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Protein

Cathepsin F

Gene

CTSF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Catalytic activityi

The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (k(cat)/K(m)) comparable to that of cathepsin L.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2951 Publication1
Active sitei4311 Publication1
Active sitei451By similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.41 2681
ReactomeiR-HSA-2132295 MHC class II antigen presentation

Protein family/group databases

MEROPSiC01.018

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin F (EC:3.4.22.41)
Short name:
CATSF
Gene namesi
Name:CTSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000174080.10
HGNCiHGNC:2531 CTSF
MIMi603539 gene
neXtProtiNX_Q9UBX1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Ceroid lipofuscinosis, neuronal, 13 (CLN13)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of neuronal ceroid lipofuscinosis characterized by adult onset of progressive cognitive decline and motor dysfunction leading to dementia and often early death. Some patients develop seizures. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material.
See also OMIM:615362
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_070159231Y → C in CLN13. 1 PublicationCorresponds to variant dbSNP:rs143889283EnsemblClinVar.1
Natural variantiVAR_070160321Q → R in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514731EnsemblClinVar.1
Natural variantiVAR_070161458G → A in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514732EnsemblClinVar.1
Natural variantiVAR_070162480S → L in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514733EnsemblClinVar.1

Keywords - Diseasei

Disease mutation, Neurodegeneration, Neuronal ceroid lipofuscinosis

Organism-specific databases

DisGeNETi8722
GeneReviewsiCTSF
MalaCardsiCTSF
MIMi615362 phenotype
OpenTargetsiENSG00000174080
Orphaneti352709 CLN13 disease
PharmGKBiPA27031

Chemistry databases

ChEMBLiCHEMBL2517
DrugBankiDB02243 4-Morpholin-4-Yl-Piperidine-1-Carboxylic Acid [1-(3-Benzenesulfonyl-1-Propyl-Allylcarbamoyl)-2-Phenylethyl]-Amide
DB01871 [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester
DB03536 Benzoyl-Arginine-Alanine-Methyl Ketone
DB08775 BENZOYL-TYROSINE-ALANINE-METHYL KETONE
DB07913 HOMOPHENYLALANINYLMETHANE
DB03691 WRR-112
DB03573 WRR-99
GuidetoPHARMACOLOGYi2347

Polymorphism and mutation databases

BioMutaiCTSF
DMDMi12643325

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002620220 – 270Activation peptideAdd BLAST251
ChainiPRO_0000026203271 – 484Cathepsin FAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi195N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi292 ↔ 3331 Publication
Disulfide bondi326 ↔ 3661 Publication
Glycosylationi367N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi378N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi424 ↔ 472By similarity
Glycosylationi440N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ9UBX1
MaxQBiQ9UBX1
PaxDbiQ9UBX1
PeptideAtlasiQ9UBX1
PRIDEiQ9UBX1
ProteomicsDBi84089

PTM databases

GlyConnecti1080
iPTMnetiQ9UBX1
PhosphoSitePlusiQ9UBX1

Expressioni

Tissue specificityi

High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.

Gene expression databases

BgeeiENSG00000174080 Expressed in 233 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CTSF
ExpressionAtlasiQ9UBX1 baseline and differential
GenevisibleiQ9UBX1 HS

Organism-specific databases

HPAiCAB002141
HPA031431
HPA055610

Interactioni

Protein-protein interaction databases

BioGridi114261, 18 interactors
STRINGi9606.ENSP00000310832

Chemistry databases

BindingDBiQ9UBX1

Structurei

Secondary structure

1484
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UBX1
SMRiQ9UBX1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBX1

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1542 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiQ9UBX1
KOiK01373
OMAiSGIPFWA
OrthoDBiEOG091G05SD
PhylomeDBiQ9UBX1
TreeFamiTF314550

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9UBX1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPWLQLLSL LGLLPGAVAA PAQPRAASFQ AWGPPSPELL APTRFALEMF
60 70 80 90 100
NRGRAAGTRA VLGLVRGRVR RAGQGSLYSL EATLEEPPCN DPMVCRLPVS
110 120 130 140 150
KKTLLCSFQV LDELGRHVLL RKDCGPVDTK VPGAGEPKSA FTQGSAMISS
160 170 180 190 200
LSQNHPDNRN ETFSSVISLL NEDPLSQDLP VKMASIFKNF VITYNRTYES
210 220 230 240 250
KEEARWRLSV FVNNMVRAQK IQALDRGTAQ YGVTKFSDLT EEEFRTIYLN
260 270 280 290 300
TLLRKEPGNK MKQAKSVGDL APPEWDWRSK GAVTKVKDQG MCGSCWAFSV
310 320 330 340 350
TGNVEGQWFL NQGTLLSLSE QELLDCDKMD KACMGGLPSN AYSAIKNLGG
360 370 380 390 400
LETEDDYSYQ GHMQSCNFSA EKAKVYINDS VELSQNEQKL AAWLAKRGPI
410 420 430 440 450
SVAINAFGMQ FYRHGISRPL RPLCSPWLID HAVLLVGYGN RSDVPFWAIK
460 470 480
NSWGTDWGEK GYYYLHRGSG ACGVNTMASS AVVD
Length:484
Mass (Da):53,366
Last modified:May 1, 2000 - v1
Checksum:i1D5D551B489D822B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YD65H0YD65_HUMAN
Cathepsin F
CTSF
282Annotation score:
H0YE42H0YE42_HUMAN
Cathepsin F
CTSF
82Annotation score:
E9PSC2E9PSC2_HUMAN
Cathepsin F
CTSF
120Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti305E → K in AAF13146 (Ref. 5) Curated1
Sequence conflicti442 – 484SDVPF…SAVVD → EFRCLSCIQPGHRQGWDHSI SGPLEGK (PubMed:17974005).CuratedAdd BLAST43

