PI4KB

Functionⁱ

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).By similarity3 Publications

Catalytic activityⁱ

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
ATP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
ADP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.1
  "Identification and characterization of a novel human phosphatidylinositol 4-kinase."
  Suzuki K., Hirano H., Okutomi K., Suzuki M., Kuga Y., Fujiwara T., Kanemoto N., Isono K., Horie M.
  DNA Res. 4:273-280(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
EC:
2.7.1.67
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.1
  "Identification and characterization of a novel human phosphatidylinositol 4-kinase."
  Suzuki K., Hirano H., Okutomi K., Suzuki M., Kuga Y., Fujiwara T., Kanemoto N., Isono K., Horie M.
  DNA Res. 4:273-280(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)
zoom
+
ATP
=
1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)
zoom
+
ADP
+
H⁺

Activity regulationⁱ

Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ (By similarity).By similarity

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS07046-MONOMER
BRENDAⁱ	2.7.1.67 2681
Reactomeⁱ	R-HSA-1660514 Synthesis of PIPs at the Golgi membrane
SIGNORⁱ	Q9UBF8

Chemistry databases

SwissLipidsⁱ	SLP:000001947 [Q9UBF8-2]

SwissLipidsⁱ

SLP:000001947 [Q9UBF8-2]

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phosphatidylinositol 4-kinase betaCurated (EC:2.7.1.67) Short name: PI4K-beta Short name: PI4Kbeta Short name: PtdIns 4-kinase beta Alternative name(s): NPIK PI4K92
Gene namesⁱ	Name:PI4KBImported Synonyms:PIK4CB
Organismⁱ	Homo sapiens (Human)Imported
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Unplaced

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:8984 PI4KB
MIMⁱ	602758 gene
neXtProtⁱ	NX_Q9UBF8

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	40 – 42	LSV → AAA: Small decrease in C10ORF76-binding. No effect on ACBD3-, nor RAB11B-binding. 1 Publication	3
Mutagenesisⁱ	42	V → A: No loss of interaction with ACBD3. 1 Publication	1
Mutagenesisⁱ	43 – 45	IDP → AAA: Drastically decreased ACBD3-binding. No effect on C10ORF76-, nor RAB11B-binding. 1 Publication	3
Mutagenesisⁱ	43	I → A: Loss of interaction with ACBD3. 2 Publications	1
Mutagenesisⁱ	44	D → A: Loss of interaction with ACBD3. 1 Publication	1
Mutagenesisⁱ	46 – 47	EV → AA: No effect on ACBD3-, C10ORF76-, nor RAB11B-binding. 1 Publication	2
Mutagenesisⁱ	47	V → A: No loss of interaction with ACBD3. 1 Publication	1
Mutagenesisⁱ	49 – 50	QK → AA: No effect on ACBD3-, C10ORF76-, nor RAB11B-binding. 1 Publication	2
Mutagenesisⁱ	52	C → A: No effect on ACBD3-, C10ORF76-, nor RAB11B-binding. 1 Publication	1
Mutagenesisⁱ	53 – 54	QE → AA: Drastically decreased C10ORF76- and RAB11B-binding. 6-fold increase in ACBD3-binding. 1 Publication	2
Mutagenesisⁱ	55 – 56	VL → AA: Drastically decreased ACBD3-binding. No effect on C10ORF76-, nor RAB11B-binding. 1 Publication	2
Mutagenesisⁱ	55	V → A: Loss of interaction with ACBD3. 1 Publication	1
Mutagenesisⁱ	56	L → A: Loss of interaction with ACBD3. 1 Publication	1
Mutagenesisⁱ	57 – 59	EKV → AAA: No effect on ACBD3-, C10ORF76-, nor RAB11B-binding. 1 Publication	3

Organism-specific databases

DisGeNET More...DisGeNETⁱ	5298
Open Targets More...OpenTargetsⁱ	ENSG00000143393
PharmGKBⁱ	PA162399420

