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Protein

E3 ubiquitin-protein ligase RNF14

Gene

RNF14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.1 Publication

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi220Zinc 1PROSITE-ProRule annotation1
Metal bindingi223Zinc 1PROSITE-ProRule annotation1
Metal bindingi238Zinc 2PROSITE-ProRule annotation1
Metal bindingi240Zinc 2; via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi243Zinc 1PROSITE-ProRule annotation1
Metal bindingi246Zinc 1PROSITE-ProRule annotation1
Metal bindingi265Zinc 2PROSITE-ProRule annotation1
Metal bindingi270Zinc 2PROSITE-ProRule annotation1
Metal bindingi309Zinc 3PROSITE-ProRule annotation1
Metal bindingi314Zinc 3PROSITE-ProRule annotation1
Metal bindingi329Zinc 3PROSITE-ProRule annotation1
Metal bindingi332Zinc 3PROSITE-ProRule annotation1
Metal bindingi337Zinc 4PROSITE-ProRule annotation1
Metal bindingi340Zinc 4PROSITE-ProRule annotation1
Metal bindingi345Zinc 4; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi350Zinc 4PROSITE-ProRule annotation1
Metal bindingi404Zinc 5PROSITE-ProRule annotation1
Metal bindingi407Zinc 5PROSITE-ProRule annotation1
Active sitei417PROSITE-ProRule annotation1
Metal bindingi422Zinc 5PROSITE-ProRule annotation1
Metal bindingi425Zinc 5PROSITE-ProRule annotation1
Metal bindingi430Zinc 6PROSITE-ProRule annotation1
Metal bindingi433Zinc 6PROSITE-ProRule annotation1
Metal bindingi445Zinc 6; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi453Zinc 6PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri220 – 270RING-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri289 – 350IBR-typePROSITE-ProRule annotationAdd BLAST62
Zinc fingeri404 – 433RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • transcription coactivator activity Source: UniProtKB
  • ubiquitin conjugating enzyme binding Source: GO_Central
  • ubiquitin-like protein transferase activity Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF14 (EC:2.3.2.31By similarity)
Alternative name(s):
Androgen receptor-associated protein 54
HFB30
RING finger protein 14
Triad2 protein
Gene namesi
Name:RNF14
Synonyms:ARA54
ORF Names:HRIHFB2038
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000013561.17
HGNCiHGNC:10058 RNF14
MIMi605675 gene
neXtProtiNX_Q9UBS8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi220C → S: Loss of interaction with UBE2E2 and of autoubiquitination. 1 Publication1

Organism-specific databases

DisGeNETi9604
OpenTargetsiENSG00000013561
PharmGKBiPA34423

Polymorphism and mutation databases

BioMutaiRNF14
DMDMi17380293

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560571 – 474E3 ubiquitin-protein ligase RNF14Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei348PhosphoserineCombined sources1

Post-translational modificationi

RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UBS8
PaxDbiQ9UBS8
PeptideAtlasiQ9UBS8
PRIDEiQ9UBS8
ProteomicsDBi84052

PTM databases

iPTMnetiQ9UBS8
PhosphoSitePlusiQ9UBS8

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSG00000013561 Expressed in 227 organ(s), highest expression level in testis
CleanExiHS_RNF14
ExpressionAtlasiQ9UBS8 baseline and differential
GenevisibleiQ9UBS8 HS

Organism-specific databases

HPAiHPA008716

Interactioni

Subunit structurei

Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114968, 30 interactors
IntActiQ9UBS8, 18 interactors
MINTiQ9UBS8
STRINGi9606.ENSP00000324956

Structurei

3D structure databases

ProteinModelPortaliQ9UBS8
SMRiQ9UBS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 137RWDPROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 457TRIAD supradomainPROSITE-ProRule annotationAdd BLAST242
Regioni361 – 474Interaction with androgen receptorAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili351 – 395Sequence analysisAdd BLAST45

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45D-box9

Domaini

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The RING-type zinc finger is essential for the interaction with UBE2E2.
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF14 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri220 – 270RING-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri289 – 350IBR-typePROSITE-ProRule annotationAdd BLAST62
Zinc fingeri404 – 433RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1814 Eukaryota
ENOG410Y5R1 LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000237334
HOVERGENiHBG057296
InParanoidiQ9UBS8
KOiK11971
OMAiGDIWEDE
OrthoDBiEOG091G08QR
PhylomeDBiQ9UBS8
TreeFamiTF314401

