UniProtKB - Q9UBX1 (CATF_HUMAN)
Protein
Cathepsin F
Gene
CTSF
Organism
Homo sapiens (Human)
Status
Functioni
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Catalytic activityi
- The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (k(cat)/K(m)) comparable to that of cathepsin L. EC:3.4.22.41
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 295 | 1 Publication | 1 | |
Active sitei | 431 | 1 Publication | 1 | |
Active sitei | 451 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- proteolysis Source: ProtInc
- proteolysis involved in cellular protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.41, 2681 |
PathwayCommonsi | Q9UBX1 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation |
Protein family/group databases
MEROPSi | C01.018 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CTSF |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2531, CTSF |
MIMi | 603539, gene |
neXtProti | NX_Q9UBX1 |
VEuPathDBi | HostDB:ENSG00000174080.10 |
Subcellular locationi
Lysosome
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular exosome Source: UniProtKB
- extracellular space Source: GO_Central
- extracellular vesicle Source: UniProtKB
Lysosome
- lysosomal lumen Source: Reactome
- lysosome Source: GO_Central
Keywords - Cellular componenti
LysosomePathology & Biotechi
Involvement in diseasei
Ceroid lipofuscinosis, neuronal, 13 (CLN13)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of neuronal ceroid lipofuscinosis characterized by adult onset of progressive cognitive decline and motor dysfunction leading to dementia and often early death. Some patients develop seizures. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_070159 | 231 | Y → C in CLN13. 1 PublicationCorresponds to variant dbSNP:rs143889283EnsemblClinVar. | 1 | |
Natural variantiVAR_070160 | 321 | Q → R in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514731Ensembl. | 1 | |
Natural variantiVAR_070161 | 458 | G → A in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514732EnsemblClinVar. | 1 | |
Natural variantiVAR_070162 | 480 | S → L in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514733EnsemblClinVar. | 1 |
Keywords - Diseasei
Disease variant, Neurodegeneration, Neuronal ceroid lipofuscinosisOrganism-specific databases
DisGeNETi | 8722 |
GeneReviewsi | CTSF |
MalaCardsi | CTSF |
MIMi | 615362, phenotype |
OpenTargetsi | ENSG00000174080 |
Orphaneti | 352709, CLN13 disease |
PharmGKBi | PA27031 |
Miscellaneous databases
Pharosi | Q9UBX1, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2517 |
DrugBanki | DB02243, 4-Morpholin-4-Yl-Piperidine-1-Carboxylic Acid [1-(3-Benzenesulfonyl-1-Propyl-Allylcarbamoyl)-2-Phenylethyl]-Amide DB01871, [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester DB01810, [1-(1-Methyl-4,5-Dioxo-Pent-2-Enylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester DB08775, Benzoyl-tyrosine-alanine-fluoro-methyl ketone DB03536, Benzyl N-[(2S)-5-(diaminomethylamino)-1-[[(2S)-4-fluoro-3-oxobutan-2-yl]amino]-1-oxopentan-2-yl]carbamate DB07913, HOMOPHENYLALANINYLMETHANE DB03691, WRR-112 DB03573, WRR-99 |
DrugCentrali | Q9UBX1 |
GuidetoPHARMACOLOGYi | 2347 |
Genetic variation databases
BioMutai | CTSF |
DMDMi | 12643325 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000026202 | 20 – 270 | Activation peptideAdd BLAST | 251 | |
ChainiPRO_0000026203 | 271 – 484 | Cathepsin FAdd BLAST | 214 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 160 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 195 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 292 ↔ 333 | 1 Publication | ||
Disulfide bondi | 326 ↔ 366 | 1 Publication | ||
Glycosylationi | 367 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 378 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 424 ↔ 472 | By similarity | ||