Chemistry databases

ChEMBLⁱ	CHEMBL3268
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2149

Polymorphism and mutation databases

BioMutaⁱ	PI4KB
DMDMⁱ	38372507

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000088829	2 – 816	Phosphatidylinositol 4-kinase betaAdd BLAST		815

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylglycine1 Publication	1
Modified residueⁱ	258	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	263	Phosphothreonine1 Publication	1
Modified residueⁱ	266	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	275	PhosphoserineBy similarity	1
Modified residueⁱ	277	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	284	PhosphoserineBy similarity	1
Modified residueⁱ	294	Phosphoserine2 Publications	1
Modified residueⁱ	428	PhosphoserineCombined sources	1
Modified residueⁱ	438	Phosphothreonine1 Publication	1
Modified residueⁱ	511	PhosphoserineCombined sources1 Publication	1
Modified residueⁱ	517	PhosphothreonineCombined sources	1
Modified residueⁱ	519	Phosphothreonine1 Publication	1
Isoform 21 Publication (identifier: Q9UBF8-2)
Modified residueⁱ	294	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	Q9UBF8
jPOSTⁱ	Q9UBF8
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9UBF8
PaxDbⁱ	Q9UBF8
PeptideAtlas More...PeptideAtlasⁱ	Q9UBF8
PRIDEⁱ	Q9UBF8
ProteomicsDBⁱ	83959 83960 [Q9UBF8-2] 83961 [Q9UBF8-3]

PTM databases

iPTMnetⁱ	Q9UBF8
PhosphoSitePlusⁱ	Q9UBF8

Miscellaneous databases

PMAP-CutDBⁱ	Q9UBF8

PMAP-CutDBⁱ

Q9UBF8

Expressionⁱ

Tissue specificityⁱ

Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.3 Publications

Gene expression databases

Bgeeⁱ	ENSG00000143393 Expressed in 230 organ(s), highest expression level in left lobe of thyroid gland
ExpressionAtlasⁱ	Q9UBF8 baseline and differential
Genevisibleⁱ	Q9UBF8 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB022071 HPA006280 HPA006281

HPAⁱ

CAB022071
HPA006280
HPA006281

Interactionⁱ

Subunit structureⁱ

Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (PubMed:17555535).

Interacts with NCS1/FREQ in a calcium-independent manner.

Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity).

Interacts with ACBD3 (PubMed:27009356, PubMed:27989622, PubMed:23572552).

Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (PubMed:23572552).

Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane (PubMed:28289207).

By similarity5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ACBD3	Q9H3P7	4	EBI-1053214,EBI-1791792
NS	P03495	2	EBI-1053214,EBI-2548993	From Influenza A virus (strain A/Udorn/307/1972 H3N2).
RAB11A	P62491	5	EBI-16107849,EBI-745098

GO - Molecular functionⁱ

14-3-3 protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- Ref.8
  "ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."
  Greninger A.L., Knudsen G.M., Betegon M., Burlingame A.L., DeRisi J.L.
  MBio 4:E00098-E00098(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 2-118; 121-229; 236-303; 319-442; 497-798 AND 804-816 (ISOFORM 2), CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT GLY-2, PHOSPHORYLATION AT SER-294, INTERACTION WITH ACBD3; ARMH3; RAB11B; YWHAB; YWHAE; YWHAG; YWHAH; YWHAQ; YWHAZ AND SFN, MUTAGENESIS OF 40-LEU--VAL-42; 43-ILE--PRO-45; 46-GLU-VAL-47; 49-GLN-LYS-50; CYS-52; 53-GLN-GLU-54; 55-VAL-LEU-56 AND 57-GLU--VAL-59, MASS SPECTROMETRY.

