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Protein

Neuronal acetylcholine receptor subunit alpha-9

Gene

CHRNA9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane (PubMed:11752216, PubMed:25282151). The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane (PubMed:11752216, PubMed:25282151). In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion (PubMed:11021840).Curated3 Publications

Miscellaneous

The heterooligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei146Key residue important for potent inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA (AC P0C1D0)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalcium channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Enzyme and pathway databases

ReactomeiR-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-9
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-9
Short name:
NACHR alpha-9
Gene namesi
Name:CHRNA9
Synonyms:NACHRA9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000174343.5
HGNCiHGNC:14079 CHRNA9
MIMi605116 gene
neXtProtiNX_Q9UGM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 237ExtracellularSequence analysisAdd BLAST212
Transmembranei238 – 262HelicalSequence analysisAdd BLAST25
Transmembranei269 – 287HelicalSequence analysisAdd BLAST19
Transmembranei302 – 323HelicalSequence analysisAdd BLAST22
Topological domaini324 – 457CytoplasmicSequence analysisAdd BLAST134
Transmembranei458 – 476HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86I → T: The CHRNA9-CHRNA10 receptor is 250-fold more potently inhibited by the alpha-conotoxin RgIA. 1 Publication1

Organism-specific databases

DisGeNETi55584
OpenTargetsiENSG00000174343
PharmGKBiPA26493

Chemistry databases

ChEMBLiCHEMBL2184
DrugBankiDB05069 ATG003
DB00898 Ethanol
DB00674 Galantamine
DB05137 Lobeline
DB00184 Nicotine
DB05740 RPI-78M
DB08837 Tetraethylammonium
GuidetoPHARMACOLOGYi469

Polymorphism and mutation databases

BioMutaiCHRNA9
DMDMi308153406

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000037126 – 479Neuronal acetylcholine receptor subunit alpha-9Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi155 ↔ 1691 Publication
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi219 ↔ 2201 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9UGM1
PaxDbiQ9UGM1
PeptideAtlasiQ9UGM1
PRIDEiQ9UGM1
ProteomicsDBi84234

PTM databases

iPTMnetiQ9UGM1
PhosphoSitePlusiQ9UGM1

Expressioni

Tissue specificityi

Expressed in cochlea, keratinocytes, pituitary gland, B-cells and T-cells.3 Publications

Gene expression databases

BgeeiENSG00000174343 Expressed in 46 organ(s), highest expression level in zone of skin
CleanExiHS_CHRNA9
GenevisibleiQ9UGM1 HS

Interactioni

Subunit structurei

Can form homo- or heterooligomeric channels in conjunction with CHRNA10 (PubMed:11752216). The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers. Interacts with the alpha-conotoxon RgIA (PubMed:22774872).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P606162EBI-9008641,EBI-16123259From Bungarus multicinctus.

Protein-protein interaction databases

BioGridi120731, 75 interactors
ComplexPortaliCPX-2171 Neuronal nicotinic acetylcholine receptor complex, alpha9-alpha10
CPX-226 Neuronal nicotinic acetylcholine receptor complex, alpha9
CORUMiQ9UGM1
DIPiDIP-61053N
IntActiQ9UGM1, 1 interactor
STRINGi9606.ENSP00000312663

