UniProtKB - Q9UHP3 (UBP25_HUMAN)

BLAST

>sp|Q9UHP3|UBP25_HUMAN Ubiquitin carboxyl-terminal hydrolase 25 OS=Homo sapiens OX=9606 GN=USP25 PE=1 SV=4
MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAK
TPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRET
GITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWF
SAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYV
DPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFY
GRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQE
HWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIK
RLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSG
SIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHI
TEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHE
GQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQ
FLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQ
KLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSA
IKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERC
HNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFL
ICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNE
FIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIK
SFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

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History
Entry version 175 (12 Sep 2018)
Sequence version 4 (10 Aug 2010)
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Protein

Ubiquitin carboxyl-terminal hydrolase 25

Gene

USP25

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.

The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

Catalytic activityⁱ

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	178	1 Publication	1
Active siteⁱ	599	By similarity	1
Active siteⁱ	607	By similarity	1

GO - Molecular functionⁱ

ATPase binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 22590560
peptidase activity
Source: ProtInc
Traceable author statementⁱ
- 10644437
thiol-dependent ubiquitin-specific protease activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18538659
thiol-dependent ubiquitinyl hydrolase activity Source: Reactome
ubiquitin binding
Source: ParkinsonsUK-UCL
Non-traceable author statementⁱ
- 22590560
ubiquitin protein ligase binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 22590560
ubiquitin-specific protease activity involved in negative regulation of ERAD pathway
Source: ParkinsonsUK-UCL
Non-traceable author statementⁱ
- 22590560

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular protein modification process
Source: ProtInc
Traceable author statementⁱ
- 10644437
negative regulation of ERAD pathway
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 22590560
protein deubiquitination
Source: ParkinsonsUK-UCL
Inferred from direct assayⁱ
- 22590560
protein K48-linked deubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18538659
protein K63-linked deubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18538659
proteolysis
Source: ProtInc
Traceable author statementⁱ
- 10644437
ubiquitin-dependent protein catabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Biological process	Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5689880 Ub-specific processing proteases

Reactomeⁱ

R-HSA-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C19.041

MEROPSⁱ

C19.041

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin carboxyl-terminal hydrolase 25 (EC:3.4.19.12) Alternative name(s): Deubiquitinating enzyme 25 USP on chromosome 21 Ubiquitin thioesterase 25 Ubiquitin-specific-processing protease 25
Gene namesⁱ	Name:USP25 Synonyms:USP21
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 21

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000155313.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12624 USP25
MIMⁱ	604736 gene
neXtProtⁱ	NX_Q9UHP3

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	91	V → A: No interaction with SUMO3; when associated with A-92. 1 Publication	1
Mutagenesisⁱ	92	I → A: No interaction with SUMO3; when associated with A-91. 1 Publication	1
Mutagenesisⁱ	99	K → R: Abolishes sumoylation. Decreased enzymatic activity. 2 Publications	1
Mutagenesisⁱ	178	C → S: Abrogates deubiquitinating activity. No effect on homo- or oligomerization. 1 Publication	1
Mutagenesisⁱ	740	Y → F: No effect on interaction with SYK. 1 Publication	1
Mutagenesisⁱ	878	T → I: No effect on interaction with SYK. 1 Publication	1
Mutagenesisⁱ	880	Y → F: No effect on interaction with SYK. 1 Publication	1
Mutagenesisⁱ	883	I → S: No effect on interaction with SYK. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	29761
Open Targets More...OpenTargetsⁱ	ENSG00000155313
PharmGKBⁱ	PA37249

Polymorphism and mutation databases

DMDMⁱ	302393833

DMDMⁱ

302393833

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000080653	1 – 1055	Ubiquitin carboxyl-terminal hydrolase 25Add BLAST		1055

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	85	PhosphoserineCombined sources	1
Cross-linkⁱ	99	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linkⁱ	99	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Modified residueⁱ	740	Phosphotyrosine1 Publication	1

Post-translational modificationⁱ

Acetylated.1 Publication

Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.1 Publication

Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.1 Publication

Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.2 Publications

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9UHP3
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9UHP3
PaxDbⁱ	Q9UHP3
PeptideAtlas More...PeptideAtlasⁱ	Q9UHP3
PRIDEⁱ	Q9UHP3
ProteomicsDBⁱ	84385 84386 [Q9UHP3-1] 84387 [Q9UHP3-3]