Glycosylationi | 440 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
EPDi | Q9UBX1 |
jPOSTi | Q9UBX1 |
MassIVEi | Q9UBX1 |
MaxQBi | Q9UBX1 |
PaxDbi | Q9UBX1 |
PeptideAtlasi | Q9UBX1 |
PRIDEi | Q9UBX1 |
ProteomicsDBi | 84089 |
PTM databases
GlyConnecti | 1080, 4 N-Linked glycans (2 sites) |
GlyGeni | Q9UBX1, 5 sites |
iPTMneti | Q9UBX1 |
PhosphoSitePlusi | Q9UBX1 |
Expressioni
Tissue specificityi
High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
Gene expression databases
Bgeei | ENSG00000174080, Expressed in right hemisphere of cerebellum and 247 other tissues |
ExpressionAtlasi | Q9UBX1, baseline and differential |
Genevisiblei | Q9UBX1, HS |
Organism-specific databases
HPAi | ENSG00000174080, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114261, 19 interactors |
IntActi | Q9UBX1, 8 interactors |
STRINGi | 9606.ENSP00000310832 |
Chemistry databases
BindingDBi | Q9UBX1 |
Miscellaneous databases
RNActi | Q9UBX1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UBX1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UBX1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1542, Eukaryota |
GeneTreei | ENSGT00940000162141 |
HOGENOMi | CLU_012184_10_0_1 |
InParanoidi | Q9UBX1 |
OMAi | RGHMQAC |
OrthoDBi | 1264766at2759 |
PhylomeDBi | Q9UBX1 |
TreeFami | TF314550 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9UBX1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPWLQLLSL LGLLPGAVAA PAQPRAASFQ AWGPPSPELL APTRFALEMF
60 70 80 90 100
NRGRAAGTRA VLGLVRGRVR RAGQGSLYSL EATLEEPPCN DPMVCRLPVS
110 120 130 140 150
KKTLLCSFQV LDELGRHVLL RKDCGPVDTK VPGAGEPKSA FTQGSAMISS
160 170 180 190 200
LSQNHPDNRN ETFSSVISLL NEDPLSQDLP VKMASIFKNF VITYNRTYES
210 220 230 240 250
KEEARWRLSV FVNNMVRAQK IQALDRGTAQ YGVTKFSDLT EEEFRTIYLN
260 270 280 290 300
TLLRKEPGNK MKQAKSVGDL APPEWDWRSK GAVTKVKDQG MCGSCWAFSV
310 320 330 340 350
TGNVEGQWFL NQGTLLSLSE QELLDCDKMD KACMGGLPSN AYSAIKNLGG
360 370 380 390 400
LETEDDYSYQ GHMQSCNFSA EKAKVYINDS VELSQNEQKL AAWLAKRGPI
410 420 430 440 450
SVAINAFGMQ FYRHGISRPL RPLCSPWLID HAVLLVGYGN RSDVPFWAIK
460 470 480
NSWGTDWGEK GYYYLHRGSG ACGVNTMASS AVVD
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YD65 | H0YD65_HUMAN | Cathepsin F | CTSF | 282 | Annotation score: | ||
H0YE42 | H0YE42_HUMAN | Cathepsin F | CTSF | 82 | Annotation score: | ||
E9PSC2 | E9PSC2_HUMAN | Cathepsin F | CTSF | 120 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 305 | E → K in AAF13146 (Ref. 5) Curated | 1 | |
Sequence conflicti | 442 – 484 | SDVPF…SAVVD → EFRCLSCIQPGHRQGWDHSI SGPLEGK (PubMed:17974005).CuratedAdd BLAST | 43 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051513 | 153 | Q → R. Corresponds to variant dbSNP:rs11550508EnsemblClinVar. | 1 | |
Natural variantiVAR_070159 | 231 | Y → C in CLN13. 1 PublicationCorresponds to variant dbSNP:rs143889283EnsemblClinVar. | 1 | |
Natural variantiVAR_070160 | 321 | Q → R in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514731Ensembl. | 1 | |
Natural variantiVAR_070161 | 458 | G → A in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514732EnsemblClinVar. | 1 | |
Natural variantiVAR_070162 | 480 | S → L in CLN13. 1 PublicationCorresponds to variant dbSNP:rs397514733EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ007331 mRNA Translation: CAB42883.1 AF088886 mRNA Translation: AAD26616.2 AF132894 Genomic DNA Translation: AAD41790.1 AF136279 mRNA Translation: AAF13146.1 AF071748 mRNA Translation: AAC78838.1 AF071749 mRNA Translation: AAC78839.1 AK313657 mRNA Translation: BAG36411.1 BC011682 mRNA Translation: AAH11682.1 BC036451 mRNA Translation: AAH36451.1 AL137742 mRNA Translation: CAB70900.1 |
CCDSi | CCDS8144.1 |
RefSeqi | NP_003784.2, NM_003793.3 |
Genome annotation databases
Ensembli | ENST00000310325; ENSP00000310832; ENSG00000174080 |
GeneIDi | 8722 |
KEGGi | hsa:8722 |
UCSCi | uc001oip.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ007331 mRNA Translation: CAB42883.1 AF088886 mRNA Translation: AAD26616.2 AF132894 Genomic DNA Translation: AAD41790.1 AF136279 mRNA Translation: AAF13146.1 AF071748 mRNA Translation: AAC78838.1 AF071749 mRNA Translation: AAC78839.1 AK313657 mRNA Translation: BAG36411.1 BC011682 mRNA Translation: AAH11682.1 BC036451 mRNA Translation: AAH36451.1 AL137742 mRNA Translation: CAB70900.1 |
CCDSi | CCDS8144.1 |
RefSeqi | NP_003784.2, NM_003793.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D5U | model | - | A | 271-484 | [»] | |
1M6D | X-ray | 1.70 | A/B | 271-484 | [»] | |
SMRi | Q9UBX1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114261, 19 interactors |
IntActi | Q9UBX1, 8 interactors |
STRINGi | 9606.ENSP00000310832 |
Chemistry databases
BindingDBi | Q9UBX1 |
ChEMBLi | CHEMBL2517 |
DrugBanki | DB02243, 4-Morpholin-4-Yl-Piperidine-1-Carboxylic Acid [1-(3-Benzenesulfonyl-1-Propyl-Allylcarbamoyl)-2-Phenylethyl]-Amide DB01871, [1-(1-Benzyl-3-Hydroxy-2-Oxo-Propylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester DB01810, [1-(1-Methyl-4,5-Dioxo-Pent-2-Enylcarbamoyl)-2-Phenyl-Ethyl]-Carbamic Acid Benzyl Ester DB08775, Benzoyl-tyrosine-alanine-fluoro-methyl ketone DB03536, Benzyl N-[(2S)-5-(diaminomethylamino)-1-[[(2S)-4-fluoro-3-oxobutan-2-yl]amino]-1-oxopentan-2-yl]carbamate DB07913, HOMOPHENYLALANINYLMETHANE DB03691, WRR-112 DB03573, WRR-99 |
DrugCentrali | Q9UBX1 |
GuidetoPHARMACOLOGYi | 2347 |
Protein family/group databases
MEROPSi | C01.018 |
PTM databases
GlyConnecti | 1080, 4 N-Linked glycans (2 sites) |
GlyGeni | Q9UBX1, 5 sites |
iPTMneti | Q9UBX1 |
PhosphoSitePlusi | Q9UBX1 |
Genetic variation databases
BioMutai | CTSF |
DMDMi | 12643325 |
Proteomic databases
EPDi | Q9UBX1 |
jPOSTi | Q9UBX1 |
MassIVEi | Q9UBX1 |
MaxQBi | Q9UBX1 |
PaxDbi | Q9UBX1 |
PeptideAtlasi | Q9UBX1 |
PRIDEi | Q9UBX1 |
ProteomicsDBi | 84089 |
Protocols and materials databases
Antibodypediai | 30202, 273 antibodies |
DNASUi | 8722 |
Genome annotation databases
Ensembli | ENST00000310325; ENSP00000310832; ENSG00000174080 |
GeneIDi | 8722 |
KEGGi | hsa:8722 |
UCSCi | uc001oip.4, human |
Organism-specific databases
CTDi | 8722 |
DisGeNETi | 8722 |
GeneCardsi | CTSF |
GeneReviewsi | CTSF |
HGNCi | HGNC:2531, CTSF |
HPAi | ENSG00000174080, Low tissue specificity |
MalaCardsi | CTSF |
MIMi | 603539, gene 615362, phenotype |
neXtProti | NX_Q9UBX1 |
OpenTargetsi | ENSG00000174080 |
Orphaneti | 352709, CLN13 disease |
PharmGKBi | PA27031 |
VEuPathDBi | HostDB:ENSG00000174080.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1542, Eukaryota |
GeneTreei | ENSGT00940000162141 |
HOGENOMi | CLU_012184_10_0_1 |
InParanoidi | Q9UBX1 |
OMAi | RGHMQAC |
OrthoDBi | 1264766at2759 |
PhylomeDBi | Q9UBX1 |
TreeFami | TF314550 |
Enzyme and pathway databases
BRENDAi | 3.4.22.41, 2681 |
PathwayCommonsi | Q9UBX1 |
Reactomei | R-HSA-2132295, MHC class II antigen presentation |
Miscellaneous databases
BioGRID-ORCSi | 8722, 19 hits in 874 CRISPR screens |
ChiTaRSi | CTSF, human |
EvolutionaryTracei | Q9UBX1 |
GeneWikii | Cathepsin_F |
GenomeRNAii | 8722 |
Pharosi | Q9UBX1, Tchem |
PROi | PR:Q9UBX1 |
RNActi | Q9UBX1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174080, Expressed in right hemisphere of cerebellum and 247 other tissues |
ExpressionAtlasi | Q9UBX1, baseline and differential |
Genevisiblei | Q9UBX1, HS |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CATF_HUMAN | |
Accessioni | Q9UBX1Primary (citable) accession number: Q9UBX1 Secondary accession number(s): B2R964 Q9UKQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families