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Protein

Cell division cycle protein 23 homolog

Gene

CDC23

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: Reactome
  • cell division Source: UniProtKB-KW
  • metaphase/anaphase transition of mitotic cell cycle Source: ProtInc
  • mitotic cell cycle Source: UniProtKB
  • mitotic metaphase plate congression Source: UniProtKB
  • protein K11-linked ubiquitination Source: UniProtKB
  • regulation of exit from mitosis Source: ProtInc
  • regulation of mitotic metaphase/anaphase transition Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: ProtInc

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiQ9UJX2
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 23 homolog
Alternative name(s):
Anaphase-promoting complex subunit 8
Short name:
APC8
Cyclosome subunit 8
Gene namesi
Name:CDC23
Synonyms:ANAPC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000094880.10
HGNCiHGNC:1724 CDC23
MIMi603462 gene
neXtProtiNX_Q9UJX2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi8697
OpenTargetsiENSG00000094880
PharmGKBiPA26258

Polymorphism and mutation databases

BioMutaiCDC23
DMDMi254763423

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001062702 – 597Cell division cycle protein 23 homologAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei273Phosphotyrosine1 Publication1
Modified residuei467N6-acetyllysineCombined sources1
Modified residuei562PhosphothreonineCombined sources1 Publication1
Modified residuei565Phosphothreonine1 Publication1
Modified residuei578PhosphoserineCombined sources1
Modified residuei582PhosphothreonineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei593PhosphoserineCombined sources1
Modified residuei596PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UJX2
PaxDbiQ9UJX2
PeptideAtlasiQ9UJX2
PRIDEiQ9UJX2
ProteomicsDBi84679
84680 [Q9UJX2-2]
84681 [Q9UJX2-3]

PTM databases

iPTMnetiQ9UJX2
PhosphoSitePlusiQ9UJX2

Expressioni

Gene expression databases

BgeeiENSG00000094880
CleanExiHS_CDC23
ExpressionAtlasiQ9UJX2 baseline and differential
GenevisibleiQ9UJX2 HS

Organism-specific databases

HPAiHPA039593

Interactioni

Subunit structurei

The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114242, 134 interactors
CORUMiQ9UJX2
DIPiDIP-32959N
ELMiQ9UJX2
IntActiQ9UJX2, 139 interactors
MINTiQ9UJX2
STRINGi9606.ENSP00000378350

Structurei

Secondary structure

1597
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 46Combined sources18
Helixi49 – 61Combined sources13
Beta strandi67 – 69Combined sources3
Helixi78 – 95Combined sources18
Helixi99 – 105Combined sources7
Helixi112 – 136Combined sources25
Turni140 – 142Combined sources3
Beta strandi143 – 145Combined sources3
Helixi151 – 164Combined sources14
Helixi170 – 181Combined sources12
Helixi185 – 198Combined sources14
Helixi203 – 212Combined sources10
Helixi216 – 221Combined sources6
Helixi229 – 241Combined sources13
Helixi244 – 256Combined sources13
Helixi259 – 261Combined sources3
Helixi263 – 275Combined sources13
Helixi279 – 292Combined sources14
Helixi300 – 309Combined sources10
Helixi313 – 326Combined sources14
Beta strandi328 – 330Combined sources3
Helixi331 – 343Combined sources13
Helixi347 – 360Combined sources14
Beta strandi361 – 363Combined sources3
Helixi365 – 378Combined sources14
Helixi381 – 394Combined sources14
Helixi399 – 412Combined sources14
Helixi415 – 428Combined sources14
Helixi433 – 446Combined sources14
Helixi449 – 462Combined sources14
Helixi468 – 479Combined sources12
Helixi483 – 499Combined sources17
Helixi510 – 523Combined sources14
Helixi526 – 538Combined sources13
Helixi542 – 556Combined sources15

3D structure databases

ProteinModelPortaliQ9UJX2
SMRiQ9UJX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 63TPR 1Add BLAST37
Repeati73 – 112TPR 2Add BLAST40
Repeati114 – 144TPR 3Add BLAST31
Repeati169 – 200TPR 4Add BLAST32
Repeati229 – 259TPR 5Add BLAST31
Repeati263 – 293TPR 6Add BLAST31
Repeati297 – 327TPR 7Add BLAST31
Repeati331 – 361TPR 8Add BLAST31
Repeati366 – 395TPR 9Add BLAST30
Repeati400 – 432TPR 10Add BLAST33
Repeati433 – 466TPR 11Add BLAST34
Repeati468 – 500TPR 12Add BLAST33
Repeati504 – 540TPR 13Add BLAST37

Sequence similaritiesi

Belongs to the APC8/CDC23 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1155 Eukaryota
ENOG410XPS3 LUCA
GeneTreeiENSGT00550000074886
HOVERGENiHBG050858
InParanoidiQ9UJX2
KOiK03355
OMAiMGHEFME
OrthoDBiEOG091G0535
PhylomeDBiQ9UJX2
TreeFamiTF101055

