UniProtKB - Q9UHY7 (ENOPH_HUMAN)
Protein
Enolase-phosphatase E1
Gene
ENOPH1
Organism
Homo sapiens (Human)
Status
Functioni
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation1 Publication
Catalytic activityi
- 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphateUniRule annotationEC:3.1.3.77UniRule annotation
Cofactori
Mg2+Note: Binds 1 Mg2+ ion per subunit.
: L-methionine biosynthesis via salvage pathway Pathwayi
This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- Methylthioribose-1-phosphate isomerase (MRI1)
- Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
- Enolase-phosphatase E1 (ENOPH1)
- Enolase-phosphatase E1 (ENOPH1)
- 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 16 | MagnesiumUniRule annotation1 Publication | 1 | |
Metal bindingi | 18 | Magnesium; via carbonyl oxygenUniRule annotation1 Publication | 1 | |
Binding sitei | 187 | Substrate | 1 | |
Metal bindingi | 212 | MagnesiumUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Source: UniProtKB-UniRule
- 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Source: UniProtKB-UniRule
- acireductone synthase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- L-methionine salvage from methylthioadenosine Source: UniProtKB
- L-methionine salvage from S-adenosylmethionine Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.77, 2681 |
PathwayCommonsi | Q9UHY7 |
Reactomei | R-HSA-1237112, Methionine salvage pathway |
UniPathwayi | UPA00904;UER00876 UPA00904;UER00877 |
Names & Taxonomyi
Protein namesi | Recommended name: Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)Alternative name(s): 2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation MASA homologUniRule annotation |
Gene namesi | ORF Names:MSTP145 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000145293.14 |
HGNCi | HGNC:24599, ENOPH1 |
neXtProti | NX_Q9UHY7 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 58478 |
OpenTargetsi | ENSG00000145293 |
PharmGKBi | PA162385052 |
Miscellaneous databases
Pharosi | Q9UHY7, Tbio |
Chemistry databases
DrugBanki | DB07912, 2-OXOHEPTYLPHOSPHONIC ACID DB09325, Sodium fluoride |
Polymorphism and mutation databases
BioMutai | ENOPH1 |
DMDMi | 74735024 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000254007 | 1 – 261 | Enolase-phosphatase E1Add BLAST | 261 |
Proteomic databases
EPDi | Q9UHY7 |
jPOSTi | Q9UHY7 |
MassIVEi | Q9UHY7 |
PaxDbi | Q9UHY7 |
PeptideAtlasi | Q9UHY7 |
PRIDEi | Q9UHY7 |
ProteomicsDBi | 84440 [Q9UHY7-1] 84441 [Q9UHY7-2] |
PTM databases
DEPODi | ENOPH1 |
iPTMneti | Q9UHY7 |
PhosphoSitePlusi | Q9UHY7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000145293, Expressed in C1 segment of cervical spinal cord and 236 other tissues |
ExpressionAtlasi | Q9UHY7, baseline and differential |
Genevisiblei | Q9UHY7, HS |
Organism-specific databases
HPAi | ENSG00000145293, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Monomer.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 121811, 28 interactors |
IntActi | Q9UHY7, 16 interactors |
MINTi | Q9UHY7 |
STRINGi | 9606.ENSP00000273920 |
Miscellaneous databases
RNActi | Q9UHY7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UHY7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UHY7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 154 | Substrate binding | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2630, Eukaryota |
GeneTreei | ENSGT00440000039914 |
HOGENOMi | CLU_023273_0_0_1 |
InParanoidi | Q9UHY7 |
OMAi | HFDTHVG |
OrthoDBi | 1475361at2759 |
PhylomeDBi | Q9UHY7 |
TreeFami | TF105939 |
Family and domain databases
CDDi | cd01629, HAD_EP, 1 hit |
Gene3Di | 3.40.50.1000, 1 hit |
HAMAPi | MF_01681, Salvage_MtnC, 1 hit MF_03117, Salvage_MtnC_euk, 1 hit |
InterProi | View protein in InterPro IPR023943, Enolase-ppase_E1 IPR027511, ENOPH1_eukaryotes IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR023214, HAD_sf |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01691, enolase-ppase, 1 hit TIGR01549, HAD-SF-IA-v1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9UHY7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ
110 120 130 140 150
MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY
160 170 180 190 200
IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI
210 220 230 240 250
GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT
260
SFSELYLPSS T
