UniProtKB - Q9UI38 (TSP50_HUMAN)
Protein
Probable threonine protease PRSS50
Gene
PRSS50
Organism
Homo sapiens (Human)
Status
Functioni
May be involved in proteolysis through its threonine endopeptidase activity.1 Publication
Miscellaneous
DNA hypomethylation is accompanied by the expression of the gene in the testis.
Caution
Although related to peptidase S1 family, lacks the conserved active Ser residue in position 310 which is replaced by a Thr.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 153 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 206 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 310 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
- threonine-type endopeptidase activity Source: UniProtKB
GO - Biological processi
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Threonine protease |
Enzyme and pathway databases
PathwayCommonsi | Q9UI38 |
Protein family/group databases
MEROPSi | S01.993 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable threonine protease PRSS50 (EC:3.4.25.-1 Publication)Alternative name(s): Cancer/testis antigen 20 Serine protease 50 Testis-specific protease-like protein 50 |
Gene namesi | Name:PRSS50 Synonyms:CT20, TSP50 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17910, PRSS50 |
MIMi | 607950, gene |
neXtProti | NX_Q9UI38 |
VEuPathDBi | HostDB:ENSG00000206549.12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Note: May also localize to cytoplasmic membranes.
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 310 | T → A: Loss of catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 29122 |
OpenTargetsi | ENSG00000206549 |
PharmGKBi | PA165698443 |
Miscellaneous databases
Pharosi | Q9UI38, Tbio |
Genetic variation databases
BioMutai | PRSS50 |
DMDMi | 37089988 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 39 | Sequence analysisAdd BLAST | 39 | |
ChainiPRO_0000028492 | 40 – 385 | Probable threonine protease PRSS50Add BLAST | 346 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 138 ↔ 154 | PROSITE-ProRule annotation | ||
Disulfide bondi | 240 ↔ 316 | PROSITE-ProRule annotation | ||
Disulfide bondi | 273 ↔ 296 | PROSITE-ProRule annotation | ||
Glycosylationi | 279 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 306 ↔ 334 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | Q9UI38 |
PaxDbi | Q9UI38 |
PeptideAtlasi | Q9UI38 |
PRIDEi | Q9UI38 |
ProteomicsDBi | 84468 |
PTM databases
GlyGeni | Q9UI38, 2 sites |
iPTMneti | Q9UI38 |
PhosphoSitePlusi | Q9UI38 |
Expressioni
Tissue specificityi
Testis specific. Differentially expressed in some breast cancer tissues.
Gene expression databases
Bgeei | ENSG00000206549, Expressed in right testis and 105 other tissues |
ExpressionAtlasi | Q9UI38, baseline and differential |
Genevisiblei | Q9UI38, HS |
Organism-specific databases
HPAi | ENSG00000206549, Tissue enhanced (testis) ENSG00000283706, Tissue enriched (testis) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 118887, 25 interactors |
IntActi | Q9UI38, 18 interactors |
MINTi | Q9UI38 |
STRINGi | 9606.ENSP00000418875 |
Miscellaneous databases
RNActi | Q9UI38, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 358 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 266 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162593 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | Q9UI38 |
OMAi | MWPQFRT |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9UI38 |
TreeFami | TF351676 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9UI38-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRWCQTVAR GQRPRTSAPS RAGALLLLLL LLRSAGCWGA GEAPGALSTA
60 70 80 90 100
DPADQSVQCV PKATCPSSRP RLLWQTPTTQ TLPSTTMETQ FPVSEGKVDP
110 120 130 140 150
YRSCGFSYEQ DPTLRDPEAV ARRWPWMVSV RANGTHICAG TIIASQWVLT
160 170 180 190 200
VAHCLIWRDV IYSVRVGSPW IDQMTQTASD VPVLQVIMHS RYRAQRFWSW
210 220 230 240 250
VGQANDIGLL KLKQELKYSN YVRPICLPGT DYVLKDHSRC TVTGWGLSKA
260 270 280 290 300
DGMWPQFRTI QEKEVIILNN KECDNFYHNF TKIPTLVQII KSQMMCAEDT
310 320 330 340 350
HREKFCYELT GEPLVCSMEG TWYLVGLVSW GAGCQKSEAP PIYLQVSSYQ
360 370 380
HWIWDCLNGQ ALALPAPSRT LLLALPLPLS LLAAL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051859 | 75 | Q → P. Corresponds to variant dbSNP:rs34788938Ensembl. | 1 | |
Natural variantiVAR_051860 | 98 | V → I. Corresponds to variant dbSNP:rs35866901EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100707 mRNA Translation: AAF22500.1 BC033016 mRNA Translation: AAH33016.1 BC037775 mRNA Translation: AAH37775.1 |
CCDSi | CCDS2745.1 |
RefSeqi | NP_037402.1, NM_013270.4 |
Genome annotation databases
Ensembli | ENST00000315170; ENSP00000326598; ENSG00000283706 |
GeneIDi | 29122 |
KEGGi | hsa:29122 |
UCSCi | uc003cqe.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF100707 mRNA Translation: AAF22500.1 BC033016 mRNA Translation: AAH33016.1 BC037775 mRNA Translation: AAH37775.1 |
CCDSi | CCDS2745.1 |
RefSeqi | NP_037402.1, NM_013270.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 118887, 25 interactors |
IntActi | Q9UI38, 18 interactors |
MINTi | Q9UI38 |
STRINGi | 9606.ENSP00000418875 |
Protein family/group databases
MEROPSi | S01.993 |
PTM databases
GlyGeni | Q9UI38, 2 sites |
iPTMneti | Q9UI38 |
PhosphoSitePlusi | Q9UI38 |
Genetic variation databases
BioMutai | PRSS50 |
DMDMi | 37089988 |
Proteomic databases
MassIVEi | Q9UI38 |
PaxDbi | Q9UI38 |
PeptideAtlasi | Q9UI38 |
PRIDEi | Q9UI38 |
ProteomicsDBi | 84468 |
Protocols and materials databases
Antibodypediai | 35079, 66 antibodies |
DNASUi | 29122 |
Genome annotation databases
Ensembli | ENST00000315170; ENSP00000326598; ENSG00000283706 |
GeneIDi | 29122 |
KEGGi | hsa:29122 |
UCSCi | uc003cqe.2, human |
Organism-specific databases
CTDi | 29122 |
DisGeNETi | 29122 |
GeneCardsi | ENSG00000206549 PRSS50 |
HGNCi | HGNC:17910, PRSS50 |
HPAi | ENSG00000206549, Tissue enhanced (testis) ENSG00000283706, Tissue enriched (testis) |
MIMi | 607950, gene |
neXtProti | NX_Q9UI38 |
OpenTargetsi | ENSG00000206549 |
PharmGKBi | PA165698443 |
VEuPathDBi | HostDB:ENSG00000206549.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162593 |
HOGENOMi | CLU_006842_0_4_1 |
InParanoidi | Q9UI38 |
OMAi | MWPQFRT |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9UI38 |
TreeFami | TF351676 |
Enzyme and pathway databases
PathwayCommonsi | Q9UI38 |
Miscellaneous databases
BioGRID-ORCSi | 29122, 37 hits in 870 CRISPR screens |
GenomeRNAii | 29122 |
Pharosi | Q9UI38, Tbio |
PROi | PR:Q9UI38 |
RNActi | Q9UI38, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000206549, Expressed in right testis and 105 other tissues |
ExpressionAtlasi | Q9UI38, baseline and differential |
Genevisiblei | Q9UI38, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSP50_HUMAN | |
Accessioni | Q9UI38Primary (citable) accession number: Q9UI38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families