UniProtKB - Q9UJW0 (DCTN4_HUMAN)
Dynactin subunit 4
DCTN4
Functioni
GO - Molecular functioni
- protein N-terminus binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Enzyme and pathway databases
PathwayCommonsi | Q9UJW0 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Dynactin subunit 4Short name: Dyn4 Alternative name(s): Dynactin subunit p62 |
Gene namesi | Name:DCTN4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15518, DCTN4 |
MIMi | 614758, gene |
neXtProti | NX_Q9UJW0 |
VEuPathDBi | HostDB:ENSG00000132912.12 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
- centrosome 1 Publication
- stress fiber By similarity
Other locations
- cell cortex By similarity
- sarcomere By similarity
Note: Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity).By similarity1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
- cytoplasmic dynein complex Source: Ensembl
- dynactin complex Source: GO_Central
- spindle pole Source: Ensembl
- stress fiber Source: Ensembl
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: ProtInc
Other locations
- cell cortex Source: UniProtKB-SubCell
- cytoplasm Source: ProtInc
- focal adhesion Source: Ensembl
- kinetochore Source: Ensembl
- sarcomere Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 30 | C → S: Loss of ATP7B-binding; when associated with S-33; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 33 | C → S: Loss of ATP7B-binding; when associated with S-30; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 51 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 54 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 70 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 73 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 76 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-79; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 79 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-111; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 111 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-114; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 114 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-277 and S-280. 1 Publication | 1 | |
Mutagenesisi | 277 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-280. 1 Publication | 1 | |
Mutagenesisi | 280 | C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-277. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51164 |
MalaCardsi | DCTN4 |
OpenTargetsi | ENSG00000132912 |
Orphaneti | 586, Cystic fibrosis |
PharmGKBi | PA27183 |
Miscellaneous databases
Pharosi | Q9UJW0, Tbio |
Genetic variation databases
BioMutai | DCTN4 |
DMDMi | 62900106 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000079823 | 2 – 460 | Dynactin subunit 4Add BLAST | 459 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 196 | PhosphoserineCombined sources | 1 | |
Cross-linki | 215 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 407 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9UJW0 |
jPOSTi | Q9UJW0 |
MassIVEi | Q9UJW0 |
PaxDbi | Q9UJW0 |
PeptideAtlasi | Q9UJW0 |
PRIDEi | Q9UJW0 |
ProteomicsDBi | 84667 [Q9UJW0-1] 84668 [Q9UJW0-2] 84669 [Q9UJW0-3] |
PTM databases
iPTMneti | Q9UJW0 |
PhosphoSitePlusi | Q9UJW0 |
Expressioni
Gene expression databases
Bgeei | ENSG00000132912, Expressed in cerebral cortex and 236 other tissues |
ExpressionAtlasi | Q9UJW0, baseline and differential |
Genevisiblei | Q9UJW0, HS |
Organism-specific databases
HPAi | ENSG00000132912, Low tissue specificity |
Interactioni
Subunit structurei
Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin.
Interacts with ATP7B, but not ATP7A, in a copper-dependent manner.
Interacts with ANK2; this interaction is required for localization at costameres (By similarity).
