UniProtKB - Q9TXJ0 (CMK1_CAEEL)
Protein
Calcium/calmodulin-dependent protein kinase type 1
Gene
cmk-1
Organism
Caenorhabditis elegans
Status
Functioni
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade which results in transcriptional activation (PubMed:10428833, PubMed:12231504, PubMed:26725111). Transcriptional activation occurs at least in part through phosphorylation of crh-1 (PubMed:10428833, PubMed:12231504). Regulates gene expression, sensory morphology, and function of the AFD thermosensory neurons (PubMed:10428833, PubMed:14711416, PubMed:25467978). Involved in long-term adaptation of AFD neurons to temperatures warmer than the initial acclimatized cultivation temperature (PubMed:25467978). Acts in the FLP thermal nociceptors to moderate the responsiveness to noxious heat and controls neuropeptide release from FLP neurons in response to temperature elevations (PubMed:25467982). Regulates the dauer decision, the decision of the larvae to enter into the alternative stress-resistant and long-lived dauer developmental stage, based on the feeding state, primarily in the AWC sensory neurons. Acts non cell-autonomously in the AWC neurons to regulate expression of the daf-28 insulin-like peptide and cell-autonomously in the ASI sensory neurons to regulate expression of the growth promoting daf-7 in a food-regulated manner (PubMed:26335407). Plays a role in memory-based thermal response of an individual AFD neuron cell (PubMed:26725111). Involved in chemotaxis response in AWC neurons to attractant 2-heptanone, a volatile organic compound emitted by the nematode pathogenic bacterium B.nematocida B16 (PubMed:27660389). Represses transcription of glutamate receptor glr-1 in the nucleus basally and in response to change in synaptic activity (PubMed:27462879).9 Publications
Catalytic activityi
Cofactori
Mg2+2 Publications
Activity regulationi
Activated by Ca2+/calmodulin (PubMed:10428833, PubMed:26725111). Binding of calmodulin results in a conformational change that generates functional binding sites for both substrate and ATP, and thus relieves autoinhibition and lowers the Km of substrate binding. Must be phosphorylated by ckk-1 to be maximally active but this does not appear to be required for activity in AFD neurons (PubMed:10428833).2 Publications
Kineticsi
- KM=657 µM for syntide-2 (when cmk-1 is not phosphorylated on Thr-179)1 Publication
- KM=20 µM for syntide-2 (when cmk-1 is phosphorylated on Thr-179)1 Publication
- Vmax=0.1 mmol/min/mg enzyme (irrespective of phosphorylation status of Thr-179)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 52 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 144 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 28 – 36 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: WormBase
- calmodulin-dependent protein kinase activity Source: WormBase
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- neuron projection morphogenesis Source: WormBase
- positive regulation of CREB transcription factor activity Source: WormBase
- positive regulation of transcription by RNA polymerase II Source: WormBase
- protein autophosphorylation Source: WormBase
- protein phosphorylation Source: WormBase
- regulation of neuron differentiation Source: WormBase
- thermosensory behavior Source: WormBase
Keywordsi
Molecular function | Allosteric enzyme, Calmodulin-binding, Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-CEL-9619229 Activation of RAC1 downstream of NMDARs |
SABIO-RKi | Q9TXJ0 |
SignaLinki | Q9TXJ0 |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium/calmodulin-dependent protein kinase type 11 Publication (EC:2.7.11.173 Publications)Alternative name(s): CaM kinase I1 Publication Short name: CaM-KI1 Publication |
Gene namesi | Name:cmk-1 ORF Names:K07A9.2 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | K07A9.2 ; CE25046 ; WBGene00000553 ; cmk-1 |
Subcellular locationi
Nucleus
- Nucleus 5 Publications
Other locations
- Cytoplasm 4 Publications
Note: Localization is regulated by temperature in thermosensory neurons (PubMed:25467978, PubMed:25467982). Localizes to the nucleus upon elevated temperature shift (PubMed:25467978). Translocates from cytoplasm to the nucleus of FLP thermal nociceptors during thermal adaptation (PubMed:25467982). Translocates transiently from cytoplasm to the nucleus of AWC sensory neurons in response to food withdrawal. Prolonged starvation results in cytoplasmic enrichment (PubMed:26335407). The distribution between cytoplasm and nucleus is regulated by synaptic glutamate receptor glr-1 levels in PVC neurons, accumulating in the nucleus with increasing glr-1 (PubMed:27462879).4 Publications
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Changes in thermosensory behavior and temperature-dependent defects in AFD-specific gene expression (PubMed:10428833, PubMed:14711416, PubMed:25467978, PubMed:26725111). Decreased threshold for temperature-evoked activity in the AFD neurons. Defects in negative thermotaxis and isothermal tracking behaviors (PubMed:25467978). Defective in thermal avoidance adaptation. Aberrant thermotaxis at innocuous temperatures (PubMed:25467982). Inappropriate dauer formation under well-fed conditions. Reduced expression of growth promoting daf-7 in ASI sensory neurons (PubMed:26335407). Changes in thermosensory response of AIY neurons (PubMed:26725111). Increased transcription of glutamate receptor glr-1 (PubMed:27462879).7 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 – 7 | Missing : Loss of nuclear localization. 1 Publication | 6 | |
