Lymphoid enhancer-binding factor 1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• armadillo repeat domain binding Source: BHF-UCL <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules."
    Daniels D.L., Weis W.I.
    Mol. Cell 10:573-584(2002) [PubMed] [Europe PMC] [Abstract]
• beta-catenin binding Source: BHF-UCL <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.10

    "Two members of the Tcf family implicated in Wnt/b-catenin signaling during embryogenesis in the mouse."
    Korinek V., Barker N., Willert K., Molenaar M., Roose J., Wagenaar G., Markman M., Lamers W., Destree O., Clevers H.
    Mol. Cell. Biol. 18:1248-1256(1998) [PubMed] [Europe PMC] [Abstract]

    Cited for: INTERACTION WITH CTNNB1.
• C2H2 zinc finger domain binding Source: UniProtKBInferred from physical interactionⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• chromatin binding Source: Ensembl
• DNA binding Source: UniProtKB
• DNA binding, bending Source: UniProtKB
• DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis."
    Skokowa J., Klimiankou M., Klimenkova O., Lan D., Gupta K., Hussein K., Carrizosa E., Kusnetsova I., Li Z., Sustmann C., Ganser A., Zeidler C., Kreipe H.H., Burkhardt J., Grosschedl R., Welte K.
    Nat Med 18:1550-1559(2012) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity Source: BHF-UCLInferred from direct assayⁱ
  • "Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro."
    Tutter A.V., Fryer C.J., Jones K.A.
    Genes Dev. 15:3342-3354(2001) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_CentralInferred from direct assayⁱ
  • "Nkx3-1 and LEF-1 function as transcriptional inhibitors of estrogen receptor activity."
    Holmes K.A., Song J.S., Liu X.S., Brown M., Carroll J.S.
    Cancer Res 68:7380-7385(2008) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: Ensembl
• estrogen receptor binding Source: UniProtKBInferred from direct assayⁱ
  • "Nkx3-1 and LEF-1 function as transcriptional inhibitors of estrogen receptor activity."
    Holmes K.A., Song J.S., Liu X.S., Brown M., Carroll J.S.
    Cancer Res 68:7380-7385(2008) [PubMed] [Europe PMC] [Abstract]
• gamma-catenin binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Plakoglobin (gamma-catenin) has TCF/LEF family-dependent transcriptional activity in beta-catenin-deficient cell line."
    Maeda O., Usami N., Kondo M., Takahashi M., Goto H., Shimokata K., Kusugami K., Sekido Y.
    Oncogene 23:964-972(2004) [PubMed] [Europe PMC] [Abstract]
• histone binding Source: UniProtKBInferred from physical interactionⁱ
  • "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements."
    Chen Q.Y., Zhang T., Pincus S.H., Wu S., Ricks D., Liu D., Sun Z., Maclaren N., Lan M.S.
    J Immunol 184:5047-5054(2010) [PubMed] [Europe PMC] [Abstract]
• histone deacetylase binding Source: ParkinsonsUK-UCLInferred from physical interactionⁱ
  • "Dapper1 is a nucleocytoplasmic shuttling protein that negatively modulates Wnt signaling in the nucleus."
    Gao X., Wen J., Zhang L., Li X., Ning Y., Meng A., Chen Y.G.
    J. Biol. Chem. 283:35679-35688(2008) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_CentralInferred from direct assayⁱ
  • "Differential use of functional domains by coiled-coil coactivator in its synergistic coactivator function with beta-catenin or GRIP1."
    Yang C.K., Kim J.H., Li H., Stallcup M.R.
    J. Biol. Chem. 281:3389-3397(2006) [PubMed] [Europe PMC] [Abstract]
  • "LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia."
    Skokowa J., Cario G., Uenalan M., Schambach A., Germeshausen M., Battmer K., Zeidler C., Lehmann U., Eder M., Baum C., Grosschedl R., Stanulla M., Scherr M., Welte K.
    Nat Med 12:1191-1197(2006) [PubMed] [Europe PMC] [Abstract]
  • "LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element."
    Hebenstreit D., Giaisi M., Treiber M.K., Zhang X.B., Mi H.F., Horejs-Hoeck J., Andersen K.G., Krammer P.H., Duschl A., Li-Weber M.
    J Biol Chem 283:22490-22497(2008) [PubMed] [Europe PMC] [Abstract]
  • "LEF1 in androgen-independent prostate cancer: regulation of androgen receptor expression, prostate cancer growth, and invasion."
