UniProtKB - Q9UGI0 (ZRAN1_HUMAN)
Ubiquitin thioesterase ZRANB1
ZRANB1
Functioni
Ubiquitin thioesterase, which specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin (PubMed:22157957, PubMed:23827681, PubMed:25752573, PubMed:25752577).
Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones (PubMed:18281465).
Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription (PubMed:18281465).
Acts as a regulator of autophagy by mediating deubiquitination of PIK3C3/VPS34, thereby promoting autophagosome maturation (PubMed:33637724).
Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed:21834987).
Required in the stress fiber dynamics and cell migration (PubMed:21834987).
7 PublicationsCatalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).4 Publications EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 10 | Zinc 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 13 | Zinc 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 24 | Zinc 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 27 | Zinc 1PROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 90 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 93 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 104 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 107 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 155 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 158 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 169 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 172 | Zinc 3PROSITE-ProRule annotation | 1 | |
Active sitei | 443 | Nucleophile1 Publication | 1 | |
Active sitei | 585 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 33 | RanBP2-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 84 – 113 | RanBP2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 149 – 178 | RanBP2-type 3PROSITE-ProRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- deubiquitinase activity Source: UniProtKB
- K63-linked polyubiquitin modification-dependent protein binding Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- thiol-dependent deubiquitinase Source: UniProtKB
GO - Biological processi
- cell migration Source: UniProtKB
- cytoskeleton organization Source: UniProtKB
- positive regulation of Wnt signaling pathway Source: UniProtKB
- protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: BHF-UCL
- protein K29-linked deubiquitination Source: UniProtKB
- protein K33-linked deubiquitination Source: UniProtKB
- protein K63-linked deubiquitination Source: UniProtKB
- regulation of cell morphogenesis Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9UGI0 |
Reactomei | R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q9UGI0 |
Protein family/group databases
MEROPSi | C64.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin thioesterase ZRANB1Curated (EC:3.4.19.124 Publications)Alternative name(s): TRAF-binding domain-containing protein1 Publication Short name: hTrabid1 Publication Zinc finger Ran-binding domain-containing protein 11 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18224, ZRANB1 |
MIMi | 611749, gene |
neXtProti | NX_Q9UGI0 |
VEuPathDBi | HostDB:ENSG00000019995 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | C → A: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with 14-LV-15; A-90; 94-LV-95; A-155 and 159-LV-160. 1 Publication | 1 | |
Mutagenesisi | 12 | Y → A: Does not affect binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 14 – 15 | TY → LV: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with A-10; A-90; 94-LV-95; A-155 and 159-LV-160. 1 Publication | 2 | |
Mutagenesisi | 14 | T → A: Abolished binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 15 | Y → F: Strongly reduced binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 2 Publications | 1 | |
Mutagenesisi | 16 | E → A: Does not affect binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 18 | W → A: Abolished binding to 'Lys-33'-linked diubiquitin. 1 Publication | 1 | |
Mutagenesisi | 20 | S → R: Strongly reduced binding to 'Lys-33'-linked diubiquitin. 1 Publication | 1 | |
Mutagenesisi | 25 | T → A: Does not affect binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 25 | T → D: Abolished binding to 'Lys-33'-linked diubiquitin. 1 Publication | 1 | |
Mutagenesisi | 26 | M → A: Abolished binding to 'Lys-29'- and 'Lys-33'-linked ubiquitin. 1 Publication | 1 | |