PTM databases

iPTMnetⁱ	Q9UHP3
PhosphoSitePlusⁱ	Q9UHP3

Expressionⁱ

Tissue specificityⁱ

Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.2 Publications

Inductionⁱ

The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to 100-fold towards completion of differentiation.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000155313 Expressed in 225 organ(s), highest expression level in sperm
CleanExⁱ	HS_USP21 HS_USP25
ExpressionAtlasⁱ	Q9UHP3 baseline and differential
Genevisibleⁱ	Q9UHP3 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA018297 HPA024142

HPAⁱ

HPA018297
HPA024142

Interactionⁱ

Subunit structureⁱ

Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
MAGEB4	O15481	3	EBI-2513462,EBI-751857
RAD23A	P54725	7	EBI-2513462,EBI-746453
SUMO2	P61956	5	EBI-2513462,EBI-473220
SYK	P43405	6	EBI-2513462,EBI-78302
ZNF426	Q9BUY5	3	EBI-2513462,EBI-743265

GO - Molecular functionⁱ

ATPase binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 22590560
ubiquitin binding
Source: ParkinsonsUK-UCL
Non-traceable author statementⁱ
- 22590560
ubiquitin protein ligase binding
Source: ParkinsonsUK-UCL
Inferred from physical interactionⁱ
- 22590560

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	118895, 53 interactors
IntActⁱ	Q9UHP3, 26 interactors
Molecular INTeraction database More...MINTⁱ	Q9UHP3
STRINGⁱ	9606.ENSP00000285679

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	12 – 27	Combined sources	16
Helixⁱ	33 – 43	Combined sources	11
Helixⁱ	47 – 55	Combined sources	9
Beta strandⁱ	61 – 64	Combined sources	4
Beta strandⁱ	69 – 71	Combined sources	3
Helixⁱ	100 – 116	Combined sources	17
Helixⁱ	125 – 136	Combined sources	12

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9UHP3
SMRⁱ	Q9UHP3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	14 – 57	UBA-likeAdd BLAST	44
Domainⁱ	97 – 116	UIM 1PROSITE-ProRule annotationAdd BLAST	20
Domainⁱ	123 – 140	UIM 2PROSITE-ProRule annotationAdd BLAST	18
Domainⁱ	169 – 657	USPAdd BLAST	489

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	77 – 102	SUMO interaction domain (SIM)Add BLAST		26

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	541 – 578	Sequence analysisAdd BLAST		38
Coiled coilⁱ	684 – 717	Sequence analysisAdd BLAST		34

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	89 – 95	Required for SUMO paralog-specific binding		7

Sequence similaritiesⁱ

Belongs to the peptidase C19 family.Curated

Keywords - Domainⁱ

Coiled coil, Repeat

Phylogenomic databases

eggNOGⁱ	KOG1863 Eukaryota COG5077 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000016082
HOGENOMⁱ	HOG000007956
HOVERGENⁱ	HBG056030
InParanoidⁱ	Q9UHP3
KEGG Orthology (KO) More...KOⁱ	K11849
OMAⁱ	WAYIFDH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G00QR
PhylomeDBⁱ	Q9UHP3
TreeFamⁱ	TF329035