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS51873 TRIAD, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UBS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSEDREAQE DELLALASIY DGDEFRKAES VQGGETRIYL DLPQNFKIFV
60 70 80 90 100
SGNSNECLQN SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP
110 120 130 140 150
TQLSALCKHL DNLWEEHRGS VVLFAWMQFL KEETLAYLNI VSPFELKIGS
160 170 180 190 200
QKKVQRRTAQ ASPNTELDFG GAAGSDVDQE EIVDERAVQD VESLSNLIQE
210 220 230 240 250
ILDFDQAQQI KCFNSKLFLC SICFCEKLGS ECMYFLECRH VYCKACLKDY
260 270 280 290 300
FEIQIRDGQV QCLNCPEPKC PSVATPGQVK ELVEAELFAR YDRLLLQSSL
310 320 330 340 350
DLMADVVYCP RPCCQLPVMQ EPGCTMGICS SCNFAFCTLC RLTYHGVSPC
360 370 380 390 400
KVTAEKLMDL RNEYLQADEA NKRLLDQRYG KRVIQKALEE MESKEWLEKN
410 420 430 440 450
SKSCPCCGTP IEKLDGCNKM TCTGCMQYFC WICMGSLSRA NPYKHFNDPG
460 470
SPCFNRLFYA VDVDDDIWED EVED
Length:474
Mass (Da):53,837
Last modified:May 1, 2000 - v1
Checksum:i529E3F5AF38A5DAD
GO
Isoform 2 (identifier: Q9UBS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.

Note: No experimental confirmation available.
Show »
Length:348
Mass (Da):39,634
Checksum:iC43770874596DD0C
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EP22E7EP22_HUMAN
RBR-type E3 ubiquitin transferase
RNF14
152Annotation score:
D6RBU3D6RBU3_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
185Annotation score:
D6R996D6R996_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
132Annotation score:
D6RA38D6RA38_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
278Annotation score:
D6RDU6D6RDU6_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
171Annotation score:
D6RAS4D6RAS4_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
99Annotation score:
D6R988D6R988_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
61Annotation score:
D6RBK4D6RBK4_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
64Annotation score:
D6RDK2D6RDK2_HUMAN
E3 ubiquitin-protein ligase RNF14
RNF14
15Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32Q → R in CAG32983 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0457801 – 126Missing in isoform 2. 1 PublicationAdd BLAST126

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022663 mRNA Translation: BAA78677.1
AF060544 mRNA Translation: AAD21842.1
AK023884 mRNA Translation: BAG51234.1
AK057868 mRNA No translation available.
CR456702 mRNA Translation: CAG32983.1
AC005740 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW61895.1
CH471062 Genomic DNA Translation: EAW61896.1
CH471062 Genomic DNA Translation: EAW61897.1
BC126185 mRNA Translation: AAI26186.1
BC144061 mRNA Translation: AAI44062.1
AB015333 mRNA Translation: BAA34792.1
CCDSiCCDS4270.1 [Q9UBS8-1]
CCDS4271.1 [Q9UBS8-2]
RefSeqiNP_001188294.1, NM_001201365.1 [Q9UBS8-1]
NP_004281.1, NM_004290.4 [Q9UBS8-1]
NP_899645.1, NM_183398.2 [Q9UBS8-2]
NP_899646.1, NM_183399.2 [Q9UBS8-1]
NP_899647.1, NM_183400.2 [Q9UBS8-1]
NP_899648.1, NM_183401.2 [Q9UBS8-1]
XP_005268593.1, XM_005268536.2
XP_005268594.1, XM_005268537.2
XP_005268596.1, XM_005268539.2 [Q9UBS8-2]
XP_005268597.1, XM_005268540.3 [Q9UBS8-2]
XP_005268598.1, XM_005268541.3 [Q9UBS8-2]
XP_011536016.1, XM_011537714.2 [Q9UBS8-1]
XP_016865559.1, XM_017010070.1
XP_016865560.1, XM_017010071.1 [Q9UBS8-2]
XP_016865561.1, XM_017010072.1 [Q9UBS8-2]
UniGeneiHs.483616