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	111316, 22 interactors
Database of interacting proteins More...DIPⁱ	DIP-42267N
IntActⁱ	Q9UBF8, 12 interactors
Molecular INTeraction database More...MINTⁱ	Q9UBF8
STRINGⁱ	9606.ENSP00000357869

Chemistry databases

BindingDBⁱ

Q9UBF8

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	45 – 63	Combined sources	19
Helixⁱ	129 – 134	Combined sources	6
Helixⁱ	141 – 150	Combined sources	10
Helixⁱ	154 – 163	Combined sources	10
Helixⁱ	164 – 166	Combined sources	3
Helixⁱ	169 – 173	Combined sources	5
Helixⁱ	176 – 185	Combined sources	10
Helixⁱ	188 – 204	Combined sources	17
Helixⁱ	206 – 218	Combined sources	13
Helixⁱ	234 – 242	Combined sources	9
Turnⁱ	319 – 322	Combined sources	4
Helixⁱ	323 – 338	Combined sources	16
Helixⁱ	339 – 341	Combined sources	3
Beta strandⁱ	342 – 344	Combined sources	3
Helixⁱ	345 – 356	Combined sources	12
Turnⁱ	357 – 359	Combined sources	3
Helixⁱ	360 – 362	Combined sources	3
Beta strandⁱ	364 – 366	Combined sources	3
Beta strandⁱ	372 – 374	Combined sources	3
Beta strandⁱ	376 – 380	Combined sources	5
Helixⁱ	383 – 385	Combined sources	3
Beta strandⁱ	392 – 394	Combined sources	3
Beta strandⁱ	397 – 405	Combined sources	9
Turnⁱ	409 – 411	Combined sources	3
Helixⁱ	537 – 546	Combined sources	10
Turnⁱ	549 – 552	Combined sources	4
Beta strandⁱ	556 – 564	Combined sources	9
Helixⁱ	570 – 588	Combined sources	19
Beta strandⁱ	600 – 602	Combined sources	3
Beta strandⁱ	604 – 606	Combined sources	3
Beta strandⁱ	608 – 610	Combined sources	3
Beta strandⁱ	614 – 618	Combined sources	5
Helixⁱ	619 – 625	Combined sources	7
Helixⁱ	630 – 637	Combined sources	8
Helixⁱ	644 – 667	Combined sources	24
Turnⁱ	674 – 676	Combined sources	3
Beta strandⁱ	677 – 680	Combined sources	4
Beta strandⁱ	685 – 687	Combined sources	3
Helixⁱ	712 – 717	Combined sources	6
Beta strandⁱ	721 – 723	Combined sources	3
Helixⁱ	724 – 741	Combined sources	18
Helixⁱ	744 – 755	Combined sources	12
Helixⁱ	761 – 763	Combined sources	3
Beta strandⁱ	765 – 767	Combined sources	3
Helixⁱ	768 – 774	Combined sources	7
Beta strandⁱ	778 – 780	Combined sources	3
Helixⁱ	782 – 796	Combined sources	15

Show more details

3D structure databases

SMRⁱ	Q9UBF8
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 242	PIK helicalPROSITE-ProRule annotationAdd BLAST		214
Domainⁱ	558 – 765	PI3K/PI4KPROSITE-ProRule annotationCuratedAdd BLAST		208

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	2 – 68	Interaction with ACBD31 PublicationAdd BLAST		67

Sequence similaritiesⁱ

Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0903 Eukaryota ENOG410XPH3 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074892
InParanoidⁱ	Q9UBF8
KEGG Orthology (KO) More...KOⁱ	K19801
Database of Orthologous Groups More...OrthoDBⁱ	1147978at2759
PhylomeDBⁱ	Q9UBF8
TreeFamⁱ	TF102042

Family and domain databases

Gene3Dⁱ	1.10.1070.11, 1 hit
InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000403 PI3/4_kinase_cat_dom IPR036940 PI3/4_kinase_cat_sf IPR018936 PI3/4_kinase_CS IPR001263 PI3K_accessory_dom IPR015433 PI_Kinase
PANTHERⁱ	PTHR10048 PTHR10048, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00454 PI3_PI4_kinase, 1 hit
SMARTⁱ	View protein in SMART SM00146 PI3Kc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00915 PI3_4_KINASE_1, 1 hit PS00916 PI3_4_KINASE_2, 1 hit PS50290 PI3_4_KINASE_3, 1 hit PS51545 PIK_HELICAL, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show all Align All

