UniProtKB - Q9TU47 (IF6_BOVIN)
Protein
Eukaryotic translation initiation factor 6
Gene
EIF6
Organism
Bos taurus (Bovine)
Status
Functioni
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (By similarity). In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).UniRule annotationBy similarity
GO - Molecular functioni
- ribosomal large subunit binding Source: GO_Central
- ribosome binding Source: InterPro
- translation initiation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- assembly of large subunit precursor of preribosome Source: GO_Central
- gene silencing by miRNA Source: UniProtKB
- maturation of 5.8S rRNA Source: GO_Central
- maturation of LSU-rRNA Source: GO_Central
- mature ribosome assembly Source: UniProtKB-UniRule
- miRNA mediated inhibition of translation Source: UniProtKB
- positive regulation of translation Source: UniProtKB
- regulation of fatty acid biosynthetic process Source: UniProtKB
- regulation of glycolytic process Source: UniProtKB
- regulation of megakaryocyte differentiation Source: UniProtKB
- regulation of reactive oxygen species metabolic process Source: UniProtKB
- response to insulin Source: UniProtKB
- ribosomal subunit export from nucleus Source: GO_Central
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 6UniRule annotationShort name: eIF-6UniRule annotation Alternative name(s): Imc-415 homolog |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:57006, EIF6 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleolus Source: GO_Central
Other locations
- preribosome, large subunit precursor Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000259426 | 1 – 245 | Eukaryotic translation initiation factor 6Add BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 113 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 165 | PhosphothreonineUniRule annotationBy similarity | 1 | |
Modified residuei | 166 | PhosphoserineUniRule annotationBy similarity | 1 | |
Modified residuei | 174 | Phosphoserine; by CK1UniRule annotationBy similarity | 1 | |
Modified residuei | 175 | Phosphoserine; by CK1UniRule annotationBy similarity | 1 | |
Modified residuei | 235 | Phosphoserine; by PKCUniRule annotationBy similarity | 1 | |
Modified residuei | 239 | PhosphoserineUniRule annotationBy similarity | 1 | |
Modified residuei | 243 | PhosphoserineUniRule annotationBy similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.By similarity
Ufmylated by UFL1.By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q9TU47 |
PRIDEi | Q9TU47 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000011263, Expressed in placenta and 18 other tissues |
ExpressionAtlasi | Q9TU47, baseline and differential |
Interactioni
Subunit structurei
Monomer. Associates with the 60S ribosomal subunit.
Interacts with RACK1 (By similarity).
Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC) (By similarity).
UniRule annotationBy similarityProtein-protein interaction databases
IntActi | Q9TU47, 2 interactors |
STRINGi | 9913.ENSBTAP00000014966 |
Family & Domainsi
Sequence similaritiesi
Belongs to the eIF-6 family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3185, Eukaryota |
GeneTreei | ENSGT00390000015972 |
HOGENOMi | CLU_071894_0_0_1 |
InParanoidi | Q9TU47 |
OMAi | GEDTTGP |
OrthoDBi | 1157302at2759 |
TreeFami | TF105396 |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9TU47-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVRASFENN CEIGCFAKLT NSYCLVAIGG SENFYSVFEG ELAGTIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNCLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAQPS TIATSMRDSL IDSLT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A452DIG6 | A0A452DIG6_BOVIN | Eukaryotic translation initiation f... | EIF6 ITGB4BP | 248 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 141 | T → K in AAF00595 (PubMed:10879479).Curated | 1 | |
Sequence conflicti | 198 | V → A in AAF00595 (PubMed:10879479).Curated | 1 | |
Sequence conflicti | 210 | T → A in AAF00595 (PubMed:10879479).Curated | 1 | |
Sequence conflicti | 222 – 224 | FKL → SKP in AAF00595 (PubMed:10879479).Curated | 3 | |
Sequence conflicti | 241 | I → T in AAF00595 (PubMed:10879479).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF141872 mRNA Translation: AAF00595.1 BC109922 mRNA Translation: AAI09923.1 |
PIRi | JC7273 |
RefSeqi | NP_777255.1, NM_174830.1 XP_005214560.1, XM_005214503.3 |
Genome annotation databases
Ensembli | ENSBTAT00000076570; ENSBTAP00000062338; ENSBTAG00000011263 |
GeneIDi | 286811 |
KEGGi | bta:286811 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF141872 mRNA Translation: AAF00595.1 BC109922 mRNA Translation: AAI09923.1 |
PIRi | JC7273 |
RefSeqi | NP_777255.1, NM_174830.1 XP_005214560.1, XM_005214503.3 |
3D structure databases
SMRi | Q9TU47 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9TU47, 2 interactors |
STRINGi | 9913.ENSBTAP00000014966 |
Proteomic databases
PaxDbi | Q9TU47 |
PRIDEi | Q9TU47 |
Genome annotation databases
Ensembli | ENSBTAT00000076570; ENSBTAP00000062338; ENSBTAG00000011263 |
GeneIDi | 286811 |
KEGGi | bta:286811 |
Organism-specific databases
CTDi | 3692 |
VGNCi | VGNC:57006, EIF6 |
Phylogenomic databases
eggNOGi | KOG3185, Eukaryota |
GeneTreei | ENSGT00390000015972 |
HOGENOMi | CLU_071894_0_0_1 |
InParanoidi | Q9TU47 |
OMAi | GEDTTGP |
OrthoDBi | 1157302at2759 |
TreeFami | TF105396 |
Gene expression databases
Bgeei | ENSBTAG00000011263, Expressed in placenta and 18 other tissues |
ExpressionAtlasi | Q9TU47, baseline and differential |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF6_BOVIN | |
Accessioni | Q9TU47Primary (citable) accession number: Q9TU47 Secondary accession number(s): Q2TBM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | October 31, 2006 | |
Last modified: | December 2, 2020 | |
This is version 103 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Translation initiation factors
List of translation initiation factor entries