By similarityBinary interactionsi
Hide detailsQ9UJW0
GO - Molecular functioni
- protein N-terminus binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119345, 93 interactors |
IntActi | Q9UJW0, 40 interactors |
MINTi | Q9UJW0 |
STRINGi | 9606.ENSP00000414906 |
Miscellaneous databases
RNActi | Q9UJW0, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 152 – 172 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3896, Eukaryota |
GeneTreei | ENSGT00390000006954 |
HOGENOMi | CLU_030384_0_0_1 |
InParanoidi | Q9UJW0 |
OMAi | SHFCSNC |
OrthoDBi | 853728at2759 |
PhylomeDBi | Q9UJW0 |
TreeFami | TF105249 |
Family and domain databases
InterProi | View protein in InterPro IPR008603, DCTN4 |
PANTHERi | PTHR13034, PTHR13034, 1 hit |
Pfami | View protein in Pfam PF05502, Dynactin_p62, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY
60 70 80 90 100
CPSCLENMPS AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK
110 120 130 140 150
TTMKKAYYLA CGFCRWTSRD VGMADKSVAS GGWQEPENPH TQRMNKLIEY
160 170 180 190 200
YQQLAQKEKV ERDRKKLARR RNYMPLAFSD KYGLGTRLQR PRAGASISTL
210 220 230 240 250
AGLSLKEGED QKEIKIEPAQ AVDEVEPLPE DYYTRPVNLT EVTTLQQRLL
260 270 280 290 300
QPDFQPVCAS QLYPRHKHLL IKRSLRCRKC EHNLSKPEFN PTSIKFKIQL
310 320 330 340 350
VAVNYIPEVR IMSIPNLRYM KESQVLLTLT NPVENLTHVT LFECEEGDPD
360 370 380 390 400
DINSTAKVVV PPKELVLAGK DAAAEYDELA EPQDFQDDPD IIAFRKANKV
410 420 430 440 450
GIFIKVTPQR EEGEVTVCFK MKHDFKNLAA PIRPIEESDQ GTEVIWLTQH
460
VELSLGPLLP
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH9KVE0 | H9KVE0_HUMAN | Dynactin subunit 4 | DCTN4 | 134 | Annotation score: | ||
E5RGG1 | E5RGG1_HUMAN | Dynactin subunit 4 | DCTN4 | 114 | Annotation score: | ||
E5RI97 | E5RI97_HUMAN | Dynactin subunit 4 | DCTN4 | 86 | Annotation score: | ||
E5RK21 | E5RK21_HUMAN | Dynactin subunit 4 | DCTN4 | 91 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_054037 | 95 | P → T1 PublicationCorresponds to variant dbSNP:rs11550931Ensembl. | 1 | |
Natural variantiVAR_033847 | 263 | Y → C. Corresponds to variant dbSNP:rs35772018Ensembl. | 1 | |
Natural variantiVAR_024336 | 342 | F → L1 PublicationCorresponds to variant dbSNP:rs11954652Ensembl. | 1 | |
Natural variantiVAR_024337 | 438 | S → N. Corresponds to variant dbSNP:rs3733923Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041306 | 1 – 57 | Missing in isoform 2. 1 PublicationAdd BLAST | 57 | |
Alternative sequenceiVSP_041307 | 179 | S → SQHTIHVV in isoform 3. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF195120 mRNA Translation: AAF03896.1 AK125973 mRNA Translation: BAG54272.1 AK000299 mRNA Translation: BAA91066.1 AC008450 Genomic DNA No translation available. AC008453 Genomic DNA No translation available. CH471062 Genomic DNA Translation: EAW61706.1 CH471062 Genomic DNA Translation: EAW61707.1 CH471062 Genomic DNA Translation: EAW61709.1 BC026323 mRNA Translation: AAH26323.1 |
CCDSi | CCDS4310.1 [Q9UJW0-1] CCDS47310.1 [Q9UJW0-3] CCDS47311.1 [Q9UJW0-2] |
RefSeqi | NP_001129115.1, NM_001135643.1 [Q9UJW0-3] NP_001129116.1, NM_001135644.1 [Q9UJW0-2] NP_057305.1, NM_016221.3 [Q9UJW0-1] XP_011535946.1, XM_011537644.1 [Q9UJW0-2] XP_011535947.1, XM_011537645.1 [Q9UJW0-2] |
Genome annotation databases
Ensembli | ENST00000424236; ENSP00000411251; ENSG00000132912 [Q9UJW0-2] ENST00000446090; ENSP00000414906; ENSG00000132912 [Q9UJW0-3] ENST00000447998; ENSP00000416968; ENSG00000132912 [Q9UJW0-1] |