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RA00 | D6RA00_HUMAN | Enolase-phosphatase E1 | ENOPH1 | 173 | Annotation score: | ||
A0A0C4DGY8 | A0A0C4DGY8_HUMAN | Enolase-phosphatase E1 | ENOPH1 | 149 | Annotation score: |
Sequence cautioni
The sequence AAQ13671 differs from that shown. Reason: Frameshift.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021160 | 1 – 146 | Missing in isoform 2. 1 PublicationAdd BLAST | 146 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF113125 mRNA Translation: AAF14866.1 AK022656 mRNA Translation: BAB14160.1 CR457141 mRNA Translation: CAG33422.1 BC001317 mRNA Translation: AAH01317.1 BC065815 mRNA Translation: AAH65815.1 AF177286 mRNA Translation: AAQ13671.1 Sequence problems. |
CCDSi | CCDS3594.1 [Q9UHY7-1] |
RefSeqi | NP_001278946.1, NM_001292017.1 NP_067027.1, NM_021204.4 [Q9UHY7-1] |
Genome annotation databases
Ensembli | ENST00000273920; ENSP00000273920; ENSG00000145293 [Q9UHY7-1] ENST00000505846; ENSP00000427209; ENSG00000145293 [Q9UHY7-2] |
GeneIDi | 58478 |
KEGGi | hsa:58478 |
UCSCi | uc003hmv.4, human [Q9UHY7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF113125 mRNA Translation: AAF14866.1 AK022656 mRNA Translation: BAB14160.1 CR457141 mRNA Translation: CAG33422.1 BC001317 mRNA Translation: AAH01317.1 BC065815 mRNA Translation: AAH65815.1 AF177286 mRNA Translation: AAQ13671.1 Sequence problems. |
CCDSi | CCDS3594.1 [Q9UHY7-1] |
RefSeqi | NP_001278946.1, NM_001292017.1 NP_067027.1, NM_021204.4 [Q9UHY7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YNS | X-ray | 1.70 | A | 1-261 | [»] | |
1ZS9 | X-ray | 1.70 | A | 1-261 | [»] | |
SMRi | Q9UHY7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121811, 28 interactors |
IntActi | Q9UHY7, 16 interactors |
MINTi | Q9UHY7 |
STRINGi | 9606.ENSP00000273920 |
Chemistry databases
DrugBanki | DB07912, 2-OXOHEPTYLPHOSPHONIC ACID DB09325, Sodium fluoride |
PTM databases
DEPODi | ENOPH1 |
iPTMneti | Q9UHY7 |
PhosphoSitePlusi | Q9UHY7 |
Polymorphism and mutation databases
BioMutai | ENOPH1 |
DMDMi | 74735024 |
Proteomic databases
EPDi | Q9UHY7 |
jPOSTi | Q9UHY7 |
MassIVEi | Q9UHY7 |
PaxDbi | Q9UHY7 |
PeptideAtlasi | Q9UHY7 |
PRIDEi | Q9UHY7 |
ProteomicsDBi | 84440 [Q9UHY7-1] 84441 [Q9UHY7-2] |
Protocols and materials databases
Antibodypediai | 25049, 159 antibodies |
Genome annotation databases
Ensembli | ENST00000273920; ENSP00000273920; ENSG00000145293 [Q9UHY7-1] ENST00000505846; ENSP00000427209; ENSG00000145293 [Q9UHY7-2] |
GeneIDi | 58478 |
KEGGi | hsa:58478 |
UCSCi | uc003hmv.4, human [Q9UHY7-1] |
Organism-specific databases
CTDi | 58478 |
DisGeNETi | 58478 |
EuPathDBi | HostDB:ENSG00000145293.14 |
GeneCardsi | ENOPH1 |
HGNCi | HGNC:24599, ENOPH1 |
HPAi | ENSG00000145293, Tissue enhanced (brain) |
neXtProti | NX_Q9UHY7 |
OpenTargetsi | ENSG00000145293 |
PharmGKBi | PA162385052 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2630, Eukaryota |
GeneTreei | ENSGT00440000039914 |
HOGENOMi | CLU_023273_0_0_1 |
InParanoidi | Q9UHY7 |
OMAi | HFDTHVG |
OrthoDBi | 1475361at2759 |
PhylomeDBi | Q9UHY7 |
TreeFami | TF105939 |
Enzyme and pathway databases
UniPathwayi | UPA00904;UER00876 UPA00904;UER00877 |
BRENDAi | 3.1.3.77, 2681 |
PathwayCommonsi | Q9UHY7 |
Reactomei | R-HSA-1237112, Methionine salvage pathway |
Miscellaneous databases
BioGRID-ORCSi | 58478, 9 hits in 850 CRISPR screens |
ChiTaRSi | ENOPH1, human |
EvolutionaryTracei | Q9UHY7 |
GenomeRNAii | 58478 |
Pharosi | Q9UHY7, Tbio |
PROi | PR:Q9UHY7 |
RNActi | Q9UHY7, protein |
Gene expression databases
Bgeei | ENSG00000145293, Expressed in C1 segment of cervical spinal cord and 236 other tissues |
ExpressionAtlasi | Q9UHY7, baseline and differential |
Genevisiblei | Q9UHY7, HS |
Family and domain databases
CDDi | cd01629, HAD_EP, 1 hit |
Gene3Di | 3.40.50.1000, 1 hit |
HAMAPi | MF_01681, Salvage_MtnC, 1 hit MF_03117, Salvage_MtnC_euk, 1 hit |
InterProi | View protein in InterPro IPR023943, Enolase-ppase_E1 IPR027511, ENOPH1_eukaryotes IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR023214, HAD_sf |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01691, enolase-ppase, 1 hit TIGR01549, HAD-SF-IA-v1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENOPH_HUMAN | |
Accessioni | Q9UHY7Primary (citable) accession number: Q9UHY7 Secondary accession number(s): Q7Z4C5, Q9BVC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families