Family and domain databases

InterProⁱ	View protein in InterPro IPR038765 Papain_like_cys_pep_sf IPR001394 Peptidase_C19_UCH IPR009060 UBA-like_sf IPR003903 UIM_dom IPR018200 USP_CS IPR028889 USP_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00443 UCH, 1 hit PF02809 UIM, 2 hits
SMARTⁱ	View protein in SMART SM00726 UIM, 1 hit
SUPFAMⁱ	SSF46934 SSF46934, 1 hit SSF54001 SSF54001, 2 hits
PROSITEⁱ	View protein in PROSITE PS50330 UIM, 1 hit PS00972 USP_1, 1 hit PS00973 USP_2, 1 hit PS50235 USP_3, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform USP25a (identifier: Q9UHP3-2) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA 
        60         70         80         90        100
VAFLTAKNAK TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD 
       110        120        130        140        150
DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPTE 
       160        170        180        190        200
VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY 
       210        220        230        240        250
KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV DPSRAVEILK 
       260        270        280        290        300
DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 
       310        320        330        340        350
GRFLAVGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH 
       360        370        380        390        400
SENSGKSGQE HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY 
       410        420        430        440        450
LDRYMHRNRE ITRIKREEIK RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD 
       460        470        480        490        500
VLQYALEFAS SKPVCTSPVD DIDASSPPSG SIPSQTLPST TEQQGALSSE 
       510        520        530        540        550
LPSTSPSSVA AISSRSVIHK PFTQSRIPPD LPMHPAPRHI TEEELSVLES 
       560        570        580        590        600
CLHRWRTEIE NDTRDLQESI SRIHRTIELM YSDKSMIQVP YRLHAVLVHE 
       610        620        630        640        650
GQANAGHYWA YIFDHRESRW MKYNDIAVTK SSWEELVRDS FGGYRNASAY 
       660        670        680        690        700
CLMYINDKAQ FLIQEEFNKE TGQPLVGIET LPPDLRDFVE EDNQRFEKEL 
       710        720        730        740        750
EEWDAQLAQK ALQEKLLASQ KLRESETSVT TAQAAGDPEY LEQPSRSDFS 
       760        770        780        790        800
KHLKEETIQI ITKASHEHED KSPETVLQSA IKLEYARLVK LAQEDTPPET 
       810        820        830        840        850
DYRLHHVVVY FIQNQAPKKI IEKTLLEQFG DRNLSFDERC HNIMKVAQAK 
       860        870        880        890        900
LEMIKPEEVN LEEYEEWHQD YRKFRETTMY LIIGLENFQR ESYIDSLLFL 
       910        920        930        940        950
ICAYQNNKEL LSKGLYRGHD EELISHYRRE CLLKLNEQAA ELFESGEDRE 
       960        970        980        990       1000
VNNGLIIMNE FIVPFLPLLL VDEMEEKDIL AVEDMRNRWC SYLGQEMEPH 
      1010       1020       1030       1040       1050
LQEKLTDFLP KLLDCSMEIK SFHEPPKLPS YSTHELCERF ARIMLSLSRT 

PADGR

Length:1,055

Mass (Da):122,218

Last modified:August 10, 2010 - v4

Checksum:ⁱ0B9CECDCAD619CF2

Isoform USP25b (identifier: Q9UHP3-1) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
779-779: S → SIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFK

« Hide

        10         20         30         40         50
MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA 
        60         70         80         90        100
VAFLTAKNAK TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD 
       110        120        130        140        150
DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPTE 
       160        170        180        190        200
VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY 
       210        220        230        240        250
KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV DPSRAVEILK 
       260        270        280        290        300
DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 
       310        320        330        340        350
GRFLAVGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH 
       360        370        380        390        400
SENSGKSGQE HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY 
       410        420        430        440        450
LDRYMHRNRE ITRIKREEIK RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD 
       460        470        480        490        500
VLQYALEFAS SKPVCTSPVD DIDASSPPSG SIPSQTLPST TEQQGALSSE 
       510        520        530        540        550
LPSTSPSSVA AISSRSVIHK PFTQSRIPPD LPMHPAPRHI TEEELSVLES 
       560        570        580        590        600
CLHRWRTEIE NDTRDLQESI SRIHRTIELM YSDKSMIQVP YRLHAVLVHE 
       610        620        630        640        650
GQANAGHYWA YIFDHRESRW MKYNDIAVTK SSWEELVRDS FGGYRNASAY 
       660        670        680        690        700
CLMYINDKAQ FLIQEEFNKE TGQPLVGIET LPPDLRDFVE EDNQRFEKEL 
       710        720        730        740        750
EEWDAQLAQK ALQEKLLASQ KLRESETSVT TAQAAGDPEY LEQPSRSDFS 
       760        770        780        790        800
KHLKEETIQI ITKASHEHED KSPETVLQSI MMTPNMQGII MAIGKSRSVY 
       810        820        830        840        850
DRCGPEAGFF KAIKLEYARL VKLAQEDTPP ETDYRLHHVV VYFIQNQAPK 
       860        870        880        890        900
KIIEKTLLEQ FGDRNLSFDE RCHNIMKVAQ AKLEMIKPEE VNLEEYEEWH 
       910        920        930        940        950
QDYRKFRETT MYLIIGLENF QRESYIDSLL FLICAYQNNK ELLSKGLYRG 
       960        970        980        990       1000
HDEELISHYR RECLLKLNEQ AAELFESGED REVNNGLIIM NEFIVPFLPL 
      1010       1020       1030       1040       1050
LLVDEMEEKD ILAVEDMRNR WCSYLGQEME PHLQEKLTDF LPKLLDCSME 
      1060       1070       1080 
IKSFHEPPKL PSYSTHELCE RFARIMLSLS RTPADGR