    Li Y., Wang L., Zhang M., Melamed J., Liu X., Reiter R., Wei J., Peng Y., Zou X., Pellicer A., Garabedian M.J., Ferrari A., Lee P.
    Cancer Res 69:3332-3338(2009) [PubMed] [Europe PMC] [Abstract]
  • "SLUG: a new target of lymphoid enhancer factor-1 in human osteoblasts."
    Lambertini E., Franceschetti T., Torreggiani E., Penolazzi L., Pastore A., Pelucchi S., Gambari R., Piva R.
    BMC Mol Biol 11:13-13(2010) [PubMed] [Europe PMC] [Abstract]
  • "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements."
    Chen Q.Y., Zhang T., Pincus S.H., Wu S., Ricks D., Liu D., Sun Z., Maclaren N., Lan M.S.
    J Immunol 184:5047-5054(2010) [PubMed] [Europe PMC] [Abstract]
  • "Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro."
    Sheridan P.L., Sheline C.T., Cannon K., Voz M.L., Pazin M.J., Kadonaga J.T., Jones K.A.
    Genes Dev 9:2090-2104(1995) [PubMed] [Europe PMC] [Abstract]
  • "A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus."
    Brannon M., Gomperts M., Sumoy L., Moon R.T., Kimelman D.
    Genes Dev. 11:2359-2370(1997) [PubMed] [Europe PMC] [Abstract]
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCLInferred from direct assayⁱ
  • "Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis."
    Skokowa J., Klimiankou M., Klimenkova O., Lan D., Gupta K., Hussein K., Carrizosa E., Kusnetsova I., Li Z., Sustmann C., Ganser A., Zeidler C., Kreipe H.H., Burkhardt J., Grosschedl R., Welte K.
    Nat Med 18:1550-1559(2012) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: BHF-UCLInferred from direct assayⁱ
  • "Differential mechanisms of LEF/TCF family-dependent transcriptional activation by beta-catenin and plakoglobin."
    Zhurinsky J., Shtutman M., Ben-Ze'ev A.
    Mol Cell Biol 20:4238-4252(2000) [PubMed] [Europe PMC] [Abstract]
• sequence-specific double-stranded DNA binding Source: ARUK-UCLInferred from direct assayⁱ
  • "Impact of cytosine methylation on DNA binding specificities of human transcription factors."
    Yin Y., Morgunova E., Jolma A., Kaasinen E., Sahu B., Khund-Sayeed S., Das P.K., Kivioja T., Dave K., Zhong F., Nitta K.R., Taipale M., Popov A., Ginno P.A., Domcke S., Yan J., Schubeler D., Vinson C., Taipale J.
    Science 356:0-0(2017) [PubMed] [Europe PMC] [Abstract]
• transcription regulator inhibitor activity Source: GO_CentralInferred from direct assayⁱ
  • "Nkx3-1 and LEF-1 function as transcriptional inhibitors of estrogen receptor activity."
    Holmes K.A., Song J.S., Liu X.S., Brown M., Carroll J.S.
    Cancer Res 68:7380-7385(2008) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• anatomical structure regression Source: Ensembl
• apoptotic process involved in blood vessel morphogenesis Source: Ensembl
• B cell proliferation Source: Ensembl
• BMP signaling pathway Source: Ensembl
• branching involved in blood vessel morphogenesis Source: Ensembl
• canonical Wnt signaling pathway Source: BHF-UCLInferred from direct assayⁱ
  • "Protein phosphatase 2Calpha dephosphorylates axin and activates LEF-1-dependent transcription."
    Strovel E.T., Wu D., Sussman D.J.
    J Biol Chem 275:2399-2403(2000) [PubMed] [Europe PMC] [Abstract]
  • "Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures."
    Humtsoe J.O., Liu M., Malik A.B., Wary K.K.
    Mol. Cell. Biol. 30:1593-1606(2010) [PubMed] [Europe PMC] [Abstract]
• cell chemotaxis Source: UniProtKBInferred from direct assayⁱ
  • "WNT/TCF signaling through LEF1 and HOXB9 mediates lung adenocarcinoma metastasis."
    Nguyen D.X., Chiang A.C., Zhang X.H., Kim J.Y., Kris M.G., Ladanyi M., Gerald W.L., Massague J.
    Cell 138:51-62(2009) [PubMed] [Europe PMC] [Abstract]
• cellular response to cytokine stimulus Source: BHF-UCLInferred from mutant phenotypeⁱ
  • "Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis."
    Skokowa J., Klimiankou M., Klimenkova O., Lan D., Gupta K., Hussein K., Carrizosa E., Kusnetsova I., Li Z., Sustmann C., Ganser A., Zeidler C., Kreipe H.H., Burkhardt J., Grosschedl R., Welte K.
    Nat Med 18:1550-1559(2012) [PubMed] [Europe PMC] [Abstract]
• cellular response to interleukin-4 Source: UniProtKBInferred from direct assayⁱ