Isoform 11 Publication (identifier: Q9UBF8-1) [UniParc]FASTA Add to basket

Also known as: NPIK-B1 Publication

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK 
        60         70         80         90        100
ACQEVLEKVK LLHGGVAVSS RGTPLELVNG DGVDSEIRCL DDPPAQIREE 
       110        120        130        140        150
EDEMGAAVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN 
       160        170        180        190        200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH 
       210        220        230        240        250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR 
       260        270        280        290        300
KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 
       310        320        330        340        350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ 
       360        370        380        390        400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY 
       410        420        430        440        450
VEVLECENFD TTSVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP 
       460        470        480        490        500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF 
       510        520        530        540        550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY 
       560        570        580        590        600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 
       610        620        630        640        650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ 
       660        670        680        690        700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN 
       710        720        730        740        750
LGFETSAFKL TTEFVDVMGG LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI 
       760        770        780        790        800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI 
       810 
TTKLYDGFQY LTNGIM

Length:816

Mass (Da):91,379

Last modified:May 1, 2000 - v1

Checksum:ⁱCAD8791729BB4308

GO

Isoform 21 Publication (identifier: Q9UBF8-2) [UniParc]FASTA Add to basket

Also known as: NPIK-C1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
304-318: Missing.

« Hide

        10         20         30         40         50
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK 
        60         70         80         90        100
ACQEVLEKVK LLHGGVAVSS RGTPLELVNG DGVDSEIRCL DDPPAQIREE 
       110        120        130        140        150
EDEMGAAVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN 
       160        170        180        190        200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH 
       210        220        230        240        250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR 
       260        270        280        290        300
KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 
       310        320        330        340        350
EDEPVRLAPE REFIKSLMAI GKRLATLPTK EQKTQRLISE LSLLNHKLPA 
       360        370        380        390        400
RVWLPTAGFD HHVVRVPHTQ AVVLNSKDKA PYLIYVEVLE CENFDTTSVP 
       410        420        430        440        450
ARIPENRIRS TRSVENLPEC GITHEQRAGS FSTVPNYDND DEAWSVDDIG 
       460        470        480        490        500
ELQVELPEVH TNSCDNISQF SVDSITSQES KEPVFIAAGD IRRRLSEQLA 
       510        520        530        540        550
HTPTAFKRDP EDPSAVALKE PWQEKVRRIR EGSPYGHLPN WRLLSVIVKC 
       560        570        580        590        600
GDDLRQELLA FQVLKQLQSI WEQERVPLWI KPYKILVISA DSGMIEPVVN 
       610        620        630        640        650
AVSIHQVKKQ SQLSLLDYFL QEHGSYTTEA FLSAQRNFVQ SCAGYCLVCY 
       660        670        680        690        700
LLQVKDRHNG NILLDAEGHI IHIDFGFILS SSPRNLGFET SAFKLTTEFV 
       710        720        730        740        750
DVMGGLDGDM FNYYKMLMLQ GLIAARKHMD KVVQIVEIMQ QGSQLPCFHG 
       760        770        780        790        800
SSTIRNLKER FHMSMTEEQL QLLVEQMVDG SMRSITTKLY DGFQYLTNGI 

M

Show »

Length:801

Mass (Da):89,807

Checksum:ⁱ22E1ABA37AA9A2BD

GO

Isoform 3 (identifier: Q9UBF8-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-332: Missing.

« Hide

        10         20         30         40         50
MAIGKRLATL PTKEQKTQRL ISELSLLNHK LPARVWLPTA GFDHHVVRVP 
        60         70         80         90        100
HTQAVVLNSK DKAPYLIYVE VLECENFDTT SVPARIPENR IRSTRSVENL 
       110        120        130        140        150
PECGITHEQR AGSFSTVPNY DNDDEAWSVD DIGELQVELP EVHTNSCDNI 
       160        170        180        190        200
SQFSVDSITS QESKEPVFIA AGDIRRRLSE QLAHTPTAFK RDPEDPSAVA 
       210        220        230        240        250
LKEPWQEKVR RIREGSPYGH LPNWRLLSVI VKCGDDLRQE LLAFQVLKQL 
       260        270        280        290        300
QSIWEQERVP LWIKPYKILV ISADSGMIEP VVNAVSIHQV KKQSQLSLLD 
       310        320        330        340        350
YFLQEHGSYT TEAFLSAQRN FVQSCAGYCL VCYLLQVKDR HNGNILLDAE 
       360        370        380        390        400
GHIIHIDFGF ILSSSPRNLG FETSAFKLTT EFVDVMGGLD GDMFNYYKML 
       410        420        430        440        450
MLQGLIAARK HMDKVVQIVE IMQQGSQLPC FHGSSTIRNL KERFHMSMTE 
       460        470        480 
EQLQLLVEQM VDGSMRSITT KLYDGFQYLT NGIM