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR007192 APC8
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF04049 ANAPC8, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 7 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASTSMVPV AVTAAVAPVL SINSDFSDLR EIKKQLLLIA GLTRERGLLH
60 70 80 90 100
SSKWSAELAF SLPALPLAEL QPPPPITEED AQDMDAYTLA KAYFDVKEYD
110 120 130 140 150
RAAHFLHGCN SKKAYFLYMY SRYLSGEKKK DDETVDSLGP LEKGQVKNEA
160 170 180 190 200
LRELRVELSK KHQARELDGF GLYLYGVVLR KLDLVKEAID VFVEATHVLP
210 220 230 240 250
LHWGAWLELC NLITDKEMLK FLSLPDTWMK EFFLAHIYTE LQLIEEALQK
260 270 280 290 300
YQNLIDVGFS KSSYIVSQIA VAYHNIRDID KALSIFNELR KQDPYRIENM
310 320 330 340 350
DTFSNLLYVR SMKSELSYLA HNLCEIDKYR VETCCVIGNY YSLRSQHEKA
360 370 380 390 400
ALYFQRALKL NPRYLGAWTL MGHEYMEMKN TSAAIQAYRH AIEVNKRDYR
410 420 430 440 450
AWYGLGQTYE ILKMPFYCLY YYRRAHQLRP NDSRMLVALG ECYEKLNQLV
460 470 480 490 500
EAKKCYWRAY AVGDVEKMAL VKLAKLHEQL TESEQAAQCY IKYIQDIYSC
510 520 530 540 550
GEIVEHLEES TAFRYLAQYY FKCKLWDEAS TCAQKCCAFN DTREEGKALL
560 570 580 590
RQILQLRNQG ETPTTEVPAP FFLPASLSAN NTPTRRVSPL NLSSVTP
Length:597
Mass (Da):68,834
Last modified:July 28, 2009 - v3
Checksum:i358F2B8745DB9D32
GO
Isoform 2 (identifier: Q9UJX2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-151: SGEKKKDDETVDSLGPLEKGQVKNEAL → VRAILKCHSAFSETSIFRTNGKVKSFK
     152-597: Missing.

Note: No experimental confirmation available.
Show »
Length:151
Mass (Da):16,821
Checksum:i0FE42406F299DAD4
GO
Isoform 3 (identifier: Q9UJX2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Note: No experimental confirmation available.
Show »
Length:479
Mass (Da):55,882
Checksum:i1E4655ABEAAB2D9A
GO

Sequence cautioni

The sequence AAC70920 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH05258 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH10944 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH17713 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS99353 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA75628 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD96970 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAF84299 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83D → E in BAD96970 (Ref. 4) Curated1
Sequence conflicti105F → S in BAD96970 (Ref. 4) Curated1
Sequence conflicti527D → G in BAG65414 (PubMed:14702039).Curated1
Sequence conflicti588S → F in AAF05755 (PubMed:9469815).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0246759P → L. Corresponds to variant dbSNP:rs2231471Ensembl.1
Natural variantiVAR_01923278E → Q1 PublicationCorresponds to variant dbSNP:rs17228304Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376781 – 118Missing in isoform 3. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_008429125 – 151SGEKK…KNEAL → VRAILKCHSAFSETSIFRTN GKVKSFK in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_008430152 – 597Missing in isoform 2. 1 PublicationAdd BLAST446

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011472 mRNA Translation: BAA75628.1 Different initiation.
AK291610 mRNA Translation: BAF84299.1 Different initiation.
AK304635 mRNA Translation: BAG65414.1
AY603103 Genomic DNA Translation: AAS99353.1 Different initiation.
AK223250 mRNA Translation: BAD96970.1 Different initiation.
AC106752 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW62155.1
CH471062 Genomic DNA Translation: EAW62154.1
CH471062 Genomic DNA Translation: EAW62156.1
BC005258 mRNA Translation: AAH05258.1 Different initiation.
BC010944 mRNA Translation: AAH10944.1 Different initiation.
BC017713 mRNA Translation: AAH17713.1 Different initiation.
AF053977 mRNA Translation: AAC70920.1 Different initiation.
AF191341 mRNA Translation: AAF05755.1
BT009810 mRNA Translation: AAP88812.1
CCDSiCCDS4200.2 [Q9UJX2-1]
PIRiT51168
RefSeqiNP_004652.2, NM_004661.3 [Q9UJX2-1]
UniGeneiHs.73625

Genome annotation databases

EnsembliENST00000394884; ENSP00000378348; ENSG00000094880 [Q9UJX2-2]
ENST00000394886; ENSP00000378350; ENSG00000094880 [Q9UJX2-1]
GeneIDi8697
KEGGihsa:8697
UCSCiuc003lcl.3 human [Q9UJX2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCDC23_HUMAN
AccessioniPrimary (citable) accession number: Q9UJX2
Secondary accession number(s): A8K6E5
, B4E3A2, B7WP05, D3DQB7, O75433, Q53FN2, Q9BS73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: July 28, 2009
Last modified: July 18, 2018
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

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