UniProtKB - Q9UHI6 (DDX20_HUMAN)
Probable ATP-dependent RNA helicase DDX20
DDX20
Functioni
Catalytic activityi
- EC:3.6.4.13
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 84 | ATP; via carbonyl oxygen | 1 | |
Binding sitei | 89 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 109 – 114 | ATP | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- repressing transcription factor binding Source: BHF-UCL
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
GO - Biological processi
- import into nucleus Source: Reactome
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of apoptotic process Source: MGI
- RNA processing Source: ProtInc
- spliceosomal snRNP assembly Source: UniProtKB
- spliceosomal tri-snRNP complex assembly Source: ProtInc
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9UHI6 |
Reactomei | R-HSA-191859, snRNP Assembly |
SIGNORi | Q9UHI6 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable ATP-dependent RNA helicase DDX20 (EC:3.6.4.13)Alternative name(s): Component of gems 3 DEAD box protein 20 DEAD box protein DP 103 Gemin-3 |
Gene namesi | Name:DDX20 Synonyms:DP103, GEMIN3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2743, DDX20 |
MIMi | 606168, gene |
neXtProti | NX_Q9UHI6 |
VEuPathDBi | HostDB:ENSG00000064703.11 |
Subcellular locationi
Cytoskeleton
- cytoskeleton Source: ProtInc
Cytosol
- cytosol Source: UniProtKB
Nucleus
- Gemini of coiled bodies Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: ProtInc
Other locations
- cytoplasm Source: ProtInc
- membrane Source: UniProtKB
- SMN complex Source: UniProtKB
- SMN-Sm protein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 11218 |
OpenTargetsi | ENSG00000064703 |
PharmGKBi | PA27209 |
Miscellaneous databases
Pharosi | Q9UHI6, Tbio |
Genetic variation databases
BioMutai | DDX20 |
DMDMi | 12643886 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055025 | 1 – 824 | Probable ATP-dependent RNA helicase DDX20Add BLAST | 824 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphoserineCombined sources | 1 | |
Modified residuei | 187 | PhosphoserineCombined sources | 1 | |
Modified residuei | 268 | PhosphoserineCombined sources | 1 | |
Modified residuei | 269 | PhosphoserineCombined sources | 1 | |
Modified residuei | 500 | PhosphoserineCombined sources | 1 | |
Modified residuei | 505 | PhosphoserineCombined sources | 1 | |
Modified residuei | 532 | PhosphoserineCombined sources | 1 | |
Modified residuei | 552 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 560 | PhosphoserineCombined sources | 1 | |
Modified residuei | 652 | PhosphoserineCombined sources | 1 | |
Modified residuei | 654 | PhosphoserineCombined sources | 1 | |
Modified residuei | 656 | PhosphoserineCombined sources | 1 | |
Modified residuei | 672 | PhosphoserineCombined sources | 1 | |
Modified residuei | 677 | PhosphoserineCombined sources | 1 | |
Modified residuei | 678 | PhosphoserineCombined sources | 1 | |
Modified residuei | 688 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 703 | PhosphoserineCombined sources | 1 | |
Modified residuei | 705 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 714 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9UHI6 |
jPOSTi | Q9UHI6 |
MassIVEi | Q9UHI6 |
PaxDbi | Q9UHI6 |
PeptideAtlasi | Q9UHI6 |
PRIDEi | Q9UHI6 |
ProteomicsDBi | 5385 84357 [Q9UHI6-1] |
PTM databases
iPTMneti | Q9UHI6 |
PhosphoSitePlusi | Q9UHI6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000064703, Expressed in testis and 205 other tissues |
ExpressionAtlasi | Q9UHI6, baseline and differential |
Genevisiblei | Q9UHI6, HS |
Organism-specific databases
HPAi | ENSG00000064703, Tissue enriched (testis) |
Interactioni
Subunit structurei
Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG.
Interacts directly with GEMIN5.
Interacts directly with SNUPN.
Interacts with PPP4R2.
Interacts with FOXL2.
Interacts with EBV EBNA2 and EBNA3C.
Interacts with NANOS1 and PUM2.
