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UniProtKB - Q9UIC8 (LCMT1_HUMAN)
Protein
Leucine carboxyl methyltransferase 1
Gene
LCMT1
Organism
Homo sapiens (Human)
Status
Functioni
Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.
1 PublicationCatalytic activityi
- [phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.2332 Publications
Kineticsi
- KM=0.1 µM for PP2AD1 Publication
- KM=1.3 µM for AdoMet1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 73 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 98 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 122 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 198 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 |
GO - Molecular functioni
- protein C-terminal carboxyl O-methyltransferase activity Source: MGI
- protein C-terminal leucine carboxyl O-methyltransferase activity Source: GO_Central
- S-adenosylmethionine-dependent methyltransferase activity Source: ProtInc
GO - Biological processi
- cellular protein modification process Source: ProtInc
- C-terminal protein methylation Source: MGI
- G2/M transition of mitotic cell cycle Source: Reactome
- negative regulation of protein-containing complex assembly Source: MGI
- protein methylation Source: MGI
- regulation of apoptotic process Source: MGI
- regulation of glucose metabolic process Source: Ensembl
- regulation of mitotic cell cycle spindle assembly checkpoint Source: MGI
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.233, 2681 |
PathwayCommonsi | Q9UIC8 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition |
SABIO-RKi | Q9UIC8 |
SignaLinki | Q9UIC8 |
SIGNORi | Q9UIC8 |
Names & Taxonomyi
Protein namesi | Recommended name: Leucine carboxyl methyltransferase 1 (EC:2.1.1.2332 Publications)Alternative name(s): Protein-leucine O-methyltransferase [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1 |
Gene namesi | Name:LCMT1 Synonyms:LCMT ORF Names:CGI-68 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17557, LCMT1 |
MIMi | 610286, gene |
neXtProti | NX_Q9UIC8 |
VEuPathDBi | HostDB:ENSG00000205629 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 51451 |
OpenTargetsi | ENSG00000205629 |
PharmGKBi | PA134928443 |
Miscellaneous databases
Pharosi | Q9UIC8, Tbio |
Chemistry databases
DrugBanki | DB00149, Leucine |
Genetic variation databases
BioMutai | LCMT1 |
DMDMi | 12643251 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204422 | 1 – 334 | Leucine carboxyl methyltransferase 1Add BLAST | 334 |
Proteomic databases
EPDi | Q9UIC8 |
jPOSTi | Q9UIC8 |
MassIVEi | Q9UIC8 |
MaxQBi | Q9UIC8 |
PaxDbi | Q9UIC8 |
PeptideAtlasi | Q9UIC8 |
PRIDEi | Q9UIC8 |
ProteomicsDBi | 84491 [Q9UIC8-1] 84492 [Q9UIC8-2] 84493 [Q9UIC8-3] |
PTM databases
iPTMneti | Q9UIC8 |
MetOSitei | Q9UIC8 |
PhosphoSitePlusi | Q9UIC8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000205629, Expressed in testis and 238 other tissues |
ExpressionAtlasi | Q9UIC8, baseline and differential |
Genevisiblei | Q9UIC8, HS |
Organism-specific databases
HPAi | ENSG00000205629, Low tissue specificity |
Interactioni
Binary interactionsi
Q9UIC8
With | #Exp. | IntAct |
---|---|---|
FXR2 [P51116] | 2 | EBI-747632,EBI-740459 |
Protein-protein interaction databases
BioGRIDi | 119549, 21 interactors |
IntActi | Q9UIC8, 6 interactors |
MINTi | Q9UIC8 |
STRINGi | 9606.ENSP00000382021 |
Miscellaneous databases
RNActi | Q9UIC8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q9UIC8 |
SMRi | Q9UIC8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UIC8 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 171 – 172 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2918, Eukaryota |
