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Protein

GTP:AMP phosphotransferase AK3, mitochondrial

Gene

AK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.UniRule annotation1 Publication

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38AMPUniRule annotation1
Binding sitei43AMPUniRule annotation1
Binding sitei98AMPUniRule annotation1
Binding sitei128GTPUniRule annotation1
Binding sitei161AMPUniRule annotation1
Binding sitei172AMPUniRule annotation1
Binding sitei201GTP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22GTPUniRule annotation6
Nucleotide bindingi64 – 66AMPUniRule annotation3
Nucleotide bindingi91 – 94AMPUniRule annotation4
Nucleotide bindingi137 – 138GTPUniRule annotation2

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • nucleoside triphosphate adenylate kinase activity Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase AK3, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Adenylate kinase 3 alpha-like 1UniRule annotation
Gene namesi
Name:AK3UniRule annotation
Synonyms:AK3L1, AK6, AKL3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000147853.16
HGNCiHGNC:17376 AK3
MIMi609290 gene
neXtProtiNX_Q9UIJ7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi50808
OpenTargetsiENSG00000147853
PharmGKBiPA164741184

Polymorphism and mutation databases

BioMutaiAK3
DMDMi23831297

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589221 – 227GTP:AMP phosphotransferase AK3, mitochondrialAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20N6-succinyllysineBy similarity1
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei57N6-succinyllysineBy similarity1
Modified residuei64N6-acetyllysine; alternateBy similarity1
Modified residuei64N6-succinyllysine; alternateBy similarity1
Modified residuei80N6-acetyllysine; alternateBy similarity1
Modified residuei80N6-succinyllysine; alternateBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei189N6-acetyllysine; alternateBy similarity1
Modified residuei189N6-succinyllysine; alternateBy similarity1
Modified residuei203N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UIJ7
PaxDbiQ9UIJ7
PeptideAtlasiQ9UIJ7
PRIDEiQ9UIJ7
ProteomicsDBi84535
84536 [Q9UIJ7-2]
TopDownProteomicsiQ9UIJ7-1 [Q9UIJ7-1]
Q9UIJ7-3 [Q9UIJ7-3]

2D gel databases

OGPiQ9UIJ7
REPRODUCTION-2DPAGEiIPI00465256
UCD-2DPAGEiQ9UIJ7

PTM databases

CarbonylDBiQ9UIJ7
iPTMnetiQ9UIJ7
PhosphoSitePlusiQ9UIJ7

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung.1 Publication

Gene expression databases

BgeeiENSG00000147853 Expressed in 205 organ(s), highest expression level in quadriceps femoris
CleanExiHS_AK3
HS_AK3L1
GenevisibleiQ9UIJ7 HS

Organism-specific databases

HPAiHPA063324

Interactioni

Subunit structurei

Monomer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
APPBP2Q926245EBI-3916527,EBI-743771

Protein-protein interaction databases

BioGridi119127, 8 interactors
IntActiQ9UIJ7, 10 interactors
STRINGi9606.ENSP00000371230

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UIJ7
SMRiQ9UIJ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UIJ7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 66NMPbindUniRule annotation1 PublicationAdd BLAST30
Regioni127 – 164LIDUniRule annotation1 PublicationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00550000074679
HOGENOMiHOG000238772
HOVERGENiHBG000458
InParanoidiQ9UIJ7
KOiK00944
OMAiVKNRWIH
OrthoDBiEOG091G06BH
PhylomeDBiQ9UIJ7
TreeFamiTF312916

