ENOPH1

Functionⁱ

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation1 Publication

Catalytic activityⁱ

5-(methylthio)-2,3-dioxopentyl phosphate + H₂O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactorⁱ

Mg²⁺Note: Binds 1 Mg²⁺ ion per subunit.

Pathwayⁱ: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:

Methylthioribose-1-phosphate isomerase (MRI1), Methylthioribose-1-phosphate isomerase (mtnA)
Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
Enolase-phosphatase E1 (ENOPH1)
Enolase-phosphatase E1 (ENOPH1)
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
no protein annotated in this organism

This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	16	MagnesiumUniRule annotation1 Publication	1
Metal bindingⁱ	18	Magnesium; via carbonyl oxygenUniRule annotation1 Publication	1
Binding siteⁱ	187	Substrate	1
Metal bindingⁱ	212	MagnesiumUniRule annotation1 Publication	1

GO - Molecular functionⁱ

acireductone synthase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 15843022
magnesium ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

L-methionine salvage from methylthioadenosine
Source: UniProtKB
Inferred from direct assayⁱ
- 15843022

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Amino-acid biosynthesis, Methionine biosynthesis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAⁱ	3.1.3.77 2681
Reactomeⁱ	R-HSA-1237112 Methionine salvage pathway
UniPathwayⁱ	UPA00904;UER00876 UPA00904;UER00877

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation) Alternative name(s): 2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation MASA homologUniRule annotation
Gene namesⁱ	Name:ENOPH1UniRule annotation Synonyms:MASAUniRule annotation ORF Names:MSTP145
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000145293.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:24599 ENOPH1
neXtProtⁱ	NX_Q9UHY7

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	58478
Open Targets More...OpenTargetsⁱ	ENSG00000145293
PharmGKBⁱ	PA162385052

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB07912 2-OXOHEPTYLPHOSPHONIC ACID

DrugBankⁱ

DB07912 2-OXOHEPTYLPHOSPHONIC ACID

Polymorphism and mutation databases

BioMutaⁱ	ENOPH1
DMDMⁱ	74735024

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000254007	1 – 261	Enolase-phosphatase E1Add BLAST		261

Proteomic databases

EPDⁱ	Q9UHY7
PaxDbⁱ	Q9UHY7
PeptideAtlas More...PeptideAtlasⁱ	Q9UHY7
PRIDEⁱ	Q9UHY7
ProteomicsDBⁱ	84440 84441 [Q9UHY7-2]

PTM databases

DEPODⁱ	Q9UHY7
iPTMnetⁱ	Q9UHY7
PhosphoSitePlusⁱ	Q9UHY7

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000145293 Expressed in 225 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExⁱ	HS_ENOPH1
ExpressionAtlasⁱ	Q9UHY7 baseline and differential
Genevisibleⁱ	Q9UHY7 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB004985 HPA044607

HPAⁱ

CAB004985
HPA044607

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
RNF41	Q9H4P4	4	EBI-726969,EBI-2130266

With

Entry

#Exp.

IntAct

Notes

RNF41

Q9H4P4

4

EBI-726969,EBI-2130266

Protein-protein interaction databases

BioGridⁱ	121811, 27 interactors
IntActⁱ	Q9UHY7, 12 interactors
Molecular INTeraction database More...MINTⁱ	Q9UHY7
STRINGⁱ	9606.ENSP00000273920

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	12 – 15	Combined sources	4
Turnⁱ	19 – 21	Combined sources	3
Helixⁱ	24 – 29	Combined sources	6
Helixⁱ	31 – 46	Combined sources	16
Helixⁱ	50 – 65	Combined sources	16
Turnⁱ	66 – 68	Combined sources	3
Helixⁱ	83 – 103	Combined sources	21
Helixⁱ	108 – 123	Combined sources	16
Helixⁱ	135 – 144	Combined sources	10
Beta strandⁱ	148 – 152	Combined sources	5
Helixⁱ	157 – 165	Combined sources	9
Helixⁱ	173 – 175	Combined sources	3
Beta strandⁱ	177 – 180	Combined sources	4
Helixⁱ	182 – 184	Combined sources	3
Helixⁱ	190 – 200	Combined sources	11
Helixⁱ	204 – 206	Combined sources	3
Beta strandⁱ	207 – 212	Combined sources	6
Helixⁱ	214 – 222	Combined sources	9
Beta strandⁱ	226 – 230	Combined sources	5
Helixⁱ	240 – 245	Combined sources	6
Beta strandⁱ	248 – 251	Combined sources	4
Helixⁱ	252 – 254	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9UHY7
SMRⁱ	Q9UHY7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9UHY7

EvolutionaryTraceⁱ

Q9UHY7

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	153 – 154	Substrate binding		2