GeneTreei | ENSGT00940000156372 |
HOGENOMi | CLU_031312_0_0_1 |
InParanoidi | Q9UIC8 |
OMAi | IIYEPIR |
OrthoDBi | 1094856at2759 |
PhylomeDBi | Q9UIC8 |
TreeFami | TF315087 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR016651, PPM1 IPR007213, Ppm1/Ppm2/Tcmp IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13600, PTHR13600, 1 hit |
Pfami | View protein in Pfam PF04072, LCM, 1 hit |
PIRSFi | PIRSF016305, LCM_mtfrase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9UIC8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATRQRESSI TSCCSTSSCD ADDEGVRGTC EDASLCKRFA VSIGYWHDPY
60 70 80 90 100
IQHFVRLSKE RKAPEINRGY FARVHGVSQL IKAFLRKTEC HCQIVNLGAG
110 120 130 140 150
MDTTFWRLKD EDLLPSKYFE VDFPMIVTRK LHSIKCKPPL SSPILELHSE
160 170 180 190 200
DTLQMDGHIL DSKRYAVIGA DLRDLSELEE KLKKCNMNTQ LPTLLIAECV
210 220 230 240 250
LVYMTPEQSA NLLKWAANSF ERAMFINYEQ VNMGDRFGQI MIENLRRRQC
260 270 280 290 300
DLAGVETCKS LESQKERLLS NGWETASAVD MMELYNRLPR AEVSRIESLE
310 320 330
FLDEMELLEQ LMRHYCLCWA TKGGNELGLK EITY
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L2Q8 | I3L2Q8_HUMAN | [Phosphatase 2A protein]-leucine-ca... | LCMT1 | 119 | Annotation score: | ||
I3L2E3 | I3L2E3_HUMAN | [Phosphatase 2A protein]-leucine-ca... | LCMT1 | 93 | Annotation score: | ||
I3L4A2 | I3L4A2_HUMAN | [Phosphatase 2A protein]-leucine-ca... | LCMT1 | 158 | Annotation score: | ||
I3L537 | I3L537_HUMAN | Leucine carboxyl methyltransferase ... | LCMT1 | 138 | Annotation score: | ||
H3BUP7 | H3BUP7_HUMAN | Leucine carboxyl methyltransferase ... | LCMT1 | 111 | Annotation score: | ||
H7BYF0 | H7BYF0_HUMAN | Leucine carboxyl methyltransferase ... | LCMT1 | 101 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 49 | P → S in BAD97084 (Ref. 4) Curated | 1 | |
Sequence conflicti | 114 | L → P in BAB15270 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 248 – 249 | RQ → PS in AAD34063 (PubMed:10810093).Curated | 2 | |
Sequence conflicti | 251 | D → E in AAF18267 (PubMed:10600115).Curated | 1 | |
Sequence conflicti | 301 | F → L in BAB15270 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 306 – 307 | EL → DV in AAF18267 (PubMed:10600115).Curated | 2 | |
Sequence conflicti | 312 – 313 | MR → NA in AAF18267 (PubMed:10600115).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017428 | 1 – 37 | MATRQ…ASLCK → MLPCARELPSAAPDSTLLLT AQHWVANLFILATLIGWTVR SFFLGRMETCCSHLGLRSGR in isoform 2. 3 PublicationsAdd BLAST | 37 | |
Alternative sequenceiVSP_041416 | 136 – 190 | Missing in isoform 3. CuratedAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF037601 mRNA Translation: AAF18267.1 AF151826 mRNA Translation: AAD34063.1 AK025884 mRNA Translation: BAB15270.1 AK223364 mRNA Translation: BAD97084.1 AK291885 mRNA Translation: BAF84574.1 AK314409 mRNA Translation: BAG37031.1 AC008741 Genomic DNA No translation available. AC133552 Genomic DNA No translation available. BC001214 mRNA Translation: AAH01214.1 BC014217 mRNA Translation: AAH14217.1 AL137283 mRNA Translation: CAB70677.1 |
CCDSi | CCDS45445.1 [Q9UIC8-1] CCDS45446.1 [Q9UIC8-3] |
PIRi | T46352 |
RefSeqi | NP_001027563.1, NM_001032391.1 [Q9UIC8-3] NP_057393.2, NM_016309.2 [Q9UIC8-1] XP_011544166.1, XM_011545864.1 [Q9UIC8-2] |
Genome annotation databases
Ensembli | ENST00000380966.8; ENSP00000370353.4; ENSG00000205629.12 [Q9UIC8-3] ENST00000399069.8; ENSP00000382021.3; ENSG00000205629.12 |
GeneIDi | 51451 |
KEGGi | hsa:51451 |
MANE-Selecti | ENST00000399069.8; ENSP00000382021.3; NM_016309.3; NP_057393.2 |
