UniProtKB - Q9UJA5 (TRM6_HUMAN)
Protein
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Gene
TRMT6
Organism
Homo sapiens (Human)
Status
Functioni
Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508). Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29107537, PubMed:29072297).3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 349 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 377 | SubstrateCombined sources1 Publication | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA methylation Source: UniProtKB
- tRNA methylation Source: InterPro
- tRNA modification Source: Reactome
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BRENDAi | 2.1.1.220, 2681 |
PathwayCommonsi | Q9UJA5 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Alternative name(s): mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM61 Publication tRNA(m1A58)-methyltransferase subunit TRM6 Short name: tRNA(m1A58)MTase subunit TRM6 |
Gene namesi | Name:TRMT6 Synonyms:KIAA1153, TRM6 ORF Names:CGI-09 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20900, TRMT6 |
neXtProti | NX_Q9UJA5 |
VEuPathDBi | HostDB:ENSG00000089195.14 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- tRNA (m1A) methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 51605 |
OpenTargetsi | ENSG00000089195 |
PharmGKBi | PA162407063 |
Miscellaneous databases
Pharosi | Q9UJA5, Tdark |
Genetic variation databases
BioMutai | TRMT6 |
DMDMi | 74753354 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000233098 | 1 – 497 | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Add BLAST | 497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 107 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 298 | PhosphoserineCombined sources | 1 | |
Modified residuei | 305 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9UJA5 |
jPOSTi | Q9UJA5 |
MassIVEi | Q9UJA5 |
PaxDbi | Q9UJA5 |
PeptideAtlasi | Q9UJA5 |
PRIDEi | Q9UJA5 |
ProteomicsDBi | 5222 84613 [Q9UJA5-1] 84614 [Q9UJA5-2] 84615 [Q9UJA5-3] |
PTM databases
iPTMneti | Q9UJA5 |
PhosphoSitePlusi | Q9UJA5 |
Expressioni
Tissue specificityi
Expressed in brain, liver, testis and ovary.1 Publication
Gene expression databases
Bgeei | ENSG00000089195, Expressed in secondary oocyte and 218 other tissues |
Genevisiblei | Q9UJA5, HS |
Organism-specific databases
HPAi | ENSG00000089195, Low tissue specificity |
Interactioni
Subunit structurei
Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.
1 Publication1 PublicationBinary interactionsi
Hide detailsQ9UJA5
Protein-protein interaction databases
BioGRIDi | 119634, 58 interactors |
IntActi | Q9UJA5, 28 interactors |
MINTi | Q9UJA5 |
STRINGi | 9606.ENSP00000203001 |
Miscellaneous databases
RNActi | Q9UJA5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UJA5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 94 – 104 | Substrate bindingCombined sources1 PublicationAdd BLAST | 11 | |
Regioni | 145 – 154 | Substrate bindingCombined sources1 Publication | 10 | |
Regioni | 175 – 182 | Substrate bindingCombined sources1 Publication | 8 | |
Regioni | 415 – 423 | Substrate bindingCombined sources1 Publication | 9 | |
Regioni | 434 – 441 | Substrate bindingCombined sources1 Publication | 8 |
Sequence similaritiesi
Belongs to the TRM6/GCD10 family.Curated
Phylogenomic databases
eggNOGi | KOG1416, Eukaryota |
GeneTreei | ENSGT00390000008327 |
HOGENOMi | CLU_010916_0_1_1 |
InParanoidi | Q9UJA5 |
OMAi | ETCSGLV |
OrthoDBi | 543764at2759 |
PhylomeDBi | Q9UJA5 |
TreeFami | TF314835 |
Family and domain databases
InterProi | View protein in InterPro IPR017423, TRM6 |
PANTHERi | PTHR12945, PTHR12945, 1 hit |
Pfami | View protein in Pfam PF04189, Gcd10p, 1 hit |
PIRSFi | PIRSF038170, tRNA_m1A_mtfrase, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9UJA5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGSGEQPGP QPQHPGDHRI RDGDFVVLKR EDVFKAVQVQ RRKKVTFEKQ
60 70 80 90 100
WFYLDNVIGH SYGTAFEVTS GGSLQPKKKR EEPTAETKEA GTDNRNIVDD
110 120 130 140 150
GKSQKLTQDD IKALKDKGIK GEEIVQQLIE NSTTFRDKTE FAQDKYIKKK
160 170 180 190 200
KKKYEAIITV VKPSTRILSI MYYAREPGKI NHMRYDTLAQ MLTLGNIRAG
210 220 230 240 250
NKMIVMETCA GLVLGAMMER MGGFGSIIQL YPGGGPVRAA TACFGFPKSF
260 270 280 290 300
LSGLYEFPLN KVDSLLHGTF SAKMLSSEPK DSALVEESNG TLEEKQASEQ
310 320 330 340 350
ENEDSMAEAP ESNHPEDQET METISQDPEH KGPKERGSKK DYIQEKQRRQ
360 370 380 390 400
EEQRKRHLEA AALLSERNAD GLIVASRFHP TPLLLSLLDF VAPSRPFVVY
410 420 430 440 450
CQYKEPLLEC YTKLRERGGV INLRLSETWL RNYQVLPDRS HPKLLMSGGG
460 470 480 490
GYLLSGFTVA MDNLKADTSL KSNASTLESH ETEEPAAKKR KCPESDS