Show »

Length:484

Mass (Da):54,976

Checksum:ⁱ395A9DF64FC58A8C

GO

Computationally mapped potential isoform sequencesⁱ

There are 7 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A0B4J1S8	A0A0B4J1S8_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	828	Annotation score:
A6PVV8	A6PVV8_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	227	Annotation score:
H0YCW3	H0YCW3_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	183	Annotation score:
H0Y4F8	H0Y4F8_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	73	Annotation score:
A0A0A0MSL0	A0A0A0MSL0_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	280	Annotation score:
F8W860	F8W860_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	191	Annotation score:
E9PL47	E9PL47_HUMAN	Phosphatidylinositol 4-kinase beta Phosphatidylinositol 4-kinase beta	PI4KB	95	Annotation score:

Sequence cautionⁱ

The sequence BAA21661 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence EAW53450 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	216	L → V in AAC51156 (PubMed:9020160).Curated	1
Sequence conflictⁱ	339	L → V in AAC51156 (PubMed:9020160).Curated	1
Sequence conflictⁱ	340	A → V in AAC51156 (PubMed:9020160).Curated	1
Sequence conflictⁱ	370	P → S in AAC51156 (PubMed:9020160).Curated	1
Sequence conflictⁱ	446	F → S in BAG57793 (PubMed:14702039).Curated	1
Sequence conflictⁱ	754	M → L in BAG57793 (PubMed:14702039).Curated	1
Sequence conflictⁱ	796	S → G in BAG57793 (PubMed:14702039).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_037133	1 – 332	Missing in isoform 3. 1 PublicationAdd BLAST		332
Alternative sequenceⁱVSP_050627	304 – 318	Missing in isoform 2. 3 PublicationsAdd BLAST		15

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ011121 mRNA Translation: CAA09495.1 AJ011122 mRNA No translation available. AJ011123 mRNA Translation: CAA09496.1 AB005910 mRNA Translation: BAA21661.1 Different initiation. U81802 mRNA Translation: AAC51156.1 AK294606 mRNA Translation: BAG57793.1 AL391069 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53449.1 CH471121 Genomic DNA Translation: EAW53450.1 Sequence problems. BC000029 mRNA Translation: AAH00029.1 BC040300 mRNA Translation: AAH40300.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS55637.1 [Q9UBF8-3] CCDS55638.1 [Q9UBF8-2] CCDS81376.1 [Q9UBF8-1]
PIRⁱ	JC5706
NCBI Reference Sequences More...RefSeqⁱ	NP_001185702.1, NM_001198773.2 [Q9UBF8-2] NP_001185703.1, NM_001198774.1 [Q9UBF8-2] NP_001185704.1, NM_001198775.2 [Q9UBF8-3] NP_001317650.1, NM_001330721.1 [Q9UBF8-1] NP_002642.1, NM_002651.3 XP_016856979.1, XM_017001490.1 [Q9UBF8-1] XP_016856980.1, XM_017001491.1 [Q9UBF8-1] XP_016856981.1, XM_017001492.1 [Q9UBF8-2]

