531 mRNA Translation: AAF42806.1 AC002400 Genomic DNA Translation: AAC05813.1 CH471145 Genomic DNA Translation: EAW55827.1 CH471145 Genomic DNA Translation: EAW55828.1 BC000284 mRNA Translation: AAH00284.1 AF323754 mRNA Translation: AAK38634.1 Frameshift. AB029003 mRNA Translation: BAA83032.1 |
CCDSi | CCDS10611.1 |
PIRi | T00744 |
RefSeqi | NP_055859.1, NM_015044.4 |
Genome annotation databases
Ensembli | ENST00000309859; ENSP00000311962; ENSG00000103365 |
GeneIDi | 23062 |
KEGGi | hsa:23062 |
UCSCi | uc002dlq.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190863 mRNA Translation: AAF05708.1 AF233522 mRNA Translation: AAF35394.1 AF165531 mRNA Translation: AAF42806.1 AC002400 Genomic DNA Translation: AAC05813.1 CH471145 Genomic DNA Translation: EAW55827.1 CH471145 Genomic DNA Translation: EAW55828.1 BC000284 mRNA Translation: AAH00284.1 AF323754 mRNA Translation: AAK38634.1 Frameshift. AB029003 mRNA Translation: BAA83032.1 |
CCDSi | CCDS10611.1 |
PIRi | T00744 |
RefSeqi | NP_055859.1, NM_015044.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MHQ | X-ray | 2.20 | A/B | 25-172 | [»] | |
SMRi | Q9UJY4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116697, 46 interactors |
DIPi | DIP-31601N |
ELMi | Q9UJY4 |
IntActi | Q9UJY4, 32 interactors |
MINTi | Q9UJY4 |
STRINGi | 9606.ENSP00000311962 |
PTM databases
iPTMneti | Q9UJY4 |
PhosphoSitePlusi | Q9UJY4 |
Genetic variation databases
BioMutai | GGA2 |
DMDMi | 143811397 |
Proteomic databases
EPDi | Q9UJY4 |
jPOSTi | Q9UJY4 |
MassIVEi | Q9UJY4 |
MaxQBi | Q9UJY4 |
PaxDbi | Q9UJY4 |
PeptideAtlasi | Q9UJY4 |
PRIDEi | Q9UJY4 |
ProteomicsDBi | 84691 |
TopDownProteomicsi | Q9UJY4 |
Protocols and materials databases
Antibodypediai | 12586, 202 antibodies |
DNASUi | 23062 |
Genome annotation databases
Ensembli | ENST00000309859; ENSP00000311962; ENSG00000103365 |
GeneIDi | 23062 |
KEGGi | hsa:23062 |
UCSCi | uc002dlq.3, human |
Organism-specific databases
CTDi | 23062 |
DisGeNETi | 23062 |
GeneCardsi | GGA2 |
HGNCi | HGNC:16064, GGA2 |
HPAi | ENSG00000103365, Low tissue specificity |
MIMi | 606005, gene |
neXtProti | NX_Q9UJY4 |
OpenTargetsi | ENSG00000103365 |
PharmGKBi | PA28658 |
VEuPathDBi | HostDB:ENSG00000103365.15 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1086, Eukaryota |
GeneTreei | ENSGT00940000159613 |
HOGENOMi | CLU_015010_0_0_1 |
InParanoidi | Q9UJY4 |
OMAi | KVAGQNC |
OrthoDBi | 594067at2759 |
PhylomeDBi | Q9UJY4 |
TreeFami | TF318574 |
Enzyme and pathway databases
PathwayCommonsi | Q9UJY4 |
Reactomei | R-HSA-8854214, TBC/RABGAPs R-HSA-977225, Amyloid fiber formation |
Miscellaneous databases
BioGRID-ORCSi | 23062, 3 hits in 867 CRISPR screens |
ChiTaRSi | GGA2, human |
EvolutionaryTracei | Q9UJY4 |
GeneWikii | GGA2 |
GenomeRNAii | 23062 |
Pharosi | Q9UJY4, Tbio |
PROi | PR:Q9UJY4 |
RNActi | Q9UJY4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103365, Expressed in tonsil and 236 other tissues |
ExpressionAtlasi | Q9UJY4, baseline and differential |
Genevisiblei | Q9UJY4, HS |
Family and domain databases
Gene3Di | 1.20.58.160, 1 hit 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008942, ENTH_VHS IPR008153, GAE_dom IPR004152, GAT_dom IPR038425, GAT_sf IPR041198, GGA_N-GAT IPR002014, VHS_dom |
Pfami | View protein in Pfam PF02883, Alpha_adaptinC2, 1 hit PF03127, GAT, 1 hit PF18308, GGA_N-GAT, 1 hit PF00790, VHS, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit SM00288, VHS, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit PS50909, GAT, 1 hit PS50179, VHS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GGA2_HUMAN | |
Accessioni | Q9UJY4Primary (citable) accession number: Q9UJY4 Secondary accession number(s): D3DWF0 Q9UPS2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | April 3, 2007 | |
Last modified: | February 10, 2021 | |
This is version 195 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families