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Protein

Vesicle transport through interaction with t-SNAREs homolog 1B

Gene

VTI1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.1 Publication

GO - Molecular functioni

  • chloride channel inhibitor activity Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: MGI

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation

Protein family/group databases

TCDBi1.F.1.1.5 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle transport through interaction with t-SNAREs homolog 1B
Alternative name(s):
Vesicle transport v-SNARE protein Vti1-like 1
Vti1-rp1
Gene namesi
Name:VTI1B
Synonyms:VTI1, VTI1L, VTI1L1, VTI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100568.10
HGNCiHGNC:17793 VTI1B
MIMi603207 gene
neXtProtiNX_Q9UEU0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 208CytoplasmicSequence analysisAdd BLAST207
Transmembranei209 – 229Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini230 – 232VesicularSequence analysis3

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8S → W: Abolished binding to CLINT1. 1 Publication1
Mutagenesisi12E → A: Abolished binding to CLINT1. Abnormal subcellular localization restricted to late endosomes and lysosomes. 1 Publication1
Mutagenesisi17I → S: Normal binding to CLINT1. 1 Publication1
Mutagenesisi23E → R: Abolished binding to CLINT1. Rescued binding to CLINT1 E-146 mutant. 1 Publication1
Mutagenesisi65E → W: Abolished binding to CLINT1. 1 Publication1
Mutagenesisi73F → S: Abolished binding to CLINT1. Abnormal subcellular localization restricted to late endosomes and lysosomes. 1 Publication1
Mutagenesisi76P → S: Reduced binding to CLINT1. 1 Publication1
Mutagenesisi77M → S: Normal binding to CLINT1. 1 Publication1

Organism-specific databases

DisGeNETi10490
OpenTargetsiENSG00000100568
PharmGKBiPA134861090

Polymorphism and mutation databases

BioMutaiVTI1B
DMDMi126302613

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002182282 – 232Vesicle transport through interaction with t-SNAREs homolog 1BAdd BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei103PhosphothreonineCombined sources1
Modified residuei107Omega-N-methylarginineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9UEU0
PaxDbiQ9UEU0
PeptideAtlasiQ9UEU0
PRIDEiQ9UEU0
ProteomicsDBi84155
84156 [Q9UEU0-2]
TopDownProteomicsiQ9UEU0-1 [Q9UEU0-1]
Q9UEU0-2 [Q9UEU0-2]

PTM databases

iPTMnetiQ9UEU0
PhosphoSitePlusiQ9UEU0
SwissPalmiQ9UEU0

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiENSG00000100568 Expressed in 230 organ(s), highest expression level in C1 segment of cervical spinal cord
ExpressionAtlasiQ9UEU0 baseline and differential
GenevisibleiQ9UEU0 HS

Organism-specific databases

HPAiHPA044121
HPA048150
HPA060118

Interactioni

Subunit structurei

Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17 (PubMed:23217709). Interacts with CLINT1 (PubMed:18033301).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115753, 98 interactors
CORUMiQ9UEU0
IntActiQ9UEU0, 39 interactors
MINTiQ9UEU0
STRINGi9606.ENSP00000450731

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UEU0
SMRiQ9UEU0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UEU0

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 23Interaction with CLINT1Combined sources1 PublicationAdd BLAST22
Regioni69 – 73Interaction with CLINT1Combined sources1 Publication5

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili35 – 98Sequence analysisAdd BLAST64
Coiled coili161 – 198Sequence analysisAdd BLAST38

Sequence similaritiesi

Belongs to the VTI1 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1666 Eukaryota
ENOG4111J90 LUCA
GeneTreeiENSGT00530000063466
HOGENOMiHOG000116573
HOVERGENiHBG058837
InParanoidiQ9UEU0
KOiK08493
OMAiEELKYAP
OrthoDBiEOG091G12XX
PhylomeDBiQ9UEU0
TreeFamiTF312874

