UniProtKB - Q9UBE0 (SAE1_HUMAN)
SUMO-activating enzyme subunit 1
SAE1
Functioni
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
GO - Molecular functioni
- ATP-dependent protein binding Source: UniProtKB
- enzyme activator activity Source: ProtInc
- protein C-terminus binding Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
- small protein activating enzyme binding Source: CAFA
- ubiquitin activating enzyme activity Source: UniProtKB
GO - Biological processi
- positive regulation of protein sumoylation Source: UniProtKB
- positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
- protein modification by small protein conjugation Source: GO_Central
- protein sumoylation Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q9UBE0 |
Reactomei | R-HSA-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-HSA-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) |
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: SUMO-activating enzyme subunit 1Alternative name(s): Ubiquitin-like 1-activating enzyme E1A Cleaved into the following chain: |
Gene namesi | Name:SAE1 Synonyms:AOS1, SUA1, UBLE1A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30660, SAE1 |
MIMi | 613294, gene |
neXtProti | NX_Q9UBE0 |
VEuPathDBi | HostDB:ENSG00000142230.11 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- SUMO activating enzyme complex Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | R → A: Abolishes ATP-dependent activation of SUMO proteins. 1 Publication | 1 | |
Mutagenesisi | 24 – 26 | RLW → AAA: Abolishes ATP-dependent activation of SUMO proteins. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 10055 |
OpenTargetsi | ENSG00000142230 |
PharmGKBi | PA162402387 |
Miscellaneous databases
Pharosi | Q9UBE0, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1615388 |
DrugCentrali | Q9UBE0 |
Genetic variation databases
BioMutai | SAE1 |
DMDMi | 42559897 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194966 | 1 – 346 | SUMO-activating enzyme subunit 1Add BLAST | 346 | |
Initiator methioninei | Removed; alternateCombined sources1 Publication | |||
ChainiPRO_0000423290 | 2 – 346 | SUMO-activating enzyme subunit 1, N-terminally processedAdd BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 2 | N-acetylvaline; in SUMO-activating enzyme subunit 1, N-terminally processedCombined sources1 Publication | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 198 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9UBE0 |
jPOSTi | Q9UBE0 |
MassIVEi | Q9UBE0 |
MaxQBi | Q9UBE0 |
PaxDbi | Q9UBE0 |
PeptideAtlasi | Q9UBE0 |
PRIDEi | Q9UBE0 |
ProteomicsDBi | 24559 33772 83952 [Q9UBE0-1] |
PTM databases
iPTMneti | Q9UBE0 |
MetOSitei | Q9UBE0 |
PhosphoSitePlusi | Q9UBE0 |
SwissPalmi | Q9UBE0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000142230, Expressed in ventricular zone and 236 other tissues |
ExpressionAtlasi | Q9UBE0, baseline and differential |
Genevisiblei | Q9UBE0, HS |
Organism-specific databases
HPAi | ENSG00000142230, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1.
Interacts with UBE2I.
2 PublicationsBinary interactionsi
Hide detailsQ9UBE0
GO - Molecular functioni
- ATP-dependent protein binding Source: UniProtKB
- protein C-terminus binding Source: UniProtKB
- protein heterodimerization activity Source: UniProtKB
- small protein activating enzyme binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 115366, 86 interactors |
ComplexPortali | CPX-2161, SUMO activating enzyme complex CPX-3042, activated SUMO1-E1 ligase complex CPX-3044, activated SUMO2-E1 ligase complex CPX-3045, activated SUMO3-E1 ligase complex CPX-3076, activated SUMO4-E1 ligase complex |
CORUMi | Q9UBE0 |
DIPi | DIP-34587N |
IntActi | Q9UBE0, 37 interactors |
MINTi | Q9UBE0 |
STRINGi | 9606.ENSP00000270225 |
Chemistry databases
BindingDBi | Q9UBE0 |
Miscellaneous databases
RNActi | Q9UBE0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9UBE0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9UBE0 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2014, Eukaryota |
GeneTreei | ENSGT00550000075007 |
InParanoidi | Q9UBE0 |