UCSCi | uc002dnx.2, human [Q9UIC8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF037601 mRNA Translation: AAF18267.1 AF151826 mRNA Translation: AAD34063.1 AK025884 mRNA Translation: BAB15270.1 AK223364 mRNA Translation: BAD97084.1 AK291885 mRNA Translation: BAF84574.1 AK314409 mRNA Translation: BAG37031.1 AC008741 Genomic DNA No translation available. AC133552 Genomic DNA No translation available. BC001214 mRNA Translation: AAH01214.1 BC014217 mRNA Translation: AAH14217.1 AL137283 mRNA Translation: CAB70677.1 |
CCDSi | CCDS45445.1 [Q9UIC8-1] CCDS45446.1 [Q9UIC8-3] |
PIRi | T46352 |
RefSeqi | NP_001027563.1, NM_001032391.1 [Q9UIC8-3] NP_057393.2, NM_016309.2 [Q9UIC8-1] XP_011544166.1, XM_011545864.1 [Q9UIC8-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3IEI | X-ray | 1.90 | A/B/C/D/E/F/G/H | 1-334 | [»] | |
3O7W | X-ray | 2.00 | A | 23-232 | [»] | |
A | 259-334 | [»] | ||||
3P71 | X-ray | 2.70 | T | 1-334 | [»] | |
AlphaFoldDBi | Q9UIC8 | |||||
SMRi | Q9UIC8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119549, 21 interactors |
IntActi | Q9UIC8, 6 interactors |
MINTi | Q9UIC8 |
STRINGi | 9606.ENSP00000382021 |
Chemistry databases
DrugBanki | DB00149, Leucine |
PTM databases
iPTMneti | Q9UIC8 |
MetOSitei | Q9UIC8 |
PhosphoSitePlusi | Q9UIC8 |
Genetic variation databases
BioMutai | LCMT1 |
DMDMi | 12643251 |
Proteomic databases
EPDi | Q9UIC8 |
jPOSTi | Q9UIC8 |
MassIVEi | Q9UIC8 |
MaxQBi | Q9UIC8 |
PaxDbi | Q9UIC8 |
PeptideAtlasi | Q9UIC8 |
PRIDEi | Q9UIC8 |
ProteomicsDBi | 84491 [Q9UIC8-1] 84492 [Q9UIC8-2] 84493 [Q9UIC8-3] |
Protocols and materials databases
Antibodypediai | 26192, 239 antibodies from 20 providers |
DNASUi | 51451 |
Genome annotation databases
Ensembli | ENST00000380966.8; ENSP00000370353.4; ENSG00000205629.12 [Q9UIC8-3] ENST00000399069.8; ENSP00000382021.3; ENSG00000205629.12 |
GeneIDi | 51451 |
KEGGi | hsa:51451 |
MANE-Selecti | ENST00000399069.8; ENSP00000382021.3; NM_016309.3; NP_057393.2 |
UCSCi | uc002dnx.2, human [Q9UIC8-1] |
Organism-specific databases
CTDi | 51451 |
DisGeNETi | 51451 |
GeneCardsi | LCMT1 |
HGNCi | HGNC:17557, LCMT1 |
HPAi | ENSG00000205629, Low tissue specificity |
MIMi | 610286, gene |
neXtProti | NX_Q9UIC8 |
OpenTargetsi | ENSG00000205629 |
PharmGKBi | PA134928443 |
VEuPathDBi | HostDB:ENSG00000205629 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2918, Eukaryota |
GeneTreei | ENSGT00940000156372 |
HOGENOMi | CLU_031312_0_0_1 |
InParanoidi | Q9UIC8 |
OMAi | IIYEPIR |
OrthoDBi | 1094856at2759 |
PhylomeDBi | Q9UIC8 |
TreeFami | TF315087 |
Enzyme and pathway databases
BRENDAi | 2.1.1.233, 2681 |
PathwayCommonsi | Q9UIC8 |
Reactomei | R-HSA-69273, Cyclin A/B1/B2 associated events during G2/M transition |
SABIO-RKi | Q9UIC8 |
SignaLinki | Q9UIC8 |
SIGNORi | Q9UIC8 |
Miscellaneous databases
BioGRID-ORCSi | 51451, 250 hits in 1090 CRISPR screens |
ChiTaRSi | LCMT1, human |
EvolutionaryTracei | Q9UIC8 |
GeneWikii | Leucine_carboxyl_methyltransferase_1 |
GenomeRNAii | 51451 |
Pharosi | Q9UIC8, Tbio |
PROi | PR:Q9UIC8 |
RNActi | Q9UIC8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000205629, Expressed in testis and 238 other tissues |
ExpressionAtlasi | Q9UIC8, baseline and differential |
Genevisiblei | Q9UIC8, HS |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR016651, PPM1 IPR007213, Ppm1/Ppm2/Tcmp IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13600, PTHR13600, 1 hit |
Pfami | View protein in Pfam PF04072, LCM, 1 hit |
PIRSFi | PIRSF016305, LCM_mtfrase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LCMT1_HUMAN | |
Accessioni | Q9UIC8Primary (citable) accession number: Q9UIC8 Secondary accession number(s): A6NL89 Q9Y378 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families