  • "LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element."
    Hebenstreit D., Giaisi M., Treiber M.K., Zhang X.B., Mi H.F., Horejs-Hoeck J., Andersen K.G., Krammer P.H., Duschl A., Li-Weber M.
    J Biol Chem 283:22490-22497(2008) [PubMed] [Europe PMC] [Abstract]
• chorio-allantoic fusion Source: Ensembl
• dentate gyrus development Source: Ensembl
• embryonic limb morphogenesis Source: Ensembl
• epithelial to mesenchymal transition Source: BHF-UCL
• face morphogenesis Source: Ensembl
• forebrain neuroblast division Source: Ensembl
• forebrain radial glial cell differentiation Source: Ensembl
• formation of radial glial scaffolds Source: Ensembl
• histone H3 acetylation Source: UniProtKBInferred from mutant phenotypeⁱ
  • "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements."
    Chen Q.Y., Zhang T., Pincus S.H., Wu S., Ricks D., Liu D., Sun Z., Maclaren N., Lan M.S.
    J Immunol 184:5047-5054(2010) [PubMed] [Europe PMC] [Abstract]
• histone H3-K56 acetylation Source: Ensembl
• histone H4 acetylation Source: UniProtKBInferred from mutant phenotypeⁱ
  • "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements."
    Chen Q.Y., Zhang T., Pincus S.H., Wu S., Ricks D., Liu D., Sun Z., Maclaren N., Lan M.S.
    J Immunol 184:5047-5054(2010) [PubMed] [Europe PMC] [Abstract]
• mammary gland development Source: Ensembl
• negative regulation of apoptotic process Source: UniProtKBInferred from mutant phenotypeⁱ
  • "Small molecule inhibitors of Wnt/beta-catenin/lef-1 signaling induces apoptosis in chronic lymphocytic leukemia cells in vitro and in vivo."
    Gandhirajan R.K., Staib P.A., Minke K., Gehrke I., Plickert G., Schlosser A., Schmitt E.K., Hallek M., Kreuzer K.A.
    Neoplasia 12:326-335(2010) [PubMed] [Europe PMC] [Abstract]
• negative regulation of apoptotic process in bone marrow cell Source: UniProtKBInferred from mutant phenotypeⁱ
  • "LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia."
    Skokowa J., Cario G., Uenalan M., Schambach A., Germeshausen M., Battmer K., Zeidler C., Lehmann U., Eder M., Baum C., Grosschedl R., Stanulla M., Scherr M., Welte K.
    Nat Med 12:1191-1197(2006) [PubMed] [Europe PMC] [Abstract]
• negative regulation of cell-cell adhesion Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• negative regulation of DNA binding Source: UniProtKBInferred from direct assayⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• negative regulation of interleukin-13 production Source: UniProtKBInferred from direct assayⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• negative regulation of interleukin-4 production Source: UniProtKBInferred from direct assayⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• negative regulation of interleukin-5 production Source: UniProtKBInferred from direct assayⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• negative regulation of striated muscle tissue development Source: Ensembl
• negative regulation of transcription, DNA-templated Source: UniProtKBInferred from direct assayⁱ
  • "Nkx3-1 and LEF-1 function as transcriptional inhibitors of estrogen receptor activity."
    Holmes K.A., Song J.S., Liu X.S., Brown M., Carroll J.S.
    Cancer Res 68:7380-7385(2008) [PubMed] [Europe PMC] [Abstract]
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• neutrophil differentiation Source: UniProtKBInferred from mutant phenotypeⁱ
  • "LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia."
    Skokowa J., Cario G., Uenalan M., Schambach A., Germeshausen M., Battmer K., Zeidler C., Lehmann U., Eder M., Baum C., Grosschedl R., Stanulla M., Scherr M., Welte K.
    Nat Med 12:1191-1197(2006) [PubMed] [Europe PMC] [Abstract]
• odontogenesis of dentin-containing tooth Source: Ensembl
• osteoblast differentiation Source: UniProtKB <p>Inferred from Expression Pattern</p> <p>Covers cases where the annotation is inferred from the timing or location of expression of a gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iep">GO evidence code guide</a></p> Inferred from expression patternⁱ
  • "SLUG: a new target of lymphoid enhancer factor-1 in human osteoblasts."
