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Protein

Glycogen synthase kinase-3

Gene

gsk-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates oma-1, a regulator of the oocyte-to-embryo transition, enabling its degradation (PubMed:16343905, PubMed:16289132). Phosphorylates skn-1, preventing it from accumulating in nuclei and thus inhibiting phase II gene expression in the oxidative stress defense (PubMed:16251270). Involved in mesendoderm specification and mitotic spindle orientation in EMS blastomeres (PubMed:11463373). Thought to be a branch point in these processes as proteins downstream are not required (PubMed:10444600). Negatively regulates Wnt signaling in vulval precursor cells and acts as a Wnt-independent repressor of med-1 and med-2 in the C lineage inhibiting mesoderm development (PubMed:10444600, PubMed:11463373, PubMed:15572126). Required for normal lifespan and LiCl-induced lifespan extension (PubMed:17959600).8 Publications

Catalytic activityi

ATP + [tau protein] = ADP + [tau protein] phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65ATPPROSITE-ProRule annotation1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 50ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: WormBase
  • tau-protein kinase activity Source: UniProtKB-EC

GO - Biological processi

  • engulfment of apoptotic cell Source: WormBase
  • establishment of mitotic spindle orientation Source: UniProtKB
  • germ cell development Source: WormBase
  • left/right axis specification Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • peptidyl-threonine phosphorylation Source: WormBase
  • positive regulation of distal tip cell migration Source: UniProtKB
  • positive regulation of engulfment of apoptotic cell Source: UniProtKB
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: WormBase
  • Wnt signaling pathway, regulating spindle positioning Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processStress response, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-195253 Degradation of beta-catenin by the destruction complex
R-CEL-3371453 Regulation of HSF1-mediated heat shock response
SignaLinkiQ9U2Q9

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen synthase kinase-31 Publication (EC:2.7.11.26)
Gene namesi
Name:gsk-3Imported
Synonyms:sgg-11 Publication
ORF Names:Y18D10A.5Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiY18D10A.5 ; CE21401 ; WBGene00001746 ; gsk-3

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Worms exhibit defects in endoderm specification and mitotic spindle alignment (PubMed:10444600). RNAi-mediated knockdown results in stability and delayed degradation of the maternal oocyte protein oma-1 in embryos beyond the 2-cell stage (PubMed:16289132).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003491361 – 362Glycogen synthase kinase-3Add BLAST362

Proteomic databases

EPDiQ9U2Q9
PaxDbiQ9U2Q9
PeptideAtlasiQ9U2Q9
PRIDEiQ9U2Q9

Expressioni

Gene expression databases

BgeeiWBGene00001746

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with axl-1.By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi38546, 22 interactors
DIPiDIP-25216N
IntActiQ9U2Q9, 14 interactors
MINTiQ9U2Q9
STRINGi6239.Y18D10A.5.2

Structurei

3D structure databases

ProteinModelPortaliQ9U2Q9
SMRiQ9U2Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 320Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0658 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00520000055635
HOGENOMiHOG000233017
InParanoidiQ9U2Q9
KOiK03083
OMAiHARRHCG
OrthoDBiEOG091G099S
PhylomeDBiQ9U2Q9

Family and domain databases

CDDicd14137 STKc_GSK3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR039192 STKc_GSK3
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9U2Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKQLLSCSL KSGKQVTMVV ASVATDGVDQ QVEISYYDQK VIGNGSFGVV
60 70 80 90 100
FLAKLSTTNE MVAIKKVLQD KRFKNRELQI MRKLNHPNIV KLKYFFYSSG
110 120 130 140 150
EKKDELYLNL ILEYVPETVY RVARHYSKQR QQIPMIYVKL YMYQLLRSLA
160 170 180 190 200
YIHSIGICHR DIKPQNLLID PESGVLKLCD FGSAKYLVRN EPNVSYICSR
210 220 230 240 250
YYRAPELIFG ATNYTNSIDV WSAGTVMAEL LLGQPIFPGD SGVDQLVEII
260 270 280 290 300
KVLGTPTREQ IQSMNPNYKE FKFPQIKAHP WNKVFRVHTP AEAIDLISKI
310 320 330 340 350
IEYTPTSRPT PQAACQHAFF DELRNPDARL PSGRPLPTLE MDGPMGTGEV
360
STTSGDVAGP SA
Length:362
Mass (Da):40,882
Last modified:May 1, 2000 - v1
Checksum:iABDD2F49CC475BF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350V → I in AAD45354 (PubMed:10444600).Curated1
Sequence conflicti352T → P in AAD45354 (PubMed:10444600).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159950 mRNA Translation: AAD45354.1
AL034393 Genomic DNA Translation: CAA22311.1
PIRiT26520
RefSeqiNP_493243.1, NM_060842.5
UniGeneiCel.7399

Genome annotation databases

EnsemblMetazoaiY18D10A.5; Y18D10A.5; WBGene00001746
GeneIDi173149
KEGGicel:CELE_Y18D10A.5
UCSCiY18D10A.5 c. elegans

Similar proteinsi

Entry informationi

Entry nameiGSK3_CAEEL
AccessioniPrimary (citable) accession number: Q9U2Q9
Secondary accession number(s): Q9Y0C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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