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Protein

Enolase-phosphatase E1

Gene

ENOPH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation1 Publication

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1), Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumUniRule annotation1 Publication1
Metal bindingi18Magnesium; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei187Substrate1
Metal bindingi212MagnesiumUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.77 2681
ReactomeiR-HSA-1237112 Methionine salvage pathway
UniPathwayi
UPA00904;UER00876

UPA00904;UER00877

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
MASA homologUniRule annotation
Gene namesi
Name:ENOPH1UniRule annotation
Synonyms:MASAUniRule annotation
ORF Names:MSTP145
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000145293.14
HGNCiHGNC:24599 ENOPH1
neXtProtiNX_Q9UHY7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi58478
OpenTargetsiENSG00000145293
PharmGKBiPA162385052

Chemistry databases

DrugBankiDB07912 2-OXOHEPTYLPHOSPHONIC ACID

Polymorphism and mutation databases

BioMutaiENOPH1
DMDMi74735024

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002540071 – 261Enolase-phosphatase E1Add BLAST261

Proteomic databases

EPDiQ9UHY7
PaxDbiQ9UHY7
PeptideAtlasiQ9UHY7
PRIDEiQ9UHY7
ProteomicsDBi84440
84441 [Q9UHY7-2]

PTM databases

DEPODiQ9UHY7
iPTMnetiQ9UHY7
PhosphoSitePlusiQ9UHY7

Expressioni

Gene expression databases

BgeeiENSG00000145293 Expressed in 225 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_ENOPH1
ExpressionAtlasiQ9UHY7 baseline and differential
GenevisibleiQ9UHY7 HS

Organism-specific databases

HPAiCAB004985
HPA044607

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF41Q9H4P44EBI-726969,EBI-2130266

Protein-protein interaction databases

BioGridi121811, 27 interactors
IntActiQ9UHY7, 12 interactors
MINTiQ9UHY7
STRINGi9606.ENSP00000273920

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UHY7
SMRiQ9UHY7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UHY7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 154Substrate binding2

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2630 Eukaryota
COG4229 LUCA
GeneTreeiENSGT00440000039914
HOGENOMiHOG000237286
HOVERGENiHBG054539
InParanoidiQ9UHY7
KOiK09880
OMAiHMWRAAY
OrthoDBiEOG091G0IX4
PhylomeDBiQ9UHY7
TreeFamiTF105939

Family and domain databases

CDDicd01629 HAD_EP, 1 hit
Gene3Di3.40.50.1000, 1 hit
HAMAPiMF_01681 Salvage_MtnC, 1 hit
MF_03117 Salvage_MtnC_euk, 1 hit
InterProiView protein in InterPro
IPR023943 Enolase-ppase_E1
IPR027511 ENOPH1_eukaryotes
IPR036412 HAD-like_sf
IPR006439 HAD-SF_hydro_IA
IPR023214 HAD_sf
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01691 enolase-ppase, 1 hit
TIGR01549 HAD-SF-IA-v1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UHY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ
110 120 130 140 150
MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY
160 170 180 190 200
IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI
210 220 230 240 250
GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT
260
SFSELYLPSS T
Length:261
Mass (Da):28,933
Last modified:May 1, 2000 - v1
Checksum:i12B3F73463907E2C
GO
Isoform 2 (identifier: Q9UHY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Show »
Length:115
Mass (Da):12,552
Checksum:i43E2FD7570657AFC
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RA00D6RA00_HUMAN
Enolase-phosphatase E1
ENOPH1
173Annotation score:
A0A0C4DGY8A0A0C4DGY8_HUMAN
Enolase-phosphatase E1
ENOPH1
149Annotation score:

Sequence cautioni

The sequence AAQ13671 differs from that shown. Reason: Frameshift at position 235.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211601 – 146Missing in isoform 2. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113125 mRNA Translation: AAF14866.1
AK022656 mRNA Translation: BAB14160.1
CR457141 mRNA Translation: CAG33422.1
BC001317 mRNA Translation: AAH01317.1
BC065815 mRNA Translation: AAH65815.1
AF177286 mRNA Translation: AAQ13671.1 Sequence problems.
CCDSiCCDS3594.1 [Q9UHY7-1]
RefSeqiNP_001278946.1, NM_001292017.1
NP_067027.1, NM_021204.4 [Q9UHY7-1]
UniGeneiHs.18442