Show »

Length:1,087

Mass (Da):125,750

Checksum:ⁱ8F852D91651B2C6E

Isoform USP25m (identifier: Q9UHP3-3) [UniParc]FASTA Add to basket

Also known as: Muscle-specific isoform

The sequence of this isoform differs from the canonical sequence as follows:
779-779: S → SKPENTTSQPLSNQRVVEVAIPHVGKFMIESKEGGYDDEIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFK

« Hide

        10         20         30         40         50
MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA 
        60         70         80         90        100
VAFLTAKNAK TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD 
       110        120        130        140        150
DLQRAIALSL AESNRAFRET GITDEEQAIS RVLEASIAEN KACLKRTPTE 
       160        170        180        190        200
VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF SAVIQSLFNL LEFRRLVLNY 
       210        220        230        240        250
KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV DPSRAVEILK 
       260        270        280        290        300
DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 
       310        320        330        340        350
GRFLAVGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH 
       360        370        380        390        400
SENSGKSGQE HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY 
       410        420        430        440        450
LDRYMHRNRE ITRIKREEIK RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD 
       460        470        480        490        500
VLQYALEFAS SKPVCTSPVD DIDASSPPSG SIPSQTLPST TEQQGALSSE 
       510        520        530        540        550
LPSTSPSSVA AISSRSVIHK PFTQSRIPPD LPMHPAPRHI TEEELSVLES 
       560        570        580        590        600
CLHRWRTEIE NDTRDLQESI SRIHRTIELM YSDKSMIQVP YRLHAVLVHE 
       610        620        630        640        650
GQANAGHYWA YIFDHRESRW MKYNDIAVTK SSWEELVRDS FGGYRNASAY 
       660        670        680        690        700
CLMYINDKAQ FLIQEEFNKE TGQPLVGIET LPPDLRDFVE EDNQRFEKEL 
       710        720        730        740        750
EEWDAQLAQK ALQEKLLASQ KLRESETSVT TAQAAGDPEY LEQPSRSDFS 
       760        770        780        790        800
KHLKEETIQI ITKASHEHED KSPETVLQSK PENTTSQPLS NQRVVEVAIP 
       810        820        830        840        850
HVGKFMIESK EGGYDDEIMM TPNMQGIIMA IGKSRSVYDR CGPEAGFFKA 
       860        870        880        890        900
IKLEYARLVK LAQEDTPPET DYRLHHVVVY FIQNQAPKKI IEKTLLEQFG 
       910        920        930        940        950
DRNLSFDERC HNIMKVAQAK LEMIKPEEVN LEEYEEWHQD YRKFRETTMY 
       960        970        980        990       1000
LIIGLENFQR ESYIDSLLFL ICAYQNNKEL LSKGLYRGHD EELISHYRRE 
      1010       1020       1030       1040       1050
CLLKLNEQAA ELFESGEDRE VNNGLIIMNE FIVPFLPLLL VDEMEEKDIL 
      1060       1070       1080       1090       1100
AVEDMRNRWC SYLGQEMEPH LQEKLTDFLP KLLDCSMEIK SFHEPPKLPS 
      1110       1120 
YSTHELCERF ARIMLSLSRT PADGR

Show »

