UniProtKB - Q9UGI8 (TES_HUMAN)
Testin
TES
Functioni
Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.
3 PublicationsGO - Molecular functioni
- cadherin binding Source: BHF-UCL
- RNA binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- negative regulation of cell population proliferation Source: UniProtKB
Keywordsi
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9UGI8 |
SignaLinki | Q9UGI8 |
Names & Taxonomyi
Protein namesi | Recommended name: TestinAlternative name(s): TESS |
Gene namesi | Name:TES |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14620, TES |
MIMi | 606085, gene |
neXtProti | NX_Q9UGI8 |
VEuPathDBi | HostDB:ENSG00000135269 |
Subcellular locationi
Cytoplasm and Cytosol
Other locations
Note: Detected along actin stress fibers.
Cytosol
- cytosol Source: HPA
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cell junction Source: HPA
- focal adhesion Source: HPA
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 328 | C → A: Abolishes interaction with ACTL7A. 1 Publication | 1 | |
Mutagenesisi | 391 | C → A: Abolishes localization at focal adhesions. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 26136 |
OpenTargetsi | ENSG00000135269 |
PharmGKBi | PA37906 |
Miscellaneous databases
Pharosi | Q9UGI8, Tbio |
Genetic variation databases
BioMutai | TES |
DMDMi | 17380320 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075906 | 1 – 421 | TestinAdd BLAST | 421 |
Proteomic databases
jPOSTi | Q9UGI8 |
MassIVEi | Q9UGI8 |
MaxQBi | Q9UGI8 |
PaxDbi | Q9UGI8 |
PeptideAtlasi | Q9UGI8 |
PRIDEi | Q9UGI8 |
ProteomicsDBi | 84218 [Q9UGI8-1] 84219 [Q9UGI8-2] |
PTM databases
GlyGeni | Q9UGI8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9UGI8 |
MetOSitei | Q9UGI8 |
PhosphoSitePlusi | Q9UGI8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000135269, Expressed in amniotic fluid and 233 other tissues |
ExpressionAtlasi | Q9UGI8, baseline and differential |
Genevisiblei | Q9UGI8, HS |
Organism-specific databases
HPAi | ENSG00000135269, Tissue enhanced (seminal) |
Interactioni
Subunit structurei
Interacts via LIM domain 1 with ZYX.
Interacts (via LIM domain 3) with ENAH and VASP.
Interacts with ALKBH4, talin, actin, alpha-actinin, GRIP1 and PXN.
Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer.
5 PublicationsBinary interactionsi
Q9UGI8
With | #Exp. | IntAct |
---|---|---|
ACTL7A [Q9Y615] | 9 | EBI-2561654,EBI-10825302 |
ENAH [Q8N8S7] | 2 | EBI-2561654,EBI-2834410 |
SPRED1 [Q7Z699] | 3 | EBI-2561654,EBI-5235340 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 117572, 308 interactors |
IntActi | Q9UGI8, 57 interactors |
MINTi | Q9UGI8 |
STRINGi | 9606.ENSP00000350937 |
Miscellaneous databases
RNActi | Q9UGI8, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UGI8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UGI8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 92 – 199 | PETPROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 234 – 297 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 299 – 359 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 362 – 421 | LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST | 60 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 133 – 164 | DisorderedSequence analysisAdd BLAST | 32 |
Domaini