Genome annotation databases

EnsembliENST00000347642; ENSP00000324956; ENSG00000013561 [Q9UBS8-1]
ENST00000356143; ENSP00000348462; ENSG00000013561 [Q9UBS8-1]
ENST00000394514; ENSP00000378022; ENSG00000013561 [Q9UBS8-2]
ENST00000394519; ENSP00000378027; ENSG00000013561 [Q9UBS8-1]
ENST00000394520; ENSP00000378028; ENSG00000013561 [Q9UBS8-1]
GeneIDi9604
KEGGihsa:9604
UCSCiuc003lly.4 human [Q9UBS8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022663 mRNA Translation: BAA78677.1
AF060544 mRNA Translation: AAD21842.1
AK023884 mRNA Translation: BAG51234.1
AK057868 mRNA No translation available.
CR456702 mRNA Translation: CAG32983.1
AC005740 Genomic DNA No translation available.
CH471062 Genomic DNA Translation: EAW61895.1
CH471062 Genomic DNA Translation: EAW61896.1
CH471062 Genomic DNA Translation: EAW61897.1
BC126185 mRNA Translation: AAI26186.1
BC144061 mRNA Translation: AAI44062.1
AB015333 mRNA Translation: BAA34792.1
CCDSiCCDS4270.1 [Q9UBS8-1]
CCDS4271.1 [Q9UBS8-2]
RefSeqiNP_001188294.1, NM_001201365.1 [Q9UBS8-1]
NP_004281.1, NM_004290.4 [Q9UBS8-1]
NP_899645.1, NM_183398.2 [Q9UBS8-2]
NP_899646.1, NM_183399.2 [Q9UBS8-1]
NP_899647.1, NM_183400.2 [Q9UBS8-1]
NP_899648.1, NM_183401.2 [Q9UBS8-1]
XP_005268593.1, XM_005268536.2
XP_005268594.1, XM_005268537.2
XP_005268596.1, XM_005268539.2 [Q9UBS8-2]
XP_005268597.1, XM_005268540.3 [Q9UBS8-2]
XP_005268598.1, XM_005268541.3 [Q9UBS8-2]
XP_011536016.1, XM_011537714.2 [Q9UBS8-1]
XP_016865559.1, XM_017010070.1
XP_016865560.1, XM_017010071.1 [Q9UBS8-2]
XP_016865561.1, XM_017010072.1 [Q9UBS8-2]
UniGeneiHs.483616

3D structure databases

ProteinModelPortaliQ9UBS8
SMRiQ9UBS8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114968, 30 interactors
IntActiQ9UBS8, 18 interactors
MINTiQ9UBS8
STRINGi9606.ENSP00000324956

PTM databases

iPTMnetiQ9UBS8
PhosphoSitePlusiQ9UBS8

Polymorphism and mutation databases

BioMutaiRNF14
DMDMi17380293

Proteomic databases

EPDiQ9UBS8
PaxDbiQ9UBS8
PeptideAtlasiQ9UBS8
PRIDEiQ9UBS8
ProteomicsDBi84052

Protocols and materials databases

DNASUi9604
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000347642; ENSP00000324956; ENSG00000013561 [Q9UBS8-1]
ENST00000356143; ENSP00000348462; ENSG00000013561 [Q9UBS8-1]
ENST00000394514; ENSP00000378022; ENSG00000013561 [Q9UBS8-2]
ENST00000394519; ENSP00000378027; ENSG00000013561 [Q9UBS8-1]
ENST00000394520; ENSP00000378028; ENSG00000013561 [Q9UBS8-1]
GeneIDi9604
KEGGihsa:9604
UCSCiuc003lly.4 human [Q9UBS8-1]

Organism-specific databases

CTDi9604
DisGeNETi9604
EuPathDBiHostDB:ENSG00000013561.17
GeneCardsiRNF14
HGNCiHGNC:10058 RNF14
HPAiHPA008716
MIMi605675 gene
neXtProtiNX_Q9UBS8
OpenTargetsiENSG00000013561
PharmGKBiPA34423
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1814 Eukaryota
ENOG410Y5R1 LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000237334
HOVERGENiHBG057296
InParanoidiQ9UBS8
KOiK11971
OMAiGDIWEDE
OrthoDBiEOG091G08QR
PhylomeDBiQ9UBS8
TreeFamiTF314401

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiRNF14 human
GeneWikiiRNF14
GenomeRNAii9604
PROiPR:Q9UBS8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000013561 Expressed in 227 organ(s), highest expression level in testis
CleanExiHS_RNF14
ExpressionAtlasiQ9UBS8 baseline and differential
GenevisibleiQ9UBS8 HS

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR006575 RWD-domain
IPR016135 UBQ-conjugating_enzyme/RWD
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
PF05773 RWD, 1 hit
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00591 RWD, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50908 RWD, 1 hit
PS51873 TRIAD, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNF14_HUMAN
AccessioniPrimary (citable) accession number: Q9UBS8
Secondary accession number(s): A0AV26
, A6NMR2, A8MTW5, B3KN72, B7ZLV2, D3DQE4, O94793, Q6IBV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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