Sequence cautioni
The sequence BAA86467 differs from that shown. Reason: Frameshift.Curated
The sequence BAA86467 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053789 | 293 | E → K. Corresponds to variant dbSNP:rs6139876Ensembl. | 1 | |
Natural variantiVAR_053790 | 299 | E → G2 PublicationsCorresponds to variant dbSNP:rs451571Ensembl. | 1 | |
Natural variantiVAR_053791 | 333 | P → L. Corresponds to variant dbSNP:rs35203742Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054740 | 1 – 170 | Missing in isoform 4. 1 PublicationAdd BLAST | 170 | |
Alternative sequenceiVSP_031100 | 344 – 365 | QEKQR…AALLS → SGKNRGRQGRSSGKDFWGCR FA in isoform 3. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_031101 | 366 – 497 | Missing in isoform 3. 1 PublicationAdd BLAST | 132 | |
Alternative sequenceiVSP_018025 | 372 – 497 | LIVAS…PESDS → CVDGRRQETPQCMWALLQTD WHSLSPCL in isoform 2. 1 PublicationAdd BLAST | 126 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032979 mRNA Translation: BAA86467.1 Different initiation. AF132943 mRNA Translation: AAD27718.1 Frameshift. AK000613 mRNA No translation available. AK300812 mRNA Translation: BAG62468.1 AL035461 Genomic DNA No translation available. BC001262 mRNA Translation: AAH01262.1 |
CCDSi | CCDS13093.1 [Q9UJA5-1] CCDS63225.1 [Q9UJA5-4] |
RefSeqi | NP_001268396.1, NM_001281467.1 [Q9UJA5-4] NP_057023.2, NM_015939.4 [Q9UJA5-1] |
Genome annotation databases
Ensembli | ENST00000203001; ENSP00000203001; ENSG00000089195 [Q9UJA5-1] ENST00000453074; ENSP00000392070; ENSG00000089195 [Q9UJA5-4] |
GeneIDi | 51605 |
KEGGi | hsa:51605 |
UCSCi | uc002wmh.3, human [Q9UJA5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032979 mRNA Translation: BAA86467.1 Different initiation. AF132943 mRNA Translation: AAD27718.1 Frameshift. AK000613 mRNA No translation available. AK300812 mRNA Translation: BAG62468.1 AL035461 Genomic DNA No translation available. BC001262 mRNA Translation: AAH01262.1 |
CCDSi | CCDS13093.1 [Q9UJA5-1] CCDS63225.1 [Q9UJA5-4] |
RefSeqi | NP_001268396.1, NM_001281467.1 [Q9UJA5-4] NP_057023.2, NM_015939.4 [Q9UJA5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5CCB | X-ray | 2.00 | B | 1-497 | [»] | |
5CCX | X-ray | 2.10 | B | 1-497 | [»] | |
5CD1 | X-ray | 3.60 | B/E | 1-497 | [»] | |
SMRi | Q9UJA5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119634, 58 interactors |
IntActi | Q9UJA5, 28 interactors |
MINTi | Q9UJA5 |
STRINGi | 9606.ENSP00000203001 |
PTM databases
iPTMneti | Q9UJA5 |
PhosphoSitePlusi | Q9UJA5 |
Genetic variation databases
BioMutai | TRMT6 |
DMDMi | 74753354 |
Proteomic databases
EPDi | Q9UJA5 |
jPOSTi | Q9UJA5 |
MassIVEi | Q9UJA5 |
PaxDbi | Q9UJA5 |
PeptideAtlasi | Q9UJA5 |
PRIDEi | Q9UJA5 |
ProteomicsDBi | 5222 84613 [Q9UJA5-1] 84614 [Q9UJA5-2] 84615 [Q9UJA5-3] |
Protocols and materials databases
Antibodypediai | 23943, 78 antibodies |
DNASUi | 51605 |
Genome annotation databases
Ensembli | ENST00000203001; ENSP00000203001; ENSG00000089195 [Q9UJA5-1] ENST00000453074; ENSP00000392070; ENSG00000089195 [Q9UJA5-4] |
GeneIDi | 51605 |
KEGGi | hsa:51605 |
UCSCi | uc002wmh.3, human [Q9UJA5-1] |
Organism-specific databases
CTDi | 51605 |
DisGeNETi | 51605 |
GeneCardsi | TRMT6 |
HGNCi | HGNC:20900, TRMT6 |
HPAi | ENSG00000089195, Low tissue specificity |
neXtProti | NX_Q9UJA5 |
OpenTargetsi | ENSG00000089195 |
PharmGKBi | PA162407063 |
VEuPathDBi | HostDB:ENSG00000089195.14 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1416, Eukaryota |
GeneTreei | ENSGT00390000008327 |
HOGENOMi | CLU_010916_0_1_1 |
InParanoidi | Q9UJA5 |
OMAi | ETCSGLV |
OrthoDBi | 543764at2759 |
PhylomeDBi | Q9UJA5 |
TreeFami | TF314835 |
Enzyme and pathway databases
BRENDAi | 2.1.1.220, 2681 |
PathwayCommonsi | Q9UJA5 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 51605, 267 hits in 879 CRISPR screens |
ChiTaRSi | TRMT6, human |
GenomeRNAii | 51605 |
Pharosi | Q9UJA5, Tdark |
PROi | PR:Q9UJA5 |
RNActi | Q9UJA5, protein |
Gene expression databases
Bgeei | ENSG00000089195, Expressed in secondary oocyte and 218 other tissues |
Genevisiblei | Q9UJA5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR017423, TRM6 |
PANTHERi | PTHR12945, PTHR12945, 1 hit |
Pfami | View protein in Pfam PF04189, Gcd10p, 1 hit |
PIRSFi | PIRSF038170, tRNA_m1A_mtfrase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM6_HUMAN | |
Accessioni | Q9UJA5Primary (citable) accession number: Q9UJA5 Secondary accession number(s): B4DUV6 Q9Y2Z8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants