UniProtKB - Q9UIK4 (DAPK2_HUMAN)
Death-associated protein kinase 2
DAPK2
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 52 | ATPPROSITE-ProRule annotation2 Publications | 1 | |
Active sitei | 149 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 37 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- calmodulin binding Source: UniProtKB
- identical protein binding Source: IntAct
- protein serine/threonine kinase activity Source: GO_Central
GO - Biological processi
- anoikis Source: UniProtKB
- apoptotic process Source: UniProtKB
- intracellular signal transduction Source: UniProtKB
- positive regulation of apoptotic process Source: GO_Central
- positive regulation of eosinophil chemotaxis Source: UniProtKB
- positive regulation of neutrophil chemotaxis Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of autophagy Source: UniProtKB
- regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9UIK4 |
Reactomei | R-HSA-418889, Caspase activation via Dependence Receptors in the absence of ligand |
SignaLinki | Q9UIK4 |
SIGNORi | Q9UIK4 |
Names & Taxonomyi
Protein namesi | Recommended name: Death-associated protein kinase 2 (EC:2.7.11.12 Publications)Short name: DAP kinase 2 Alternative name(s): DAP-kinase-related protein 1 Short name: DRP-1 |
Gene namesi | Name:DAPK2 |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000035664.11 |
HGNCi | HGNC:2675, DAPK2 |
MIMi | 616567, gene |
neXtProti | NX_Q9UIK4 |
Subcellular locationi
Other locations
Golgi apparatus
- Golgi apparatus Source: HPA
Nucleus
- nucleus Source: GO_Central
Other locations
- autophagosome lumen Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesiclePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | K → A: Loss of activity, apoptotic function and of autophosphorylation. 3 Publications | 1 | |
Mutagenesisi | 299 – 330 | Missing : Loss of ca(2+)-calmodulin binding, increase in activity, loss of autophosphorylation. Add BLAST | 32 | |
Mutagenesisi | 299 | S → A: No effect on Ca(2+)-calmodulin independent phosphorylation or apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 318 | S → A: Loss of Ca(2+)-calmodulin independent phosphorylation, increase in apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 318 | S → D: Abolishes apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 320 | S → A: No effect on Ca(2+)-calmodulin independent phosphorylation or apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 323 | S → A: No effect on Ca(2+)-calmodulin independent phosphorylation or apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 329 | T → A: No effect on Ca(2+)-calmodulin independent phosphorylation or apoptotic activity. 1 Publication | 1 | |
Mutagenesisi | 367 | S → A: No effect on interaction with YWHAE. 1 Publication | 1 | |
Mutagenesisi | 368 | S → A: No effect on interaction with YWHAE. 1 Publication | 1 | |
Mutagenesisi | 369 | T → A: Interaction with YWHAE is reduced. 1 Publication | 1 | |
Mutagenesisi | 370 | S → A: Interaction with YWHAE is increased. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23604 |
OpenTargetsi | ENSG00000035664 |
PharmGKBi | PA27143 |
Miscellaneous databases
Pharosi | Q9UIK4, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3123 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9UIK4 |
Polymorphism and mutation databases
BioMutai | DAPK2 |
DMDMi | 38605084 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085912 | 1 – 370 | Death-associated protein kinase 2Add BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 299 | PhosphoserineCombined sources | 1 | |
Modified residuei | 318 | Phosphoserine; by autocatalysis1 Publication | 1 | |
Modified residuei | 349 | PhosphoserineCombined sources | 1 | |
Modified residuei | 369 | Phosphothreonine; by PKB/AKT11 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9UIK4 |
jPOSTi | Q9UIK4 |
MassIVEi | Q9UIK4 |
PaxDbi | Q9UIK4 |
PeptideAtlasi | Q9UIK4 |
PRIDEi | Q9UIK4 |
ProteomicsDBi | 19027 84537 [Q9UIK4-1] 84538 [Q9UIK4-2] |
PTM databases
iPTMneti | Q9UIK4 |
PhosphoSitePlusi | Q9UIK4 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000035664, Expressed in right lobe of thyroid gland and 221 other tissues |
ExpressionAtlasi | Q9UIK4, baseline and differential |
Genevisiblei | Q9UIK4, HS |
Organism-specific databases
HPAi | ENSG00000035664, Tissue enhanced (blood, skeletal muscle) |
Interactioni
Subunit structurei
Homodimer in its autoinhibited state. Active as monomer (By similarity). Isoform 2 but not isoform 1 can interact with ATF4.
Interacts with 14-3-3 proteins YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN; the interaction requires DAPK2 phosphorylation at Thr-369 and suppresses DAPK2 kinase activity and DAPK2-induced apoptosis (PubMed:26047703).
