UniProtKB - Q9UBP6 (TRMB_HUMAN)
Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
METTL1
Organism
Homo sapiens (Human)
Status
Functioni
Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031084, PubMed:31031083). Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA (PubMed:12403464, PubMed:31031084). Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs (PubMed:31031084). Internal N7-methylguanine methylation of mRNAs regulates translation (PubMed:31031084). Also methylates a specific subset of miRNAs, such as let-7 (PubMed:31031083). N7-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed:31031083). Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).UniRule annotation3 Publications
Catalytic activityi
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.33UniRule annotation
- a guanosine in mRNA + S-adenosyl-L-methionine = an N7-methylguanosine in mRNA + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a guanosine in miRNA + S-adenosyl-L-methionine = an N7-methylguanosine in miRNA + S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 84 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 160 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 | |
Active sitei | 163 | UniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- RNA (guanine-N7)-methylation Source: GO_Central
- tRNA methylation Source: GO_Central
- tRNA modification Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | Q9UBP6 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
SIGNORi | Q9UBP6 |
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)Alternative name(s): |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7030, METTL1 |
MIMi | 604466, gene |
neXtProti | NX_Q9UBP6 |
VEuPathDBi | HostDB:ENSG00000037897.16 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- tRNA methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | S → A: Able to complement a trm8-delta mutant yeast. 1 Publication | 1 | |
Mutagenesisi | 27 | S → D or E: Abolishes ability to complement a trm8-delta mutant yeast. 1 Publication | 1 | |
Mutagenesisi | 107 – 109 | EIR → AAA: Abolished RNA methyltransferase activity. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 4234 |
OpenTargetsi | ENSG00000037897 |
PharmGKBi | PA30766 |
Miscellaneous databases
Pharosi | Q9UBP6, Tbio |
Genetic variation databases
BioMutai | METTL1 |
DMDMi | 32171926 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000171431 | 2 – 276 | tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST | 275 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 27 | Phosphoserine; by PKB and RPS6KA3UniRule annotationCombined sources1 Publication | 1 |
Post-translational modificationi
Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9UBP6 |
jPOSTi | Q9UBP6 |
MassIVEi | Q9UBP6 |
PaxDbi | Q9UBP6 |
PeptideAtlasi | Q9UBP6 |
PRIDEi | Q9UBP6 |
ProteomicsDBi | 43260 84026 [Q9UBP6-1] |
PTM databases
iPTMneti | Q9UBP6 |
PhosphoSitePlusi | Q9UBP6 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000037897, Expressed in left adrenal gland and 183 other tissues |
ExpressionAtlasi | Q9UBP6, baseline and differential |
Genevisiblei | Q9UBP6, HS |
Organism-specific databases
HPAi | ENSG00000037897, Low tissue specificity |
Interactioni
Subunit structurei
Forms a complex with WDR4.
1 PublicationBinary interactionsi
Q9UBP6
With | #Exp. | IntAct |
---|---|---|
WDR4 [P57081] | 13 | EBI-750415,EBI-750427 |
Protein-protein interaction databases
BioGRIDi | 110392, 38 interactors |
DIPi | DIP-61920N |
IntActi | Q9UBP6, 3 interactors |
STRINGi | 9606.ENSP00000314441 |
Miscellaneous databases
RNActi | Q9UBP6, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UBP6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UBP6 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 107 – 109 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 3 | |
Regioni | 140 – 141 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 | |
Regioni | 238 – 240 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
GeneTreei | ENSGT00390000017840 |
HOGENOMi | CLU_050910_3_0_1 |
InParanoidi | Q9UBP6 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
PhylomeDBi | Q9UBP6 |
TreeFami | TF314083 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9UBP6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY
60 70 80 90 100
PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD
110 120 130 140 150
TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY
160 170 180 190 200
KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV
210 220 230 240 250
LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK
260 270
NFPAIFRRIQ DPVLQAVTSQ TSLPGH
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VSD9 | F8VSD9_HUMAN | tRNA (guanine-N(7)-)-methyltransfer... | METTL1 | 36 | Annotation score: | ||
H0YHX0 | H0YHX0_HUMAN | tRNA (guanine-N(7)-)-methyltransfer... | METTL1 | 81 | Annotation score: | ||
H0YIH0 | H0YIH0_HUMAN | tRNA (guanine-N(7)-)-methyltransfer... | METTL1 | 124 | Annotation score: | ||
H0YHU4 | H0YHU4_HUMAN | tRNA (guanine-N(7)-)-methyltransfer... | METTL1 | 63 | Annotation score: |
Sequence cautioni
The sequence CAA65470 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | K → R in BAD96922 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044671 | 92 – 155 | VELSP…KGQLT → ADKDVLPLPRPTFQADKAQV ANHQSHPASRICLRAKSWGA GVYHNRCAGATRLDVHSFRR APTV in isoform 2. 1 PublicationAdd BLAST | 64 | |
Alternative sequenceiVSP_044672 | 156 – 276 | Missing in isoform 2. 1 PublicationAdd BLAST | 121 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18643 mRNA Translation: CAA77239.1 Y18642 Genomic DNA Translation: CAA77238.1 X96698 mRNA Translation: CAA65470.1 Different initiation. AK314851 mRNA Translation: BAG37368.1 AK223202 mRNA Translation: BAD96922.1 AC025165 Genomic DNA No translation available. BC000550 mRNA Translation: AAH00550.1 |
CCDSi | CCDS8955.3 [Q9UBP6-1] CCDS8956.1 [Q9UBP6-2] |
RefSeqi | NP_005362.3, NM_005371.5 [Q9UBP6-1] NP_075422.3, NM_023033.3 [Q9UBP6-2] |
Genome annotation databases
Ensembli | ENST00000257848; ENSP00000257848; ENSG00000037897 [Q9UBP6-2] ENST00000324871; ENSP00000314441; ENSG00000037897 [Q9UBP6-1] |
GeneIDi | 4234 |
KEGGi | hsa:4234 |
UCSCi | uc009zqc.4, human [Q9UBP6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18643 mRNA Translation: CAA77239.1 Y18642 Genomic DNA Translation: CAA77238.1 X96698 mRNA Translation: CAA65470.1 Different initiation. AK314851 mRNA Translation: BAG37368.1 AK223202 mRNA Translation: BAD96922.1 AC025165 Genomic DNA No translation available. BC000550 mRNA Translation: AAH00550.1 |
CCDSi | CCDS8955.3 [Q9UBP6-1] CCDS8956.1 [Q9UBP6-2] |
RefSeqi | NP_005362.3, NM_005371.5 [Q9UBP6-1] NP_075422.3, NM_023033.3 [Q9UBP6-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CKK | X-ray | 1.55 | A | 32-265 | [»] | |
SMRi | Q9UBP6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110392, 38 interactors |
DIPi | DIP-61920N |
IntActi | Q9UBP6, 3 interactors |
STRINGi | 9606.ENSP00000314441 |
PTM databases
iPTMneti | Q9UBP6 |
PhosphoSitePlusi | Q9UBP6 |
Genetic variation databases
BioMutai | METTL1 |
DMDMi | 32171926 |
Proteomic databases
EPDi | Q9UBP6 |
jPOSTi | Q9UBP6 |
MassIVEi | Q9UBP6 |
PaxDbi | Q9UBP6 |
PeptideAtlasi | Q9UBP6 |
PRIDEi | Q9UBP6 |
ProteomicsDBi | 43260 84026 [Q9UBP6-1] |
Protocols and materials databases
Antibodypediai | 16399, 167 antibodies |
DNASUi | 4234 |
Genome annotation databases
Ensembli | ENST00000257848; ENSP00000257848; ENSG00000037897 [Q9UBP6-2] ENST00000324871; ENSP00000314441; ENSG00000037897 [Q9UBP6-1] |
GeneIDi | 4234 |
KEGGi | hsa:4234 |
UCSCi | uc009zqc.4, human [Q9UBP6-1] |
Organism-specific databases
CTDi | 4234 |
DisGeNETi | 4234 |
GeneCardsi | METTL1 |
HGNCi | HGNC:7030, METTL1 |
HPAi | ENSG00000037897, Low tissue specificity |
MIMi | 604466, gene |
neXtProti | NX_Q9UBP6 |
OpenTargetsi | ENSG00000037897 |
PharmGKBi | PA30766 |
VEuPathDBi | HostDB:ENSG00000037897.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
GeneTreei | ENSGT00390000017840 |
HOGENOMi | CLU_050910_3_0_1 |
InParanoidi | Q9UBP6 |
OMAi | KWRIINQ |
OrthoDBi | 1417652at2759 |
PhylomeDBi | Q9UBP6 |
TreeFami | TF314083 |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
PathwayCommonsi | Q9UBP6 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
SIGNORi | Q9UBP6 |
Miscellaneous databases
BioGRID-ORCSi | 4234, 312 hits in 1008 CRISPR screens |
ChiTaRSi | METTL1, human |
EvolutionaryTracei | Q9UBP6 |
GeneWikii | METTL1 |
GenomeRNAii | 4234 |
Pharosi | Q9UBP6, Tbio |
PROi | PR:Q9UBP6 |
RNActi | Q9UBP6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000037897, Expressed in left adrenal gland and 183 other tissues |
ExpressionAtlasi | Q9UBP6, baseline and differential |
Genevisiblei | Q9UBP6, HS |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMB_HUMAN | |
Accessioni | Q9UBP6Primary (citable) accession number: Q9UBP6 Secondary accession number(s): B2RBX1 Q53FS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families