Family and domain databases

Gene3Di1.20.58.400, 1 hit
InterProiView protein in InterPro
IPR010989 SNARE
IPR000727 T_SNARE_dom
IPR038407 v-SNARE_N_sf
IPR007705 Vesicle_trsprt_v-SNARE_N
PfamiView protein in Pfam
PF05008 V-SNARE, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: Q9UEU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASSAASSEH FEKLHEIFRG LHEDLQGVPE RLLGTAGTEE KKKLIRDFDE
60 70 80 90 100
KQQEANETLA EMEEELRYAP LSFRNPMMSK LRNYRKDLAK LHREVRSTPL
110 120 130 140 150
TATPGGRGDM KYGIYAVENE HMNRLQSQRA MLLQGTESLN RATQSIERSH
160 170 180 190 200
RIATETDQIG SEIIEELGEQ RDQLERTKSR LVNTSENLSK SRKILRSMSR
210 220 230
KVTTNKLLLS IIILLELAIL GGLVYYKFFR SH
Length:232
Mass (Da):26,688
Last modified:February 20, 2007 - v3
Checksum:iB421C2863235B9B1
GO
Isoform Short (identifier: Q9UEU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:171
Mass (Da):19,810
Checksum:i7BC64529E0DBE12C
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YJJ5H0YJJ5_HUMAN
Vesicle transport through interacti...
VTI1B
128Annotation score:
H0YJL5H0YJL5_HUMAN
Vesicle transport through interacti...
VTI1B
103Annotation score:
G3V5I2G3V5I2_HUMAN
Vesicle transport through interacti...
VTI1B
59Annotation score:
J3KMW2J3KMW2_HUMAN
Vesicle transport through interacti...
VTI1B
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24D → N in AAC52016 (PubMed:9446565).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0067531 – 61Missing in isoform Short. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA Translation: AAC52016.1
AF060902 mRNA Translation: AAC73059.1
CR456757 mRNA Translation: CAG33038.1
CR542095 mRNA Translation: CAG46892.1
BT019348 mRNA Translation: AAV38155.1
BC003142 mRNA Translation: AAH03142.1
CCDSiCCDS9786.1 [Q9UEU0-1]
RefSeqiNP_006361.1, NM_006370.2 [Q9UEU0-1]
UniGeneiHs.741177

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568 [Q9UEU0-1]
GeneIDi10490
KEGGihsa:10490
UCSCiuc001xjt.4 human [Q9UEU0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035824 mRNA Translation: AAC52016.1
AF060902 mRNA Translation: AAC73059.1
CR456757 mRNA Translation: CAG33038.1
CR542095 mRNA Translation: CAG46892.1
BT019348 mRNA Translation: AAV38155.1
BC003142 mRNA Translation: AAH03142.1
CCDSiCCDS9786.1 [Q9UEU0-1]
RefSeqiNP_006361.1, NM_006370.2 [Q9UEU0-1]
UniGeneiHs.741177

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V8SX-ray2.22V1-96[»]
ProteinModelPortaliQ9UEU0
SMRiQ9UEU0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115753, 98 interactors
CORUMiQ9UEU0
IntActiQ9UEU0, 39 interactors
MINTiQ9UEU0
STRINGi9606.ENSP00000450731

Protein family/group databases

TCDBi1.F.1.1.5 the synaptosomal vesicle fusion pore (svf-pore) family

PTM databases

iPTMnetiQ9UEU0
PhosphoSitePlusiQ9UEU0
SwissPalmiQ9UEU0

Polymorphism and mutation databases

BioMutaiVTI1B
DMDMi126302613

Proteomic databases

EPDiQ9UEU0
PaxDbiQ9UEU0
PeptideAtlasiQ9UEU0
PRIDEiQ9UEU0
ProteomicsDBi84155
84156 [Q9UEU0-2]
TopDownProteomicsiQ9UEU0-1 [Q9UEU0-1]
Q9UEU0-2 [Q9UEU0-2]

Protocols and materials databases

DNASUi10490
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000554659; ENSP00000450731; ENSG00000100568 [Q9UEU0-1]
GeneIDi10490
KEGGihsa:10490
UCSCiuc001xjt.4 human [Q9UEU0-1]

Organism-specific databases

CTDi10490
DisGeNETi10490
EuPathDBiHostDB:ENSG00000100568.10
GeneCardsiVTI1B
HGNCiHGNC:17793 VTI1B
HPAiHPA044121
HPA048150
HPA060118
MIMi603207 gene
neXtProtiNX_Q9UEU0
OpenTargetsiENSG00000100568
PharmGKBiPA134861090
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1666 Eukaryota
ENOG4111J90 LUCA
GeneTreeiENSGT00530000063466
HOGENOMiHOG000116573
HOVERGENiHBG058837
InParanoidiQ9UEU0
KOiK08493
OMAiEELKYAP
OrthoDBiEOG091G12XX
PhylomeDBiQ9UEU0
TreeFamiTF312874

Enzyme and pathway databases

ReactomeiR-HSA-114608 Platelet degranulation

Miscellaneous databases

ChiTaRSiVTI1B human
EvolutionaryTraceiQ9UEU0
GeneWikiiVTI1B
GenomeRNAii10490
PROiPR:Q9UEU0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100568 Expressed in 230 organ(s), highest expression level in C1 segment of cervical spinal cord
ExpressionAtlasiQ9UEU0 baseline and differential
GenevisibleiQ9UEU0 HS

Family and domain databases

Gene3Di1.20.58.400, 1 hit
InterProiView protein in InterPro
IPR010989 SNARE
IPR000727 T_SNARE_dom
IPR038407 v-SNARE_N_sf
IPR007705 Vesicle_trsprt_v-SNARE_N
PfamiView protein in Pfam
PF05008 V-SNARE, 1 hit
SMARTiView protein in SMART
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVTI1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UEU0
Secondary accession number(s): O43547, Q96J28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 20, 2007
Last modified: September 12, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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