Sequence similaritiesi
Keywords - Domaini
LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG1704, Eukaryota |
GeneTreei | ENSGT00940000155993 |
HOGENOMi | CLU_008937_1_1_1 |
InParanoidi | Q9UGI8 |
OMAi | YVMVNEK |
PhylomeDBi | Q9UGI8 |
TreeFami | TF313265 |
Family and domain databases
CDDi | cd09413, LIM1_Testin, 1 hit cd09416, LIM2_Testin, 1 hit cd09419, LIM3_Testin, 1 hit cd09829, PET_testin, 1 hit |
IDEALi | IID00684 |
InterProi | View protein in InterPro IPR034958, LIM1_Testin IPR034959, LIM2_Testin IPR034960, LIM3_Testin IPR010442, PET_domain IPR033724, PET_testin IPR027683, Testin IPR001781, Znf_LIM |
PANTHERi | PTHR24211:SF1, PTHR24211:SF1, 1 hit |
Pfami | View protein in Pfam PF00412, LIM, 2 hits PF06297, PET, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 3 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS51303, PET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE
60 70 80 90 100
HDVLLSNEED RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA
110 120 130 140 150
KKNVSINTVT YEWAPPVQNQ ALARQYMQML PKEKQPVAGS EGAQYRKKQL
160 170 180 190 200
AKQLPAHDQD PSKCHELSPR EVKEMEQFVK KYKSEALGVG DVKLPCEMDA
210 220 230 240 250
QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK LSMKEGDPAI
260 270 280 290 300
YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR
310 320 330 340 350
CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK
360 370 380 390 400
PCYVKNHAVV CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG
410 420
QKFMPVEGMV FCSVECKKRM S
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7BYK1 | H7BYK1_HUMAN | Testin | TES | 192 | Annotation score: | ||
F8W7T0 | F8W7T0_HUMAN | Testin | TES | 79 | Annotation score: | ||
F8WDI4 | F8WDI4_HUMAN | Testin | TES | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | K → E in BAB13846 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050170 | 221 | A → V. Corresponds to variant dbSNP:rs2272193Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003122 | 1 – 9 | Missing in isoform 2. 2 Publications | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260225 Genomic DNA Translation: AAG17635.1 AF260225 Genomic DNA Translation: AAG17636.1 AF245356 mRNA Translation: AAG17612.1 AF245357 mRNA Translation: AAG17613.1 AJ250865 mRNA Translation: CAB65119.1 AK021575 mRNA Translation: BAB13846.1 AK291802 mRNA Translation: BAF84491.1 AC073130 Genomic DNA Translation: AAQ93367.1 CH236947 Genomic DNA Translation: EAL24365.1 BC001451 mRNA Translation: AAH01451.1 |
CCDSi | CCDS5763.1 [Q9UGI8-1] CCDS5764.1 [Q9UGI8-2] |
RefSeqi | NP_056456.1, NM_015641.3 [Q9UGI8-1] NP_690042.1, NM_152829.2 [Q9UGI8-2] |
Genome annotation databases
Ensembli | ENST00000358204; ENSP00000350937; ENSG00000135269 ENST00000393481; ENSP00000377121; ENSG00000135269 [Q9UGI8-2] |
GeneIDi | 26136 |
KEGGi | hsa:26136 |
MANE-Selecti | ENST00000358204.9; ENSP00000350937.4; NM_015641.4; NP_056456.1 |
UCSCi | uc003vho.3, human [Q9UGI8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260225 Genomic DNA Translation: AAG17635.1 AF260225 Genomic DNA Translation: AAG17636.1 AF245356 mRNA Translation: AAG17612.1 AF245357 mRNA Translation: AAG17613.1 AJ250865 mRNA Translation: CAB65119.1 AK021575 mRNA Translation: BAB13846.1 AK291802 mRNA Translation: BAF84491.1 AC073130 Genomic DNA Translation: AAQ93367.1 CH236947 Genomic DNA Translation: EAL24365.1 BC001451 mRNA Translation: AAH01451.1 |