Genome annotation databases

GeneIDⁱ	5298
KEGGⁱ	hsa:5298
UCSC genome browser More...UCSCⁱ	uc001ext.4 human [Q9UBF8-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9UBF8	Phosphatidylinositol 4-kinase beta		HUMAN		828	UniRef100_Q9UBF8
Q9UBF8-3	Phosphatidylinositol 4-kinase beta		CALJA		816	UniRef100_B0KWC1
phosphatidylinositol 4-kinase beta isoform X3		TARSY		484
PI4KB isoform 4		PONAB		484
PI4KB isoform 4		PANTR		484
Phosphatidylinositol 4-kinase beta		CALJA		848

Protein	Similar proteins		Species	Score	Length	Source
Q9UBF8	Phosphatidylinositol 4-kinase beta		RAT		816	UniRef90_Q9UBF8
Phosphatidylinositol 4-kinase beta		PAPAN		816
Phosphatidylinositol 4-kinase beta		MOUSE		816
Phosphatidylinositol 4-kinase beta	)	BOVIN		816
Phosphatidylinositol 4-kinase beta		OTOGA		816
+360

Protein	Similar proteins		Species	Score	Length	Source
Q9UBF8	Phosphatidylinositol 4-kinase beta		PAPAN		816	UniRef50_Q9UBF8
Phosphatidylinositol 4-kinase beta		RAT		816
Phosphatidylinositol 4-kinase beta		MOUSE		816
Phosphatidylinositol 4-kinase beta		CALJA		816
Phosphatidylinositol 4-kinase beta	)	BOVIN		816
+760

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ011121 mRNA Translation: CAA09495.1 AJ011122 mRNA No translation available. AJ011123 mRNA Translation: CAA09496.1 AB005910 mRNA Translation: BAA21661.1 Different initiation. U81802 mRNA Translation: AAC51156.1 AK294606 mRNA Translation: BAG57793.1 AL391069 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53449.1 CH471121 Genomic DNA Translation: EAW53450.1 Sequence problems. BC000029 mRNA Translation: AAH00029.1 BC040300 mRNA Translation: AAH40300.1
CCDSⁱ	CCDS55637.1 [Q9UBF8-3] CCDS55638.1 [Q9UBF8-2] CCDS81376.1 [Q9UBF8-1]
PIRⁱ	JC5706
RefSeqⁱ	NP_001185702.1, NM_001198773.2 [Q9UBF8-2] NP_001185703.1, NM_001198774.1 [Q9UBF8-2] NP_001185704.1, NM_001198775.2 [Q9UBF8-3] NP_001317650.1, NM_001330721.1 [Q9UBF8-1] NP_002642.1, NM_002651.3 XP_016856979.1, XM_017001490.1 [Q9UBF8-1] XP_016856980.1, XM_017001491.1 [Q9UBF8-1] XP_016856981.1, XM_017001492.1 [Q9UBF8-2]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2N73	NMR	-	B	1-68	[»]
	4D0L	X-ray	2.94	A/C/E	121-799	[»]
	4D0M	X-ray	6.00	A/C/G/I/M/O/Q/S/W/Y/c/g	121-799	[»]
	4WAE	X-ray	3.32	A	128-799	[»]
	4WAG	X-ray	3.41	A	128-799	[»]
	5C46	X-ray	2.65	E	121-799	[»]
	5C4G	X-ray	3.20	E	121-799	[»]
	5EUQ	X-ray	3.20	E	121-248	[»]
				E	523-799	[»]
	5FBL	X-ray	3.37	A	128-799	[»]
	5FBQ	X-ray	3.79	A	128-799	[»]
	5FBR	X-ray	3.28	A	128-799	[»]
	5FBV	X-ray	3.29	A	128-799	[»]
	5FBW	X-ray	3.49	A	128-799	[»]
	5LX2	X-ray	2.58	B	292-297	[»]
	5NAS	X-ray	2.08	C/D	289-297	[»]
	6GL3	X-ray	2.77	A/B	317-428	[»]
				A/B	532-798	[»]
SMRⁱ	Q9UBF8
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	111316, 22 interactors
DIPⁱ	DIP-42267N
IntActⁱ	Q9UBF8, 12 interactors
MINTⁱ	Q9UBF8
STRINGⁱ	9606.ENSP00000357869