Length:1,125

Mass (Da):129,963

Checksum:ⁱ85236A294FFEFD60

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q96B65	Q96B65_HUMAN	USP25 protein USP25 protein (Ubiquitin carboxyl-terminal hydrolase 25)	USP25	450	Annotation score:
H7C253	H7C253_HUMAN	Ubiquitin carboxyl-terminal hydrola... Ubiquitin carboxyl-terminal hydrolase 25	USP25	204	Annotation score:
H7C1T8	H7C1T8_HUMAN	Ubiquitin carboxyl-terminal hydrola... Ubiquitin carboxyl-terminal hydrolase 25	USP25	229	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	544	E → K in AAF32263 (PubMed:10644437).Curated		1
Sequence conflictⁱ	544	E → K in ACN76567 (Ref. 3) Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_039632	779	S → SIMMTPNMQGIIMAIGKSRS VYDRCGPEAGFFK in isoform USP25b. 1 Publication		1
Alternative sequenceⁱVSP_039631	779	S → SKPENTTSQPLSNQRVVEVA IPHVGKFMIESKEGGYDDEI MMTPNMQGIIMAIGKSRSVY DRCGPEAGFFK in isoform USP25m. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF170562 mRNA Translation: AAF32263.1 AF134213 mRNA Translation: AAF24998.1 FJ763652 mRNA Translation: ACN76567.1 CH471079 Genomic DNA Translation: EAX10041.1 BC075792 mRNA Translation: AAH75792.1 AK022574 mRNA Translation: BAB14107.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33515.1 [Q9UHP3-2] CCDS63336.1 [Q9UHP3-3] CCDS63337.1 [Q9UHP3-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001269970.1, NM_001283041.1 [Q9UHP3-3] NP_001269971.1, NM_001283042.1 [Q9UHP3-1] NP_037528.3, NM_013396.4 [Q9UHP3-2]
UniGeneⁱ	Hs.743994

Genome annotation databases

Ensemblⁱ	ENST00000285679; ENSP00000285679; ENSG00000155313 [Q9UHP3-2] ENST00000285681; ENSP00000285681; ENSG00000155313 [Q9UHP3-1] ENST00000400183; ENSP00000383044; ENSG00000155313 [Q9UHP3-3]
GeneIDⁱ	29761
KEGGⁱ	hsa:29761
UCSC genome browser More...UCSCⁱ	uc002yjy.3 human [Q9UHP3-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9UHP3	cDNA FLJ12385 fis, clone MAMMA1002619, weakly similar to PROBABLE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE K02C4.3		HUMAN		523	UniRef100_Q9UHP3
UPI0007DC7F9F		HUMAN		651
UPI0000EE49FC		HUMAN		834

Protein	Similar proteins		Species	Score	Length	Source
Q9UHP3	Ubiquitin carboxyl-terminal hydrolase 25	)	MOUSE		1055	UniRef90_Q9UHP3
cDNA FLJ12385 fis, clone MAMMA1002619, weakly similar to PROBABLE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE K02C4.3		HUMAN		523
Uncharacterized protein		PANPA		1095
ubiquitin carboxyl-terminal hydrolase 25 isoform X2		LIPVE		1072
Ubiquitin specific peptidase 25		OTOGA		1057
+100
Q9UHP3-1	Uncharacterized protein		AOTNA		1087	UniRef90_Q9UHP3-1
Uncharacterized protein		CALJA		1087
Uncharacterized protein		Cebus capucinus imitator		1087
Uncharacterized protein		PAPAN		1087
Ubiquitin specific peptidase 25		GORGO		1087
+61
Q9UHP3-3	Uncharacterized protein		PANPA		1174	UniRef90_Q9UHP3-3
Ubiquitin carboxyl-terminal hydrolase 25 (Fragment)		HUMAN		303
Ubiquitin specific peptidase 25		MUSPF		1127
USP25 isoform 4		PONAB		1125
Uncharacterized protein		CERAT		1125
+142

Protein	Similar proteins		Species	Score	Length	Source
Q9UHP3	Ubiquitin carboxyl-terminal hydrolase 25	)	MOUSE		1055	UniRef50_Q9UHP3
cDNA FLJ12385 fis, clone MAMMA1002619, weakly similar to PROBABLE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE K02C4.3		HUMAN		523
Uncharacterized protein		PANPA		1095
ubiquitin carboxyl-terminal hydrolase 25 isoform X2		LIPVE		1072
Ubiquitin carboxyl-terminal hydrolase 25		PTEAL		1057
+840

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF170562 mRNA Translation: AAF32263.1 AF134213 mRNA Translation: AAF24998.1 FJ763652 mRNA Translation: ACN76567.1 CH471079 Genomic DNA Translation: EAX10041.1 BC075792 mRNA Translation: AAH75792.1 AK022574 mRNA Translation: BAB14107.1
CCDSⁱ	CCDS33515.1 [Q9UHP3-2] CCDS63336.1 [Q9UHP3-3] CCDS63337.1 [Q9UHP3-1]
RefSeqⁱ	NP_001269970.1, NM_001283041.1 [Q9UHP3-3] NP_001269971.1, NM_001283042.1 [Q9UHP3-1] NP_037528.3, NM_013396.4 [Q9UHP3-2]
UniGeneⁱ	Hs.743994

