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Protein

Photosystem II protein D1

Gene

psbA

Organism
Alexandrium tamarense (Red tide dinoflagellate) (Gonyaulax tamarensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Photosystem II (PSII) is a light-driven water: plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbA) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.UniRule annotation

Miscellaneous

2 of the reaction center chlorophylls (ChlD1 and ChlD2) are entirely coordinated by water.UniRule annotation
Herbicides such as atrazine, BNT, diuron or ioxynil bind in the Q(B) binding site and block subsequent electron transfer.UniRule annotation

Catalytic activityi

2 H2O + 2 plastoquinone + 4 light = O2 + 2 plastoquinol.UniRule annotation

Cofactori

Note: The D1/D2 heterodimer binds P680, chlorophylls that are the primary electron donor of PSII, and subsequent electron acceptors. It shares a non-heme iron and each subunit binds pheophytin, quinone, additional chlorophylls, carotenoids and lipids. D1 provides most of the ligands for the Mn4-Ca-O5 cluster of the oxygen-evolving complex (OEC). There is also a Cl(-1) ion associated with D1 and D2, which is required for oxygen evolution. The PSII complex binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120Magnesium (chlorophyll-a ChlzD1 axial ligand); via tele nitrogenUniRule annotation1
Binding sitei128Pheophytin D1UniRule annotation1
Sitei163Tyrosine radical intermediateUniRule annotation1
Metal bindingi172Calcium-manganese-oxide [Ca-4Mn-5O]; calciumUniRule annotation1
Metal bindingi172Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi191Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Sitei192Stabilizes free radical intermediateUniRule annotation1
Metal bindingi200Magnesium (chlorophyll-a PD1 axial ligand); via tele nitrogenUniRule annotation1
Metal bindingi217Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Binding sitei217Quinone (B)UniRule annotation1
Metal bindingi274Iron; shared with heterodimeric partner; via tele nitrogenUniRule annotation1
Metal bindingi334Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi335Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 3UniRule annotation1
Metal bindingi335Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 4UniRule annotation1
Metal bindingi344Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 1UniRule annotation1
Metal bindingi344Calcium-manganese-oxide [Ca-4Mn-5O]; manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Herbicide resistance, Photosynthesis, Transport
LigandCalcium, Chlorophyll, Chromophore, Iron, Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II protein D1UniRule annotation (EC:1.10.3.9UniRule annotation)
Short name:
PSII D1 proteinUniRule annotation
Alternative name(s):
Photosystem II Q(B) proteinUniRule annotation
Gene namesi
Name:psbAUniRule annotation
Encoded oniPlastid; Chloroplast
OrganismiAlexandrium tamarense (Red tide dinoflagellate) (Gonyaulax tamarensis)
Taxonomic identifieri2926 [NCBI]
Taxonomic lineageiEukaryotaAlveolataDinophyceaeGonyaulacalesGonyaulacaceaeAlexandrium

Subcellular locationi

  • chloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 48HelicalUniRule annotationAdd BLAST18
Transmembranei120 – 135HelicalUniRule annotationAdd BLAST16
Transmembranei144 – 158HelicalUniRule annotationAdd BLAST15
Transmembranei199 – 220HelicalUniRule annotationAdd BLAST22
Transmembranei276 – 290HelicalUniRule annotationAdd BLAST15

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem II, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003164911 – 347Photosystem II protein D1UniRule annotationAdd BLAST347

Post-translational modificationi

Tyr-163 forms a radical intermediate that is referred to as redox-active TyrZ, YZ or Y-Z.UniRule annotation

Interactioni

Subunit structurei

PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex and a large number of cofactors. It forms dimeric complexes.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9TM69
SMRiQ9TM69
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 267Quinone (B)UniRule annotation2

Sequence similaritiesi

Belongs to the reaction center PufL/M/PsbA/D family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.85.10, 1 hit
HAMAPiMF_01379 PSII_PsbA_D1, 1 hit
InterProiView protein in InterPro
IPR036854 Photo_II_D1/D2_sf
IPR000484 Photo_RC_L/M
IPR005867 PSII_D1
PANTHERiPTHR33149 PTHR33149, 1 hit
PfamiView protein in Pfam
PF00124 Photo_RC, 1 hit
PRINTSiPR00256 REACTNCENTRE
SUPFAMiSSF81483 SSF81483, 1 hit
TIGRFAMsiTIGR01151 psbA, 1 hit
PROSITEiView protein in PROSITE
PS00244 REACTION_CENTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q9TM69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNTYRASNI SGFNWWGTVI GFILSTSNRF YIGWFGIFMF PLTGLAIIAY
60 70 80 90 100
VAAFILAPPV DIDGIREPVA GSLLYGNNII SGAVIPSSNA IGVHFYPEWE
110 120 130 140 150
SATLLEWLYN GGTYQFVVLH FIGGVSSWMG REWEFSFRLG MRPWIYLAFS
160 170 180 190 200
APLVAATAVF VFYPIGQGSF SDGMPLGVSG TFNFMLVFQA EHNILMHPFH
210 220 230 240 250
ILGVAGVFGG SLFSAMHGSL VTSSLLAETS GDVSLNVGYN FGQEDETYSI
260 270 280 290 300
SAAHGYFGRL IFQYASFNNS RSLHFFLAAW PVIGIWFTAL GVSTMAFNLN
310 320 330 340
GLNFNQSIID SNGHLINSWA DLVNRANLGI EVMHERNTHH YPLDLGL
Length:347
Mass (Da):38,230
Last modified:May 1, 2000 - v1
Checksum:iB3FA659292EE33C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025589 Genomic DNA Translation: BAA83816.2

Similar proteinsi

Entry informationi

Entry nameiPSBA_ALETA
AccessioniPrimary (citable) accession number: Q9TM69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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