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Protein

Transcription factor grauzone

Gene

grau

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor essential for the completion of meiosis in oocytes. Grauzone binds to the promoter region of cort via the zf-AD domain and activates cort expression in ovaries.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi4Zinc 11
Metal bindingi7Zinc 21
Metal bindingi53Zinc 31
Metal bindingi56Zinc 41

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri240 – 263C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri296 – 318C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri327 – 349C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri387 – 409C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri417 – 441C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri448 – 471C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri476 – 498C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri504 – 527C2H2-type 8PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • proximal promoter sequence-specific DNA binding Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processMeiosis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor grauzone
Gene namesi
Name:grau
ORF Names:CG3282
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001133 grau

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi298C → Y in QQ36; defects in female meiosis. Abolishes DNA-binding activity. 1
Mutagenesisi493E → K in QE70; defects in female meiosis. Abolishes DNA-binding activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000469231 – 570Transcription factor grauzoneAdd BLAST570

Proteomic databases

PaxDbiQ9U405
PRIDEiQ9U405

Expressioni

Tissue specificityi

Present throughout embryogenesis, in larvae, in ovaries and other tissues of adult females, and in adult males. In ovaries, it is expressed in both follicle cells and nurse cells. Expressed from stage 9 in the germarium. Weakly or not expressed in the oocyte nucleus.1 Publication

Developmental stagei

Expressed both maternally and zygotically.

Gene expression databases

BgeeiFBgn0001133 Expressed in 28 organ(s), highest expression level in embryo
ExpressionAtlasiQ9U405 baseline and differential
GenevisibleiQ9U405 DM

Interactioni

Subunit structurei

Homodimer; at the zf-AD.1 Publication

Protein-protein interaction databases

BioGridi69943, 15 interactors
DIPiDIP-20107N
IntActiQ9U405, 3 interactors
STRINGi7227.FBpp0071477

Structurei

Secondary structure

1570
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9U405
SMRiQ9U405
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9U405

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 80ZADAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni293 – 570DNA-bindingAdd BLAST278

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi383 – 399Bipartite nuclear localization signal 1Sequence analysisAdd BLAST17
Motifi523 – 539Bipartite nuclear localization signal 2Sequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi146 – 172Asp/Glu-rich (acidic)Add BLAST27

Domaini

The N-terminal zf-AD is an atypical treble-clef like zinc binding domain which enables dimerization, and appears not to have any DNA-binding ability. DNA binding is achieved through the zinc fingers at the C-terminus.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri240 – 263C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri296 – 318C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri327 – 349C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri387 – 409C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri417 – 441C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri448 – 471C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri476 – 498C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri504 – 527C2H2-type 8PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00920000149281
HOGENOMiHOG000038877
InParanoidiQ9U405
OMAiDEECDFQ
OrthoDBiEOG091G02KC
PhylomeDBiQ9U405

Family and domain databases

InterProiView protein in InterPro
IPR012934 Znf_AD
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF07776 zf-AD, 1 hit
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00868 zf-AD, 1 hit
SM00355 ZnF_C2H2, 9 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits

Sequencei

Sequence statusi: Complete.

Q9U405-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDICRLCLRG VSGAQMCLQI FDVDSGESKV AEVLRQHFWF EVLPNDEISK
60 70 80 90 100
VICNVCWTQV SEFHQFYVSI QEAQVIYATT SKFKQDPEMV NTSWPEEVLM
110 120 130 140 150
PADVLAVDND VGAQINVNPL DELDLSQPMS PEDSKVGIKT ERQSPDMELL
160 170 180 190 200
FEDANNEQDE DYEDDEDDDT DDLIVTRSGR KRKRDVAKPA KTKRGTVSVG
210 220 230 240 250
RKGKEKMVVK RGPPKRIFKM ERLPPFCKED EELIKRYIVM GCELCIFLAE
260 270 280 290 300
DFDGIREHFK DKHPDERPYI KCCGRKLNKR CLIQEHARRH ENPEYIKCKD
310 320 330 340 350
CGKVFANSSV LRAHWLVHHV PDEECDFQCE DCGKRFSRRN LLELHKGSHV
360 370 380 390 400
PVNERKFICP QCPKHNAFAT EYHMQVHISM QHRKAANICH VCGKKIKDKA
410 420 430 440 450
VFEKHVRLHF EESGPRIKCP RPDCESWLKD EDNLKQHLRR HNDEGKLFIC
460 470 480 490 500
SECGKSCKNS RALIGHKRYS HSNVIYTCEQ CGKTFKKDIS LKEHMAQHTG
510 520 530 540 550
EPLYKCPFCP RTFNSNANMH SHKKKMHPVE WDIWRKTKTG SSQKVLPSAQ
560 570
VAQMFRDDAD VAAIANDYSG
Length:570
Mass (Da):65,967
Last modified:May 1, 2000 - v1
Checksum:i33B550B964C1EA7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208016 mRNA Translation: AAF25356.1
AE013599 Genomic DNA Translation: AAF46653.2
AY047537 mRNA Translation: AAK77269.1
RefSeqiNP_788422.1, NM_176242.3
UniGeneiDm.19597

Genome annotation databases

EnsemblMetazoaiFBtr0071548; FBpp0071477; FBgn0001133
GeneIDi45871
KEGGidme:Dmel_CG33133
UCSCiCG33133-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF208016 mRNA Translation: AAF25356.1
AE013599 Genomic DNA Translation: AAF46653.2
AY047537 mRNA Translation: AAK77269.1
RefSeqiNP_788422.1, NM_176242.3
UniGeneiDm.19597

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PZWX-ray2.00A2-81[»]
ProteinModelPortaliQ9U405
SMRiQ9U405
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69943, 15 interactors
DIPiDIP-20107N
IntActiQ9U405, 3 interactors
STRINGi7227.FBpp0071477

Proteomic databases

PaxDbiQ9U405
PRIDEiQ9U405

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0071548; FBpp0071477; FBgn0001133
GeneIDi45871
KEGGidme:Dmel_CG33133
UCSCiCG33133-RA d. melanogaster

Organism-specific databases

CTDi45871
FlyBaseiFBgn0001133 grau

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00920000149281
HOGENOMiHOG000038877
InParanoidiQ9U405
OMAiDEECDFQ
OrthoDBiEOG091G02KC
PhylomeDBiQ9U405

Miscellaneous databases

EvolutionaryTraceiQ9U405
GenomeRNAii45871
PROiPR:Q9U405

Gene expression databases

BgeeiFBgn0001133 Expressed in 28 organ(s), highest expression level in embryo
ExpressionAtlasiQ9U405 baseline and differential
GenevisibleiQ9U405 DM

Family and domain databases

InterProiView protein in InterPro
IPR012934 Znf_AD
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF07776 zf-AD, 1 hit
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00868 zf-AD, 1 hit
SM00355 ZnF_C2H2, 9 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGRAU_DROME
AccessioniPrimary (citable) accession number: Q9U405
Secondary accession number(s): Q9W2N7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: May 1, 2000
Last modified: October 10, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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