    Lambertini E., Franceschetti T., Torreggiani E., Penolazzi L., Pastore A., Pelucchi S., Gambari R., Piva R.
    BMC Mol Biol 11:13-13(2010) [PubMed] [Europe PMC] [Abstract]
• paraxial mesoderm formation Source: Ensembl
• positive regulation by host of viral transcription Source: UniProtKBInferred from direct assayⁱ
  • "Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro."
    Sheridan P.L., Sheline C.T., Cannon K., Voz M.L., Pazin M.J., Kadonaga J.T., Jones K.A.
    Genes Dev 9:2090-2104(1995) [PubMed] [Europe PMC] [Abstract]
• positive regulation of cell-cell adhesion Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• positive regulation of cell cycle process Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• positive regulation of cell migration Source: UniProtKBInferred from direct assayⁱ
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• positive regulation of cell population proliferation Source: UniProtKBInferred from direct assayⁱ
  • "LEF1 in androgen-independent prostate cancer: regulation of androgen receptor expression, prostate cancer growth, and invasion."
    Li Y., Wang L., Zhang M., Melamed J., Liu X., Reiter R., Wei J., Peng Y., Zou X., Pellicer A., Garabedian M.J., Ferrari A., Lee P.
    Cancer Res 69:3332-3338(2009) [PubMed] [Europe PMC] [Abstract]
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• positive regulation of cell proliferation in bone marrow Source: UniProtKBInferred from mutant phenotypeⁱ
  • "LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia."
    Skokowa J., Cario G., Uenalan M., Schambach A., Germeshausen M., Battmer K., Zeidler C., Lehmann U., Eder M., Baum C., Grosschedl R., Stanulla M., Scherr M., Welte K.
    Nat Med 12:1191-1197(2006) [PubMed] [Europe PMC] [Abstract]
• positive regulation of chondrocyte proliferation Source: Ensembl
• positive regulation of epithelial to mesenchymal transition Source: UniProtKBInferred from mutant phenotypeⁱ
  • "Type I collagen promotes epithelial-mesenchymal transition through ILK-dependent activation of NF-kappaB and LEF-1."
    Medici D., Nawshad A.
    Matrix Biol 29:161-165(2010) [PubMed] [Europe PMC] [Abstract]
• positive regulation of gamma-delta T cell differentiation Source: UniProtKB
• positive regulation of gene expression Source: AgBaseInferred from direct assayⁱ
  • "Differential use of functional domains by coiled-coil coactivator in its synergistic coactivator function with beta-catenin or GRIP1."
    Yang C.K., Kim J.H., Li H., Stallcup M.R.
    J. Biol. Chem. 281:3389-3397(2006) [PubMed] [Europe PMC] [Abstract]
• positive regulation of granulocyte differentiation Source: UniProtKBInferred from direct assayⁱ
  • "Neutrophil elastase is severely down-regulated in severe congenital neutropenia independent of ELA2 or HAX1 mutations but dependent on LEF-1."
    Skokowa J., Fobiwe J.P., Dan L., Thakur B.K., Welte K.
    Blood 114:3044-3051(2009) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription, DNA-templated Source: UniProtKBInferred from direct assayⁱ
  • "LEF-1 is crucial for neutrophil granulocytopoiesis and its expression is severely reduced in congenital neutropenia."
    Skokowa J., Cario G., Uenalan M., Schambach A., Germeshausen M., Battmer K., Zeidler C., Lehmann U., Eder M., Baum C., Grosschedl R., Stanulla M., Scherr M., Welte K.
    Nat Med 12:1191-1197(2006) [PubMed] [Europe PMC] [Abstract]
  • "Neutrophil elastase is severely down-regulated in severe congenital neutropenia independent of ELA2 or HAX1 mutations but dependent on LEF-1."
    Skokowa J., Fobiwe J.P., Dan L., Thakur B.K., Welte K.
    Blood 114:3044-3051(2009) [PubMed] [Europe PMC] [Abstract]
  • "SLUG: a new target of lymphoid enhancer factor-1 in human osteoblasts."
    Lambertini E., Franceschetti T., Torreggiani E., Penolazzi L., Pastore A., Pelucchi S., Gambari R., Piva R.
    BMC Mol Biol 11:13-13(2010) [PubMed] [Europe PMC] [Abstract]
  • "Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro."
    Sheridan P.L., Sheline C.T., Cannon K., Voz M.L., Pazin M.J., Kadonaga J.T., Jones K.A.
    Genes Dev 9:2090-2104(1995) [PubMed] [Europe PMC] [Abstract]
  • Ref.3