Mutagenesisi | 90 | C → A: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with A-10; 14-LV-15; 94-LV-95; A-155 and 159-LV-160. 1 Publication | 1 | |
Mutagenesisi | 94 – 95 | TY → LV: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with A-10; 14-LV-15; A-90; A-155 and 159-LV-160. 1 Publication | 2 | |
Mutagenesisi | 155 | C → A: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with A-10; 14-LV-15; A-90; 94-LV-95 and 159-LV-160. 1 Publication | 1 | |
Mutagenesisi | 159 – 160 | TY → LV: Abolishes the binding to ubiquitin chains but not the deubiquitinating activity; when associated with A-10; 14-LV-15; A-90; 94-LV-95; and A-155. 1 Publication | 2 | |
Mutagenesisi | 443 | C → S: Abolishes the deubiquitinating activity but not the binding to ubiquitin chains. 3 Publications | 1 |
Organism-specific databases
DisGeNETi | 54764 |
OpenTargetsi | ENSG00000019995 |
PharmGKBi | PA134933584 |
Miscellaneous databases
Pharosi | Q9UGI0, Tbio |
Genetic variation databases
BioMutai | ZRANB1 |
DMDMi | 212276487 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065595 | 1 – 708 | Ubiquitin thioesterase ZRANB1Add BLAST | 708 |
Proteomic databases
EPDi | Q9UGI0 |
jPOSTi | Q9UGI0 |
MassIVEi | Q9UGI0 |
MaxQBi | Q9UGI0 |
PaxDbi | Q9UGI0 |
PeptideAtlasi | Q9UGI0 |
PRIDEi | Q9UGI0 |
ProteomicsDBi | 84215 |
PTM databases
iPTMneti | Q9UGI0 |
PhosphoSitePlusi | Q9UGI0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000019995, Expressed in gastrocnemius and 218 other tissues |
Genevisiblei | Q9UGI0, HS |
Organism-specific databases
HPAi | ENSG00000019995, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Q9UGI0
GO - Molecular functioni
- K63-linked polyubiquitin modification-dependent protein binding Source: BHF-UCL
- polyubiquitin modification-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 120139, 189 interactors |
DIPi | DIP-33806N |
IntActi | Q9UGI0, 168 interactors |
MINTi | Q9UGI0 |
STRINGi | 9606.ENSP00000352676 |
Miscellaneous databases
RNActi | Q9UGI0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UGI0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 260 – 290 | ANK 1Add BLAST | 31 | |
Repeati | 313 – 340 | ANK 2Add BLAST | 28 | |
Domaini | 432 – 592 | OTUPROSITE-ProRule annotationAdd BLAST | 161 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 38 – 73 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 200 – 225 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 392 – 641 | TRAF-binding1 PublicationAdd BLAST | 250 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 50 – 65 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 202 – 217 | Polar residuesSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 33 | RanBP2-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 84 – 113 | RanBP2-type 2PROSITE-ProRule annotationAdd BLAST | 30 | |
Zinc fingeri | 149 – 178 | RanBP2-type 3PROSITE-ProRule annotationAdd BLAST | 30 |
Keywords - Domaini
ANK repeat, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000158045 |
HOGENOMi | CLU_013907_0_0_1 |
InParanoidi | Q9UGI0 |
OMAi | YPRWKEY |
OrthoDBi | 728724at2759 |
PhylomeDBi | Q9UGI0 |
TreeFami | TF323312 |
Family and domain databases
InterProi | View protein in InterPro IPR041294, AnkUBD IPR003323, OTU_dom IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF18418, AnkUBD, 1 hit PF02338, OTU, 1 hit PF00641, zf-RanBP, 2 hits |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 3 hits |
SUPFAMi | SSF90209, SSF90209, 2 hits |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS01358, ZF_RANBP2_1, 3 hits PS50199, ZF_RANBP2_2, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSERGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG
60 70 80 90 100
RDWDPSSTEG GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR
110 120 130 140 150
AIRCTQCLSQ RRTRSPTESP QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT
160 170 180 190 200
QHWTCSVCTY ENWAKAKRCV VCDHPRPNNI EAIELAETEE ASSIINEQDR
210 220 230 240 250
ARWRGSCSSG NSQRRSPPAT KRDSEVKMDF QRIELAGAVG SKEELEVDFK
260 270 280 290 300
KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV
310 320 330 340 350
RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE
360 370 380 390 400
LTEQIRREIA ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD
410 420 430 440 450
EVLDRDVQKE LEEESPIINW SLELATRLDS RLYALWNRTA GDCLLDSVLQ
460 470 480 490 500
ATWGIYDKDS VLRKALHDSL HDCSHWFYTR WKDWESWYSQ SFGLHFSLRE
510 520 530 540 550
EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY GVKYYKSFRG
560 570 580 590 600
ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR
610 620 630 640 650
GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW
660 670 680 690 700
LDCCVTEGGV LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE
EDEDDEDE
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 374 | F → I in CAB64449 (PubMed:11463333).Curated | 1 | |
Sequence conflicti | 384 | P → A in CAB64449 (PubMed:11463333).Curated | 1 | |
Sequence conflicti | 602 | A → P in BAG64010 (PubMed:14702039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ252060 mRNA Translation: CAB64449.1 AK302810 mRNA Translation: BAG64010.1 Different initiation. AL731577 Genomic DNA No translation available. AL731571 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49252.1 CH471066 Genomic DNA Translation: EAW49253.1 AL832925 mRNA Translation: CAH10620.1 |
CCDSi | CCDS7642.1 |
RefSeqi | NP_060050.2, NM_017580.2 XP_005269983.1, XM_005269926.4 XP_016871846.1, XM_017016357.1 |
Genome annotation databases
Ensembli | ENST00000359653; ENSP00000352676; ENSG00000019995 |
GeneIDi | 54764 |
KEGGi | hsa:54764 |
MANE-Selecti | ENST00000359653.4; ENSP00000352676.4; NM_017580.3; NP_060050.2 |
UCSCi | uc001lic.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ252060 mRNA Translation: CAB64449.1 AK302810 mRNA Translation: BAG64010.1 Different initiation. AL731577 Genomic DNA No translation available. AL731571 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49252.1 CH471066 Genomic DNA Translation: EAW49253.1 AL832925 mRNA Translation: CAH10620.1 |
CCDSi | CCDS7642.1 |
RefSeqi | NP_060050.2, NM_017580.2 XP_005269983.1, XM_005269926.4 XP_016871846.1, XM_017016357.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZRH | X-ray | 2.23 | A | 245-697 | [»] | |
5AF6 | X-ray | 3.40 | F/G/H/I/J | 1-33 | [»] | |
SMRi | Q9UGI0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120139, 189 interactors |
DIPi | DIP-33806N |
IntActi | Q9UGI0, 168 interactors |
MINTi | Q9UGI0 |
STRINGi | 9606.ENSP00000352676 |
Protein family/group databases
MEROPSi | C64.004 |
PTM databases
iPTMneti | Q9UGI0 |
PhosphoSitePlusi | Q9UGI0 |
Genetic variation databases
BioMutai | ZRANB1 |
DMDMi | 212276487 |
Proteomic databases
EPDi | Q9UGI0 |
jPOSTi | Q9UGI0 |
MassIVEi | Q9UGI0 |
MaxQBi | Q9UGI0 |
PaxDbi | Q9UGI0 |
PeptideAtlasi | Q9UGI0 |
PRIDEi | Q9UGI0 |
ProteomicsDBi | 84215 |
Protocols and materials databases
Antibodypediai | 35158, 164 antibodies from 27 providers |
DNASUi | 54764 |
Genome annotation databases
Ensembli | ENST00000359653; ENSP00000352676; ENSG00000019995 |
GeneIDi | 54764 |
KEGGi | hsa:54764 |
MANE-Selecti | ENST00000359653.4; ENSP00000352676.4; NM_017580.3; NP_060050.2 |
UCSCi | uc001lic.4, human |
Organism-specific databases
CTDi | 54764 |
DisGeNETi | 54764 |
GeneCardsi | ZRANB1 |
HGNCi | HGNC:18224, ZRANB1 |
HPAi | ENSG00000019995, Low tissue specificity |
MIMi | 611749, gene |
neXtProti | NX_Q9UGI0 |
OpenTargetsi | ENSG00000019995 |
PharmGKBi | PA134933584 |
VEuPathDBi | HostDB:ENSG00000019995 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000158045 |
HOGENOMi | CLU_013907_0_0_1 |
InParanoidi | Q9UGI0 |
OMAi | YPRWKEY |
OrthoDBi | 728724at2759 |
PhylomeDBi | Q9UGI0 |
TreeFami | TF323312 |
Enzyme and pathway databases
PathwayCommonsi | Q9UGI0 |
Reactomei | R-HSA-195253, Degradation of beta-catenin by the destruction complex R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q9UGI0 |
Miscellaneous databases
BioGRID-ORCSi | 54764, 33 hits in 1093 CRISPR screens |
ChiTaRSi | ZRANB1, human |
GenomeRNAii | 54764 |
Pharosi | Q9UGI0, Tbio |
PROi | PR:Q9UGI0 |
RNActi | Q9UGI0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000019995, Expressed in gastrocnemius and 218 other tissues |
Genevisiblei | Q9UGI0, HS |
Family and domain databases
InterProi | View protein in InterPro IPR041294, AnkUBD IPR003323, OTU_dom IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF18418, AnkUBD, 1 hit PF02338, OTU, 1 hit PF00641, zf-RanBP, 2 hits |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 3 hits |
SUPFAMi | SSF90209, SSF90209, 2 hits |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS01358, ZF_RANBP2_1, 3 hits PS50199, ZF_RANBP2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZRAN1_HUMAN | |
Accessioni | Q9UGI0Primary (citable) accession number: Q9UGI0 Secondary accession number(s): B4DZ98 Q69YK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 4, 2008 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families