Chemistry databases

BindingDBiQ9UGM1

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UGM1
SMRiQ9UGM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00910000144023
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ9UGM1
KOiK04810
OMAiVEDVEWE
OrthoDBiEOG091G0R20
PhylomeDBiQ9UGM1
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UGM1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNWSHSCISF CWIYFAASRL RAAETADGKY AQKLFNDLFE DYSNALRPVE
60 70 80 90 100
DTDKVLNVTL QITLSQIKDM DERNQILTAY LWIRQIWHDA YLTWDRDQYD
110 120 130 140 150
GLDSIRIPSD LVWRPDIVLY NKADDESSEP VNTNVVLRYD GLITWDAPAI
160 170 180 190 200
TKSSCVVDVT YFPFDNQQCN LTFGSWTYNG NQVDIFNALD SGDLSDFIED
210 220 230 240 250
VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY IVNLLIPCVL
260 270 280 290 300
ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG
310 320 330 340 350
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWARVV ILKYMSRVLF
360 370 380 390 400
VYDVGESCLS PHHSRERDHL TKVYSKLPES NLKAARNKDL SRKKDMNKRL
410 420 430 440 450
KNDLGCQGKN PQEAESYCAQ YKVLTRNIEY IAKCLKDHKA TNSKGSEWKK
460 470
VAKVIDRFFM WIFFIMVFVM TILIIARAD
Length:479
Mass (Da):54,807
Last modified:October 5, 2010 - v2
Checksum:iAA6B46B559D6111D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03115196R → Q. Corresponds to variant dbSNP:rs10024518Ensembl.1
Natural variantiVAR_060996315A → V. Corresponds to variant dbSNP:rs55633891Ensembl.1
Natural variantiVAR_025425442N → S3 PublicationsCorresponds to variant dbSNP:rs10009228Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243342 mRNA Translation: CAB65091.1
AY123244 Genomic DNA Translation: AAM74523.1
AC118275 Genomic DNA Translation: AAY40986.1
BC113549 mRNA Translation: AAI13550.1
BC113575 mRNA Translation: AAI13576.1
AF227732 mRNA Translation: AAF61920.1
CCDSiCCDS3459.1
RefSeqiNP_060051.2, NM_017581.3
UniGeneiHs.272278

Genome annotation databases

EnsembliENST00000310169; ENSP00000312663; ENSG00000174343
GeneIDi55584
KEGGihsa:55584
UCSCiuc003gva.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243342 mRNA Translation: CAB65091.1
AY123244 Genomic DNA Translation: AAM74523.1
AC118275 Genomic DNA Translation: AAY40986.1
BC113549 mRNA Translation: AAI13550.1
BC113575 mRNA Translation: AAI13576.1
AF227732 mRNA Translation: AAF61920.1
CCDSiCCDS3459.1
RefSeqiNP_060051.2, NM_017581.3
UniGeneiHs.272278

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D01X-ray1.80A26-237[»]
4UXUX-ray1.71A/B26-237[»]
4UY2X-ray2.70A/B26-237[»]
ProteinModelPortaliQ9UGM1
SMRiQ9UGM1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120731, 75 interactors
ComplexPortaliCPX-2171 Neuronal nicotinic acetylcholine receptor complex, alpha9-alpha10
CPX-226 Neuronal nicotinic acetylcholine receptor complex, alpha9
CORUMiQ9UGM1
DIPiDIP-61053N
IntActiQ9UGM1, 1 interactor
STRINGi9606.ENSP00000312663

Chemistry databases

BindingDBiQ9UGM1
ChEMBLiCHEMBL2184
DrugBankiDB05069 ATG003
DB00898 Ethanol
DB00674 Galantamine
DB05137 Lobeline
DB00184 Nicotine
DB05740 RPI-78M
DB08837 Tetraethylammonium
GuidetoPHARMACOLOGYi469

PTM databases

iPTMnetiQ9UGM1
PhosphoSitePlusiQ9UGM1

Polymorphism and mutation databases

BioMutaiCHRNA9
DMDMi308153406

Proteomic databases

MaxQBiQ9UGM1
PaxDbiQ9UGM1
PeptideAtlasiQ9UGM1
PRIDEiQ9UGM1
ProteomicsDBi84234

Protocols and materials databases

DNASUi55584
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310169; ENSP00000312663; ENSG00000174343
GeneIDi55584
KEGGihsa:55584
UCSCiuc003gva.2 human

Organism-specific databases

CTDi55584
DisGeNETi55584
EuPathDBiHostDB:ENSG00000174343.5
GeneCardsiCHRNA9
H-InvDBiHIX0031380
HGNCiHGNC:14079 CHRNA9
MIMi605116 gene
neXtProtiNX_Q9UGM1
OpenTargetsiENSG00000174343
PharmGKBiPA26493
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00910000144023
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ9UGM1
KOiK04810
OMAiVEDVEWE
OrthoDBiEOG091G0R20
PhylomeDBiQ9UGM1
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors

Miscellaneous databases

ChiTaRSiCHRNA9 human
GeneWikiiCHRNA9
GenomeRNAii55584
PROiPR:Q9UGM1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174343 Expressed in 46 organ(s), highest expression level in zone of skin
CleanExiHS_CHRNA9
GenevisibleiQ9UGM1 HS

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHA9_HUMAN
AccessioniPrimary (citable) accession number: Q9UGM1
Secondary accession number(s): Q14CY7, Q4W5A2, Q9NYV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 5, 2010
Last modified: November 7, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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