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03169 Adenylate_kinase_AK3, 1 hit
InterProiView protein in InterPro
IPR006259 Adenyl_kin_sub
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR007862 Adenylate_kinase_lid-dom
IPR036193 ADK_active_lid_dom_sf
IPR028586 AK3/Ak4_mitochondrial
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PfamiView protein in Pfam
PF05191 ADK_lid, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
SSF57774 SSF57774, 1 hit
TIGRFAMsiTIGR01351 adk, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9UIJ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGASARLLRA VIMGAPGSGK GTVSSRITTH FELKHLSSGD LLRDNMLRGT
60 70 80 90 100
EIGVLAKAFI DQGKLIPDDV MTRLALHELK NLTQYSWLLD GFPRTLPQAE
110 120 130 140 150
ALDRAYQIDT VINLNVPFEV IKQRLTARWI HPASGRVYNI EFNPPKTVGI
160 170 180 190 200
DDLTGEPLIQ REDDKPETVI KRLKAYEDQT KPVLEYYQKK GVLETFSGTE
210 220
TNKIWPYVYA FLQTKVPQRS QKASVTP
Length:227
Mass (Da):25,565
Last modified:January 23, 2007 - v4
Checksum:i98A0EDF4FD9C9CEF
GO
Isoform 2 (identifier: Q9UIJ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:157
Mass (Da):18,192
Checksum:iF7D36803A8A241A4
GO
Isoform 3 (identifier: Q9UIJ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-90: Missing.

Note: No experimental confirmation available.
Show »
Length:187
Mass (Da):21,014
Checksum:i107B098298461090
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5A → G in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti38S → R in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti57K → Q in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti69 – 71DVM → YVT in BAA87913 (PubMed:11485571).Curated3
Sequence conflicti181K → E in BAG60470 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0430901 – 70Missing in isoform 2. 2 PublicationsAdd BLAST70
Alternative sequenceiVSP_04487651 – 90Missing in isoform 3. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021870 mRNA Translation: BAA87913.1
AF183419 mRNA Translation: AAG09688.1
AK001553 mRNA Translation: BAA91753.1
AK001951 mRNA Translation: BAA91996.1
AK027534 mRNA Translation: BAB55183.1
AK098205 mRNA Translation: BAG53592.1
AK298200 mRNA Translation: BAG60470.1
AL136231 Genomic DNA No translation available.
AL353151 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58779.1
CH471071 Genomic DNA Translation: EAW58780.1
CH471071 Genomic DNA Translation: EAW58781.1
BC013771 mRNA Translation: AAH13771.1
CCDSiCCDS56561.1 [Q9UIJ7-2]
CCDS56562.1 [Q9UIJ7-3]
CCDS6455.1 [Q9UIJ7-1]
RefSeqiNP_001186781.1, NM_001199852.1 [Q9UIJ7-3]
NP_001186782.1, NM_001199853.1 [Q9UIJ7-2]
NP_001186784.1, NM_001199855.1 [Q9UIJ7-2]
NP_001186785.1, NM_001199856.1 [Q9UIJ7-2]
NP_057366.2, NM_016282.3 [Q9UIJ7-1]
UniGeneiHs.732022

Genome annotation databases

EnsembliENST00000359883; ENSP00000352948; ENSG00000147853 [Q9UIJ7-2]
ENST00000381809; ENSP00000371230; ENSG00000147853 [Q9UIJ7-1]
ENST00000447596; ENSP00000413933; ENSG00000147853 [Q9UIJ7-3]
ENST00000611749; ENSP00000482308; ENSG00000147853 [Q9UIJ7-2]
GeneIDi50808
KEGGihsa:50808
UCSCiuc003ziq.4 human [Q9UIJ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021870 mRNA Translation: BAA87913.1
AF183419 mRNA Translation: AAG09688.1
AK001553 mRNA Translation: BAA91753.1
AK001951 mRNA Translation: BAA91996.1
AK027534 mRNA Translation: BAB55183.1
AK098205 mRNA Translation: BAG53592.1
AK298200 mRNA Translation: BAG60470.1
AL136231 Genomic DNA No translation available.
AL353151 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58779.1
CH471071 Genomic DNA Translation: EAW58780.1
CH471071 Genomic DNA Translation: EAW58781.1
BC013771 mRNA Translation: AAH13771.1
CCDSiCCDS56561.1 [Q9UIJ7-2]
CCDS56562.1 [Q9UIJ7-3]
CCDS6455.1 [Q9UIJ7-1]
RefSeqiNP_001186781.1, NM_001199852.1 [Q9UIJ7-3]
NP_001186782.1, NM_001199853.1 [Q9UIJ7-2]
NP_001186784.1, NM_001199855.1 [Q9UIJ7-2]
NP_001186785.1, NM_001199856.1 [Q9UIJ7-2]
NP_057366.2, NM_016282.3 [Q9UIJ7-1]
UniGeneiHs.732022