    "Lef-1 isoforms regulate different target genes and reduce cellular adhesion."
    Jesse S., Koenig A., Ellenrieder V., Menke A.
    Int. J. Cancer 126:1109-1120(2010) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY.
• positive regulation of transcription by RNA polymerase II Source: BHF-UCLInferred from direct assayⁱ
  • "Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro."
    Tutter A.V., Fryer C.J., Jones K.A.
    Genes Dev. 15:3342-3354(2001) [PubMed] [Europe PMC] [Abstract]
  • "Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis."
    Skokowa J., Klimiankou M., Klimenkova O., Lan D., Gupta K., Hussein K., Carrizosa E., Kusnetsova I., Li Z., Sustmann C., Ganser A., Zeidler C., Kreipe H.H., Burkhardt J., Grosschedl R., Welte K.
    Nat Med 18:1550-1559(2012) [PubMed] [Europe PMC] [Abstract]
• positive regulation of Wnt signaling pathway Source: Ensembl
• regulation of transcription by RNA polymerase II Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• secondary palate development Source: BHF-UCL
• sensory perception of taste Source: Ensembl
• somitogenesis Source: Ensembl
• sprouting angiogenesis Source: Ensembl
• T cell receptor V(D)J recombination Source: Ensembl
• T-helper 1 cell differentiation Source: UniProtKB
• tongue development Source: Ensembl
• trachea gland development Source: Ensembl