Genome annotation databases

EnsembliENST00000273920; ENSP00000273920; ENSG00000145293 [Q9UHY7-1]
ENST00000505846; ENSP00000427209; ENSG00000145293 [Q9UHY7-2]
GeneIDi58478
KEGGihsa:58478
UCSCiuc003hmv.4 human [Q9UHY7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113125 mRNA Translation: AAF14866.1
AK022656 mRNA Translation: BAB14160.1
CR457141 mRNA Translation: CAG33422.1
BC001317 mRNA Translation: AAH01317.1
BC065815 mRNA Translation: AAH65815.1
AF177286 mRNA Translation: AAQ13671.1 Sequence problems.
CCDSiCCDS3594.1 [Q9UHY7-1]
RefSeqiNP_001278946.1, NM_001292017.1
NP_067027.1, NM_021204.4 [Q9UHY7-1]
UniGeneiHs.18442

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YNSX-ray1.70A1-261[»]
1ZS9X-ray1.70A1-261[»]
ProteinModelPortaliQ9UHY7
SMRiQ9UHY7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121811, 27 interactors
IntActiQ9UHY7, 12 interactors
MINTiQ9UHY7
STRINGi9606.ENSP00000273920

Chemistry databases

DrugBankiDB07912 2-OXOHEPTYLPHOSPHONIC ACID

PTM databases

DEPODiQ9UHY7
iPTMnetiQ9UHY7
PhosphoSitePlusiQ9UHY7

Polymorphism and mutation databases

BioMutaiENOPH1
DMDMi74735024

Proteomic databases

EPDiQ9UHY7
PaxDbiQ9UHY7
PeptideAtlasiQ9UHY7
PRIDEiQ9UHY7
ProteomicsDBi84440
84441 [Q9UHY7-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273920; ENSP00000273920; ENSG00000145293 [Q9UHY7-1]
ENST00000505846; ENSP00000427209; ENSG00000145293 [Q9UHY7-2]
GeneIDi58478
KEGGihsa:58478
UCSCiuc003hmv.4 human [Q9UHY7-1]

Organism-specific databases

CTDi58478
DisGeNETi58478
EuPathDBiHostDB:ENSG00000145293.14
GeneCardsiENOPH1
HGNCiHGNC:24599 ENOPH1
HPAiCAB004985
HPA044607
neXtProtiNX_Q9UHY7
OpenTargetsiENSG00000145293
PharmGKBiPA162385052
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2630 Eukaryota
COG4229 LUCA
GeneTreeiENSGT00440000039914
HOGENOMiHOG000237286
HOVERGENiHBG054539
InParanoidiQ9UHY7
KOiK09880
OMAiHMWRAAY
OrthoDBiEOG091G0IX4
PhylomeDBiQ9UHY7
TreeFamiTF105939

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00876

UPA00904;UER00877

BRENDAi3.1.3.77 2681
ReactomeiR-HSA-1237112 Methionine salvage pathway

Miscellaneous databases

ChiTaRSiENOPH1 human
EvolutionaryTraceiQ9UHY7
GenomeRNAii58478
PROiPR:Q9UHY7

Gene expression databases

BgeeiENSG00000145293 Expressed in 225 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_ENOPH1
ExpressionAtlasiQ9UHY7 baseline and differential
GenevisibleiQ9UHY7 HS

Family and domain databases

CDDicd01629 HAD_EP, 1 hit
Gene3Di3.40.50.1000, 1 hit
HAMAPiMF_01681 Salvage_MtnC, 1 hit
MF_03117 Salvage_MtnC_euk, 1 hit
InterProiView protein in InterPro
IPR023943 Enolase-ppase_E1
IPR027511 ENOPH1_eukaryotes
IPR036412 HAD-like_sf
IPR006439 HAD-SF_hydro_IA
IPR023214 HAD_sf
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01691 enolase-ppase, 1 hit
TIGR01549 HAD-SF-IA-v1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENOPH_HUMAN
AccessioniPrimary (citable) accession number: Q9UHY7
Secondary accession number(s): Q7Z4C5, Q9BVC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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