By similarity1 PublicationBinary interactionsi
Hide detailsQ9UIK4
With | #Exp. | IntAct |
---|---|---|
CCDC91 - isoform 2 [Q7Z6B0-2] | 3 | EBI-77154,EBI-12012082 |
itself | 4 | EBI-77154,EBI-77154 |
FAM9B [Q8IZU0] | 4 | EBI-77154,EBI-10175124 |
Isoform 2 [Q9UIK4-2]
With | #Exp. | IntAct |
---|---|---|
ATF4 [P18848] | 2 | EBI-9693115,EBI-492498 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 117137, 11 interactors |
IntActi | Q9UIK4, 9 interactors |
STRINGi | 9606.ENSP00000261891 |
Chemistry databases
BindingDBi | Q9UIK4 |
Miscellaneous databases
RNActi | Q9UIK4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UIK4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UIK4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 285 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 263 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Regioni | 277 – 344 | Calmodulin-bindingAdd BLAST | 68 | ||
Regioni | 292 – 301 | Autoinhibitory domainBy similarity | 10 | ||
Isoform 2 (identifier: Q9UIK4-2) | |||||
Regioni | 434 – 451 | Leucine-zipperCuratedAdd BLAST | 18 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000153424 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q9UIK4 |
OMAi | RWKLSYS |
OrthoDBi | 813445at2759 |
PhylomeDBi | Q9UIK4 |
TreeFami | TF314166 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFQASMRSPN MEPFKQQKVE DFYDIGEELG SGQFAIVKKC REKSTGLEYA
60 70 80 90 100
AKFIKKRQSR ASRRGVSREE IEREVSILRQ VLHHNVITLH DVYENRTDVV
110 120 130 140 150
LILELVSGGE LFDFLAQKES LSEEEATSFI KQILDGVNYL HTKKIAHFDL
160 170 180 190 200
KPENIMLLDK NIPIPHIKLI DFGLAHEIED GVEFKNIFGT PEFVAPEIVN
210 220 230 240 250
YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANIT AVSYDFDEEF
260 270 280 290 300
FSQTSELAKD FIRKLLVKET RKRLTIQEAL RHPWITPVDN QQAMVRRESV
310 320 330 340 350
VNLENFRKQY VRRRWKLSFS IVSLCNHLTR SLMKKVHLRP DEDLRNCESD
360 370
TEEDIARRKA LHPRRRSSTS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YKW8 | H0YKW8_HUMAN | Death-associated protein kinase 2 | DAPK2 | 80 | Annotation score: | ||
H0YLP5 | H0YLP5_HUMAN | Death-associated protein kinase 2 | DAPK2 | 88 | Annotation score: | ||
H0YMK1 | H0YMK1_HUMAN | Death-associated protein kinase 2 | DAPK2 | 91 | Annotation score: | ||
H0YL86 | H0YL86_HUMAN | Death-associated protein kinase 2 | DAPK2 | 48 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 241 | A → S in AAC35001 (PubMed:10629061).Curated | 1 | |
Sequence conflicti | 253 | Q → H in AAC35001 (PubMed:10629061).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040436 | 60 | R → W1 PublicationCorresponds to variant dbSNP:rs56047843Ensembl. | 1 | |
Natural variantiVAR_040437 | 271 | R → W1 PublicationCorresponds to variant dbSNP:rs34270163Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042057 | 287 – 370 | PVDNQ…RSSTS → SKGEGRAPEQRKTEPTQLKT KHLREYTLKCHSSMPPNNSY VNFERFACVVEDVARVDLGC RALVEAHDTIQDDVEALVSI FNEKEAWYREENESARHDLS QLRYEFRKVESLKKLLREDI QATGCSLGSMARKLDHLQAQ FEILRQELSADLQWIQELVG SFQLESGSSEGLGSTFYQDT SESLSELLSRSCTEEFLAGW KL in isoform 2. 1 PublicationAdd BLAST | 84 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB018001 mRNA Translation: BAA88063.1 AF052941 mRNA Translation: AAC35001.1 GU056176 mRNA Translation: ADD83109.1 AC015914 Genomic DNA No translation available. AC021541 Genomic DNA No translation available. BC114506 mRNA Translation: AAI14507.1 |
CCDSi | CCDS10188.1 [Q9UIK4-1] CCDS86463.1 [Q9UIK4-2] |
RefSeqi | NP_055141.2, NM_014326.3 [Q9UIK4-1] XP_011519718.1, XM_011521416.2 |
Genome annotation databases
Ensembli | ENST00000261891; ENSP00000261891; ENSG00000035664 [Q9UIK4-1] ENST00000457488; ENSP00000408277; ENSG00000035664 [Q9UIK4-1] ENST00000558069; ENSP00000453235; ENSG00000035664 [Q9UIK4-2] ENST00000612884; ENSP00000484390; ENSG00000035664 [Q9UIK4-2] |