OMAi | MEAPHRN |
PhylomeDBi | Q9UBE0 |
TreeFami | TF315037 |
Family and domain databases
DisProti | DP00485 |
IDEALi | IID00397 |
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz IPR000011, UBQ/SUMO-activ_enz_E1-like |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
PRINTSi | PR01849, UBIQUITINACT |
SUPFAMi | SSF69572, SSF69572, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVEKEEAGGG ISEEEAAQYD RQIRLWGLEA QKRLRASRVL LVGLKGLGAE
60 70 80 90 100
IAKNLILAGV KGLTMLDHEQ VTPEDPGAQF LIRTGSVGRN RAEASLERAQ
110 120 130 140 150
NLNPMVDVKV DTEDIEKKPE SFFTQFDAVC LTCCSRDVIV KVDQICHKNS
160 170 180 190 200
IKFFTGDVFG YHGYTFANLG EHEFVEEKTK VAKVSQGVED GPDTKRAKLD
210 220 230 240 250
SSETTMVKKK VVFCPVKEAL EVDWSSEKAK AALKRTTSDY FLLQVLLKFR
260 270 280 290 300
TDKGRDPSSD TYEEDSELLL QIRNDVLDSL GISPDLLPED FVRYCFSEMA
310 320 330 340
PVCAVVGGIL AQEIVKALSQ RDPPHNNFFF FDGMKGNGIV ECLGPK
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0QX65 | M0QX65_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 205 | Annotation score: | ||
M0QZS6 | M0QZS6_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 265 | Annotation score: | ||
M0QYM8 | M0QYM8_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 133 | Annotation score: | ||
M0R054 | M0R054_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 157 | Annotation score: | ||
M0R286 | M0R286_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 98 | Annotation score: | ||
A0A590UK93 | A0A590UK93_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 210 | Annotation score: | ||
B3KNJ4 | B3KNJ4_HUMAN | SUMO-1 activating enzyme subunit 1,... | SAE1 hCG_20373 | 299 | Annotation score: | ||
A0A590UKC5 | A0A590UKC5_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 235 | Annotation score: | ||
M0QYP2 | M0QYP2_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 71 | Annotation score: | ||
M0R375 | M0R375_HUMAN | SUMO-activating enzyme subunit 1 | SAE1 | 50 | Annotation score: | ||
There is more potential isoformShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | I → M in BAG51064 (Ref. 6) Curated | 1 | |
Sequence conflicti | 158 | V → A in AAF29104 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 178 – 180 | KTK → ETD in AAF29104 (PubMed:11042152).Curated | 3 | |
Sequence conflicti | 186 | Q → H in AAF29104 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 273 | R → G in AAD12785 (PubMed:9920803).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045372 | 245 – 299 | VLLKF…CFSEM → GPVSAGPSSQQLLLLRWHEG EWDCGVPWPQVNSRFGSPRD ANCSMPTCIPCPLPS in isoform 3. 1 PublicationAdd BLAST | 55 | |
Alternative sequenceiVSP_045373 | 245 – 266 | VLLKF…YEEDS → GTASPRWPQCVRWLEGFWHR KL in isoform 2. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_045374 | 267 – 346 | Missing in isoform 2. 1 PublicationAdd BLAST | 80 | |
Alternative sequenceiVSP_045375 | 300 – 346 | Missing in isoform 3. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF090385 mRNA Translation: AAD12785.2 AF046025 mRNA Translation: AAD23902.2 AF110956 mRNA Translation: AAD24433.1 AF161489 mRNA Translation: AAF29104.1 AL560234 mRNA No translation available. BT007290 mRNA Translation: AAP35954.1 AK021978 mRNA Translation: BAG51064.1 AK315624 mRNA Translation: BAG37992.1 AC008532 Genomic DNA No translation available. AC008755 Genomic DNA No translation available. CH471126 Genomic DNA Translation: EAW57461.1 CH471126 Genomic DNA Translation: EAW57463.1 BC000344 mRNA Translation: AAH00344.1 BC003611 mRNA Translation: AAH03611.1 BC018271 mRNA Translation: AAH18271.1 |
CCDSi | CCDS12696.1 [Q9UBE0-1] CCDS54284.1 [Q9UBE0-2] CCDS54285.1 [Q9UBE0-3] |
RefSeqi | NP_001139185.1, NM_001145713.1 [Q9UBE0-3] NP_001139186.1, NM_001145714.1 [Q9UBE0-2] NP_005491.1, NM_005500.2 [Q9UBE0-1] |
Genome annotation databases
Ensembli | ENST00000270225; ENSP00000270225; ENSG00000142230 [Q9UBE0-1] ENST00000392776; ENSP00000440818; ENSG00000142230 [Q9UBE0-2] ENST00000413379; ENSP00000416557; ENSG00000142230 [Q9UBE0-3] |
GeneIDi | 10055 |
KEGGi | hsa:10055 |