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

Organism-specific databases

Miscellaneous databases

Chemistry databases

Genetic variation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationⁱ

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• armadillo repeat domain binding Source: BHF-UCLInferred from physical interactionⁱ
  • "ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules."
    Daniels D.L., Weis W.I.
    Mol. Cell 10:573-584(2002) [PubMed] [Europe PMC] [Abstract]
• beta-catenin binding Source: BHF-UCLInferred from direct assayⁱ
  • Ref.10

    "Two members of the Tcf family implicated in Wnt/b-catenin signaling during embryogenesis in the mouse."
    Korinek V., Barker N., Willert K., Molenaar M., Roose J., Wagenaar G., Markman M., Lamers W., Destree O., Clevers H.
    Mol. Cell. Biol. 18:1248-1256(1998) [PubMed] [Europe PMC] [Abstract]

    Cited for: INTERACTION WITH CTNNB1.
• C2H2 zinc finger domain binding Source: UniProtKBInferred from physical interactionⁱ
  • "Lymphoid enhancer factor interacts with GATA-3 and controls its function in T helper type 2 cells."
    Hossain M.B., Hosokawa H., Hasegawa A., Watarai H., Taniguchi M., Yamashita M., Nakayama T.
    Immunology 125:377-386(2008) [PubMed] [Europe PMC] [Abstract]
• estrogen receptor binding Source: UniProtKBInferred from direct assayⁱ
  • "Nkx3-1 and LEF-1 function as transcriptional inhibitors of estrogen receptor activity."
    Holmes K.A., Song J.S., Liu X.S., Brown M., Carroll J.S.
    Cancer Res 68:7380-7385(2008) [PubMed] [Europe PMC] [Abstract]
• gamma-catenin binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Plakoglobin (gamma-catenin) has TCF/LEF family-dependent transcriptional activity in beta-catenin-deficient cell line."
    Maeda O., Usami N., Kondo M., Takahashi M., Goto H., Shimokata K., Kusugami K., Sekido Y.
    Oncogene 23:964-972(2004) [PubMed] [Europe PMC] [Abstract]
• histone binding Source: UniProtKBInferred from physical interactionⁱ
  • "Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements."
    Chen Q.Y., Zhang T., Pincus S.H., Wu S., Ricks D., Liu D., Sun Z., Maclaren N., Lan M.S.
    J Immunol 184:5047-5054(2010) [PubMed] [Europe PMC] [Abstract]
• histone deacetylase binding Source: ParkinsonsUK-UCLInferred from physical interactionⁱ
  • "Dapper1 is a nucleocytoplasmic shuttling protein that negatively modulates Wnt signaling in the nucleus."
    Gao X., Wen J., Zhang L., Li X., Ning Y., Meng A., Chen Y.G.
    J. Biol. Chem. 283:35679-35688(2008) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

Compositional bias

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (7+)ⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

This entry has 7 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

        10         20         30         40         50
MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL 
        60         70         80         90        100
ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG 
       110        120        130        140        150
LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA 
       160        170        180        190        200
VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS 
       210        220        230        240        250
PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS RFSHHMIPGP 
       260        270        280        290        300
PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVKPQHEQRK EQEPKRPHIK 
       310        320        330        340        350
KPLNAFMLYM KEMRANVVAE CTLKESAAIN QILGRRWHAL SREEQAKYYE 
       360        370        380        390 
LARKERQLHM QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI  

        10         20         30         40         50
MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL 
        60         70         80         90        100
ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG 
       110        120        130        140        150
LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA 
       160        170        180        190        200
VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS 
       210        220        230        240        250
PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS RFSHHMIPGP 
       260        270        280        290        300
PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVCSAFLLPH PFLIPSTPSP 
       310        320 
NHHHHHLLGS LSMNRERSRS QKDLTLRSL                        