8 PublicationsBinary interactionsi
Hide detailsQ9UHI6
GO - Molecular functioni
- repressing transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 116387, 144 interactors |
ComplexPortali | CPX-6031, SMN complex |
CORUMi | Q9UHI6 |
DIPi | DIP-32606N |
IntActi | Q9UHI6, 66 interactors |
MINTi | Q9UHI6 |
STRINGi | 9606.ENSP00000358716 |
Miscellaneous databases
RNActi | Q9UHI6, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UHI6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UHI6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 264 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 172 | |
Domaini | 299 – 448 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 150 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 456 – 548 | SMN interactingAdd BLAST | 93 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 62 – 90 | Q motifAdd BLAST | 29 | |
Motifi | 211 – 214 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4284, Eukaryota |
GeneTreei | ENSGT00940000158400 |
HOGENOMi | CLU_1854485_0_0_1 |
InParanoidi | Q9UHI6 |
OMAi | AYHMNTV |
PhylomeDBi | Q9UHI6 |
TreeFami | TF352222 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAFEASGA LAAVATAMPA EHVAVQVPAP EPTPGPVRIL RTAQDLSSPR
60 70 80 90 100
TRTGDVLLAE PADFESLLLS RPVLEGLRAA GFERPSPVQL KAIPLGRCGL
110 120 130 140 150
DLIVQAKSGT GKTCVFSTIA LDSLVLENLS TQILILAPTR EIAVQIHSVI
160 170 180 190 200
TAIGIKMEGL ECHVFIGGTP LSQDKTRLKK CHIAVGSPGR IKQLIELDYL
210 220 230 240 250
NPGSIRLFIL DEADKLLEEG SFQEQINWIY SSLPASKQML AVSATYPEFL
260 270 280 290 300
ANALTKYMRD PTFVRLNSSD PSLIGLKQYY KVVNSYPLAH KVFEEKTQHL
310 320 330 340 350
QELFSRIPFN QALVFSNLHS RAQHLADILS SKGFPAECIS GNMNQNQRLD
360 370 380 390 400
AMAKLKHFHC RVLISTDLTS RGIDAEKVNL VVNLDVPLDW ETYMHRIGRA
410 420 430 440 450
GRFGTLGLTV TYCCRGEEEN MMMRIAQKCN INLLPLPDPI PSGLMEECVD
460 470 480 490 500
WDVEVKAAVH TYGIASVPNQ PLKKQIQKIE RTLQIQKAHG DHMASSRNNS
510 520 530 540 550
VSGLSVKSKN NTKQKLPVKS HSECGIIEKA TSPKELGCDR QSEEQMKNSV
560 570 580 590 600
QTPVENSTNS QHQVKEALPV SLPQIPCLSS FKIHQPYTLT FAELVEDYEH
610 620 630 640 650
YIKEGLEKPV EIIRHYTGPG DQTVNPQNGF VRNKVIEQRV PVLASSSQSG
660 670 680 690 700
DSESDSDSYS SRTSSQSKGN KSYLEGSSDN QLKDSESTPV DDRISLEQPP
710 720 730 740 750
NGSDTPNPEK YQESPGIQMK TRLKEGASQR AKQSRRNLPR RSSFRLQTEA
760 770 780 790 800
QEDDWYDCHR EIRLSFSDTY QDYEEYWRAY YRAWQEYYAA ASHSYYWNAQ
810 820
RHPSWMAAYH MNTIYLQEMM HSNQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PJ60 | E9PJ60_HUMAN | Probable ATP-dependent RNA helicase... | DDX20 | 432 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | F → V in AAD42744 (PubMed:10383418).Curated | 1 | |
Sequence conflicti | 279 | Y → C in BAA91727 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 639 | R → K in AAF14544 (PubMed:10601333).Curated | 1 | |
Sequence conflicti | 659 | Y → H in AAD42744 (PubMed:10383418).Curated | 1 | |
Sequence conflicti | 676 | G → S in AAF14544 (PubMed:10601333).Curated | 1 | |
Sequence conflicti | 703 | S → T in AAF14544 (PubMed:10601333).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057231 | 636 | I → T1 PublicationCorresponds to variant dbSNP:rs197412Ensembl. | 1 | |
Natural variantiVAR_057232 | 693 | R → S. Corresponds to variant dbSNP:rs197414Ensembl. | 1 | |
Natural variantiVAR_057233 | 762 | I → T. Corresponds to variant dbSNP:rs85276Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056505 | 133 – 138 | ILILAP → AELSNS in isoform 2. 1 Publication | 6 | |
