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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

METTL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei160S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Active sitei163UniRule annotation1

GO - Molecular functioni

  • tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • tRNA modification Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol
SIGNORiQ9UBP6
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:METTL1UniRule annotation
Synonyms:C12orf1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000037897.16
HGNCiHGNC:7030 METTL1
MIMi604466 gene
neXtProtiNX_Q9UBP6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27S → A: Able to complement a trm8-delta mutant yeast. 1 Publication1
Mutagenesisi27S → D or E: Abolishes ability to complement a trm8-delta mutant yeast. 1 Publication1

Organism-specific databases

DisGeNETi4234
OpenTargetsiENSG00000037897
PharmGKBiPA30766

Polymorphism and mutation databases

BioMutaiMETTL1
DMDMi32171926

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001714312 – 276tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei27Phosphoserine; by PKB and RPS6KA3UniRule annotationCombined sources1 Publication1

Post-translational modificationi

Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UBP6
PaxDbiQ9UBP6
PeptideAtlasiQ9UBP6
PRIDEiQ9UBP6
ProteomicsDBi84026

PTM databases

iPTMnetiQ9UBP6
PhosphoSitePlusiQ9UBP6

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000037897
CleanExiHS_METTL1
ExpressionAtlasiQ9UBP6 baseline and differential
GenevisibleiQ9UBP6 HS

Organism-specific databases

HPAiHPA020914
HPA050450

Interactioni

Subunit structurei

Forms a complex with WDR4.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
WDR4P5708110EBI-750415,EBI-750427

Protein-protein interaction databases

BioGridi110392, 37 interactors
DIPiDIP-61920N
IntActiQ9UBP6, 1 interactor
STRINGi9606.ENSP00000314441

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 43Combined sources3
Turni47 – 49Combined sources3
Beta strandi78 – 83Combined sources6
Helixi89 – 94Combined sources6
Helixi95 – 97Combined sources3
Beta strandi101 – 108Combined sources8
Helixi110 – 125Combined sources16
Beta strandi134 – 138Combined sources5
Turni141 – 143Combined sources3
Helixi145 – 148Combined sources4
Beta strandi154 – 161Combined sources8
Helixi178 – 187Combined sources10
Beta strandi188 – 199Combined sources12
Helixi201 – 212Combined sources12
Beta strandi217 – 220Combined sources4
Helixi222 – 225Combined sources4
Helixi231 – 233Combined sources3
Turni234 – 236Combined sources3
Helixi239 – 246Combined sources8
Beta strandi252 – 258Combined sources7
Turni262 – 264Combined sources3

3D structure databases

ProteinModelPortaliQ9UBP6
SMRiQ9UBP6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UBP6

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108S-adenosyl-L-methionine binding2
Regioni140 – 141S-adenosyl-L-methionine binding2
Regioni238 – 240S-adenosyl-L-methionine binding3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
GeneTreeiENSGT00390000017840
HOGENOMiHOG000169646
HOVERGENiHBG044569
InParanoidiQ9UBP6
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG091G0IQ7
PhylomeDBiQ9UBP6
TreeFamiTF314083

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UBP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY
60 70 80 90 100
PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD
110 120 130 140 150
TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY
160 170 180 190 200
KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV
210 220 230 240 250
LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK
260 270
NFPAIFRRIQ DPVLQAVTSQ TSLPGH
Length:276
Mass (Da):31,471
Last modified:May 1, 2000 - v1
Checksum:i715AE85A18632892
GO
Isoform 2 (identifier: Q9UBP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-155: VELSPLFPDT...PNFFYKGQLT → ADKDVLPLPR...VHSFRRAPTV
     156-276: Missing.

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,423
Checksum:iCFC2A730BA85E8D4
GO

Sequence cautioni

The sequence CAA65470 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18K → R in BAD96922 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04467192 – 155VELSP…KGQLT → ADKDVLPLPRPTFQADKAQV ANHQSHPASRICLRAKSWGA GVYHNRCAGATRLDVHSFRR APTV in isoform 2. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_044672156 – 276Missing in isoform 2. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18643 mRNA Translation: CAA77239.1
Y18642 Genomic DNA Translation: CAA77238.1
X96698 mRNA Translation: CAA65470.1 Different initiation.
AK314851 mRNA Translation: BAG37368.1
AK223202 mRNA Translation: BAD96922.1
AC025165 Genomic DNA No translation available.
BC000550 mRNA Translation: AAH00550.1
CCDSiCCDS8955.3 [Q9UBP6-1]
CCDS8956.1 [Q9UBP6-2]
RefSeqiNP_005362.3, NM_005371.5 [Q9UBP6-1]
NP_075422.3, NM_023033.3 [Q9UBP6-2]
UniGeneiHs.42957

Genome annotation databases

EnsembliENST00000257848; ENSP00000257848; ENSG00000037897 [Q9UBP6-2]
ENST00000324871; ENSP00000314441; ENSG00000037897 [Q9UBP6-1]
GeneIDi4234
KEGGihsa:4234
UCSCiuc009zqc.4 human [Q9UBP6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRMB_HUMAN
AccessioniPrimary (citable) accession number: Q9UBP6
Secondary accession number(s): B2RBX1
, H7BXF2, Q14105, Q53FS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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