GeneIDi | 51164 |
KEGGi | hsa:51164 |
UCSCi | uc003lsu.4, human [Q9UJW0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF195120 mRNA Translation: AAF03896.1 AK125973 mRNA Translation: BAG54272.1 AK000299 mRNA Translation: BAA91066.1 AC008450 Genomic DNA No translation available. AC008453 Genomic DNA No translation available. CH471062 Genomic DNA Translation: EAW61706.1 CH471062 Genomic DNA Translation: EAW61707.1 CH471062 Genomic DNA Translation: EAW61709.1 BC026323 mRNA Translation: AAH26323.1 |
CCDSi | CCDS4310.1 [Q9UJW0-1] CCDS47310.1 [Q9UJW0-3] CCDS47311.1 [Q9UJW0-2] |
RefSeqi | NP_001129115.1, NM_001135643.1 [Q9UJW0-3] NP_001129116.1, NM_001135644.1 [Q9UJW0-2] NP_057305.1, NM_016221.3 [Q9UJW0-1] XP_011535946.1, XM_011537644.1 [Q9UJW0-2] XP_011535947.1, XM_011537645.1 [Q9UJW0-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 119345, 93 interactors |
IntActi | Q9UJW0, 40 interactors |
MINTi | Q9UJW0 |
STRINGi | 9606.ENSP00000414906 |
PTM databases
iPTMneti | Q9UJW0 |
PhosphoSitePlusi | Q9UJW0 |
Genetic variation databases
BioMutai | DCTN4 |
DMDMi | 62900106 |
Proteomic databases
EPDi | Q9UJW0 |
jPOSTi | Q9UJW0 |
MassIVEi | Q9UJW0 |
PaxDbi | Q9UJW0 |
PeptideAtlasi | Q9UJW0 |
PRIDEi | Q9UJW0 |
ProteomicsDBi | 84667 [Q9UJW0-1] 84668 [Q9UJW0-2] 84669 [Q9UJW0-3] |
Protocols and materials databases
Antibodypediai | 4309, 213 antibodies |
DNASUi | 51164 |
Genome annotation databases
Ensembli | ENST00000424236; ENSP00000411251; ENSG00000132912 [Q9UJW0-2] ENST00000446090; ENSP00000414906; ENSG00000132912 [Q9UJW0-3] ENST00000447998; ENSP00000416968; ENSG00000132912 [Q9UJW0-1] |
GeneIDi | 51164 |
KEGGi | hsa:51164 |
UCSCi | uc003lsu.4, human [Q9UJW0-1] |
Organism-specific databases
CTDi | 51164 |
DisGeNETi | 51164 |
GeneCardsi | DCTN4 |
HGNCi | HGNC:15518, DCTN4 |
HPAi | ENSG00000132912, Low tissue specificity |
MalaCardsi | DCTN4 |
MIMi | 614758, gene |
neXtProti | NX_Q9UJW0 |
OpenTargetsi | ENSG00000132912 |
Orphaneti | 586, Cystic fibrosis |
PharmGKBi | PA27183 |
VEuPathDBi | HostDB:ENSG00000132912.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3896, Eukaryota |
GeneTreei | ENSGT00390000006954 |
HOGENOMi | CLU_030384_0_0_1 |
InParanoidi | Q9UJW0 |
OMAi | SHFCSNC |
OrthoDBi | 853728at2759 |
PhylomeDBi | Q9UJW0 |
TreeFami | TF105249 |
Enzyme and pathway databases
PathwayCommonsi | Q9UJW0 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation R-HSA-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic |
Miscellaneous databases
BioGRID-ORCSi | 51164, 371 hits in 881 CRISPR screens |
ChiTaRSi | DCTN4, human |
GenomeRNAii | 51164 |
Pharosi | Q9UJW0, Tbio |
PROi | PR:Q9UJW0 |
RNActi | Q9UJW0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132912, Expressed in cerebral cortex and 236 other tissues |
ExpressionAtlasi | Q9UJW0, baseline and differential |
Genevisiblei | Q9UJW0, HS |
Family and domain databases
InterProi | View protein in InterPro IPR008603, DCTN4 |
PANTHERi | PTHR13034, PTHR13034, 1 hit |
Pfami | View protein in Pfam PF05502, Dynactin_p62, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCTN4_HUMAN | |
Accessioni | Q9UJW0Primary (citable) accession number: Q9UJW0 Secondary accession number(s): B3KWW0 Q8TAN8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families