Alternative sequenceiVSP_056506 | 139 – 824 | Missing in isoform 2. 1 PublicationAdd BLAST | 686 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171063 mRNA Translation: AAF14544.1 AF106019 mRNA Translation: AAD42744.1 AK001506 mRNA Translation: BAA91727.1 AK301697 mRNA Translation: BAG63169.1 AL049557 Genomic DNA No translation available. BC011556 mRNA Translation: AAH11556.1 AL133598 mRNA Translation: CAB63734.2 |
CCDSi | CCDS842.1 [Q9UHI6-1] |
PIRi | T43476 |
RefSeqi | NP_009135.4, NM_007204.4 [Q9UHI6-1] |
Genome annotation databases
Ensembli | ENST00000369702; ENSP00000358716; ENSG00000064703 [Q9UHI6-1] ENST00000533164; ENSP00000434085; ENSG00000064703 [Q9UHI6-2] |
GeneIDi | 11218 |
KEGGi | hsa:11218 |
UCSCi | uc001ebs.4, human [Q9UHI6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171063 mRNA Translation: AAF14544.1 AF106019 mRNA Translation: AAD42744.1 AK001506 mRNA Translation: BAA91727.1 AK301697 mRNA Translation: BAG63169.1 AL049557 Genomic DNA No translation available. BC011556 mRNA Translation: AAH11556.1 AL133598 mRNA Translation: CAB63734.2 |
CCDSi | CCDS842.1 [Q9UHI6-1] |
PIRi | T43476 |
RefSeqi | NP_009135.4, NM_007204.4 [Q9UHI6-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OXC | X-ray | 1.30 | A/B | 41-268 | [»] | |
3B7G | X-ray | 1.90 | A/B | 41-268 | [»] | |
SMRi | Q9UHI6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116387, 144 interactors |
ComplexPortali | CPX-6031, SMN complex |
CORUMi | Q9UHI6 |
DIPi | DIP-32606N |
IntActi | Q9UHI6, 66 interactors |
MINTi | Q9UHI6 |
STRINGi | 9606.ENSP00000358716 |
PTM databases
iPTMneti | Q9UHI6 |
PhosphoSitePlusi | Q9UHI6 |
Genetic variation databases
BioMutai | DDX20 |
DMDMi | 12643886 |
Proteomic databases
EPDi | Q9UHI6 |
jPOSTi | Q9UHI6 |
MassIVEi | Q9UHI6 |
PaxDbi | Q9UHI6 |
PeptideAtlasi | Q9UHI6 |
PRIDEi | Q9UHI6 |
ProteomicsDBi | 5385 84357 [Q9UHI6-1] |
Protocols and materials databases
Antibodypediai | 1125, 343 antibodies |
DNASUi | 11218 |
Genome annotation databases
Ensembli | ENST00000369702; ENSP00000358716; ENSG00000064703 [Q9UHI6-1] ENST00000533164; ENSP00000434085; ENSG00000064703 [Q9UHI6-2] |
GeneIDi | 11218 |
KEGGi | hsa:11218 |
UCSCi | uc001ebs.4, human [Q9UHI6-1] |
Organism-specific databases
CTDi | 11218 |
DisGeNETi | 11218 |
GeneCardsi | DDX20 |
HGNCi | HGNC:2743, DDX20 |
HPAi | ENSG00000064703, Tissue enriched (testis) |
MIMi | 606168, gene |
neXtProti | NX_Q9UHI6 |
OpenTargetsi | ENSG00000064703 |
PharmGKBi | PA27209 |
VEuPathDBi | HostDB:ENSG00000064703.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4284, Eukaryota |
GeneTreei | ENSGT00940000158400 |
HOGENOMi | CLU_1854485_0_0_1 |
InParanoidi | Q9UHI6 |
OMAi | AYHMNTV |
PhylomeDBi | Q9UHI6 |
TreeFami | TF352222 |
Enzyme and pathway databases
PathwayCommonsi | Q9UHI6 |
Reactomei | R-HSA-191859, snRNP Assembly |
SIGNORi | Q9UHI6 |
Miscellaneous databases
BioGRID-ORCSi | 11218, 715 hits in 965 CRISPR screens |
ChiTaRSi | DDX20, human |
EvolutionaryTracei | Q9UHI6 |
GeneWikii | DDX20 |
GenomeRNAii | 11218 |
Pharosi | Q9UHI6, Tbio |
PROi | PR:Q9UHI6 |
RNActi | Q9UHI6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000064703, Expressed in testis and 205 other tissues |
ExpressionAtlasi | Q9UHI6, baseline and differential |
Genevisiblei | Q9UHI6, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX20_HUMAN | |
Accessioni | Q9UHI6Primary (citable) accession number: Q9UHI6 Secondary accession number(s): B4DWV7 Q9Y659 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 220 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families