GeneIDi | 23604 |
KEGGi | hsa:23604 |
UCSCi | uc002amr.4, human [Q9UIK4-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB018001 mRNA Translation: BAA88063.1 AF052941 mRNA Translation: AAC35001.1 GU056176 mRNA Translation: ADD83109.1 AC015914 Genomic DNA No translation available. AC021541 Genomic DNA No translation available. BC114506 mRNA Translation: AAI14507.1 |
CCDSi | CCDS10188.1 [Q9UIK4-1] CCDS86463.1 [Q9UIK4-2] |
RefSeqi | NP_055141.2, NM_014326.3 [Q9UIK4-1] XP_011519718.1, XM_011521416.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WMK | X-ray | 3.60 | A/B/C/D/E/F/G/H | 11-330 | [»] | |
1WRZ | X-ray | 2.00 | B | 312-330 | [»] | |
1Z9X | X-ray | 3.93 | A/B/C | 11-330 | [»] | |
1ZUZ | X-ray | 1.91 | B | 312-330 | [»] | |
1ZWS | X-ray | 2.90 | A/B/C/D/E/F/G/H | 11-297 | [»] | |
2A27 | X-ray | 3.00 | A/B/C/D/E/F/G/H | 11-330 | [»] | |
2A2A | X-ray | 1.47 | A/B/C/D | 11-330 | [»] | |
2CKE | X-ray | 2.80 | A/B/C/D | 11-330 | [»] | |
6PAW | X-ray | 2.95 | A/B/E/F | 12-330 | [»] | |
SMRi | Q9UIK4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117137, 11 interactors |
IntActi | Q9UIK4, 9 interactors |
STRINGi | 9606.ENSP00000261891 |
Chemistry databases
BindingDBi | Q9UIK4 |
ChEMBLi | CHEMBL3123 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9UIK4 |
PTM databases
iPTMneti | Q9UIK4 |
PhosphoSitePlusi | Q9UIK4 |
Polymorphism and mutation databases
BioMutai | DAPK2 |
DMDMi | 38605084 |
Proteomic databases
EPDi | Q9UIK4 |
jPOSTi | Q9UIK4 |
MassIVEi | Q9UIK4 |
PaxDbi | Q9UIK4 |
PeptideAtlasi | Q9UIK4 |
PRIDEi | Q9UIK4 |
ProteomicsDBi | 19027 84537 [Q9UIK4-1] 84538 [Q9UIK4-2] |
Protocols and materials databases
Antibodypediai | 13340, 598 antibodies |
DNASUi | 23604 |
Genome annotation databases
Ensembli | ENST00000261891; ENSP00000261891; ENSG00000035664 [Q9UIK4-1] ENST00000457488; ENSP00000408277; ENSG00000035664 [Q9UIK4-1] ENST00000558069; ENSP00000453235; ENSG00000035664 [Q9UIK4-2] ENST00000612884; ENSP00000484390; ENSG00000035664 [Q9UIK4-2] |
GeneIDi | 23604 |
KEGGi | hsa:23604 |
UCSCi | uc002amr.4, human [Q9UIK4-1] |
Organism-specific databases
CTDi | 23604 |
DisGeNETi | 23604 |
EuPathDBi | HostDB:ENSG00000035664.11 |
GeneCardsi | DAPK2 |
HGNCi | HGNC:2675, DAPK2 |
HPAi | ENSG00000035664, Tissue enhanced (blood, skeletal muscle) |
MIMi | 616567, gene |
neXtProti | NX_Q9UIK4 |
OpenTargetsi | ENSG00000035664 |
PharmGKBi | PA27143 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000153424 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q9UIK4 |
OMAi | RWKLSYS |
OrthoDBi | 813445at2759 |
PhylomeDBi | Q9UIK4 |
TreeFami | TF314166 |
Enzyme and pathway databases
PathwayCommonsi | Q9UIK4 |
Reactomei | R-HSA-418889, Caspase activation via Dependence Receptors in the absence of ligand |
SignaLinki | Q9UIK4 |
SIGNORi | Q9UIK4 |
Miscellaneous databases
BioGRID-ORCSi | 23604, 3 hits in 872 CRISPR screens |
ChiTaRSi | DAPK2, human |
EvolutionaryTracei | Q9UIK4 |
GeneWikii | DAPK2 |
GenomeRNAii | 23604 |
Pharosi | Q9UIK4, Tchem |
PROi | PR:Q9UIK4 |
RNActi | Q9UIK4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000035664, Expressed in right lobe of thyroid gland and 221 other tissues |
ExpressionAtlasi | Q9UIK4, baseline and differential |
Genevisiblei | Q9UIK4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAPK2_HUMAN | |
Accessioni | Q9UIK4Primary (citable) accession number: Q9UIK4 Secondary accession number(s): E9JGM7, O75892, Q24JS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2003 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human and mouse protein kinases
Human and mouse protein kinases: classification and index