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2MUX	NMR	-	A	1-146	[»]
	5GP7	X-ray	1.50	B	1046-1055	[»]
	5O71	X-ray	3.28	A	1-714	[»]
ProteinModelPortalⁱ	Q9UHP3
SMRⁱ	Q9UHP3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	118895, 53 interactors
IntActⁱ	Q9UHP3, 26 interactors
MINTⁱ	Q9UHP3
STRINGⁱ	9606.ENSP00000285679

Protein family/group databases

MEROPSⁱ	C19.041

MEROPSⁱ

C19.041

PTM databases

iPTMnetⁱ	Q9UHP3
PhosphoSitePlusⁱ	Q9UHP3

Polymorphism and mutation databases

DMDMⁱ	302393833

DMDMⁱ

302393833

Proteomic databases

EPDⁱ	Q9UHP3
MaxQBⁱ	Q9UHP3
PaxDbⁱ	Q9UHP3
PeptideAtlasⁱ	Q9UHP3
PRIDEⁱ	Q9UHP3
ProteomicsDBⁱ	84385 84386 [Q9UHP3-1] 84387 [Q9UHP3-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	29761
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000285679; ENSP00000285679; ENSG00000155313 [Q9UHP3-2] ENST00000285681; ENSP00000285681; ENSG00000155313 [Q9UHP3-1] ENST00000400183; ENSP00000383044; ENSG00000155313 [Q9UHP3-3]
GeneIDⁱ	29761
KEGGⁱ	hsa:29761
UCSCⁱ	uc002yjy.3 human [Q9UHP3-2]

Organism-specific databases

CTDⁱ	29761
DisGeNETⁱ	29761
EuPathDBⁱ	HostDB:ENSG00000155313.15
GeneCardsⁱ	USP25
HGNCⁱ	HGNC:12624 USP25
HPAⁱ	HPA018297 HPA024142
MIMⁱ	604736 gene
neXtProtⁱ	NX_Q9UHP3
OpenTargetsⁱ	ENSG00000155313
PharmGKBⁱ	PA37249
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1863 Eukaryota COG5077 LUCA
GeneTreeⁱ	ENSGT00390000016082
HOGENOMⁱ	HOG000007956
HOVERGENⁱ	HBG056030
InParanoidⁱ	Q9UHP3
KOⁱ	K11849
OMAⁱ	WAYIFDH
OrthoDBⁱ	EOG091G00QR
PhylomeDBⁱ	Q9UHP3
TreeFamⁱ	TF329035

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5689880 Ub-specific processing proteases

Reactomeⁱ

R-HSA-5689880 Ub-specific processing proteases

Miscellaneous databases

ChiTaRSⁱ	USP25 human
GenomeRNAiⁱ	29761
Protein Ontology More...PROⁱ	PR:Q9UHP3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000155313 Expressed in 225 organ(s), highest expression level in sperm
CleanExⁱ	HS_USP21 HS_USP25
ExpressionAtlasⁱ	Q9UHP3 baseline and differential
Genevisibleⁱ	Q9UHP3 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR038765 Papain_like_cys_pep_sf IPR001394 Peptidase_C19_UCH IPR009060 UBA-like_sf IPR003903 UIM_dom IPR018200 USP_CS IPR028889 USP_dom
Pfamⁱ	View protein in Pfam PF00443 UCH, 1 hit PF02809 UIM, 2 hits
SMARTⁱ	View protein in SMART SM00726 UIM, 1 hit
SUPFAMⁱ	SSF46934 SSF46934, 1 hit SSF54001 SSF54001, 2 hits
PROSITEⁱ	View protein in PROSITE PS50330 UIM, 1 hit PS00972 USP_1, 1 hit PS00973 USP_2, 1 hit PS50235 USP_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBP25_HUMAN
Accessionⁱ	Q9UHP3Primary (citable) accession number: Q9UHP3 Secondary accession number(s): C0LSZ0, Q6DHZ9, Q9H9W1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	August 10, 2010
	Last modified:	September 12, 2018
	This is version 175 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM
Peptidase families
Classification of peptidase families and list of entries
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniRef

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Supporting data

UniProtKB - Q9UHP3 (UBP25_HUMAN)

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Ubiquitin carboxyl-terminal hydrolase 25

USP25

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Nucleus

Other locations

Cytosol

Endoplasmic reticulum

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