CCDSi | CCDS5763.1 [Q9UGI8-1] CCDS5764.1 [Q9UGI8-2] |
RefSeqi | NP_056456.1, NM_015641.3 [Q9UGI8-1] NP_690042.1, NM_152829.2 [Q9UGI8-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IYB | X-ray | 2.35 | E/F/G/H | 357-421 | [»] | |
2XQN | X-ray | 2.62 | T | 296-421 | [»] | |
SMRi | Q9UGI8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117572, 308 interactors |
IntActi | Q9UGI8, 57 interactors |
MINTi | Q9UGI8 |
STRINGi | 9606.ENSP00000350937 |
PTM databases
GlyGeni | Q9UGI8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9UGI8 |
MetOSitei | Q9UGI8 |
PhosphoSitePlusi | Q9UGI8 |
Genetic variation databases
BioMutai | TES |
DMDMi | 17380320 |
Proteomic databases
jPOSTi | Q9UGI8 |
MassIVEi | Q9UGI8 |
MaxQBi | Q9UGI8 |
PaxDbi | Q9UGI8 |
PeptideAtlasi | Q9UGI8 |
PRIDEi | Q9UGI8 |
ProteomicsDBi | 84218 [Q9UGI8-1] 84219 [Q9UGI8-2] |
Protocols and materials databases
Antibodypediai | 2972, 192 antibodies from 26 providers |
DNASUi | 26136 |
Genome annotation databases
Ensembli | ENST00000358204; ENSP00000350937; ENSG00000135269 ENST00000393481; ENSP00000377121; ENSG00000135269 [Q9UGI8-2] |
GeneIDi | 26136 |
KEGGi | hsa:26136 |
MANE-Selecti | ENST00000358204.9; ENSP00000350937.4; NM_015641.4; NP_056456.1 |
UCSCi | uc003vho.3, human [Q9UGI8-1] |
Organism-specific databases
CTDi | 26136 |
DisGeNETi | 26136 |
GeneCardsi | TES |
HGNCi | HGNC:14620, TES |
HPAi | ENSG00000135269, Tissue enhanced (seminal) |
MIMi | 606085, gene |
neXtProti | NX_Q9UGI8 |
OpenTargetsi | ENSG00000135269 |
PharmGKBi | PA37906 |
VEuPathDBi | HostDB:ENSG00000135269 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1704, Eukaryota |
GeneTreei | ENSGT00940000155993 |
HOGENOMi | CLU_008937_1_1_1 |
InParanoidi | Q9UGI8 |
OMAi | YVMVNEK |
PhylomeDBi | Q9UGI8 |
TreeFami | TF313265 |
Enzyme and pathway databases
PathwayCommonsi | Q9UGI8 |
SignaLinki | Q9UGI8 |
Miscellaneous databases
BioGRID-ORCSi | 26136, 6 hits in 1042 CRISPR screens |
ChiTaRSi | TES, human |
EvolutionaryTracei | Q9UGI8 |
GeneWikii | Testin |
GenomeRNAii | 26136 |
Pharosi | Q9UGI8, Tbio |
PROi | PR:Q9UGI8 |
RNActi | Q9UGI8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000135269, Expressed in amniotic fluid and 233 other tissues |
ExpressionAtlasi | Q9UGI8, baseline and differential |
Genevisiblei | Q9UGI8, HS |
Family and domain databases
CDDi | cd09413, LIM1_Testin, 1 hit cd09416, LIM2_Testin, 1 hit cd09419, LIM3_Testin, 1 hit cd09829, PET_testin, 1 hit |
IDEALi | IID00684 |
InterProi | View protein in InterPro IPR034958, LIM1_Testin IPR034959, LIM2_Testin IPR034960, LIM3_Testin IPR010442, PET_domain IPR033724, PET_testin IPR027683, Testin IPR001781, Znf_LIM |
PANTHERi | PTHR24211:SF1, PTHR24211:SF1, 1 hit |
Pfami | View protein in Pfam PF00412, LIM, 2 hits PF06297, PET, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 3 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 3 hits PS51303, PET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TES_HUMAN | |
Accessioni | Q9UGI8Primary (citable) accession number: Q9UGI8 Secondary accession number(s): A4D0U6, Q9GZQ1, Q9HAJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families