Mutagenesisi | 52 | K → A: Loss of function in cytoplasm and nucleus in FLP thermal nociceptors but no effect on temperature-dependent translocation from cytoplasm to the nucleus. Increased transcription of glutamate receptor glr-1. 2 Publications | 1 | |
Mutagenesisi | 52 | K → M: Loss of kinase activity. 1 Publication | 1 | |
Mutagenesisi | 179 | T → A: Loss of phosphorylation and activation by ckk-1. No effect on function in AFD neurons. Strongly enriched in the cytoplasm regardless of cultivation temperature. Fails to rescue the AFD thermosensory neurons defects of cmk-1 null mutant. Loss of temperature-dependent translocation from cytoplasm to the nucleus during heat acclimation in FLP thermal nociceptors. 4 Publications | 1 | |
Mutagenesisi | 179 | T → D: Increase in basal kinase activity. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000395335 | 1 – 348 | Calcium/calmodulin-dependent protein kinase type 1Add BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 179 | Phosphothreonine; by ckk-11 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9TXJ0 |
PaxDbi | Q9TXJ0 |
PeptideAtlasi | Q9TXJ0 |
PRIDEi | Q9TXJ0 |
PTM databases
iPTMneti | Q9TXJ0 |
Expressioni
Tissue specificityi
Expressed in head and tail neurons and vulval muscles (PubMed:12231504). Throughout the nervous system. Detected in neurites and neuronal cell bodies (PubMed:25467982).2 Publications
Gene expression databases
Bgeei | WBGene00000553 Expressed in 4 organ(s), highest expression level in multi-cellular organism |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: WormBase
Protein-protein interaction databases
BioGridi | 42145, 1 interactor |
DIPi | DIP-26403N |
IntActi | Q9TXJ0, 1 interactor |
STRINGi | 6239.K07A9.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 278 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 257 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 318 | Autoinhibitory domainBy similarityAdd BLAST | 41 | |
Regioni | 298 – 319 | Calmodulin-bindingBy similarityAdd BLAST | 22 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 2 – 7 | Nuclear localization signal1 Publication | 6 |
Domaini
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.By similarity
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Sequence analysis
Phylogenomic databases
eggNOGi | KOG0032 Eukaryota ENOG410XRMJ LUCA |
GeneTreei | ENSGT00940000156378 |
HOGENOMi | HOG000233016 |
InParanoidi | Q9TXJ0 |
KOi | K08794 |
OMAi | QVQPCRY |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q9TXJ0 |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9TXJ0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLFKRRDGS GPAPNATIRE KYDFRDVLGT GAFSKVFLAE SKSDAGQMYA
60 70 80 90 100
VKCIDKKALK GKEESLENEI KVLRKLRHNN IVQLFDTYDE KQFVYLVMEL
110 120 130 140 150
VTGGELFDRI VAKGSYTEQD ASNLIRQVLE AVGFMHDNGV VHRDLKPENL
160 170 180 190 200
LYYNQDEDSK IMISDFGLSK TEDSGVMATA CGTPGYVAPE VLQQKPYGKA
210 220 230 240 250
VDVWSIGVIA YILLCGYPPF YDESDANLFA QIIKGEYEFD APYWDQISDS
260 270 280 290 300
AKDFITHLMC CDPEARFTCQ DALSHPWISG NTAYTHDIHG TVAVHLKKSL
310 320 330 340
AKRNWKKAYN AAAAIRQLQM LRLSSNSNRL QKQASQQQPE PPTPAFHA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 249 | D → G in BAA82674 (PubMed:10428833).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021864 mRNA Translation: BAA82674.1 FO081474 Genomic DNA Translation: CCD71865.1 AY350451 mRNA Translation: AAQ54691.1 |
RefSeqi | NP_500139.1, NM_067738.6 |
Genome annotation databases
EnsemblMetazoai | K07A9.2.1; K07A9.2.1; WBGene00000553 |
GeneIDi | 176989 |
KEGGi | cel:CELE_K07A9.2 |
UCSCi | K07A9.2 c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021864 mRNA Translation: BAA82674.1 FO081474 Genomic DNA Translation: CCD71865.1 AY350451 mRNA Translation: AAQ54691.1 |
RefSeqi | NP_500139.1, NM_067738.6 |
3D structure databases
SMRi | Q9TXJ0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 42145, 1 interactor |
DIPi | DIP-26403N |
IntActi | Q9TXJ0, 1 interactor |
STRINGi | 6239.K07A9.2 |
PTM databases
iPTMneti | Q9TXJ0 |
Proteomic databases
EPDi | Q9TXJ0 |
PaxDbi | Q9TXJ0 |
PeptideAtlasi | Q9TXJ0 |
PRIDEi | Q9TXJ0 |
Genome annotation databases
EnsemblMetazoai | K07A9.2.1; K07A9.2.1; WBGene00000553 |
GeneIDi | 176989 |
KEGGi | cel:CELE_K07A9.2 |
UCSCi | K07A9.2 c. elegans |
Organism-specific databases
CTDi | 176989 |
WormBasei | K07A9.2 ; CE25046 ; WBGene00000553 ; cmk-1 |
Phylogenomic databases
eggNOGi | KOG0032 Eukaryota ENOG410XRMJ LUCA |
GeneTreei | ENSGT00940000156378 |
HOGENOMi | HOG000233016 |
InParanoidi | Q9TXJ0 |
KOi | K08794 |
OMAi | QVQPCRY |
OrthoDBi | 330091at2759 |
PhylomeDBi | Q9TXJ0 |
Enzyme and pathway databases
Reactomei | R-CEL-9619229 Activation of RAC1 downstream of NMDARs |
SABIO-RKi | Q9TXJ0 |
SignaLinki | Q9TXJ0 |
Miscellaneous databases
PROi | PR:Q9TXJ0 |
Gene expression databases
Bgeei | WBGene00000553 Expressed in 4 organ(s), highest expression level in multi-cellular organism |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CMK1_CAEEL | |
Accessioni | Q9TXJ0Primary (citable) accession number: Q9TXJ0 Secondary accession number(s): Q6V5R5, Q9UAH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 13, 2010 |
Last sequence update: | May 1, 2000 | |
Last modified: | November 13, 2019 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase