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Protein

Bridging integrator 2

Gene

BIN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.1 Publication

GO - Molecular functioni

  • phospholipid binding Source: UniProtKB

GO - Biological processi

  • cell chemotaxis Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • phagocytosis, engulfment Source: UniProtKB
  • plasma membrane tubulation Source: UniProtKB
  • podosome assembly Source: UniProtKB

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bridging integrator 2
Alternative name(s):
Breast cancer-associated protein 1
Gene namesi
Name:BIN2
Synonyms:BRAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000110934.10
HGNCiHGNC:1053 BIN2
MIMi605936 gene
neXtProtiNX_Q9UBW5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13F → A: Strongly reduces binding to membranes. 1 Publication1
Mutagenesisi21F → A: Strongly reduces binding to membranes. 1 Publication1
Mutagenesisi81V → R: Abolishes dimerization and membrane binding; when associated with E-214. 1 Publication1
Mutagenesisi214S → E: Abolishes dimerization and membrane binding; when associated with R-81. 1 Publication1

Organism-specific databases

DisGeNETi51411
OpenTargetsiENSG00000110934
PharmGKBiPA25356

Polymorphism and mutation databases

BioMutaiBIN2
DMDMi143811367

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002561401 – 565Bridging integrator 2Add BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273PhosphoserineCombined sources1
Modified residuei357PhosphoserineCombined sources1
Modified residuei383PhosphoserineBy similarity1
Modified residuei395PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei438PhosphothreonineCombined sources1
Modified residuei440PhosphoserineBy similarity1
Modified residuei444PhosphoserineCombined sources1
Modified residuei447PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1
Modified residuei462PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UBW5
MaxQBiF5H0W4
PaxDbiQ9UBW5
PeptideAtlasiQ9UBW5
PRIDEiQ9UBW5
ProteomicsDBi84083
84084 [Q9UBW5-2]

2D gel databases

OGPiQ9UBW5

PTM databases

iPTMnetiQ9UBW5
PhosphoSitePlusiQ9UBW5
SwissPalmiQ9UBW5

Expressioni

Tissue specificityi

Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues.2 Publications

Inductioni

Up-regulated during monocytic differentiation.1 Publication

Gene expression databases

BgeeiENSG00000110934 Expressed in 140 organ(s), highest expression level in blood
CleanExiHS_BIN2
ExpressionAtlasiF5H0W4 baseline and differential

Organism-specific databases

HPAiHPA038666
HPA038667

Interactioni

Subunit structurei

Homodimer. Interacts with BIN1. Interacts with ARHGEF6 (via SH3 domain), ARHGEF7 (via SH3 domain), SH3GL1, SH3GL2 and SH3GL3. Identified in a complex with ARHGEF6 and GIT2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BIN1O004992EBI-2042570,EBI-719094

Protein-protein interaction databases

BioGridi119528, 7 interactors
IntActiQ9UBW5, 7 interactors
STRINGi9606.ENSP00000267012

Structurei

Secondary structure

1565
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UBW5
SMRiQ9UBW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 244BARPROSITE-ProRule annotationAdd BLAST217

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili304 – 329Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi253 – 256Poly-Ser4
Compositional biasi413 – 479Pro-richAdd BLAST67

Domaini

The BAR domain mediates dimerization and interaction with membranes enriched in phosphatidylinositides.1 Publication

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ITR5 Eukaryota
ENOG410Y56Y LUCA
GeneTreeiENSGT00390000017588
HOGENOMiHOG000252987
HOVERGENiHBG004224
InParanoidiQ9UBW5
KOiK20119
PhylomeDBiQ9UBW5
TreeFamiTF313542

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR003005 Amphiphysin
IPR004148 BAR_dom
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PRINTSiPR01251 AMPHIPHYSIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q9UBW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEGKAGGAA GLFAKQVQKK FSRAQEKVLQ KLGKAVETKD ERFEQSASNF
60 70 80 90 100
YQQQAEGHKL YKDLKNFLSA VKVMHESSKR VSETLQEIYS SEWDGHEELK
110 120 130 140 150
AIVWNNDLLW EDYEEKLADQ AVRTMEIYVA QFSEIKERIA KRGRKLVDYD
160 170 180 190 200
SARHHLEAVQ NAKKKDEAKT AKAEEEFNKA QTVFEDLNQE LLEELPILYN
210 220 230 240 250
SRIGCYVTIF QNISNLRDVF YREMSKLNHN LYEVMSKLEK QHSNKVFVVK
260 270 280 290 300
GLSSSSRRSL VISPPVRTAT VSSPLTSPTS PSTLSLKSES ESVSATEDLA
310 320 330 340 350
PDAAQGEDNS EIKELLEEEE IEKEGSEASS SEEDEPLPAC NGPAQAQPSP
360 370 380 390 400
TTERAKSQEE VLPSSTTPSP GGALSPSGQP SSSATEVVLR TRTASEGSEQ
410 420 430 440 450
PKKRASIQRT SAPPSRPPPP RATASPRPSS GNIPSSPTAS GGGSPTSPRA
460 470 480 490 500
SLGTGTASPR TSLEVSPNPE PPEKPVRTPE AKENENIHNQ NPEELCTSPT
510 520 530 540 550
LMTSQVASEP GEAKKMEDKE KDNKLISANS SEGQDQLQVS MVPENNNLTA
560
PEPQEEVSTS ENPQL
Length:565
Mass (Da):61,874
Last modified:April 3, 2007 - v3
Checksum:iB1D1FB89A97E9E43
GO
Isoform 2 (identifier: Q9UBW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-136: Missing.

Note: No experimental confirmation available.
Show »
Length:533
Mass (Da):58,030
Checksum:iE549F6F418F01E02
GO
Isoform 3 (identifier: Q9UBW5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MAEGKAGGAAGLFAKQVQKKFSRAQEK → M

Note: No experimental confirmation available.Curated
Show »
Length:539
Mass (Da):59,186
Checksum:i062E5E8E5ED86C96
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087X188A0A087X188_HUMAN
Bridging integrator 2
BIN2
597Annotation score:
S4R418S4R418_HUMAN
Bridging integrator 2
BIN2
536Annotation score:
A0A0A0MT10A0A0A0MT10_HUMAN
Bridging integrator 2
BIN2
551Annotation score:
S4R3J0S4R3J0_HUMAN
Bridging integrator 2
BIN2
73Annotation score:

Sequence cautioni

The sequence BAA91376 differs from that shown. Reason: Frameshift at position 156.Curated
The sequence BAA91376 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163Missing in AAD54227 (PubMed:10903846).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02888348S → N1 PublicationCorresponds to variant dbSNP:rs7312857Ensembl.1
Natural variantiVAR_028884529N → D4 PublicationsCorresponds to variant dbSNP:rs7954976Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0597311 – 27MAEGK…RAQEK → M in isoform 3. Add BLAST27
Alternative sequenceiVSP_021328105 – 136Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146531 mRNA Translation: AAD54227.1
AB032698 mRNA Translation: BAA88108.1
AB032710 Genomic DNA Translation: BAA88125.1
AK000783 mRNA Translation: BAA91376.1 Sequence problems.
AK300211 mRNA Translation: BAH13239.1
AC046135 Genomic DNA No translation available.
BC047686 mRNA Translation: AAH47686.1
RefSeqiNP_001276936.1, NM_001290007.1
NP_001276937.1, NM_001290008.1
NP_001276938.1, NM_001290009.1
NP_057377.3, NM_016293.3
UniGeneiHs.14770

Genome annotation databases

EnsembliENST00000267012; ENSP00000267012; ENSG00000110934
GeneIDi51411
KEGGihsa:51411
UCSCiuc058ocp.1 human [Q9UBW5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146531 mRNA Translation: AAD54227.1
AB032698 mRNA Translation: BAA88108.1
AB032710 Genomic DNA Translation: BAA88125.1
AK000783 mRNA Translation: BAA91376.1 Sequence problems.
AK300211 mRNA Translation: BAH13239.1
AC046135 Genomic DNA No translation available.
BC047686 mRNA Translation: AAH47686.1
RefSeqiNP_001276936.1, NM_001290007.1
NP_001276937.1, NM_001290008.1
NP_001276938.1, NM_001290009.1
NP_057377.3, NM_016293.3
UniGeneiHs.14770

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AVMX-ray1.91A11-245[»]
4I1QX-ray2.53A/B20-238[»]
ProteinModelPortaliQ9UBW5
SMRiQ9UBW5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119528, 7 interactors
IntActiQ9UBW5, 7 interactors
STRINGi9606.ENSP00000267012

PTM databases

iPTMnetiQ9UBW5
PhosphoSitePlusiQ9UBW5
SwissPalmiQ9UBW5

Polymorphism and mutation databases

BioMutaiBIN2
DMDMi143811367

2D gel databases

OGPiQ9UBW5

Proteomic databases

EPDiQ9UBW5
MaxQBiF5H0W4
PaxDbiQ9UBW5
PeptideAtlasiQ9UBW5
PRIDEiQ9UBW5
ProteomicsDBi84083
84084 [Q9UBW5-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267012; ENSP00000267012; ENSG00000110934
GeneIDi51411
KEGGihsa:51411
UCSCiuc058ocp.1 human [Q9UBW5-1]

Organism-specific databases

CTDi51411
DisGeNETi51411
EuPathDBiHostDB:ENSG00000110934.10
GeneCardsiBIN2
H-InvDBiHIX0201823
HGNCiHGNC:1053 BIN2
HPAiHPA038666
HPA038667
MIMi605936 gene
neXtProtiNX_Q9UBW5
OpenTargetsiENSG00000110934
PharmGKBiPA25356
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITR5 Eukaryota
ENOG410Y56Y LUCA
GeneTreeiENSGT00390000017588
HOGENOMiHOG000252987
HOVERGENiHBG004224
InParanoidiQ9UBW5
KOiK20119
PhylomeDBiQ9UBW5
TreeFamiTF313542

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiBIN2 human
GenomeRNAii51411
PROiPR:Q9UBW5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110934 Expressed in 140 organ(s), highest expression level in blood
CleanExiHS_BIN2
ExpressionAtlasiF5H0W4 baseline and differential

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR003005 Amphiphysin
IPR004148 BAR_dom
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PRINTSiPR01251 AMPHIPHYSIN
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIN2_HUMAN
AccessioniPrimary (citable) accession number: Q9UBW5
Secondary accession number(s): B7Z6F3
, F5H0W4, Q86VV0, Q9NWK4, Q9UKN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 3, 2007
Last modified: September 12, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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