Chemistry databases

BindingDBⁱ	Q9UBF8
ChEMBLⁱ	CHEMBL3268
GuidetoPHARMACOLOGYⁱ	2149
SwissLipidsⁱ	SLP:000001947 [Q9UBF8-2]

PTM databases

iPTMnetⁱ	Q9UBF8
PhosphoSitePlusⁱ	Q9UBF8

Polymorphism and mutation databases

BioMutaⁱ	PI4KB
DMDMⁱ	38372507

Proteomic databases

EPDⁱ	Q9UBF8
jPOSTⁱ	Q9UBF8
MaxQBⁱ	Q9UBF8
PaxDbⁱ	Q9UBF8
PeptideAtlasⁱ	Q9UBF8
PRIDEⁱ	Q9UBF8
ProteomicsDBⁱ	83959 83960 [Q9UBF8-2] 83961 [Q9UBF8-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	5298
Structural Biology Knowledgebase	Search...

Genome annotation databases

GeneIDⁱ	5298
KEGGⁱ	hsa:5298
UCSCⁱ	uc001ext.4 human [Q9UBF8-1]

Organism-specific databases

CTDⁱ	5298
DisGeNETⁱ	5298
GeneCardsⁱ	PI4KB
H-InvDBⁱ	HIX0001056
HGNCⁱ	HGNC:8984 PI4KB
HPAⁱ	CAB022071 HPA006280 HPA006281
MIMⁱ	602758 gene
neXtProtⁱ	NX_Q9UBF8
OpenTargetsⁱ	ENSG00000143393
PharmGKBⁱ	PA162399420
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0903 Eukaryota ENOG410XPH3 LUCA
GeneTreeⁱ	ENSGT00550000074892
InParanoidⁱ	Q9UBF8
KOⁱ	K19801
OrthoDBⁱ	1147978at2759
PhylomeDBⁱ	Q9UBF8
TreeFamⁱ	TF102042

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS07046-MONOMER
BRENDAⁱ	2.7.1.67 2681
Reactomeⁱ	R-HSA-1660514 Synthesis of PIPs at the Golgi membrane
SIGNORⁱ	Q9UBF8

Miscellaneous databases

ChiTaRSⁱ	PI4KB human
GeneWikiⁱ	PI4KB
GenomeRNAiⁱ	5298
PMAP-CutDBⁱ	Q9UBF8
Protein Ontology More...PROⁱ	PR:Q9UBF8
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000143393 Expressed in 230 organ(s), highest expression level in left lobe of thyroid gland
ExpressionAtlasⁱ	Q9UBF8 baseline and differential
Genevisibleⁱ	Q9UBF8 HS

Family and domain databases

Gene3Dⁱ	1.10.1070.11, 1 hit
InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000403 PI3/4_kinase_cat_dom IPR036940 PI3/4_kinase_cat_sf IPR018936 PI3/4_kinase_CS IPR001263 PI3K_accessory_dom IPR015433 PI_Kinase
PANTHERⁱ	PTHR10048 PTHR10048, 1 hit
Pfamⁱ	View protein in Pfam PF00454 PI3_PI4_kinase, 1 hit
SMARTⁱ	View protein in SMART SM00146 PI3Kc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00915 PI3_4_KINASE_1, 1 hit PS00916 PI3_4_KINASE_2, 1 hit PS50290 PI3_4_KINASE_3, 1 hit PS51545 PIK_HELICAL, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PI4KB_HUMAN
Accessionⁱ	Q9UBF8Primary (citable) accession number: Q9UBF8 Secondary accession number(s): B4DGI2 Q9BWR6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
	Last sequence update:	May 1, 2000
	Last modified:	July 3, 2019
	This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM

UniProtKB

UniParc

Help

UniRef

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Supporting data

UniProtKB - Q9UBF8 (PI4KB_HUMAN)

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Phosphatidylinositol 4-kinase beta

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Golgi apparatus

Endoplasmic reticulum

Mitochondrion

Other locations

Cytosol

Endoplasmic reticulum

Endosome

Golgi apparatus

Mitochondrion

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