UCSCi | uc002pgc.4, human [Q9UBE0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF090385 mRNA Translation: AAD12785.2 AF046025 mRNA Translation: AAD23902.2 AF110956 mRNA Translation: AAD24433.1 AF161489 mRNA Translation: AAF29104.1 AL560234 mRNA No translation available. BT007290 mRNA Translation: AAP35954.1 AK021978 mRNA Translation: BAG51064.1 AK315624 mRNA Translation: BAG37992.1 AC008532 Genomic DNA No translation available. AC008755 Genomic DNA No translation available. CH471126 Genomic DNA Translation: EAW57461.1 CH471126 Genomic DNA Translation: EAW57463.1 BC000344 mRNA Translation: AAH00344.1 BC003611 mRNA Translation: AAH03611.1 BC018271 mRNA Translation: AAH18271.1 |
CCDSi | CCDS12696.1 [Q9UBE0-1] CCDS54284.1 [Q9UBE0-2] CCDS54285.1 [Q9UBE0-3] |
RefSeqi | NP_001139185.1, NM_001145713.1 [Q9UBE0-3] NP_001139186.1, NM_001145714.1 [Q9UBE0-2] NP_005491.1, NM_005500.2 [Q9UBE0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Y8Q | X-ray | 2.25 | A/C | 1-346 | [»] | |
1Y8R | X-ray | 2.75 | A/D | 1-346 | [»] | |
3KYC | X-ray | 2.45 | A | 1-346 | [»] | |
3KYD | X-ray | 2.61 | A | 1-346 | [»] | |
6CWY | X-ray | 2.46 | C | 1-346 | [»] | |
6CWZ | X-ray | 3.10 | C | 1-346 | [»] | |
SMRi | Q9UBE0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115366, 86 interactors |
ComplexPortali | CPX-2161, SUMO activating enzyme complex CPX-3042, activated SUMO1-E1 ligase complex CPX-3044, activated SUMO2-E1 ligase complex CPX-3045, activated SUMO3-E1 ligase complex CPX-3076, activated SUMO4-E1 ligase complex |
CORUMi | Q9UBE0 |
DIPi | DIP-34587N |
IntActi | Q9UBE0, 37 interactors |
MINTi | Q9UBE0 |
STRINGi | 9606.ENSP00000270225 |
Chemistry databases
BindingDBi | Q9UBE0 |
ChEMBLi | CHEMBL1615388 |
DrugCentrali | Q9UBE0 |
PTM databases
iPTMneti | Q9UBE0 |
MetOSitei | Q9UBE0 |
PhosphoSitePlusi | Q9UBE0 |
SwissPalmi | Q9UBE0 |
Genetic variation databases
BioMutai | SAE1 |
DMDMi | 42559897 |
Proteomic databases
EPDi | Q9UBE0 |
jPOSTi | Q9UBE0 |
MassIVEi | Q9UBE0 |
MaxQBi | Q9UBE0 |
PaxDbi | Q9UBE0 |
PeptideAtlasi | Q9UBE0 |
PRIDEi | Q9UBE0 |
ProteomicsDBi | 24559 33772 83952 [Q9UBE0-1] |
Protocols and materials databases
Antibodypediai | 18168, 526 antibodies |
DNASUi | 10055 |
Genome annotation databases
Ensembli | ENST00000270225; ENSP00000270225; ENSG00000142230 [Q9UBE0-1] ENST00000392776; ENSP00000440818; ENSG00000142230 [Q9UBE0-2] ENST00000413379; ENSP00000416557; ENSG00000142230 [Q9UBE0-3] |
GeneIDi | 10055 |
KEGGi | hsa:10055 |
UCSCi | uc002pgc.4, human [Q9UBE0-1] |
Organism-specific databases
CTDi | 10055 |
DisGeNETi | 10055 |
GeneCardsi | SAE1 |
HGNCi | HGNC:30660, SAE1 |
HPAi | ENSG00000142230, Low tissue specificity |
MIMi | 613294, gene |
neXtProti | NX_Q9UBE0 |
OpenTargetsi | ENSG00000142230 |
PharmGKBi | PA162402387 |
VEuPathDBi | HostDB:ENSG00000142230.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2014, Eukaryota |
GeneTreei | ENSGT00550000075007 |
InParanoidi | Q9UBE0 |
OMAi | MEAPHRN |
PhylomeDBi | Q9UBE0 |
TreeFami | TF315037 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
PathwayCommonsi | Q9UBE0 |
Reactomei | R-HSA-3065676, SUMO is conjugated to E1 (UBA2:SAE1) R-HSA-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) |
Miscellaneous databases
BioGRID-ORCSi | 10055, 712 hits in 1010 CRISPR screens |
ChiTaRSi | SAE1, human |
EvolutionaryTracei | Q9UBE0 |
GeneWikii | SAE1 |
GenomeRNAii | 10055 |
Pharosi | Q9UBE0, Tbio |
PROi | PR:Q9UBE0 |
RNActi | Q9UBE0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142230, Expressed in ventricular zone and 236 other tissues |
ExpressionAtlasi | Q9UBE0, baseline and differential |
Genevisiblei | Q9UBE0, HS |
Family and domain databases
DisProti | DP00485 |
IDEALi | IID00397 |
InterProi | View protein in InterPro IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz IPR000011, UBQ/SUMO-activ_enz_E1-like |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
PRINTSi | PR01849, UBIQUITINACT |
SUPFAMi | SSF69572, SSF69572, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAE1_HUMAN | |
Accessioni | Q9UBE0Primary (citable) accession number: Q9UBE0 Secondary accession number(s): B2RDP5 Q9P020 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families