        10         20         30         40         50
MMPNMNNDPY MSNGSLSPPI PRTSNKVPVV QPSHAVHPLT PLITYSDEHF 
        60         70         80         90        100
SPGSHPSHIP SDVNSKQGMS RHPPAPDIPT FYPLSPGGVG QITPPLGWQG 
       110        120        130        140        150
QPVYPITGGF RQPYPSSLSV DTSMSRFSHH MIPGPPGPHT TGIPHPAIVT 
       160        170        180        190        200
PQVKQEHPHT DSDLMHVKPQ HEQRKEQEPK RPHIKKPLNA FMLYMKEMRA 
       210        220        230        240        250
NVVAECTLKE SAAINQILGR RWHALSREEQ AKYYELARKE RQLHMQLYPG 
       260        270        280 
WSARDNYGKK KKRKREKLQE SASGTGPRMT AAYI                  

        10         20         30         40         50
MMPNMNNDPY MSNGSLSPPI PRTSNKVPVV QPSHAVHPLT PLITYSDEHF 
        60         70         80         90        100
SPGSHPSHIP SDVNSKQGMS RHPPAPDIPT FYPLSPGGVG QITPPLGWQG 
       110        120        130        140        150
QPVYPITGGF RQPYPSSLSV DTSMSRFSHH MIPGPPGPHT TGIPHPAIVT 
       160        170        180        190        200
PQVKQEHPHT DSDLMHVCSA FLLPHPFLIP STPSPNHHHH HLLGSLSMNR 
       210 
ERSRSQKDLT LRSL                                        

        10         20         30         40         50
MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL 
        60         70         80         90        100
ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG 
       110        120        130        140        150
LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA 
       160        170        180        190        200
VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS 
       210        220        230        240        250
PGGVGQITPP LGWFSHHMIP GPPGPHTTGI PHPAIVTPQV KQEHPHTDSD 
       260        270        280        290        300
LMHVKPQHEQ RKEQEPKRPH IKKPLNAFML YMKEMRANVV AECTLKESAA 
       310        320        330        340        350
INQILGRRWH ALSREEQAKY YELARKERQL HMQLYPGWSA RDNYGKKKKR 
       360        370 
KREKLQESAS GTGPRMTAAY I                                

        10         20         30         40         50
MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL 
        60         70         80         90        100
ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG 
       110        120        130        140        150
LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA 
       160        170        180        190        200
VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS 
       210        220        230        240        250
PGGVGQITPP LGWFSHHMIP GPPGPHTTGI PHPAIVTPQV KQEHPHTDSD 
       260        270        280        290        300
LMHVKPQHEQ RKEQEPKRPH IKKPLNAFML YMKEMRANVV AECTLKESAA 
       310        320        330        340        350
INQILGRRWH ALSREEQAKY YELARKERQL HMQLYPGWSA RDNYGKKKKR 
       360        370        380 
KREKLQESAS GGKRSSFPTC KAKAATPGPL LEMEAC                

        10         20         30         40         50
MAEVARQAQT SQEPYHDKAR EHPDDGKHPD GGLYNKGPSY SSYSGYIMMP 
        60         70         80         90        100
NMNNDPYMSN GSLSPPIPRT SNKVPVVQPS HAVHPLTPLI TYSDEHFSPG 
       110        120        130        140        150
SHPSHIPSDV NSKQGMSRHP PAPDIPTFYP LSPGGVGQIT PPLGWFSHHM 
       160        170        180        190        200
IPGPPGPHTT GIPHPAIVTP QVKQEHPHTD SDLMHVKPQH EQRKEQEPKR 
       210        220        230        240        250
PHIKKPLNAF MLYMKEMRAN VVAECTLKES AAINQILGRR WHALSREEQA 
       260        270        280        290        300
KYYELARKER QLHMQLYPGW SARDNYGKKK KRKREKLQES ASGTGPRMTA 

AYI                                                    

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityⁱ

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Genetic variation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Human chromosome 4
   Human chromosome 4: entries, gene names and cross-references to MIM
2. Human entries with genetic variants
   List of human entries with genetic variants
3. Human variants curated from literature reports
   Index of human variants curated from literature reports
4. MIM cross-references
   Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
5. SIMILARITY comments
   Index of protein domains and families