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZD8X-ray1.48A1-227[»]
ProteinModelPortaliQ9UIJ7
SMRiQ9UIJ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119127, 8 interactors
IntActiQ9UIJ7, 10 interactors
STRINGi9606.ENSP00000371230

PTM databases

CarbonylDBiQ9UIJ7
iPTMnetiQ9UIJ7
PhosphoSitePlusiQ9UIJ7

Polymorphism and mutation databases

BioMutaiAK3
DMDMi23831297

2D gel databases

OGPiQ9UIJ7
REPRODUCTION-2DPAGEiIPI00465256
UCD-2DPAGEiQ9UIJ7

Proteomic databases

EPDiQ9UIJ7
PaxDbiQ9UIJ7
PeptideAtlasiQ9UIJ7
PRIDEiQ9UIJ7
ProteomicsDBi84535
84536 [Q9UIJ7-2]
TopDownProteomicsiQ9UIJ7-1 [Q9UIJ7-1]
Q9UIJ7-3 [Q9UIJ7-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359883; ENSP00000352948; ENSG00000147853 [Q9UIJ7-2]
ENST00000381809; ENSP00000371230; ENSG00000147853 [Q9UIJ7-1]
ENST00000447596; ENSP00000413933; ENSG00000147853 [Q9UIJ7-3]
ENST00000611749; ENSP00000482308; ENSG00000147853 [Q9UIJ7-2]
GeneIDi50808
KEGGihsa:50808
UCSCiuc003ziq.4 human [Q9UIJ7-1]

Organism-specific databases

CTDi50808
DisGeNETi50808
EuPathDBiHostDB:ENSG00000147853.16
GeneCardsiAK3
HGNCiHGNC:17376 AK3
HPAiHPA063324
MIMi609290 gene
neXtProtiNX_Q9UIJ7
OpenTargetsiENSG00000147853
PharmGKBiPA164741184
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3078 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00550000074679
HOGENOMiHOG000238772
HOVERGENiHBG000458
InParanoidiQ9UIJ7
KOiK00944
OMAiVKNRWIH
OrthoDBiEOG091G06BH
PhylomeDBiQ9UIJ7
TreeFamiTF312916

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiAK3 human
EvolutionaryTraceiQ9UIJ7
GenomeRNAii50808
PROiPR:Q9UIJ7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147853 Expressed in 205 organ(s), highest expression level in quadriceps femoris
CleanExiHS_AK3
HS_AK3L1
GenevisibleiQ9UIJ7 HS

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03169 Adenylate_kinase_AK3, 1 hit
InterProiView protein in InterPro
IPR006259 Adenyl_kin_sub
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR007862 Adenylate_kinase_lid-dom
IPR036193 ADK_active_lid_dom_sf
IPR028586 AK3/Ak4_mitochondrial
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PfamiView protein in Pfam
PF05191 ADK_lid, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
SSF57774 SSF57774, 1 hit
TIGRFAMsiTIGR01351 adk, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKAD3_HUMAN
AccessioniPrimary (citable) accession number: Q9UIJ7
Secondary accession number(s): B4DP58
, D3DRI1, E7ET30, Q5VYW6, Q9H576, Q9HC01, Q9NPB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 176 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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