Sequence similaritiesⁱ

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	KOG2630 Eukaryota COG4229 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00440000039914
HOGENOMⁱ	HOG000237286
HOVERGENⁱ	HBG054539
InParanoidⁱ	Q9UHY7
KEGG Orthology (KO) More...KOⁱ	K09880
OMAⁱ	HMWRAAY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0IX4
PhylomeDBⁱ	Q9UHY7
TreeFamⁱ	TF105939

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01629 HAD_EP, 1 hit
Gene3Dⁱ	3.40.50.1000, 2 hits
HAMAPⁱ	MF_01681 Salvage_MtnC, 1 hit MF_03117 Salvage_MtnC_euk, 1 hit
InterProⁱ	View protein in InterPro IPR023943 Enolase-ppase_E1 IPR027511 ENOPH1_eukaryotes IPR036412 HAD-like_sf IPR006439 HAD-SF_hydro_IA IPR023214 HAD_sf
SUPFAMⁱ	SSF56784 SSF56784, 1 hit
TIGRFAMsⁱ	TIGR01691 enolase-ppase, 1 hit TIGR01549 HAD-SF-IA-v1, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.ⁱShow all

Isoform 1 (identifier: Q9UHY7-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE 
        60         70         80         90        100
ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ 
       110        120        130        140        150
MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY 
       160        170        180        190        200
IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI 
       210        220        230        240        250
GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT 
       260 
SFSELYLPSS T

Length:261

Mass (Da):28,933

Last modified:May 1, 2000 - v1

Checksum:ⁱ12B3F73463907E2C

GO

Isoform 2 (identifier: Q9UHY7-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-146: Missing.

« Hide

        10         20         30         40         50
MKVYIYSSGS VEAQKLLFGH STEGDILELV DGHFDTKIGH KVESESYRKI 
        60         70         80         90        100
ADSIGCSTNN ILFLTDVTRE ASAAEEADVH VAVVVRPGNA GLTDDEKTYY 
       110 
SLITSFSELY LPSST

Show »

Length:115

Mass (Da):12,552

Checksum:ⁱ43E2FD7570657AFC

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	D6RA00	D6RA00_HUMAN		Enolase-phosphatase E1 Enolase-phosphatase E1	ENOPH1	173	Annotation score:
	A0A0C4DGY8	A0A0C4DGY8_HUMAN		Enolase-phosphatase E1 Enolase-phosphatase E1	ENOPH1	149	Annotation score:

Sequence cautionⁱ

The sequence AAQ13671 differs from that shown. Reason: Frameshift at position 235.Curated

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_021160	1 – 146	Missing in isoform 2. 1 PublicationAdd BLAST		146

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF113125 mRNA Translation: AAF14866.1 AK022656 mRNA Translation: BAB14160.1 CR457141 mRNA Translation: CAG33422.1 BC001317 mRNA Translation: AAH01317.1 BC065815 mRNA Translation: AAH65815.1 AF177286 mRNA Translation: AAQ13671.1 Sequence problems.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS3594.1 [Q9UHY7-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001278946.1, NM_001292017.1 NP_067027.1, NM_021204.4 [Q9UHY7-1]
UniGeneⁱ	Hs.18442

Genome annotation databases

Ensemblⁱ	ENST00000273920; ENSP00000273920; ENSG00000145293 [Q9UHY7-1] ENST00000505846; ENSP00000427209; ENSG00000145293 [Q9UHY7-2]
GeneIDⁱ	58478
KEGGⁱ	hsa:58478
UCSC genome browser More...UCSCⁱ	uc003hmv.4 human [Q9UHY7-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9UHY7	Enolase-phosphatase E1		PANTR		261	UniRef100_Q9UHY7
Enolase-phosphatase E1		PONAB		261
Enolase-phosphatase E1		PANPA		261
Enolase-phosphatase E1		NOMLE		261
Enolase-phosphatase E1		GORGO		261
+6

Protein	Similar proteins		Species	Score	Length	Source
Q9UHY7	Enolase-phosphatase E1		BOVIN		261	UniRef90_Q9UHY7
Enolase-phosphatase E1		PANTR		261
Enolase-phosphatase E1		PONAB		261
Enolase-phosphatase E1		PANPA		261
Enolase-phosphatase E1		NOMLE		261
+84

Protein	Similar proteins		Species	Score	Length	Source
Q9UHY7	Enolase-phosphatase E1		BOVIN		261	UniRef50_Q9UHY7
Enolase-phosphatase E1		XENLA		235
Enolase-phosphatase E1		SALSA		261
Enolase-phosphatase E1		DANRE		261
Enolase-phosphatase E1		XENTR		259
+270

Entry informationⁱ

Entry nameⁱ	ENOPH_HUMAN
Accessionⁱ	Q9UHY7Primary (citable) accession number: Q9UHY7 Secondary accession number(s): Q7Z4C5, Q9BVC2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
	Last sequence update:	May 1, 2000
	Last modified:	September 12, 2018
	This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9UHY7 (ENOPH_HUMAN)

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Enolase-phosphatase E1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-methionine biosynthesis via salvage pathway

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: L-methionine biosynthesis via salvage pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