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051513153Q → R. Corresponds to variant dbSNP:rs11550508EnsemblClinVar.1
Natural variantiVAR_070159231Y → C in CLN13. 1 PublicationCorresponds to variant dbSNP:rs143889283EnsemblClinVar.1
Natural variantiVAR_070160321Q → R in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514731EnsemblClinVar.1
Natural variantiVAR_070161458G → A in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514732EnsemblClinVar.1
Natural variantiVAR_070162480S → L in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514733EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007331 mRNA Translation: CAB42883.1
AF088886 mRNA Translation: AAD26616.2
AF132894 Genomic DNA Translation: AAD41790.1
AF136279 mRNA Translation: AAF13146.1
AF071748 mRNA Translation: AAC78838.1
AF071749 mRNA Translation: AAC78839.1
AK313657 mRNA Translation: BAG36411.1
BC011682 mRNA Translation: AAH11682.1
BC036451 mRNA Translation: AAH36451.1
AL137742 mRNA Translation: CAB70900.1
CCDSiCCDS8144.1
RefSeqiNP_003784.2, NM_003793.3
UniGeneiHs.11590

Genome annotation databases

EnsembliENST00000310325; ENSP00000310832; ENSG00000174080
GeneIDi8722
KEGGihsa:8722
UCSCiuc001oip.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007331 mRNA Translation: CAB42883.1
AF088886 mRNA Translation: AAD26616.2
AF132894 Genomic DNA Translation: AAD41790.1
AF136279 mRNA Translation: AAF13146.1
AF071748 mRNA Translation: AAC78838.1
AF071749 mRNA Translation: AAC78839.1
AK313657 mRNA Translation: BAG36411.1
BC011682 mRNA Translation: AAH11682.1
BC036451 mRNA Translation: AAH36451.1
AL137742 mRNA Translation: CAB70900.1
CCDSiCCDS8144.1
RefSeqiNP_003784.2, NM_003793.3
UniGeneiHs.11590

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5Umodel-A271-484[»]
1M6DX-ray1.70A/B271-484[»]
ProteinModelPortaliQ9UBX1
SMRiQ9UBX1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114261, 18 interactors
STRINGi9606.ENSP00000310832

Chemistry databases

BindingDBiQ9UBX1
ChEMBLiCHEMBL2517
DrugBankiDB02243 4-Morpholin-4-Yl-Piperidine-1-Carboxylic Acid [1-(3-Benzenesulfonyl-1-Propyl-Allylcarbamoyl)-2-Phenylethyl]-Amide
DB01871 [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester
DB03536 Benzoyl-Arginine-Alanine-Methyl Ketone
DB08775 BENZOYL-TYROSINE-ALANINE-METHYL KETONE
DB07913 HOMOPHENYLALANINYLMETHANE
DB03691 WRR-112
DB03573 WRR-99
GuidetoPHARMACOLOGYi2347

Protein family/group databases

MEROPSiC01.018

PTM databases

GlyConnecti1080
iPTMnetiQ9UBX1
PhosphoSitePlusiQ9UBX1

Polymorphism and mutation databases

BioMutaiCTSF
DMDMi12643325

Proteomic databases

EPDiQ9UBX1
MaxQBiQ9UBX1
PaxDbiQ9UBX1
PeptideAtlasiQ9UBX1
PRIDEiQ9UBX1
ProteomicsDBi84089

Protocols and materials databases

DNASUi8722
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310325; ENSP00000310832; ENSG00000174080
GeneIDi8722
KEGGihsa:8722
UCSCiuc001oip.4 human

Organism-specific databases

CTDi8722
DisGeNETi8722
EuPathDBiHostDB:ENSG00000174080.10
GeneCardsiCTSF
GeneReviewsiCTSF
HGNCiHGNC:2531 CTSF
HPAiCAB002141
HPA031431
HPA055610
MalaCardsiCTSF
MIMi603539 gene
615362 phenotype
neXtProtiNX_Q9UBX1
OpenTargetsiENSG00000174080
Orphaneti352709 CLN13 disease
PharmGKBiPA27031
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1542 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiQ9UBX1
KOiK01373
OMAiSGIPFWA
OrthoDBiEOG091G05SD
PhylomeDBiQ9UBX1
TreeFamiTF314550

Enzyme and pathway databases

BRENDAi3.4.22.41 2681
ReactomeiR-HSA-2132295 MHC class II antigen presentation

Miscellaneous databases

ChiTaRSiCTSF human
EvolutionaryTraceiQ9UBX1
GeneWikiiCathepsin_F
GenomeRNAii8722
PROiPR:Q9UBX1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174080 Expressed in 233 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_CTSF
ExpressionAtlasiQ9UBX1 baseline and differential
GenevisibleiQ9UBX1 HS

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCATF_HUMAN
AccessioniPrimary (citable) accession number: Q9UBX1
Secondary accession number(s): B2R964
, O95240, Q9NSU4, Q9UKQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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